扫描电镜/能谱法对92式手枪射击残留物检出率与遗留时间相关性的研究

目的研究92式手枪射击残留物检出率与遗留时间的相关性,为射击时间的推断提供依据。方法实验人员使用92式手枪分别射击1枪和3枪,射击后分别在0.5h内,24h,48h,72h,120h,168h,对实验人员的手部及衣袖袖口处进行射击残留物的提取,利用扫描电镜和能谱仪对射击残留物进行检验。结果射击人的手部和衣袖会附着大量的射击残留物,随着时间的推移,手部的残留物数量急剧减少,24h（射击1枪）、72h（射击3枪）后不能检测到枪击残留物。而衣袖处120h（射击1枪）、168h（射击3枪）内均可检测到射击残留物,且射击残留物的检出率与遗留时间呈负相关性。结论随着遗留时间的增加,衣袖处射击残留物检出率递减,对涉枪案件中射击时间的判断有一定的参考价值。     

92式手枪射击距离与射击残留物分布密度相关性的研究

目的研究92式手枪射击距离与射击残留物分布密度的相关性,为射击距离的推断提供依据。方法选择92式手枪,在带皮的猪肉上覆盖白色棉布,设计了0m,0．1m,0．2m,0．5m,1．0m,1．5m,3．0m,5．0m,10m,15．0m,20．0m,25．0m共12个距离进行射击,提取白棉布上的弹孔圆周和距弹孔2cm的圆周上的射击残留物,利用扫描电镜／能谱仪对不同射击距离遗留在白棉布上的射击残留物进行检验。结果白棉布上射击残留物分布密度与射击距离呈负相关的关系。结论根据棉布上射击残留物的分布密度,能够推断射击距离,对涉枪案件中射击距离的判断有一定的参考价值。     

利用“77”式、“64”式手枪射击弹头痕迹判定枪支种类

使用“77”式、“64”式手枪,击发“64”式7.62mm手枪弹,通过对射击弹头痕迹的观测、统计、和研究,确定判断“77”式和“64”式手枪射击枪种的基本方法。     

发射光谱与计算机联机判断手枪近距离射击距离的研究

本文采用发射光谱与计算机联机检验手枪射击残留物中的特征指示元素,利用特征指示元素黑度差与其射击距离的工作曲线判断手枪近距离射击时的射击距离。在大量实验的基础上摸索出了较佳的实验条件,并通过微机建立了数据处理系统,其方法简单,结果准确。     

八五式7.62mm轻型冲锋枪射击弹壳痕迹初探

通过对八五式轻型冲锋枪射击弹壳的初步观察分析,介绍了该枪射击弹壳留痕的位置、形态、大小等特征,并与五四式手枪、七九式轻型冲锋枪弹壳留痕特征进行了比较。     

根据创口射击残留物推断“5.4”式手枪射击距离的研究

目的利用环境控制扫描电镜/能谱仪（SEM/EDX）研究枪弹创射入口周围射击残留物与射击距离的关系,探讨其能否用于射击距离的判断。方法采用＂＂5.4＂＂式手枪分别在5、10、15、20、30、40、50、60、70、80、90、120cm处射击乳猪皮肤,利用SEM/EDX观察创口周围GSR的分布和成分,并建立回归方程。结果射击距离和GSR颗粒数量之间总体表现为直线关系,并建立了相应的回归方程。当射击距离为10~90cm时,用该方程推断射击距离效果较好。结论检测创口周围GSR可望用于判断“5.4”式手枪的射击距离。     

QSZ92式9mm手枪枪弹痕迹检验

1枪弹简介QSZ92式9mm手枪是我国最新研制成的军用手枪(见图1),该枪使用DAP92式9mm普通弹(见图2),也可发射9mm“帕拉贝鲁姆”手枪弹,发射方式为半自动;闭锁机构为枪管回转式闭锁;击发方式为击锤式;拉杆分离式发射机构;可单动或联动发射;除有手动保险针、还有击针保险和跌落保险;具有膛內有弹指示、弹匣余弹显示及空仓挂机。DAP92式9mm普通弹外形尺寸与国外9mm“帕拉贝鲁姆”手枪弹相同,并可与该弹通用。枪弹技术参数见表1。图1QSZ92式9mm手枪;图2DAPQSZ92式9mm手枪弹表1枪弹技术参数QSZ92式9mm手枪9DmAmPQ手SZ枪92弹式口径:9mm膛…     

