Distribution of dipyridamole in the organism of warm-blooded animals

Characteristics of distribution of dipyridamolum in the organism of warm-blooded animals (rats) after its intragastric introduction were studied. It was established that intact dipyridamolum in great quantity was present in visceras, blood and excrements of poisoned animals. In the greatest quantity dipyridamolum was present in excrements, tissues of stomach, small intestine, lung, spleen and kidney.     

Isolation of lambda-cyhalothrin from biological material

Dioxan is proposed as an isolating agent for isolating lambda-cytalotrin (LC) from biological material. Optimal conditions for isolating LC from cadaveric hepatic tissue with dioxan were determined and the results of the isolation were quantified.     

Characteristics of alphacypermethrin isolation from biological material

Methanol is proposed as an isolating agent for isolation of alphacypermethrin from biological material. Optimal conditions of isolating alphacipermetrin from human cadaveric liver tissue with methanol are determined and quantitative estimation of isolation results is provided.     

Detection of dipiridamol in biological fluids

Acetone is proposed as an isolating agent for dipiridamol isolation from biological fluids. Purification of the isolates was performed with liquid-liquid extraction and colon chromatography with silasorb C-18 sorbent. The technique of dipiridamol detection in the blood and urine is described. The assays results are presented.     

The detection of the presence of azafen in cadaveric material

Possibility of isolating azaphen from the cadaveric material by acidified water and acetonitrile (as extractant) is shown. Isolation rate (75.6%) of azaphen (with 1 mg of preparation added to 25 g of the liver by acetonitrile is significantly higher than in isolation by acidified water (41.7%).     

差异提取试剂盒在混合斑DNA提取中的应用

目的评估差异提取试剂盒对混合斑样本中的精子和上皮细胞DNA分离提取的有效性。方法采用差异提取试剂盒,选择性裂解精细胞和上皮细胞,结合磁珠法分别对人为控制条件下制备的模拟混合样本和案件中的混合斑检材进行精细胞DNA和上皮细胞DNA的分离提取。对所提取的DNA进行定量分析和STR分型。结果该试剂盒能从精子和上皮细胞不同比例的混合斑中提取出高纯度的精细胞和上皮细胞DNA。结论该差异提取试剂盒适用于性侵害案件中混合斑检材的DNA提取。     

Paternity-testing on paraffin-embedded abortion tissue: preparation of fetal cells may be indispensable

In a case of sexual abuse, a paternity test was performed on paraffin embedded abortion material. STR typing was successful only after isolating fetal tissue from the abortion-material and separately extracting DNA from the excised fetal cells. Examination with five STRs led to a paternity index of 332, confirming the abuse that had resulted in pregnancy.     

利用唾液斑DNA上12S rRNA基因片段进行动物种属鉴定

目的通过检测唾液斑DNA确定案件所涉及动物的种属。方法通过提取动物唾液斑DNA,扩增其线粒体DNA上的12S rRNA基因片段,并进行DNA测序,测序结果在GenBank上进行BLAST搜索,再利用DNA MAN软件进行同源性分析。结果从唾液斑中成功地提取到了基因组总DNA,并成功扩增出了用于动物种属鉴定的12SrRNA基因片段。结论所报道的方法能用于动物种属鉴定。     

激光捕获显微切割技术用于分离混合斑中精子细胞

目的 评估激光捕获显微切割(laser capture microdissection.LCM)技术在分离混合斑中少量精子细胞的应用价值.方法 配制不同比例的精液-阴道上皮细胞混合液.分别用差异裂解法和LCM法分离精子细胞,用磁珠法提取精子细胞DNA,并用IdentifilerTM试剂盒进行STR基因型检测.结果 LC...     

The blast injury as a result of underwater explosion

Experiments on pigs were made for determination of safe distance to blast center for a diver using the isolating breathing apparatus IDA-71U and aqualung AVM-5. The animals were placed at the distance 12, 9, 7 and 5 m from the blast center. After the explosion, basic physiological parameters and severity of traumas were monitored. Differences in morphological characteristics of the damage done on the earth and under water were identified. Safe distance was 12 m.     

The fingernails of Mary Sullivan: developing reliable methods for selectively isolating endogenous and exogenous DNA from evidence

The fingernails of Mary Sullivan, the last victim of the Boston Strangler, were examined to determine if any genetic information about the murderer could be obtained. The nails were extremely friable necessitating the development of new techniques for isolating and purifying DNA. DNA yields from nails were optimized by using a NaOH-based preparation technique, which was simple, efficient, and minimized handling. Methods for selectively and thoroughly removing exogenous material on nails were also developed through use of a species-specific PCR assay, wherein mitochondrial DNA from the nail could easily be differentiated from DNA of contaminating cells.     

Sodium monochloroacetate poisoning of greenfinches.

A large number of passerine birds, mainly greenfinches, were found dead or dying in a hedgerow close to a field of onions recently sprayed with sodium monochloroacetate (SMCA). An analytical method is described for isolating monochloroacetic acid from bird tissues, as its potassium salt, by ion exchange chromatography. The ion exchange eluate is evaporated to dryness, acidified, extracted with ether and the ethereal extract methylated with diazomethane. The concentration of the methyl ester of monochloroacetic acid is determined using the Mass Selective Detector in the selected ion mode. Chemical analysis confirmed the exposure of the birds to SMCA. It is calculated that 50 microliter of spray contained the lethal dose of SMCA for a greenfinch.     

PCR-based identification of postmortem microbial contaminants--a preliminary study.

