| 小鹅瘟病毒荧光抗体的制备与应用 |
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| 引用本文： | 郑大恒,李永明,温贵兰,朱德全,文明.小鹅瘟病毒荧光抗体的制备与应用[J].中国兽医科学,2003,33(11):33-36. |
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| 作者姓名： | 郑大恒 李永明 温贵兰 朱德全 文明 |
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| 作者单位： | 贵州大学,动物科学学院,动物医学系,贵州,贵阳,550025 |
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| 摘 要： | 将小鹅瘟病毒 (GPV )分离毒xw株接种健康兔获得兔抗GPV超免疫血清 ,由此制备兔抗GPV荧光抗体。采用直接荧光抗体试验对人工感染雏鹅、鹅胚及自然病例进行了检测。结果表明 ,该方法可检出患病组织中的病毒抗原 ,且肠和肝的含毒量高于尿囊细胞。
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| 关 键 词： | 小鹅瘟病毒 荧光抗体 检测 抗原 |
| 文章编号： | 1000-6419(2003)11-0033-04 |
| 修稿时间： | 2003年7月16日 |
Preparation and application of fluorescent antibody of gosling plague virus |
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| ZHENG Da-heng,LI Yong-ming,WEN Gui-lan,ZHU De-quan,WEN Ming.Preparation and application of fluorescent antibody of gosling plague virus[J].Veterinary Science in China,2003,33(11):33-36. |
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| Authors: | ZHENG Da-heng LI Yong-ming WEN Gui-lan ZHU De-quan WEN Ming |
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| Abstract: | Gosling plague virus (GPV) was injected into health rabbits so as to make rabbit anti-GPV hyperimmune sera and to prepare fluorescent antibodies. The artificial infectious goslings, embryos and natural cases were detected by direct fluorescent antibody test .The results showed that this method can detect the viral antigen of affected tissues. The virus contents in intestine and liver were much more than that in allantoic cells. |
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| Keywords: | gosling plague virus fluorescent antibody detection antigen |
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