| 不同家禽红细胞悬液对禽流感血清抗体检测的影响 |
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| 引用本文： | 张评浒,唐应华,仇旭升,薛峰,曹永忠,高崧,刘秀梵.不同家禽红细胞悬液对禽流感血清抗体检测的影响[J].中国兽医科学,2005,35(4):267-271. |
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| 作者姓名： | 张评浒 唐应华 仇旭升 薛峰 曹永忠 高崧 刘秀梵 |
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| 作者单位： | 扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 |
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| 基金项目： | 国家科技攻关计划项目(2004BA519A02) |
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| 摘 要： | 为探讨不同家禽红细胞对禽流感血清抗体检测的影响,对不同家禽的禽流感阳性血清分别经不同方法处理后,做了一系列对比试验。结果发现,经受体破坏酶(RDE)处理后,虽然可以消除异种家禽血清对异种家禽红细胞的非特异性凝集作用的影响,但因处理复杂,费用高,不宜推广应用;经鸡红细胞吸附处理,可明显降低水禽血清对异种红细胞的非特异性凝集作用,但处理费时;而用水禽红细胞检测同种水禽的禽流感血清抗体,可以有效去除血清非特异性凝集的影响,且更能准确地反映水禽经禽流感疫苗免疫后的抗体水平。因而,应选用与宿主来源相同的红细胞悬液检测水禽禽流感血清抗体。
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| 关 键 词： | 禽流感 红细胞 血凝 血凝抑制 异种家禽血清 |
| 文章编号： | 1000-6419(2005)04-0267-05 |
| 修稿时间： | 2004年10月17 |
Influence of different avian red blood cells on detection of HI antibody against avian influenza |
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| ZHANG Ping-hu,TANG Ying-hua,QIU Xu-sheng,XUE Feng,CAO Yong-zhong,GAO Song,LIU Xiu-fan.Influence of different avian red blood cells on detection of HI antibody against avian influenza[J].Veterinary Science in China,2005,35(4):267-271. |
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| Authors: | ZHANG Ping-hu TANG Ying-hua QIU Xu-sheng XUE Feng CAO Yong-zhong GAO Song LIU Xiu-fan |
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| Abstract: | The influence of non-special haemagglutinin upon HI test of different avian sera was investigated by different treatment of the sera and serial experiments. The results showed that non-special hae- magglutinin disappeared, when the different avian sera was treated by receptor-destroyed enzyme(RDE) or chicken red blood cells, but the RDE was too expensive and the treatment of chicken red blood cells was time-consuming. |
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| Keywords: | avian influenza red blood cell haemagglutination haemagglutination inhibition non-special avian serum |
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