| 南京某集贸市场淡水鱼感染嗜水气单胞菌的鉴定及毒力因子检测 |
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| 引用本文： | 宋浩刚,吴亚峰,沈剑强,胡萌,刘永杰,陆承平.南京某集贸市场淡水鱼感染嗜水气单胞菌的鉴定及毒力因子检测[J].中国兽医科学,2011(2):111-115. |
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| 作者姓名： | 宋浩刚 吴亚峰 沈剑强 胡萌 刘永杰 陆承平 |
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| 作者单位： | 南京农业大学动物医学院; |
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| 基金项目： | 教育部“新世纪优秀人才支持计划”项目(NCET-07-0440); 公益性行业(农业)科研专项(200803013); 南京农业大学SRT项目(0904A16) |
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| 摘 要： | 从南京市某集贸市场所售6种淡水鱼体内分离细菌,根据细菌染色、培养特性及16 S rRNA基因扩增等特点进行嗜水气单胞菌的鉴定,对鉴定阳性的菌株测定其溶血、溶蛋白特性,采用PCR技术调查毒力因子的基因分布情况,以斑马鱼作为实验动物研究了不同毒力基因型代表菌株的致病性.试验共分离得到39株嗜水气单胞菌,检出率为65.0%(...
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| 关 键 词： | 嗜水气单胞菌 毒力因子 半数致死量 集贸市场 南京 |
Identification and detection of virulence factors of Aeromonas hydrophila in freshwater fish from a market in Nanjing |
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| SONG Hao-gang,WU Ya-feng,SHEN Jian-qiang,HU Meng,LIU Yong-jie,LU Cheng-ping.Identification and detection of virulence factors of Aeromonas hydrophila in freshwater fish from a market in Nanjing[J].Veterinary Science in China,2011(2):111-115. |
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| Authors: | SONG Hao-gang WU Ya-feng SHEN Jian-qiang HU Meng LIU Yong-jie LU Cheng-ping |
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| Institution: | SONG Hao-gang,WU Ya-feng,SHEN Jian-qiang,HU Meng,LIU Yong-jie,LU Cheng-ping(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China) |
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| Abstract: | Aeromonas hydrophila was isolated and identified in the freshwater fish from a market in Nanjing on the basis of bacterial staining,culture characteristics and 16 S rRNA gene amplification.For the identified strains,the hemolytic and proteolytic activities of extracellular products were tested and analyzed with respect to the prevalence of the aerolysin(aerA),cytotoxic enterotoxin(act),cytotonic enterotoxin(alt) and serine protease(ahp) and thermo-sensitive extracellular proteinase(eprCAI) genes by PCR assa... |
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| Keywords: | Aeromonas hydrophila virulence factor LD50 market Nanjing |
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