ELISA抗体水平评价口蹄疫灭活疫苗免疫效果的可行性分析

为了探讨免疫抗体评价口蹄疫灭活疫苗效果的可行性,分别用5批次的口蹄疫O型-亚洲1型二价灭活疫苗(OS/99株+JSL/06株)、5批次的猪口蹄疫O型灭活疫苗(OZK/93株+OS/99株)免疫动物,在攻毒的同时采血分离血清,用液相阻断ELISA测定抗体效价,以分析抗体效价与免疫保护的关系。统计学分析表明,二价灭活疫苗的O型免疫抗体值与攻毒保护率(概率单位)之间呈正相关关系(P<0.01),相关系数为0.986;亚洲1型免疫抗体值与攻毒保护率(概率单位)之间呈正相关关系(P<0.05),相关系数为0.997,猪口蹄疫O型灭活疫苗免疫抗体值与攻毒保护率(概率单位)之间不呈正相关关系(P>0.05)。结果表明,应用抗体水平评价二价灭活疫苗的免疫效果是完全可行的,但用其评价猪O型灭活疫苗的免疫效果不具有可行性。

Feasibility study of the effect of foot-and-mouth disease inactivated vaccine by antibody titer evaluation

In order to evaluate the feasibility of foot-and-mouth disease vaccine according to antibody level,five batches of inactivated vaccine against foot-and-mouth disease(FMD) type O & Asia 1 and five batches of inactivated vaccine against swine FMD type O were inoculated to the animals.While the relationship between antibody titers and immune protection percentages was evaluated by liquid blocking ELISA.The statistical data proved that the O antibody titer of the inactivated vaccine against FMD type O & Asia 1 was positively correlated with the protection percentages(probability units) post-challenge(P<0.01,r=0.986).Asia 1 antibody titer of the inactivated vaccine against FMD type O & Asia 1 was positively correlated(P<0.05,r=0.997) with the percentages of protection(probability units) post-challenge,but O antibody titers of the inactivated vaccine against swine FMD type O were not positive correlated(P>0.05) with the percentage of protection(probability units) post-challenge.The result indicated that it is entirely feasible to evaluation of effectiveness of the inactivated vaccine against FMD type O & Asia 1 using antibody titers,and not feasible to evaluation of effectiveness of the inactivated vaccine against FMD type O swine using antibody titers.