| 流产布鲁氏菌bp26和omp10基因的同源性分析 |
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| 引用本文： | 王军,王瑞,申之义,杨莲茹.流产布鲁氏菌bp26和omp10基因的同源性分析[J].中国兽医科学,2011(1):25-30. |
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| 作者姓名： | 王军 王瑞 申之义 杨莲茹 |
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| 作者单位： | 内蒙古大学生命科学学院;内蒙古农业大学兽医学院; |
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| 基金项目： | 内蒙古自然科学基金资助项目(200508010412) |
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| 摘 要： | 利用布鲁氏菌外膜蛋白bp26基因和omp10基因,对分离的布鲁氏菌和参考布鲁氏菌菌株进行克隆和序列分析,以明确分离的布鲁氏菌和参考布鲁氏菌菌株与基因库中的布鲁氏菌外膜蛋白bp26基因和omp10基因间的同源性.根据GenBank中的布鲁氏菌bp26基因和omp10基因,分别设计合成了1对特异性引物,以提取的分离株布鲁氏...
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| 关 键 词： | 布鲁氏菌 bp26基因 omp10基因 同源性 分析 |
Identity analysis of bp26 and omp10 genes of Brucella abortus |
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| WANG Jun,WANG Rui,SHEN Zhi-yi,YANG Lian-ru.Identity analysis of bp26 and omp10 genes of Brucella abortus[J].Veterinary Science in China,2011(1):25-30. |
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| Authors: | WANG Jun WANG Rui SHEN Zhi-yi YANG Lian-ru |
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| Institution: | WANG Jun1,WANG Rui2,SHEN Zhi-yi2,YANG Lian-ru2(1.College of Life Science,Inner Mongolia University,Hohhot 010021,China,2.College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China) |
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| Abstract: | The aim of the present study was to analyze the identity of genes coding for Brucella outer membrane proteins BP26 and OMP10 among Brucella of testing or reference strains and GenBank strains.Two pairs of primers specific to Brucella bp26 and omp10 genes were designed and synthesized according to GenBank sequences,respectively.bp26 and omp10 genes were amplified by PCR from the genomic DNAs extracted from testing or 2 reference Brucella strains.The PCR products were cloned into the pMD18-T vector and sequen... |
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| Keywords: | Brucella bp26 gene omp10 gene identity analysis |
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