口蹄疫病毒受体猪源整联蛋白β6亚基配体结合域单克隆抗体的制备

以纯化的口蹄疫病毒受体猪源整联蛋白β6亚基配体结合域重组蛋白为抗原,免疫雌性BALB/c小鼠,经3次免疫后取其脾细胞与SP2/0骨髓瘤细胞融合,制备口蹄疫病毒受体猪源整联蛋白β6亚基配体结合域单克隆抗体(McAb);采用有限稀释法和间接ELISA克隆和筛选阳性杂交瘤细胞株,并用SDS-PAGE、间接ELISA以及间接免疫荧光法对制备的McAb的特异性进行鉴定。结果显示,成功获得5株能稳定传代并分泌抗β6亚基配体结合域的杂交瘤细胞株,分别命名为C4C7、E7C7、B8C9、C2D8和B7B6,其分泌的McAb为IgG2b(C2D8)和IgG2a(C4C7、E7C7、B8C9、B7B6)亚类。制备的口蹄疫病毒受体猪源整联蛋白β6亚基配体结合域McAb可为深入研究整联蛋白αvβ6在口蹄疫病毒感染过程中的作用奠定基础。     

口蹄疫病毒感染动物组织及其产品传播口蹄疫的风险和控制

对影响口蹄疫病毒存活的因素、传播途径和最小感染量,口蹄疫感染动物不同组织、体液中口蹄疫病毒的带毒量和病毒存活能力,口蹄疫病毒在各类畜产品中的存活能力等相关研究进展进行了综述;对各类畜产品传播口蹄疫的风险进行了分析评估,并提出了风险控制措施和建议.     

食淀粉乳杆菌对猪A群轮状病毒NSP4在小鼠肠组织表达的影响

为探究食淀粉乳杆菌对猪轮状病毒(PRV)非结构蛋白NSP4表达的影响,对3日龄清洁级BALB/c小鼠分别在感染PRV前后灌服食淀粉乳杆菌,应用实时荧光定量PCR检测感染病毒后空肠组织中PRVNSP4的m RNA相对表达量,采用比色法测定空肠组织内钙离子含量变化,并通过ELISA定量检测空肠组织中整联蛋白α2β1、磷脂酶C和Dicer的含量。结果显示,饲喂食淀粉乳杆菌第5天和第7天时,预防组和辅疗组中NSP4的m RNA相对表达量显著低于病毒组(P0.05);除预防组第11天外,感染病毒后各检测时间点预防组和辅疗组的钙离子含量显著低于病毒组(P0.05);饲喂食淀粉乳杆菌第7天、第9天和第11天时,预防组和辅疗组中整联蛋白α2β1的含量显著低于病毒组(P0.05);感染病毒后各检测时间点预防组和辅疗组中磷脂酶C和Dicer的含量均低于病毒组。以上结果表明:在小鼠感染轮状病毒前后灌服食淀粉乳杆菌均能显著抑制PRV NSP4的m RNA表达和整联蛋白α2β1的合成,并降低空肠组织中钙离子,磷脂酶C和Dicer的含量,这为研究益生菌抗轮状病毒机制提供参考。     

口蹄疫病毒亚单位与宿主细胞凋亡的研究进展

通过对口蹄疫病毒和其他小RNA病毒的蛋白质功能研究进行总结,分析了口蹄疫病毒蛋白参与调控宿主细胞凋亡平衡的作用,并对口蹄疫病毒持续感染形成的机制进行了探讨.     

口蹄疫消毒试验——福尔马林烟雾剂对O型口蹄疫病毒的消毒作用

据文献报导,福尔马林对口蹄疫病毒具有良好的杀毒能力。我们在前一报告中报导了福尔马林溶液对A型口蹄疫病毒的消毒作用。本文介绍福尔马林烟雾剂对人工污染于羊皮样品上的O型口蹄疫病毒的消毒效力试验结果。     

口蹄疫病毒持续感染的研究进展

从口蹄疫病毒和其宿主两个方面综合阐述了口蹄疫病毒持续感染形成的原因,旨在为进一步阐明口蹄疫病毒持续感染的机理和降低持续感染对口蹄疫防控存在的威胁提供理论依据。     

猪水疱病

近八年中,在意大利、英国、法国、奥地利、波兰等国家和香港地区,发生一种由肠病毒引起的猪接触性传染病,临床表现为口蹄疫综合症的症状。开始出现时,临床上都诊断为口蹄疫,但血清学和病毒学检查结果,均未证明病料中有口蹄疫病毒。英国佩布来特动物病毒研究所以及其他如意大利、美国的有关实验室查明,这种新病的病原系猪的肠病毒。1973年1月,国际兽疫局和欧洲防治口蹄疫委员会组织召开了有本病国家的代表会议,会议建议称     

口蹄疫病毒结构蛋白的二级结构及其B细胞抗原表位的预测

以口蹄疫病毒OLZ0 2株基因组序列为材料 ,采用Garnier Robson法、Chou Fasman法和Karplus Schulz法预测了其结构蛋白的二级结构 ,用Kyte Doolittle法分析了各结构蛋白的亲水性 ,Emini法预测了各结构蛋白的表面可能性 ,Jameson Wolf法预测了各蛋白的抗原指数 ,综合评价了口蹄疫病毒结构蛋白的B细胞抗原表位。结果表明 ,VP1蛋白含有的B细胞优势抗原表位最多 ,是研制口蹄疫基因工程疫苗的首选免疫原 ,但其他结构蛋白也含有少量的B细胞抗原表位 ,有的甚至有可能成为优势抗原表位     

