Two-dimensional metric comparison between dynamic bare and sock-clad footprints for its forensic implications – A pilot study

Footprints may be present at crime scenes as physical evidence. This pilot study compares two-dimensional measurements of bare and sock-clad footprints to determine if significant differences or similarities exist. Dynamic footprints were collected from 30 males and 20 females between the ages of 20 and 61?years old (mean of 28.2?years) using the Identicator Inkless Shoe Print Model LE 25P system. A midgait protocol was employed for obtaining footprints. The fifth and sixth footprint of gait were collected for the right and left foot, respectively, in both sock-clad and barefoot trials. The footprint measurements between sock-clad and bare footprints were compared. The results did not indicate any significant difference (p?>?.05) between bare and sock-clad foot length measurements for right or left feet. Significant differences were seen for the width measurements between bare and sock-clad footprints. These findings have forensic implications, particularly in criminal cases where it is unclear if a footprint impression is from a sock-clad foot or a bare foot. This study shows that such a determination is generally not necessary when utilizing two-dimensional measurements for length comparison between a bare and sock-clad footprint. However, if width measurements are being evaluated, the distinction between bare and sock-clad footprints should be considered.     

Stature and Body Weight Estimation from Various Footprint Measurements Among Egyptian Population

Abstract: Analysis of footprints can reveal very important clues which can be used as a forensic evidence and help in the estimation of stature and body weight of an individual. In this work, bilateral footprints were obtained from 50 male Egyptian medical students ranging in age between 18 and 25. Nine measurements were taken on each footprint. The result revealed significant bilateral asymmetry (p < 0.001) except foot breadth at ball. The significant and positive highest correlation coefficients with stature were shown by toe‐5 length on right side (R = 0.58) and with body weight by foot breadth at ball on left side (R = ?0.52). Regression equations presented smaller standard errors of estimate (3.52–4.69) in determination of stature than those in estimation of body weight (4.05–5.28). In conclusion, this study has provided equations that help to estimate stature and body weight from footprint measurements among Egyptians.     

The contact area of static and dynamic footprints: Forensic implications

When found at crime scenes, footprints may be evidentially valuable and can assist with the identity of a perpetrator based on their features and/or measurements. Footprints can be either static (made while standing) or dynamic (made while walking). While extensive research has been performed on the linear measurements obtained from static and dynamic footprints, research on the comparisons between the contact area of static and dynamic footprints in the forensic context are limited. The present study compares the contact area of static and dynamic bare footprints to determine if statistically significant differences exist between the two. Static and dynamic footprints were obtained from a sample of randomly-selected 461 Jatt Sikh adults (230 males and 231 females) of Indian origin between the ages of 19 and 32 years. The footprint contact area was calculated from each footprint (excluding the toes) using a PedoGRID® sheet. No statistically significant differences were observed between the contact area of static and dynamic footprints for each foot among males and females. However, statistically significant differences between both the sexes were found in the footprint contact areas of both footprint types. The right dynamic footprint contact area was found to be the most predictive measurement for classifying and estimating sex from a footprint’s contact area. The study has implications in the analysis of footprints recovered from crime scenes.     

Reliability of a two-dimensional sock-clad footprint linear measurement method

A two-dimensional linear measurement approach for footprints, herein referred to as the “Reel Method,” has been shown to be valid and reliable when used on bare footprints, however, few measurement approaches have been examined on sock-clad footprints. The present investigation examines the validity and reliability of the Reel Method as a two-dimensional linear measurement approach for use on sock-clad footprints.A walking, mid-gait footprint was collected from 30 volunteer participants using the Identicator Inkless Shoe Print Model LE 25P system. The width of the ball of the foot, calcaneal width, and distance from the most posterior aspect of the heel to the most distal aspect of the first toe were each measured and compared between and within three raters. The statistical analysis demonstrated high reliability of the Reel Method among these three raters. The data were found to be normally distributed, and measurements were consistent between and within raters. 95% Intraclass Correlation coefficients, 95% Bland-Altman Limits of Agreement and 95% Standard Error of Measurement determined high statistical agreement between and within raters, demonstrating that the Reel Method is reliable and thus valid for the linear measurement of sock-clad footprints.     