Investigation of postmortem blood can reveal the presence of significant ethanol levels. However, in some instances it cannot easily be determined if the source of ethanol is from ingestion or from postmortem endogenous fermentation by contaminating microbes. Described here is a robust polymerase chain reaction (PCR)-based method for detecting the presence of common ethanol producing microbial contaminants in human blood. A set of DNA primers were designed for use in PCR to amplify and detect the genomic DNA from humans and three test microorganisms Escherichia coli, Proteus vulgaris, and Candida albicans. A rapid and reproducible protocol was developed for isolating genomic DNA from mixed human blood-microorganism samples that yields a suitable template for PCR. The organism-specific primer pairs can detect the presence of the target microorganisms in human blood at concentrations as low as 10 colony forming units/mL. The PCR products readily can be detected after agarose gel electrophoresis. This method provides an additional means of rapidly identifying microbial contaminants in postmortem blood samples.     

Identification of the source of ivory idol by DNA analysis

In this study, we describe a forensic case dealing with the identification of the source of the processed ivory object by DNA analysis. Two pieces of Lord Krishna's idols from a shop were confiscated by an investigating agency of the Indian government and forwarded to us to identify the source of its origin. We succeeded in isolating DNA from both processed ivory idols by using the phenol/chloroform DNA extraction method. The extracted DNA was subjected to PCR amplification using an elephant-specific mitochondrial DNA (mtDNA) D-loop marker. DNA sequence analysis of the amplified fragment of mtDNA D-loop region confirmed that the idols were consistent with Asian elephant with 99% similarity.     

Sexual psychopathy, public policy, and the liberal state

This article addresses several interrelated issues. It strives to situate a class of offenders within the liberal state. The disposition of this class allows an analysis of the tensions in liberal theory. It highlights the ways liberalism attributes individual responsibility for criminal behavior and captures the limits of those attributions. It also reveals liberalism's shift away from models of responsibility toward the social control of deviancy. The disposition of this class points toward the therapeutic mask involved in the social control model. Further, the disposition of this class may well serve as a model for isolating and demonizing other disfavored classes, including political dissenters.     

Trapped in Resistance: Collective Struggle through Welfare Fraud in Israel

This paper offers a qualitative empirical examination of the noncompliance of Israeli female welfare recipients with welfare laws and authorities. The paper demonstrates that their behavior, defined as “welfare fraud” by the law, is a limited form of collective resistance to the Israeli welfare state. Although the acts of welfare fraud that the women in my study engaged in entail a political claim against the state, the relationship between these acts and notions of collectivity is very constricted in form. The women's collectivity is shown to be constrained by the welfare authorities' invasive and pervasive investigation practices and methods. Due to fear of disclosure to the authorities, the women emerged as deliberately isolating themselves from their immediate environment and potential members of their like‐situated collective. This weakens the connection between the women's acts of resistance and their collectivity, and prevents their acts of resistance from driving social change, trapping them in their harsh conditions and existence.     

Big game species identification by deoxyribonucleic acid (DNA) probes.

Species identification is important in many big game forensic science cases but cannot always be accomplished because of the lack of adequate techniques. The authors have developed deoxyribonucleic acid (DNA) probes for elk, deer, and antelope by isolating highly repeated satellite sequences. These DNA probes distinguish among deer, elk, and antelope, although not between different species of deer. Because of the high number of sequence copies per genome, these probes are extremely sensitive, requiring less than 10 ng of total genomic DNA. The developmental protocol for these probes is relatively simple and is applicable to many other species.     

The Segregative Effects of Discourse

This paper will critically assess thesegregative practices that are ever moreprevalent in the contemporary world from theperspective of Lacanian psychoanalysis. Thesegregation which will be in question in thispaper concerns the current institutional modeof apprehending the subject by isolating andclassifying the subject on the basis of its`external characteristics'. However, thesegregative effects of discourse, it will beargued, are not simply the consequence of atransitory political or epistemologicalimbalance; for in fact, and as will be shownwith reference to Lacanian psychoanalysis, thelogic of segregation is inherent in the verystructure of language. Indeed, discourse isstructured by means of an exception to itselfwhich indexes the place of the subject assplit: this split in the subject, as an effectof language, situates segregation as the veryprinciple of language. In a second moment, thesegregative effects of discourse will be shownto be further amplified by the emptying out ofthe function of sovereignty. In the moderndemocracy, it is the void that now `occupies'the place of exception.     

论“马克思主义东方学”的出发点——兼驳关于马克思、恩格斯没有东方社会理论的观点

马克思、恩格斯关于东方社会的理论包括俄国农民陷入了极其贫困、无法忍受的状况,东方专制制度建立在农民生活的孤立性及彼此隔绝状态的基础上,俄国的"农村公社"面临瓦解的危险,不排除俄国走新式道路的可能性,俄国革命即将爆发并且它将改变俄国和世界的面貌等内容.他们的理论为以后理论的产生和发展起到了奠基的作用,在它的基础上才有以后东方社会理论的发展.关于马克思、恩格斯没有东方社会理论的观点是不正确的.     

The Fitness to stand trial Interview Test: how four professions rate videotaped fitness interviews

The results of this study provide some preliminary support for the use of the FIT as a method for providing structure to interviewers. The FIT may be particularly useful as a guide for making initial decisions about fitness. It was suggested that a screening evaluation based on the FIT could be completed by any properly trained individual with some professional background. The more difficult cases can be referred for lengthier evaluations. Of course, further research on the use of the FIT with actual defendants in real assessments will need to occur before such a procedure can be used as a matter of routine. Finally, the FIT promises to be an effective research tool for isolating professional group differences in definitions of fitness and the importance of different aspects of it, from both a legal and a mental health perspective.