A review of the methodological aspects of aspartic acid racemization analysis for use in forensic science.

Accurate age determination of adult cadavers and human remains is a key requirement in forensic practice. The current morphological methods lack accuracy and precision, require specialist training and are costly. The use of aspartic acid racemization (AAR) in human dentine provides a simple, cost-effective solution and the method can achieve accuracies of +/- 3 years at best. Currently, there are differences in AAR methodology between laboratories which produce different results on the rate of racemization in teeth. These inconsistencies must be resolved if the technique is to be successfully applied to age determinations in forensic cases. This paper reviews the differences in protocol which have been used, discusses how each method will affect the results obtained from AAR analysis and gives recommendations for optimization of the methological protocol as a first step towards international standardization.     

A forensic science approach to a starved child

A 19-month-old, 3.6-kg (8-lb) female child dies after a protracted course. The child was premature and suffered a stormy perinatal and postnatal period. When there is underlying disease or a condition potentially sufficient in and of itself to result in a "starved child," isolation of the results of potential neglect presents particular difficulties. The approach to the resolution of this question will be addressed.     

On the added value of forensic science and grand innovation challenges for the forensic community

In this paper the insights and results are presented of a long term and ongoing improvement effort within the Netherlands Forensic Institute (NFI) to establish a valuable innovation programme. From the overall perspective of the role and use of forensic science in the criminal justice system, the concepts of Forensic Information Value Added (FIVA) and Forensic Information Value Efficiency (FIVE) are introduced. From these concepts the key factors determining the added value of forensic investigations are discussed; Evidential Value, Relevance, Quality, Speed and Cost. By unravelling the added value of forensic science and combining this with the future needs and scientific and technological developments, six forensic grand challenges are introduced: i) Molecular Photo-fitting; ii) chemical imaging, profiling and age estimation of finger marks; iii) Advancing Forensic Medicine; iv) Objective Forensic Evaluation; v) the Digital Forensic Service Centre and vi) Real time In-Situ Chemical Identification. Finally, models for forensic innovation are presented that could lead to major international breakthroughs on all these six themes within a five year time span. This could cause a step change in the added value of forensic science and would make forensic investigative methods even more valuable than they already are today.     

The forensic science use of reflective ultraviolet photography

Reflective ultraviolet photography has many forensic science applications particularly in child abuse, rape, homicide, and bite mark cases. The potential of this relatively simple and inexpensive procedure has not been fully explored. The procedure for its use in bite mark cases is presented.     

A risk-based approach to cognitive bias in forensic science

Over the past decade, the potential impact of cognitive bias in forensic science has instigated much discussion and debate between academics, scientists and those in the justice sector. Evidence of bias influencing subjective decision-making across a range of forensic disciplines has been described in the literature. Forensic service organisations are being urged to address cognitive bias in subjective decision-making by designing processes or procedures to limit access to (irrelevant) contextual information or reduce dependence on cognitive functions. Although some laboratories have implemented bias mitigating strategies, with varying impact on operational efficiency, there has been no systematic assessment of the risk posed by cognitive bias. Forensic Science SA assessed the potential impact of bias on forensic interpretations across multiple disciplines, using a risk management framework. This process proved useful in assessing the effectiveness of existing bias mitigating strategies and identified the latent level of risk posed. While all forensic organisations should seek to implement bias limiting measures that are simple, cost-effective and do not adversely impact efficiency, using a risk-based approach has contextualised the limited benefit of introducing resource hungry measures, as postulated in the literature. That is not to suggest that forensic organisations should dismiss the potential influence of cognitive bias but they need to strike an appropriate balance between risk and return, as they do with any business risk.     

