| H5N1和H9N2亚型禽流感病毒型特异性抗原捕捉ELISA检测方法的建立 |
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| 引用本文： | 宋建领,张富强,胡媛媛,王金萍.H5N1和H9N2亚型禽流感病毒型特异性抗原捕捉ELISA检测方法的建立[J].中国兽医科学,2005,35(11):879-882. |
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| 作者姓名： | 宋建领 张富强 胡媛媛 王金萍 |
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| 作者单位： | 云南省热带亚热带动物病毒病重点实验室,云南,昆明,650224 |
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| 基金项目： | 云南省科技攻关计划项目(2004NG-01)，农业部“948”项目(2004-Z41) |
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| 摘 要： | 采用禽流感病毒多克隆抗体及型特异性单克隆抗体,研究建立了型特异性抗原捕捉ELISA检测方法,用于检测H5N1和H9N2亚型禽流感病毒。优化了反应条件,确定了包被抗体、检测抗体及酶结合物的最佳工作浓度,对该方法的敏感性、特异性、重复性及稳定性进行了分析,并与病毒分离鉴定的结果进行了比较。结果表明,该方法敏感、特异,具有良好的重复性和稳定性,可用于临床样品及鸡胚、细胞培养物中禽流感病毒的检测。
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| 关 键 词： | 禽流感病毒 型特异性 抗原捕捉ELISA |
| 文章编号： | 1000-6419(2005)11-0879-04 |
| 修稿时间： | 2005年7月27日 |
Development of type-specific antigen capturer ELISA for detection of H5N1 and H9N2 subtype avian influenza viruses |
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| SONG Jian-ling,ZHANG Fu-qiang,HU Yuan-yuan,WANG Jin-ping.Development of type-specific antigen capturer ELISA for detection of H5N1 and H9N2 subtype avian influenza viruses[J].Veterinary Science in China,2005,35(11):879-882. |
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| Authors: | SONG Jian-ling ZHANG Fu-qiang HU Yuan-yuan WANG Jin-ping |
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| Abstract: | By using avian influenza polyclonal antibodies and type-specific monoclonal antibodies,a type specific antigen capture ELISA was developed for detection of H5N1 and H9N2 subtype avian influenza(viruses).The reaction condition and the working concentrations of coating antibody,test antibody and(enzyme-)labeled conjugate were optimized.The sensitivity,specificity,repeatability and stability of the assay were analyzed and compared with virus isolation and identification.Results indicated that the antigen capture ELISA was sensitive,specific,with high repeatability and stability for detection of avian influenza virus in clinical samples,embryo and cell culture tissues. |
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| Keywords: | avian influenza virus type-specific antigen capture ELISA |
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