Forensic medical nomenclature of human organ and tissue antigens

Development of a new trend in forensic medical is discussed: organ and tissue serology. The author validates the need in development of the relevant taxonomy with consideration for specific terms and items. Data on tissue and organ antigens are classified and a scheme of their classification for forensic medicine is proposed for the first time.     

评人体器官与组织捐赠无偿性原则——兼对器官与组织捐赠有偿化改革的设想

人体器官、组织捐赠无偿性原则是联合国反复重申并被各国立法严格贯彻的医疗及法律准则 ,但该原则亦有贯彻“过度”的问题。而这一问题的解决有赖于对器官、组织捐赠有偿化及其限度的探讨。     

评人体器官与组织捐赠无偿性原则－－兼对器官与组织捐赠有偿化改革的设想

人体器官、组织捐赠无偿性原则是联合国反复重申并被各国立法严格贯彻的医疗及法律准则,但该原则亦有贯彻"过度"的问题.而这一问题的解决有赖于对器官、组织捐赠有偿化及其限度的探讨.     

ABO tissue antigens of Egyptian mummies

ABO groups were investigated on skin (and muscle), bone and hair specimens from 14 Egyptian mummies dating from the Roman period. Samples were tested by the AE (absorption-elution), MA (mixed agglutination) and HIF (histo-immunofluorescence) methods, in order to evaluate the reliability of each method. For half of the mummies (7) the results were concordant on all samples (3-9 samples for each mummy) with all employed methods, suggesting an unequivocal blood group conclusion. For the other seven mummies there were discordant results with the different methods and interpretation of the results was thus inconclusive. HIF seems to be the most reliable method as specific blood group substances are identified on specific histologic structures. Failure to detect tissular ABO antigens was mainly due to excessive resin impregnation.     

Microbial contamination of ABO antigens in bone tissue

Fragments of human bones were stored in different media for two years and then expression of the ABO antigens was indicated. Simultaneously, microbiological investigations were performed. In almost all cases, different ABO substances were detected in putrefied and fresh bones taken from the same person. Blood group antigens found in putrefied bones were compared with serological activity of bacterium cultured from these tissues. Attempts were made to remove unspecific reactions. The authors assume that bacteria are responsible for nonspecific serological reactions, not only as a carrier of blood-group-like substances, but also as a source of enzymes responsible for changes in the structure of ABO antigens in putrefied bones.     

法医器官组织特异性circRNAs的筛选及确证

目的 筛选并确证器官组织特异性circRNAs分子标记物,应用于法医器官组织溯源.方法 从TSCD数据库及文献中,筛选出器官组织特异性circRNAs,设计并验证引物.收集心脏、大脑、肝脏、皮肤、骨骼肌、肺脏等人体组织样本,经总RNA提取、RT-qPCR、琼脂糖凝胶电泳等步骤对候选circRNAs进行组织表达水平分析,...     

Immunochemical and chromatographic determination of ABH antigens in compact bone tissue

The identification of ABH antigens from compact bone tissue is known from many sources. The purpose of this study was to make a contribution to the localization of blood-group-active substances in compact bone tissue. A variety of preparation and identification methods were successfully used and compared. Samples were extracted from compact bone tissue, separated by HPTLC, and examined using the absorption-elution and PAP techniques. Additionally, the PAP technique was carried out on cryostat sections. Serologically active blood-group substances were consistently demonstrated in the organic components of the haversian canals.     

Addition of an adipose marker to the organ tissue identification mRNA assay

At the Netherlands Forensic Institute, mRNA profiling for the purpose of organ tissue identification is frequently requested in casework. The previous version of the assay (denoted Organtyper) lacked markers for identifying adipose tissue, while this was occasionally questioned in casework. Here, three potential adipose markers are examined before incorporating one into the final Organtyper assay. The added value of the adipose marker becomes clear from looking at casework results.     

人体组织/体液来源的法医学鉴定

生物检材的组织/体液来源鉴定是法医学检验的重要内容之一。法医学组织/体液鉴定的方法较多,无论是基于酶促反应或免疫学检测的传统鉴定方法,还是光谱法或近年来基于mRNA、microRNA或DNA甲基化差异的分子生物学组织鉴定技术,均各有特点。本文就各种法医学组织/体液鉴定方法的特点及其法医学应用价值作一简要综述。     

Human tissue intended for transplantation--FDA. Interim rule; opportunity for public comment

The Food and Drug Administration (FDA) is issuing an interim rule to require certain infectious disease testing, donor screening, and recordkeeping to help prevent the transmission of AIDS and hepatitis through human tissue used in transplantation. The regulations are effective upon publication. FDA is taking this action in response to growing concerns that some human tissue products are being offered for transplantation use without even the minimum donor testing and screening needed to protect recipients against human immunodeficiency virus (HIV) infection and hepatitis infection. The new regulations require all facilities engaged in procurement, processing, storage, or distribution of human tissues intended for transplant to ensure that minimum required infectious disease testing has been performed and that records documenting such testing for each tissue are available for inspection by FDA. The regulations also provide authority for the agency to conduct inspections of such facilities and to detain, recall, or destroy tissue for which appropriate documentation is not available.     

试论人体器官权与器官捐献激励原则(2)(中国政法大学2005届硕士学位论文)

第三章我国器官捐献匮乏的成因分析 一、国内外器官捐献立法回眸 在器官捐献立法方面,外国及我国地方的情况见表1.     

试论人体器官权与器官捐献激励原则(2)(中国政法大学2005届硕士学位论文)

第三章我国器官捐献匮乏的成因分析 一、国内外器官捐献立法回眸 在器官捐献立法方面,外国及我国地方的情况见表1.