Two-dimensional metric comparisons between dynamic bare footprints and insole foot impressions-forensic implications

Footwear may be found at crime scenes as physical evidence. Such footwear often has impression features of the wearer’s foot on the insole of the shoe. Scientific research and literature have established that footprints are distinct. This study compares two-dimensional measurements on bare footprints to foot impressions on insoles to determine if significant differences or similarities exist. Dynamic footprints were collected from 51 donors using the Identicator® Inkless Shoe Print Model LE 25P system. Seven foot length and width measurements were taken based on the Reel linear measurement method. Footprint measurements between bare footprints and foot impressions on the insoles were compared. Only two differences (p > 0.05) were observed between the various bare footprint and insole foot impression measurements on the right and left side for most of the measurements, CALC (p < 0.001) and A1 (p = 0.04). Bare footprint and insole A5 measurements on the left side were also significantly different (p = 0.015). The results of the study have implications in the forensic analysis of foot impression evidence on insoles in footwear in assisting with identifying the wearer of said footwear. Situations may arise in the forensic context when comparing the foot impression on the insole of footwear to a suspect’s bare footprint or a footprint from post-mortem remains. This study contributes to the scant literature available on the topic and to understanding the similarities and differences observed in the various linear measurements that may be utilized in the comparison process of footprint impressions on shoe insoles to bare footprints.     

Estimation of stature from footprint and foot outline dimensions in Gujjars of North India

Estimation of individual's stature is an important parameter in forensic examinations. Examination of footprints provides important evidence in a crime scene investigation and helps in estimation of stature of a criminal. Analysis of bare footprints is often carried out in developing countries like India where the footprints are frequently recovered at the scene of crime. The present study attempts to reconstruct stature in a sample of 2080 bilateral footprints and foot outlines collected from 1040 adult male Gujjars of North India ranging in age from 18 to 30 years. Bilateral footprints and foot outlines of each individual were measured for ten and eight measurements, respectively. The results indicate that T-2 length (length of the footprint from heel to 2nd toe) and T-5 length in footprint and T-1 length, T-4 length and breadth at ball in foot outline show statistically significant bilateral asymmetry. Significant and positive correlation coefficients exist between stature and various measurements of footprint and foot outline (P<0.001 and 0.01) except toe 1-5 angle of declination which shows insignificant correlation coefficient. The highest correlation coefficients were shown by the toe length measurements (0.82-0.87) indicating a close relationship between the stature and these measurements. Regression analysis presents smaller mean errors (2.12-3.92cm) in estimation of stature than those of division factor method (3.29-4.66cm), thus, gives better reliability of estimate than the latter. The regression equations were also checked for their accuracy by comparing the actual stature with estimated stature.     

Two-dimensional linear analysis of dynamic bare footprints: A comparison of measurement techniques

In forensic intelligence-gathering, footprints have been shown to be valued evidence found at crime scenes. Forensic podiatrists and footprint examiners use a variety of techniques for measuring footprints for comparison of the crime scene evidence with the exemplar footprints. This study examines three different techniques of obtaining two-dimensional linear measurement data of dynamic bare footprints. Dynamic bare footprints were gathered from 50 students from a podiatric medical school using the Identicator® Inkless Shoe Print Model LE 25P system. After obtaining 100 bilateral footprints from the participants, the quantitative measurement data were collected by using three different measurement techniques: (i) a manual technique using a ruler (direct technique); (ii) an Adobe® Photoshop® technique; and (iii) a GIMP (GNU Image Manipulation Program) technique. The seven Reel linear measurement methodology was used for producing measurements using these three techniques.This study showed that all the mean bare footprint measurements on the right and left feet obtained using the direct technique were larger than those obtained using GIMP and Adobe® Photoshop® images. Differences were also observed in measurements produced using GIMP software and Photoshop images. However, the differences observed in the three techniques used for bare footprint measurements were not found to be statistically significant. The study concludes that there are no significant differences between the three measurement techniques when applied to two-dimensional bare footprints using the Reel method. It further concluded that any of these measurement techniques can be used when employing the Reel methodology for footprint analysis without significant difference.     

Age-related factors in the relationship between foot measurements and living stature and body weight

The measurements of feet and footprints are especially important in forensic identification, as they have been used to predict the body height and weight of victims or suspects. It can be observed that the subjects of forensic-oriented studies are generally young adults. That is to say, researchers rarely take into consideration the body's proportional changes with age. Hence, the aim of this study is to generate equations which take age and sex into consideration, when stature and body weight are estimated from foot and footprints dimensions. With this aim in mind, we measured the stature, body weight, foot length and breadth, heel breadth, footprint length and breadth, and footprint heel breadth of 516 volunteers (253 males and 263 females) aged between 17.6 and 82.9 years using standard measurement techniques. The sample population was divided randomly into two groups. Group 1, the study group, consisted of 80% of the sample (n = 406); the remaining 20% were assigned to the cross-validation group or Group 2 (n = 110). In the first stage of the study, we produced equations for estimating stature and weight using a stepwise regression technique. Then, their reliability was tested on Group 2 members. Statistical analyses showed that the ratios of foot dimensions to stature and body weight change considerably with age and sex. Consequently, the regression equations which include these variables yielded more reliable results. Our results indicated that age and sex should be taken into consideration when predicting human body height and weight for forensic purposes.     

