| 山羊痘病毒的鉴定及序列分析 |
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| 引用本文： | 张克山,郑海学,靳野,何继军,刘永杰,尚佑军,郭建宏,卢国栋,田宏,刘湘涛.山羊痘病毒的鉴定及序列分析[J].中国兽医科学,2011(12). |
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| 作者姓名： | 张克山 郑海学 靳野 何继军 刘永杰 尚佑军 郭建宏 卢国栋 田宏 刘湘涛 |
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| 作者单位： | 中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部畜禽病毒学重点开放实验室国家口蹄疫参考实验室; |
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| 基金项目： | 国家现代肉羊产业技术体系项目(CARS-39); “十一五”国家科技支撑计划项目(2006BAD06A17) |
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| 摘 要： | 以华中地区某山羊场疑似山羊痘病料为对象,进行了本动物病例复制、透射电镜观察、病理切片HE染色、特异性目的基因扩增和序列测定及遗传进化关系分析。结果,复制了山羊痘本动物模型,观察到山羊痘病毒(GTPV)样粒子,切片HE染色可见有炎性细胞浸润,病料中扩增到P32基因;序列测定及遗传分析表明,该病毒为山羊痘病毒(ZL/HB/HN),与2008年分离于印度的山羊痘毒株(FJ748488)的遗传关系最近,与中国山羊痘疫苗株(AY881707)及1978年分离于印度的山羊毒株(AY382869)同属一个进化分支。
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| 关 键 词： | 山羊痘 本动物病例复制 病毒鉴定 序列分析 |
Goatpox animal model replication,virus identification and sequence analysis |
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| ZHANG Ke-shan,ZHENG Hai-xue,JIN Ye,HE Ji-jun,LIU Yong-jie,SHANG You-jun,GUO Jian-hong,LU Guo-dong,TIAN Hong,LIU Xiang-tao.Goatpox animal model replication,virus identification and sequence analysis[J].Veterinary Science in China,2011(12). |
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| Authors: | ZHANG Ke-shan ZHENG Hai-xue JIN Ye HE Ji-jun LIU Yong-jie SHANG You-jun GUO Jian-hong LU Guo-dong TIAN Hong LIU Xiang-tao |
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| Institution: | ZHANG Ke-shan,ZHENG Hai-xue,JIN Ye,HE Ji-jun,LIU Yong-jie,SHANG You-jun,GUO Jian-hong,LU Guo-dong,TIAN Hong,LIU Xiang-tao(Lanzhou Veterinary Research Institute,Chinese Academy of Agriculture Science/State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Animal Virology of the Ministry of Agriculture/National Foot-and-Mouth Disease Reference Laboratory,Lanzhou 730046,China) |
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| Abstract: | Crust materials with clinical sore from a farm in Huazhong region were used to identify suspected goatpox disease case and analyse it's molecular characteristics,GTPV was confirmed by animal model replication,projection electron microscope,HE staining and specific gene(P32) PCR amplification.Sequence analysis of GTPV strain was performed using amino acid sequence homology comparison and phylogenetic analysis based on P32 gene.In result,this disease case was caused by GTPV(ZL/HB/HN).Phylogenetic tree analysi... |
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| Keywords: | goatpox animal model replication virus identification sequence analysis |
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