| 大熊猫源停乳链球菌类马亚种的分离与鉴定 |
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| 引用本文： | 王旭,颜其贵,陈世界,张和民,李德生,王承东,杨晓龙,雷燕,左兰.大熊猫源停乳链球菌类马亚种的分离与鉴定[J].中国兽医科学,2011(5). |
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| 作者姓名： | 王旭 颜其贵 陈世界 张和民 李德生 王承东 杨晓龙 雷燕 左兰 |
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| 作者单位： | 四川农业大学动物生物技术中心;动物疫病与人类健康四川省重点实验室;四川出入境检验检疫局;中国保护大熊猫研究中心;西南民族大学; |
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| 基金项目： | 教育部“长江学者和创新团队发展计划”创新团队项目(IRT0848); 科技部公益性行业科研专项(2009424188) |
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| 摘 要： | 为研究引起大熊猫脚掌化脓感染的原因,有效防治该病,对大熊猫感染部位的浓汁进行了细菌学检查及分离,对分离菌进行形态特征、理化特性鉴定、兰氏分群以及16S rDNA序列的分子鉴定,并构建系统发育树;对分离菌进行健康小鼠的人工感染试验。结果分离到5株细菌,根据5株分离菌的形态特征、理化特性及兰氏分群结果,结合16S rDNA序列测定与系统发育分析结果,判定为链球菌属的停乳链球菌类马亚种。该分离菌对供试健康小鼠有较强的致病作用。证实分离菌即为引起大熊猫脚掌化脓感染的病原菌。
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| 关 键 词： | 大熊猫 停乳链球菌 分离鉴定 致病性 |
Isolation and identification of a strain ofStreptococcus dysgalactiae subsp. equisimilisfrom giant panda(Ailuropoda melanoleuca) |
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| WANG Xu,YAN Qi-gui,CHEN Shi-jie,ZHANG He-min,LI De-sheng,WANG Cheng-dong,YANG Xiao-long,LEI Yan,ZUO Lan.Isolation and identification of a strain ofStreptococcus dysgalactiae subsp. equisimilisfrom giant panda(Ailuropoda melanoleuca)[J].Veterinary Science in China,2011(5). |
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| Authors: | WANG Xu YAN Qi-gui CHEN Shi-jie ZHANG He-min LI De-sheng WANG Cheng-dong YANG Xiao-long LEI Yan ZUO Lan |
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| Institution: | WANG Xu1,YAN Qi-gui1,2,CHEN Shi-jie3,ZHANG He-min4,LI De-sheng4,WANG Cheng-dong4,YANG Xiao-long5,LEI Yan1,ZUO Lan1(1.Animal Biotechnology Center,Sichuan Agricultural University,Ya'an 625014,China,2.Key Laboratory of Animal Disease and Human Health of Sichuan Province,3.Sichuan Entry-Exit Inspection and Quarantine Bureau,Chengdu 610041,4.China Conservation and Research Center for the Giant Panda,Ya'an 625000,5.Southwest University for Nationalities,China) |
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| Abstract: | In order to investigate the cause of the suppurative infection of giant panda's feet and help for the prevention and treatment of this disease,pathogenic bacteria from pus were examined and isolated.The identifications for the isolated bacteria including morphological characteristics,physiological and biochemical properties,lancefield grouping and 16S rDNA gene sequence were conducted.Phylogenetic tree construction and experimental infection test in healthy mice with the isolated bacteria were also conducte... |
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| Keywords: | giant panda(Ailuropoda melanoleuca) Streptococcus dysgalactiae isolation and identification pathogenicity |
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