| 供体细胞形态、直径和传代次数对猪体细胞核移植效果的影响 |
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| 引用本文： | 黄雅琼,石德顺,陈旭健,李家洲,曾诗媛,詹萍,阮桂文,赵仕花.供体细胞形态、直径和传代次数对猪体细胞核移植效果的影响[J].中国兽医科学,2011(5). |
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| 作者姓名： | 黄雅琼 石德顺 陈旭健 李家洲 曾诗媛 詹萍 阮桂文 赵仕花 |
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| 作者单位： | 玉林师范学院;广西大学动物繁殖研究所; |
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| 基金项目： | 广西自然科学基金项目(2010GXNSFA013105); 广西壮族自治区教育厅科研项目(200911MS203); 玉林师范学院重点项目(2009YJZD01); 国家高技术研究发展计划(863)项目(2007AA100505,2008AA101006) |
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| 摘 要： | 通过采集猪卵巢表面的卵母细胞,经过体外成熟和去核,供体细胞经过分离、培养和传代,注核并构建重构胚胎,融合和激活重构胚胎,以探讨供体细胞的形态、直径和传代次数对猪体细胞核移植重构胚胎的发育能力和核移植效果的影响。结果显示,表面光滑的卵丘细胞作为供体细胞核移植后的分裂率和囊胚率均显著高于表面粗糙的卵丘细胞(P<0.05),虽然融合率差异不显著(P>0.05)。直径小于15μm的供体细胞核移植后的融合率显著低于直径大于30μm的供体细胞(P<0.05)。直径20～30μm供体细胞核移植后的分裂率与直径小于15μm的供体细胞组的分裂率差异显著(P<0.05),与直径大于30μm的供体细胞组差异不显著(P>0.05)。直径20～30μm的供体细胞核移植后的分裂率与囊胚率(63.73%和21.54%)较高,囊胚率有显著差异(P<0.05)。对不同代数胎儿成纤维细胞核移植效果的研究发现,6～9代成纤维细胞的融合率显著高于3～5代和≥10代的成纤维细胞(P<0.05),但卵裂率和囊胚率差异不显著(P>0.05)。结果表明,表面光滑的供体细胞有利于重构胚胎的发育,直径20～30μm的供体细胞较适合进行核移植,用传代培养6～9代...
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| 关 键 词： | 猪卵母细胞 体细胞核移植 供体 重构胚 效果 |
Influence of morphology,diameter and passage of the donor cells on porcine somatic cell nuclear transfer |
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| HUANG Ya-qiong,SHI De-shun,CHEN Xu-jian,LI Jia-zhou,ZENG Shi-yuan,ZHAN Ping,RUAN Gui-wen,ZHAO Shi-hua.Influence of morphology,diameter and passage of the donor cells on porcine somatic cell nuclear transfer[J].Veterinary Science in China,2011(5). |
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| Authors: | HUANG Ya-qiong SHI De-shun CHEN Xu-jian LI Jia-zhou ZENG Shi-yuan ZHAN Ping RUAN Gui-wen ZHAO Shi-hua |
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| Institution: | HUANG Ya-qiong1,2,SHI De-shun2,CHEN Xu-jian,LI Jia-zhou1,ZENG Shi-yuan1,ZHAN Ping1,RUAN Gui-wen1,ZHAO Shi-hua1(1.Yulin Normal University,Yulin 537000,China,2.Institute of Animal Reproduction,Guangxi University,Nanning 530005,China) |
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| Abstract: | Oocytes on the surface of porcine ovaries were collected,in vitro matured and enucleated.Donor cells were dissected,cultured and passaged.The donor nucleus was transfered into the enucleated oocyte to reconstruct an embryo.The reconstructed embryos were fused and activated in order to discuss the developmental potential of the reconstructed embryos in the morphology and diameter and passage of donor cells and explore the transfer effect in the porcine somatic cells nuclear transfer.In result,the cleavage ra... |
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| Keywords: | porcine oocyte somatic cell nuclear transfer donor reconstructed embryos effect |
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