| 大熊猫等八种野生珍稀动物蛔虫ITS-2基因的序列分析 |
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| 引用本文： | 何光志,牛李丽,杨光友,邓家波,王帅,余星明,汪涛,古小彬,陈维刚.大熊猫等八种野生珍稀动物蛔虫ITS-2基因的序列分析[J].中国兽医科学,2008,38(11). |
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| 作者姓名： | 何光志 牛李丽 杨光友 邓家波 王帅 余星明 汪涛 古小彬 陈维刚 |
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| 作者单位： | 1. 四川农业大学动物医学院,四川雅安,625014
2. 成都动物园,四川成都,610081 |
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| 基金项目： | 四川省成都市大熊猫繁育研究基金 |
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| 摘 要： | 为了探讨寄生在大熊猫、小熊猫、黑猩猩、白眉长臂猿和4种熊科动物(北极熊、棕熊西藏亚种、狗熊四川亚种和棕熊东北亚种)体内蛔虫的分类地位,测定了核糖体第二内转录间隔区(ITS-2)基因序列,并对这些序列进行同源性分析和采用UPGMA法构建分子系统树。结果表明:寄生在大熊猫、小熊猫和4种熊科动物体内的蛔虫均为贝蛔属蛔虫;寄生在黑猩猩和白眉长臂猿体内的蛔虫为蛔属蛔虫。分析结果提示蛔虫与宿主之间存在协同进化关系。
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| 关 键 词： | 大熊猫 野生珍稀动物 蛔虫 核糖体ITS-2基因 序列分析 协同进化 |
Sequence analysis of ITS-2 rDNA of roundworms from Ailuropoda melanoleuca and seven rare wild animals |
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| HE Guang-zhi,NIU Li-li,YANG Guang-you,DENG Jia-bo,WANG Shuai,YU Xing-ming,WANG Tao,GU Xiao-bin,CHEN Wei-gang.Sequence analysis of ITS-2 rDNA of roundworms from Ailuropoda melanoleuca and seven rare wild animals[J].Veterinary Science in China,2008,38(11). |
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| Authors: | HE Guang-zhi NIU Li-li YANG Guang-you DENG Jia-bo WANG Shuai YU Xing-ming WANG Tao GU Xiao-bin CHEN Wei-gang |
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| Abstract: | To clarify the taxonomic status of roundworms from Ailuropoda melanoleuca,Ailurus fulgens,Pan troglodytes,Hylobates hoolock and 4 species of ursid(Ursus maritimus,Ursus arctos pruinosus,Selenartos thibetanus mupinensis and Ursus arctos lasiotus),the second internal transcribed spacer 2(ITS-2) rDNA was amplified,sequenced and compared with sequences available in GenBankTM.The phylogenetic trees was constructed based on ITS-2 sequences by UPGMA.The results showed that the roundworms from A.melanoleuca,A.fulgens and 4 species of ursid represent Baylisascaris,and the roundworms from H.hoolock and P.troglodytes represent Ascaris.The results showed that co-evolutionary relationship existed between roundworms and their hosts. |
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| Keywords: | Ailuropoda melanoleuca rare wildlife animal roundworm ribosome ITS-2 rDNA sequence analysis coevolution |
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