九种中药成分对体外培养小鼠淋巴细胞功能的影响

为进一步探索黄芪多糖等9种中药成分免疫增强活性的作用机理并比较各成分之间免疫增强活性的强弱,在体内试验的基础上,用脾淋巴细胞增殖反应和抗体夹心酶联免疫吸附试验测定了它们对体外培养的小鼠淋巴细胞功能的影响。结果表明,人参皂甙、蜂胶黄酮、黄芪多糖、淫羊藿黄酮、淫羊藿多糖都能显著地直接或协同ConA刺激脾和外周血淋巴细胞增殖,也能显著增加LPS诱导的脾淋巴细胞增殖活性和显著升高体外培养的小鼠脾淋巴细胞产生的IgG水平;当归多糖能协同ConA和LPS的诱导活性,提高小鼠脾淋巴细胞体外培养系统的IgG水平;黄芪皂甙能直接和协同LPS刺激淋巴细胞增殖,板蓝根多糖则仅能促进LPS的诱导活性,蜂胶多糖的作用则均不明显。

Effects of 9 Chinese herbal ingredients on function of lymphocytes of mice in vitro

To study immune activities of nine Chinese herbal ingredients(CHIs),astragalus polysaccharide(APS),propolis polysaccharide(PPS),epimedium polysaccharide(EPS),Chinese angelica polysaccharide(CAPS),isatis root polysaccharide(IRPS),propolis flavone(PF),epimedium(flavone(EF)),ginsenoside(GS) and astragaloside(AS),effects of these CHIs on lymphocytes of mice in vitro were compared by the methods of MTT assay and sandwich ELISA.GS,PF,APS,EF and EPS stimulated lymphocytes significantly,which were induced by LPS and/or ConA,to proliferate and elevated significantly level of antibody IgG produced by splenocytes in vitro.CAPS had the same effects but failed to stimulated lymphocytes to proliferate independently.AS and IRPS stimulated significantly lymphocytes which were inducd by LPS to proliferate.The results suggested that these CHIs have different effects on the functions of lymphocytes of mice in vitro.