| 家畜附红细胞体病免疫学诊断方法的研究进展 |
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| 引用本文： | 顾有方,李文超,陈会良,刘德义.家畜附红细胞体病免疫学诊断方法的研究进展[J].中国兽医科学,2007,37(5):456-460. |
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| 作者姓名： | 顾有方 李文超 陈会良 刘德义 |
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| 作者单位： | 安徽科技学院,动物科学学院,安徽,凤阳,233100 |
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| 基金项目： | 安徽省教育厅自然科学基金重点项目 |
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| 摘 要： | 概述了补体结合试验、荧光抗体技术、间接血凝试验和酶联免疫吸附试验等用于诊断家畜附红细胞体病的血清学方法的建立和应用情况,同时简述了家畜附红细胞体的分子生物学检测技术,这些技术包括DNA探针技术、常规PCR和实时定量PCR。指出了上述各种诊断方法存在的不足,期待这些方法的进一步完善和规范。
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| 关 键 词： | 附红细胞体病 免疫学诊断方法 家畜 |
| 文章编号： | 1673-4696(2007)05-0456-05 |
| 修稿时间： | 2007年1月16日 |
Advance in immunological diagnostic methods of eperythrozoonosis in domestic animals |
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| GU You-fang,LI Wen-chao,CHEN Hui-liang,LIU De-yi.Advance in immunological diagnostic methods of eperythrozoonosis in domestic animals[J].Veterinary Science in China,2007,37(5):456-460. |
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| Authors: | GU You-fang LI Wen-chao CHEN Hui-liang LIU De-yi |
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| Abstract: | The development and application of complement fixation test(CFT),indirect fluorescent-antibody assay(IFA),indirect hemagglutination assay(IHA) and enzyme-linked immunosorbent assay(ELISA) were reviewed for the detection of eperythrozoonosis in domestic animals.Molecular biological methods including DNA probe,routine PCR and real-time quantitative PCR for the detection of eperythrozoonosis were introduced briefly too.The shortcomings existing in the above-mentioned diagnostic me-thods were pointed out,and the me-thods would be expected to be improved and standardized. |
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| Keywords: | eperythrozoonosis immunological diagnostic method domestic animals |
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