Estimation of stature from static and dynamic footprints

The ability to estimate accurately from known parameters is a fundamental aspect of science and is evident as an emerging approach in the area of footprints and stature estimation within the field of forensic identification. There are numerous foot dimensions that have been measured in the literature to predict stature with varying degrees of confidence but few studies have tried to link the strength of estimation to anatomical landmarks. Such an approach is utilised in this study which estimates stature from the right footprints of sixty one adult male and female UK participants. Static and dynamic footprints were taken from each volunteer using the 'inkless paper system'. The prints were digitised and twelve length, width and angle measurements were chosen for the analysis. The highest correlations with stature were shown to be the heel to fourth toe print for the static group of footprints (r=0.786, p<0.01), and the heel to fifth toe print in the dynamic footprints (r=0.858, p<0.01). Collinearity statistics suggest the heel to fifth toe print length measurement is independent and not influenced by any other variables in the estimation of stature for the dynamic prints. Linear regression equations for this measurement presented the smallest standard error of estimate (SEE) and highest shared variance (R(2)) of all included variables (SEE 4.16, R(2) 0.74). Our study discusses a potential anatomical explanation as to why the lateral border of the foot and hence the impression it makes upon a hard surface, is a more stable indicator in the estimation of stature. The investigation recommends the use of Calc_A4 and Calc_A5 length measurements when estimating stature from footprint impressions.     

Ghosting phenomenon in static and dynamic footprints in India and the United States

Ghosting is the phenomenon that exists when a footprint has a lighter area around the tip of one or more toes or a shadow-like area at the back of the heel. To date, ghosting has been considered primarily a finding of dynamic (walking) footprints, rather than static (standing) footprints. The prevalence of ghosting in static footprints is unknown, and research on its presence in static and dynamic footprints from the same participant is sparse, as are studies on its occurrence in different geographic populations. This study is among the first to evaluate the occurrence of ghosting in the static and dynamic footprints from a particular individual with participants in two geographic populations.A combination of both inkless and ink footprint collection systems were used to obtain a total of 206 bare footprints from 103 adult participants from the United States and India. The data comprised 103 static and 103 dynamic footprints.Ghosting occurred significantly in static footprints, though less frequently than in dynamic footprints. Ghosting in static footprints was seen most often at the first toe, followed by the third and second respectively. This aspect appeared least at the heel. In dynamic footprints, it occurred most at the first toe, followed by the second and fourth toes, and then the third toe and the heel. The prevalence of ghosting in footprints from the United States and India differed in their locations, notably at the first and second toes in the static footprints and at the heel in the dynamic footprints.     

Opiate concentrations following the ingestion of poppy seed products – evidence for `the poppy seed defence'

The universally accepted 300 ng/ml cut-off limit for opiate assays stated to be mandatory for all drug screening laboratories by the Substance Abuse and Mental Health Services Administration, has been questioned recently due to positive results being obtained following the ingestion of poppy seed containing food products. To establish the plausibility of the `the poppy seed defence' the concentrations of codeine, norcodeine, morphine, normorphine and thebaine (a potential marker for seed ingestion) in several varieties of poppy seeds from different countries were quantified by GC–MS. The country of origin of the seed specimen analysed and the preparation of the seeds prior to their culinary use was found to influence the alkaloid concentration determined. The maximum morphine and codeine concentrations determined in the seeds were found to be 33.2 and 13.7 μg/g seed respectively. In addition, thebaine concentrations were found to vary with each seed sample analysed. Following the consumption of bread rolls (mean 0.76 g seed covering per roll) by four subjects, all urine specimens analysed produced negative results (using the Dade Bebring EMIT II opiate screening assay) with the exception of one subject (body weight 63.0 kg) who consumed two poppy seed rolls. In this subject opiate positive screening results were obtained for up to 6 h post ingestion with maximum urinary morphine and codeine concentrations of 832.0 ng/ml (@ 2–4 h post ingestion) and 47.9 ng/ml (@ 0–2 h post ingestion) respectively being achieved. Following the ingestion of poppy seed cake containing an average of 4.69 g of seed per slice by four individuals, opiate positive screening results were obtained for up to 24 h. In one subject (dose equivalent to 0.07 g poppy seed/kg body weight) maximum urinary morphine and codeine concentrations of 302.1 ng/ml (@ 0–2 h) and 83.8 ng/ml (@ 2–4 h) respectively were recorded. The elimination of thebaine was found to vary widely between individuals, therefore suggesting that its absence from a specimen is not necessarily indicative of opiate abuse. These findings demonstrate that the poppy seed defence could be used as an argument in medico-legal and employment medical cases. Great care should therefore be taken when interpreting the data produced when screening for opiates.     