O型口蹄疫病毒致弱冷变种特性的研究

口蹄疫病毒同神经病毒及其它病毒均相类似,据此,很多研究者认为口蹄疫病毒在组织培养物中长期降低温度继代,其致病性将随之减弱。借助这种方法,成功地获得了口蹄疫病毒致弱变种。     

The Development of EU-Russian Relations

The EU＇s strategy for Russia since the end of the cold war has to a large extent failed, and the direct consequences are the currently deadlocked EU-Russia relationship. There are three reasons for this： the EU and Russia＇s respective strength and status relative to each other have been changing since the beginning of this century; the EU＇s strategy towards Russia has been more fragmenting in recently years; and the American factor. The EU-Russia relations could continue to be in poor shape in the years to come, and it is very likely sometimes even further worsening. But the two sides would refrain from extreme measures and prevent their relations from sliding into real cold war.     

Young American China Watchers＇ Views on China

In this paper, the author has made a thorough study over the new generation of American China Watchers. Most of the pages are devoted to a detailed comparison between the old generation and the young generation of American China Watchers regarding to their background, education, and views over China. At the end of the paper, the author also gives some policy suggestions for both the Chinese and U.S. government based on the findings in this study.     

China＇s Rise vs. International Order Evolution

A heated debate over the rise of China has been going on unabated in the international community for nearly two decades. However, with the elapse of time, the initial divergence of views in the mainstream opinion has gradually diminished and the gap in China policy among nations has likewise narrowed down. Instead, reason and a realistic approach have eventually taken center stare.     

The Balance Between Coercion and Accommodation

1. Introduction
On the morning of April 1st, 2001, a U.S. EP-3 surveillance aircraft was conducting an intelligence flight along the Chinese territorial border in the airspace south-east of China＇s Hainan Island. Two Chinese fighter jets intercepted the spy plane and followed its flight, as is considered ordinary international practice. For reasons unknown, the EP-3 airplane made a sudden maneuver and crashed into one of the Chinese planes. The Chinese plane dropped into the sea, the pilot missing. The U.S. spy plane was also severely damaged. It intruded into Chinese territorial space and landed on Hainan Island without official permission from the Chinese side. After landing, the spy plane and 24 crew on board were detained by the Chinese authorities. This air collision episode was the first of its kind in the China-U.S. relations and an urgent diplomatic crisis ensued.     

The U. S. Policy toward DPRK: Opportunities and Constraints

布什政府上台以来,始终未能阻止朝鲜拥核步伐.朝核试后,美对朝政策有了新的调整,六方会谈得以复会.但美朝双方受各自国家利益和政策目标的限制,在朝鲜半岛无核化问题上的尖锐分歧难以解决,朝核危机仍存在再度升级的可能.     

Marxist Philosophy vs. International Relations Research in China

This article argues for the confirmation of Marxist philosophy as the ideology that guides international relations （IR） studies in China. Justification comes through the following points. According to Marxism, correct theoretical guidance serves as the prerequisite for correct practice. Marxist philosophy embodies, by nature, righteousness, in opposition to political hegemony. Accordingly, practice under its guidance represents fruitful revolutionary realism. Finally, advanced studies of international relations in this country cannot be separated from philosophical thinking. Using these arguments as background, this article specifically advocates materialism, patriotism, and service to the people as the corner-stone of China＇s IR theory building.     

Excavating Concealed Tradeoffs: How Russians Watch the News

Citizens in democracies are expected to make better decisions if they understand policy tradeoffs. However, politicians rarely have incentives to communicate them; citizens are uncomfortable choosing among valued outcomes; and devising a common metric is difficult. It is not surprising that in the United States the environment provides relatively little cuing or priming of tradeoffs in television news. Russian citizens, on the other hand, face a media environment in which tradeoff cuing is intentionally suppressed by owners' agendas, yet viewers detect concealed tradeoffs even in the absence of tradeoff priming and viewpoint diversity. Analysis of discourse among ordinary Russians in 16 focus groups convened in four cities, differentiated by political reform and media market environments, showed that when watching news in which tradeoffs are thoroughly concealed, viewers challenge stories by offering a broad spectrum of uncued tradeoffs. Tradeoffs come from diverse policy domains and represent a range of cognitive strategies, some of which are considerably more abstract than others and link elements of their observations and assumptions (together with what they can extract from the stories) into complex reasoning outcomes.     

The U.S. Global Strategy as Seen from the Debate in the Primary Election

There may be more than one year to go before the 2008 U.S. presidential election, but the primary election of the two parties has already begun ahead of time. Both the Democratic and Republican candidates for the     

中国需要构建怎样的周边(英文)

正The new central leadership has highlighted the strategic importance of better relations with neighboring countries and the construction of a solid foundation for China’s neighborhood strategy so as to create favorable conditions for Chinese development.1China is strengthening top-level strategy in foreign relations,focusing on global-level relations and staying committed to relations     

中巴关系的发展及其认识误区（英文）

China and Brazil established a strategic partnership in 1993. The bilateral ties have seen rapid development in almost all areas. However, there are eight myths surrounding the China-Brazil relations. These myths are the result of a lack of mutual understanding as well as the misconceptions result misconceptions of the western observers and media. These misunderstandings have of have jeopardized images of the China-Brazil relations and also hindered further development of their bilateral relationship.