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| 猪胚胎多能性干细胞的分离培养 | |
| 引用本文： | 况玲,冯书堂,余四九,牟玉莲,张勇.猪胚胎多能性干细胞的分离培养[J].中国兽医科学,2004,34(7):55-59. |
| 作者姓名： | 况玲 冯书堂 余四九 牟玉莲 张勇 |
| 作者单位： | 1. 甘肃农业大学,动物医学院,甘肃,兰州,730070;中国农业科学院,畜牧研究所,北京,100094 2. 中国农业科学院,畜牧研究所,北京,100094 3. 甘肃农业大学,动物医学院,甘肃,兰州,730070 |
| 基金项目： | 国家自然科学基金重大项目 (NO .339990 342 0 ) |
| 摘 要： | 收集妊娠 2 6 .0～ 2 9.0d的猪胚胎 ,分离培养原始生殖细胞 (PGCs) ,在STO细胞饲养层上生长 ,形成了多能性干细胞 (EG)。发现其具有干细胞的显著特性 ,并能在体外定向分化为神经细胞、上皮细胞和胚泡细胞。试验使用A、B、C 3种不同的培养基培养PGCs ,结果在形成EG细胞集落的数量和EG细胞分化上有着明显的差距 ,说明血清的质量和浓度以及细胞因子对干细胞的生成和增殖有着重要的影响 |
| 关 键 词： | 胚胎干细胞 原始生殖细胞 胎牛血清 细胞因子 |
| 文章编号： | 1000-6419(2004)07-0055-05 |
| 修稿时间： | 2004年5月11日 |
Isolation of pluripotent stem cells from cultured porcine germ cells |
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| KUANG Ling.Isolation of pluripotent stem cells from cultured porcine germ cells[J].Veterinary Science in China,2004,34(7):55-59. | |
| Authors: | KUANG Ling |
| Institution: | KUANG Ling~ |
| Abstract: | Embryonic germ(EG) cells are undifferentiated stem cells isolated from cultured primordial germ cells( PGCs). PGCs collected from day 26 or 29 porcine embryos were cultured on mitotically inactivated murine fibroblasts(STO). EG cells share several characteristics with embryonic stem(ES) cells, (including) morphology, pluripotency and the capacity for germline transmission. EG cells possessed alkaline phosphatase activity,and differentiated into several cell types in vitro. PGCs were cultured respectively with the different culture medium. These results suggested that the quality and concentration of serum ,and addition of growth factors are essential for the in vitro survival and proliferation of cultured porcine PGC. |
| Keywords: | embryonic stem cell primordial germ cell fatal bovine serum growth factor |
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