Using brain MERMER testing to detect knowledge despite efforts to conceal

This experiment examined the accuracy and reliability of the memory and encoding related multifaceted electroencephalographic response (MERMER) technique for detecting information related to events subjects have experienced, despite subjects' efforts to conceal that knowledge. Information obtained through interviews was used to develop stimulus sets consisting of words and phrases presented to subjects visually by computer. Sets were composed of three types of stimuli: life experience-related (Probes), stimuli the subject was asked to memorize and respond to (Targets), and irrelevant information (Irrelevants). Each set of stimuli was tested on two individuals: (1) one individual who had participated in the event in question--and thus had the relevant information stored in his/her brain, and (2) one who had not. Six subjects were tested. Electrical brain responses to the stimuli were recorded non-invasively from the scalp and analyzed. MERMERs, (memory and encoding related multifaceted electroencephalographic responses), of which the P300 is a sub-component, were used to determine whether the subject had the relevant information stored in his brain (information present) or not (information absent), thus indicating whether or not each subject had participated in the real-life event in question. Bootstrapping was used to analyze and compare the responses to the three types of stimuli. As predicted, MERMERs were elicited by Probe stimuli only in the subjects who had participated in the investigated event, by Target stimuli in all subjects, and in no case by Irrelevant stimuli. For each of the six subjects, brain MERMER testing correctly determined whether the subject had participated in and consequently knew about the event in question (information present) or had not participated (information absent). The statistical confidence for this determination was 99.9% in five cases and 90.0% in one case. The article concludes with a discussion of areas of future research and the potential for using this new technology as an investigative tool in criminal cases.     

An unusual case of thermal injuries with a hot glue gun: Deliberate self-harm or maltreatment?

Assessing injuries in forensic medicine casework, examiners are often confronted with the question of self-infliction versus third parties' influence, respectively, deliberate self-harm versus maltreatment. We report the case of a 40-year-old male who presented with numerous partially healed thermal injuries of different age. These burns were shaped like capital letters and little circles, which were arranged in lines in a regular form. The lesions were found on the whole body with exclusion of face, genitals, hands and feet. Furthermore, four bitemarks at the right shoulder were noted. Investigations revealed that the man had been abused by his 25-year-old wife (presumably a borderline personality disorder patient) for at least 1 year. In addition to another series of abuses, the woman may have inflicted the shaped burns with a hot glue gun as punishment for breaking certain "rules" she had established. When assessing injuries of patients in forensic medicine, several considerations regarding etiology have to be taken into account. In principle, the victim's testimony, the anamnesis, the police investigation results and the findings from the forensic physical examination have to be balanced against each other. The injury pattern in the present case showed contradictory single characteristics both of deliberate self-harm and of maltreatment. After forensic analysis, it was assessed as injuries inflicted by an assistant with the patient's consent.     

Application of non-contact scanning to forensic podiatry: A feasibility study

Foot impression evidence recovered from crime scenes can be available in the form of barefoot prints, sock-clad footprints, or as impressions within footwear. In some cases, suspects leave their footwear at the scene of the crime, and the insoles from the footwear can be important in linking a person to the footwear. The application of 3D data-collecting technology is becoming more and more popular within forensic science and has been used to recover footwear impression evidence. The present study is a feasibility study to discover if 3D data capturing devices can be applied to insoles; to capture the footprint impression for measurement using the Gunn method (a method used in forensic podiatry casework). Three different methods of data capture were conducted; Adobe Photoshop, MeshLab, and calipers used directly on the insole. Paired t-tests and Intraclass Correlation Coefficient (ICC) were conducted for all three data capture methods. Seven measurements used in this study were significantly different across all three methods. ICC scores were moderate to excellent for the Photoshop method, poor to good for the 3D method, and moderate to excellent for the Direct method.     