Melting curve SNP (McSNP) genotyping: a useful approach for diallelic genotyping in forensic science

The increasing availability of Single Nucleotide Polymorphisms (SNPs) and Deletion/Insertion Polymorphisms (DIPs), as well as the outstanding progress in SNP genotyping technologies, will impact forensics profoundly. We have developed a new method for genotyping SNPs and DIPs, which is based on the determination of melting curve profiles of amplified DNA in solution. We have termed this method Melting curve SNP (McSNP) genotyping. Melting curve profiles are composites of the particular melting temperatures (Tm) of the individual fragments that comprise the DNA sample. Simple mixtures of DNA can be resolved in a very robust and efficient fashion, since the samples can be scored in the plates in which they were amplified with no or very few post-PCR manipulations. As such, McSNP is one of the least expensive genotyping methods available and can and should be useful in forensic science.     

生物超弱发光在法医学中的研究应用展望

生物超弱发光携带着生物体新陈代谢和能量转化的信息，已成为生命学科的重要研究领域。本文回顾生物超弱发光的研究历史、特点及在医学领域的研究应用现状，展望生物超弱发光在法医学．尤其是死亡时间推测和损伤方面的研究应用前景。     

Effective use of forensic science in volume crime investigations: Identifying recurring themes in the literature

New scientific, technological and legal developments, particularly the introduction of national databases for DNA and fingerprints, have led to increased use of forensic science in the investigation of crime. There is an assumption, and in some instances specific assertions, that such developments bring improvements either in broad criminal justice terms or more narrowly in terms of economic or practical efficiencies. The underlying presumption is that the new technological opportunities will be understood and effectively implemented. This research investigates whether such increases in activity have also been accompanied by improvements in the effective use of forensic science. A systematic review of thirty-six reports published (predominantly in England and Wales) since the 1980s, which have considered the use of forensic science in the investigation of volume crimes, was carried out. These reports have identified a number of recurrent themes that influenced how effectively forensic science was used in investigations. The themes identified included forensic knowledge and training of investigators, communication and information exchange between specialists and investigators, timeliness of forensic results, interagency relationships and deployment of crime scene examiner resources. The research findings suggest that these factors continue to hinder the effective use of forensic science despite technological advances and this paper considers their potential causes.     

法庭科学DNASTR分型标准物质初探

本文介绍了标准物质及法医DNA标准物质的概念,并就目前法庭科学DNASTR分型技术所需要的标准物质的制备及应用做一概述,以对法医DNA标准物质的生产提供参考。     

生物超弱发光在法医学中的研究应用展望

生物超弱发光携带着生物体新陈代谢和能量转化的信息,已成为生命学科的重要研究领域。本文回顾生物超弱发光的研究历史、特点及在医学领域的研究应用现状,展望生物超弱发光在法医学,尤其是死亡时间推测和损伤方面的研究应用前景     

Supercritical fluid chromatography in forensic science: a critical appraisal

The application of supercritical fluid chromatography (SFC) in forensic science is reviewed. The applications centre on the analysis of explosives and of drugs of abuse. The systems employed are discussed in the context of comparison with gas chromatography and high-performance liquid chromatography methods which are traditionally used for such analyses. The advantages and disadvantages of SFC over these methods are discussed. Recommendations are made for the developments which are required in SFC technology if it is to find greater application in forensic science.     

New perspectives in the use of ink evidence in forensic science: Part III: Operational applications and evaluation

The research reported in this series of article aimed at (1) automating the search of questioned ink specimens in ink reference collections and (2) at evaluating the strength of ink evidence in a transparent and balanced manner. These aims require that ink samples are analysed in an accurate and reproducible way and that they are compared in an objective and automated way. This latter requirement is due to the large number of comparisons that are necessary in both scenarios. A research programme was designed to (a) develop a standard methodology for analysing ink samples in a reproducible way, (b) comparing automatically and objectively ink samples and (c) evaluate the proposed methodology in forensic contexts.This report focuses on the last of the three stages of the research programme. The calibration and acquisition process and the mathematical comparison algorithms were described in previous papers [C. Neumann, P. Margot, New perspectives in the use of ink evidence in forensic science—Part I: Development of a quality assurance process for forensic ink analysis by HPTLC, Forensic Sci. Int. 185 (2009) 29–37; C. Neumann, P. Margot, New perspectives in the use of ink evidence in forensic science—Part II: Development and testing of mathematical algorithms for the automatic comparison of ink samples analysed by HPTLC, Forensic Sci. Int. 185 (2009) 38–50].In this paper, the benefits and challenges of the proposed concepts are tested in two forensic contexts: (1) ink identification and (2) ink evidential value assessment. The results show that different algorithms are better suited for different tasks. This research shows that it is possible to build digital ink libraries using the most commonly used ink analytical technique, i.e. high-performance thin layer chromatography, despite its reputation of lacking reproducibility. More importantly, it is possible to assign evidential value to ink evidence in a transparent way using a probabilistic model. It is therefore possible to move away from the traditional subjective approach, which is entirely based on experts’ opinion, and which is usually not very informative.While there is room for the improvement, this report demonstrates the significant gains obtained over the traditional subjective approach for the search of ink specimens in ink databases, and the interpretation of their evidential value.     

