| 冬虫夏草菌丝水提液对SP2/0细胞凋亡的影响 |
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| 引用本文： | 刘彦威,刘娜,苏敬良,韩博,田向荣.冬虫夏草菌丝水提液对SP2/0细胞凋亡的影响[J].中国兽医科学,2006,36(9):729-732. |
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| 作者姓名： | 刘彦威 刘娜 苏敬良 韩博 田向荣 |
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| 作者单位： | 1. 河北工程大学,农学院,河北,邯郸,056021
2. 中国农业大学,动物医学院,北京,100094 |
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| 摘 要： | 在冬虫夏草菌丝水提液诱导下,采用Hoechst33342荧光染色、MTT、琼脂糖凝胶电泳和流式细胞仪等方法,观察和分析了SP2/0细胞的凋亡情况。结果显示,冬虫夏草菌丝水提液可抑制SP2/0细胞增殖,在荧光显微镜下,凋亡细胞呈亮绿色,DNA琼脂糖电泳可见典型的“阶梯状”条带,Annexin-V-FITC和碘化丙啶(PI)双染后流式细胞仪分析显示,细胞凋亡数量明显增多。表明,冬虫夏草菌丝水提液对SP2/0细胞增殖的抑制作用可能与诱导其凋亡有关。
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| 关 键 词： | 冬虫夏草 菌丝水提液 骨髓瘤细胞 凋亡 |
| 文章编号： | 1673-4696(2006)09-0729-04 |
| 修稿时间： | 2006年4月5日 |
Effect of water extract from mycelium of Cordyceps sinensis on apoptosis of SP2/0 cells |
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| LIU Yan-wei,LIU Na,SU Jing-liang,HAN Bo,TIAN Xiang-rong.Effect of water extract from mycelium of Cordyceps sinensis on apoptosis of SP2/0 cells[J].Veterinary Science in China,2006,36(9):729-732. |
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| Authors: | LIU Yan-wei LIU Na SU Jing-liang HAN Bo TIAN Xiang-rong |
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| Abstract: | MTT assay,agarose gel electrophoresis,flow cytometric analysis and Hoechst33342 staining were performed to study apoptosis and its mechanism.After SP2/0 cells were treated with water extract of mycelium,growth of SP2/0 cells was inhibited and SP2/0 cells appeared lighter green in fluorescence microscope.A typical DNA ladder was detected by agarose gel electrophoresis of DNA extracted from SP2/0 cells.Flow cytometric analysis of SP2/0 cells by Annexin-V-Fluos and propidium iodide double staining demonstrated that quantity of apoptosis cell increased with water extract induction.The water extract of mycelium induced apoptosis in SP2/0 cells,indicating that growth of SP2/0 cells was inhibited.The mechanism of anti-tumor activity of water extract of mycelium may be related to induction of apoptosis in tumor cells. |
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| Keywords: | Cordyceps sinensis water extract from mycelium myeloma cells apoptosis |
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