The effect of weight on the femur: a cross-sectional analysis

This study assessed whether obesity significantly affects femoral shape. Femora of 121 white men were divided into two weight classes based on body mass index (BMI) of the deceased. Five external anteroposterior (AP) and mediolateral (ML) measurements were taken at consistent percentages of diaphyseal length. These were then subject to statistical tests. After controlling for age, multivariate statistics show a significant (p<0.05) effect of BMI on the femur, with the greatest significance in ML measurements. T-tests confirm these dimensions are significantly larger in the overweight (p<0.05). The effect of BMI on size-transformed and shape-transformed variables was also evaluated, with ANOVA results showing a significant BMI effect on ML size (p<0.05), but not shape. Significant size-transformed ML variables were then subject to discriminate function analyses with a cross-validation correction. Results show a correct classification rate of 88% in normal weight and 77% in overweight individuals.     

Position Effect on Facial Soft Tissue Depths: A Sonographic Investigation

The head is positioned erect for an approximation; yet most facial soft tissue depths (FSTD) used are measured from supine subjects. Depth difference might be significant, but there is a paucity of data to verify. This study compared erect and supine values for 17 landmarks from 30 healthy New Zealand (European population affinity) women (18–30 or 40–55 years) in erect then supine positions. Height, weight, and sonographic FSTD data, totaling 1020 measurements, were obtained. Three midline and seven averaged bilateral values were compared using ANOVA, p values, and Pearson's correlations. Correlative strength of age and body mass index, BMI (kg/m²), was determined by values. Results showed averaged erect and supine differences were significant for four of ten FSTDs. Between individuals, difference was various and not unidirectional. In conclusion, depth differences were observed but not all significant or unidirectional, BMI significantly influenced nine FSTD values, but age group did not.     

Sex determination from hand and foot dimensions in a North Indian population

Hands and feet are often recovered from the site of natural as well as man-made disasters because of bomb blasts, train accidents, plane crashes, or mass homicides. This study is intended to establish standards for determination of sex from the dimensions of hands and feet in a North Indian population. The data for this study comprise 123 men and 123 women aged between 17 and 20 years from the "Rajput" population of Himachal Pradesh in North India. Four anthropometric measurements viz. hand length, hand breadth, foot length, and foot breadth have been taken on both sides of each subject following international anthropometric standards. The hand index (hand breadth/hand length × 100) and the foot index (foot breadth/foot length × 100) were calculated. Sectioning points and regression models are derived for the hand and foot dimensions and the derived indices. The hand and foot dimensions show a higher accuracy in sex determination by sectioning point analysis when compared to hand and foot index. Of the hand and the foot dimensions, hand breadth and foot breadth showed better accuracy in sex determination. Hand index and foot index remain poor sex discriminators in the study.     

Targeting relevant sampling areas for human biological traces: Where to sample displaced bodies for offender DNA?

Sampling strategy is one of the deciding factors in DNA typing success rates. Small amounts of bodily fluid traces and (skin) contact traces are currently not visualized in standard forensic practice. Trace recovery is usually based on the information available in a particular case and on the experience and ‘forensic common sense’ applied by the trace recovery expert. Interactions between an offender and a victim may have characteristic features, resulting in specific trace patterns. Understanding these interactions, and their resulting trace patterns, might improve crime related trace recovery as well as DNA typing success rates.In this study, we examined the interactions between offender and victim when a body has been relocated from one position/location to another. The contact between the hands of the offender and the body of the victim was visualized using a fluorescent dye in a lotion that was applied to the hands of the individual undertaking the relocation. The contact locations were scored and patterns were analyzed based on both victim and offender characteristics (height, weight, age, gender). The resulting patterns were compared to current trace recovery practices in the Netherlands. The results of this large-scale study facilitate evidence-based sampling supporting both investigative and evaluative forensic examinations.     

人和动物SON基因3’非编码区的PCR测序分析

目的 研究人和猕猴、阿拉伯狒狒、猪、牛、羊、马、驴、骡、狗、猫、兔、大白鼠、小白鼠、豚鼠等14种哺乳类动物SON基因3’非编码区(3’UTR)的种属特异性碱基序列差异。方法 对人和上述14种哺乳类的SON基因3’非编码区中的部分种属特异性区域进行PCR测序分析。结果 人有2条扩增片段和14种哺乳类动物各有1条扩增片段的碱基序列;人无关个体和同一种属不同个体动物DNA扩增片段的碱基序列相同,人、猕猴、阿拉伯狒狒、猪、牛、羊、马、驴、骡、狗、猫、兔、大白鼠、小白鼠、豚鼠DNA的SON基因3’UTR扩增片段之间存在碱基序列差异。结论 SON基因3’UTR是一个具有良好种属特异性的遗传标记,采用SON基因3’UTR扩增测序技术可以对人和上述14种哺乳类动物进行准确的种属鉴定。