A cultural change to enable improved decision-making in forensic science: A six phased approach

There has been an increased engagement by researchers in understanding the decision-making processes that occur within forensic science. There is a rapidly growing evidence base underpinning our understanding of decision-making and human factors and this body of work is the foundation for achieving truly improved decision-making in forensic science. Such an endeavour is necessary to minimise the misinterpretation of scientific evidence and maximize the effectiveness of crime reconstruction approaches and their application within the criminal justice system. This paper proposes and outlines a novel six phased approach for how a broadening and deepening knowledge of decision-making in forensic science can be articulated and incorporated into the spheres of research, practice, education, and policy making within forensic science specifically, and the criminal justice system more generally. Phases 1 and 2 set out the importance of systematic examination of the decisions which play a role throughout forensic reconstruction and legal processes. Phase 3 focuses on how these decisions can, and should, be studied to understand the underlying mechanisms and contribute to reducing the occurrence of misleading decisions. Phase 4 highlights the ways in which the results and implications of this research should be communicated to the forensic community and wider criminal justice system. Lastly, the way in which the forensic science domain can move forwards in managing the challenges of human decision-making and create and embed a culture of acceptance and transparency in research, practice and education (learning and training) are presented in phases 5 and 6. A consideration of all 6 connected phases offers a pathway for a holistic approach to improving the transparency and reproducibility of decision making within forensic science.     

Palynology: its position in the field of forensic science

Here we examine the current state of palynology in the field of forensic science. Forensic palynology is discussed with reference to other forensic disciplines to help understand what is required for its progress. Emerging developments are also discussed. Palynomorphs potentially deliver excellent trace evidence, fulfilling the requirements relating to the transfer, persistence, and detection of such evidence. Palynological evidence can provide very powerful investigative and associative evidence. Despite this, the application of palynology to forensic science has had mixed success. There are many anecdotal stories where pollen evidence has had spectacular successes. But it is extremely underutilized in most countries because it is labor-intensive and requires considerable expertise and experience, there is a lack of control over sample collection and inadequate resourcing and funding, and its crime-solving power is not well known. Palynology has been applied to forensic problems in an unstructured way, resulting in a lack of formalized discussion of the underlying principles. As there is renewed questioning of the acceptability of most evidence types in the current legal environment, there is a need for the establishment of palynological evidence through validation-type studies and experimentation, and the implementation of independent proficiency testing.     

Geomatic techniques in forensic science: A review

The purpose of this review paper is to highlight various geomatic techniques that crime scene reconstructionists or forensic practitioners can use to document different kinds of scenes, highlighting the advantages, disadvantages, and when best to use each technology. This paper explores geomatic techniques such as a total station, photogrammetry, laser scanners and structured light scanners and how they can be used to reconstruct crime scenes. The goal of this paper is not to discredit manual methods, as they are long standing and reliable, but instead to shed light on alternative methods that may produce equally or more accurate results with a more visually appealing final product. It is important for law enforcement and forensic professionals to understand the advantages and disadvantages of each technique, knowing when certain techniques should be used (and when they should not), and being able to revert to traditional methods if required.