猪水泡病病毒结构蛋白基因的克隆及其蛋白二级结构和淋巴细胞表位的预测

以免疫信息学和反向疫苗学为理论根据,利用RT-PCR法获得了猪水泡病病毒(SVDV)HK/70株的基因组序列并测序,应用生物信息学相关软件和方法,对SVDV结构蛋白的二级结构的不同方面及其抗原特异性淋巴细胞表位进行了预测,综合评价了SVDV结构蛋白的抗原特异性细胞表位,并计算出一些潜在的抗原位点。结果表明,VP1蛋白含有的细胞优势抗原表位最多,但其他结构蛋白也含有抗原特异性淋巴细胞表位,有的甚至有可能成为优势抗原表位或对优势抗原表位有协同作用。

Cloning of SVDV structural protein gene and predictions of secondary structure and lymphocyte epitopes of structural protein

On the basis of immunological bioinformatics and reverse vaccinology,the structural protein(VP1) gene of the swine vesicular disease virus(SVDV) HK/70 strain was amplified by RT-PCR and then sequenced.The secondary structure of the SVDV VP1 and its lymphocyte epitopes with specificity of antigen were predicted and evaluated comprehensively,and some potential antigenic determinants were worked out from the VP1 gene sequence of the strain,with related softwares and methods of bioinformatics.Results showed that the potential antigenic determinants and lymphocyte epitopes were the most in the structural protein VP1,and VP1 should have immunogenicity and could be selected for development of gene engineering vaccines.But the other structural proteins also contained determinants and epitopes,and may be main determinants and epitopes.