毛发枪弹损伤的环境扫描电镜研究

目的探讨毛发枪弹损伤微观形态与射击距离之间的关系。方法应用环境扫描电镜/能谱仪对64式手枪在0~200cm范围内射击造成的毛发损伤及附着物进行形态和成分分析。结果0~30cm射击,毛发表面粘附有大量密集分布的球形射击残留物颗粒,鳞片有严重斑纹状损伤和崩裂缺损;30~60cm射击,毛发表面粘附有较多散在分布的球形射击残留物颗粒,鳞片有轻微斑纹状损伤和崩裂缺损;100cm距离射击,毛发表面粘附有少数个别射击残留物颗粒,鳞片无斑纹状损伤;200cm距离射击,毛发表面无射击残留物。毛发枪弹射击断裂形态差异较大,大体分为剪切断裂和牵拉断裂两种类型,与射击距离无明显相关性。结论毛发表面粘附的大量球形射击残留物颗粒及其造成的鳞片斑纹状损伤对于法医学鉴定贴近距离射击((30cm)具有重要的实用价值。     

捷克产“M52“式7.62mm手枪射击弹壳痕迹初探

捷克产“M52”式7.62mm手枪是近几年来我国涉枪案件中出现率较高的一种外国枪支,由于其能使用国产“51”式7.62mm手枪弹,且射击弹壳痕迹与“54”式7.62mm手枪有许多相似点,因而检验中往往难以区别.笔者对近几年来已破获的一些“M52”手枪射击弹壳痕迹进行了实验研究,并与“54”式7.62mm手枪射击弹壳痕迹进行了比较,发现二者存在许多差异点,现报告     

9mm警用转轮手枪橡胶弹射入口的形态特征

目的观察橡胶弹射人口的形态特征,并探讨其法医学意义。方法使用9mm警用转轮手枪发射橡胶弹,在实验动物胸腹部侧面垂直射击,射击距离分别为紧密接触射击（0cm）,相对接触射击（1～6cm）,近距离射击（6-60cm）和远距离射击（60cm～有效射程25m）。92式手枪采取相同方式射击实验动物作为对照。观察射入口的形态特征,制作组织病理学切片,HE染色显微镜下观察。结果橡胶弹接触射击和近距离射击,射入口边缘整齐,有明显的擦拭轮、挫伤轮和火药斑纹;远距离射击,可见射人口边缘撕裂,挫伤轮明显增宽,皮下出血显著;与手枪弹射入口形态特征明显不同。结论9mm警用转轮手枪橡胶弹射入口典型的形态特征,可作为此类损伤的诊断指标。     

水浸和洗涤血样本的DNA检验

目的对经水作用的血样本DNA分型检验结果进行分析探讨。方法全血样本分为两组,水稀释组用水将全血样本稀释5、10、20、25、30倍后制作血斑;洗涤组分为纯水手洗、肥皂弱洗、肥皂强洗、84消毒液浸洗和洗衣粉机洗等5种洗涤方式。所有样本用IQ试剂盒提取DNA,Identifiler PlusTM试剂盒扩增,并进行分型检测。结果血液稀释组中心部位检材,均无等位基因丢失,除30倍稀释样本外,峰高均衡性均大于70%;外周部位检材出现2～10个等位基因丢失,峰高均衡性均小于50%。洗涤组中除84消毒液洗涤样本未检出DNA谱带外,其余均无等位基因丢失,而峰高及均衡性以手洗和肥皂弱洗样本更好。结论经水稀释或洗涤剂清洗的血样本,即使联苯胺预实验结果为阴性,选取合适的检验部位,仍可获得DNA分型。     

枪击案件中射击残留物提取方法的探讨

目的结合现场勘查提取和实验研究,探讨了几种提取射击残留物的不同方法。方法采用富集浓缩、颗粒收集等方法。结果运用上述方法提取了附着在玻璃、汽车、塑料和织物上弹孔周围的射击残留物。结论收到良好的效果,大大提高了射击残留物提取率和检出率。     

Transfer of fibres on the hands of living subjects and their persistence during hand washing

Textile fibres were transferred to the hands of ten living subjects and their persistence was determined after hand washing. Average number of fibres transferred was 300 ± 133 (female 288 ± 92, male 311 ± 163) per 100 cm² hand area in the 100 experiments. However the number of fibres transferred was not gender dependent but individual dependent. The hand texture of subjects was compared with the number of fibres transferred but the relationship was not observed. The number of fibres transferred varied significantly for the 10 repeated experiments performed under the same conditions for the same subject.The subjects were then asked to wash their hands with water. One test group washed their hands with standing water, and the other with running tap water. Afterwards, the number of fibres remaining on the test subjects' hands were investigated. Migration of the fibres on the surface of the observed hands did occur but total loss of transferred fibre after hand washing did not occur. The average number of fibres remaining per 100 cm² hand area was 14 ± 10 (range = 3–72) for hand washing with standing water, and 10 ± 12 (range = 0–79) for washing with running tap water. The results of this study show the possibility of finding fibres on the hands of a person involved in a criminal case even after hand washing before fibre collection.     

The detection and persistence of Cannabis sativa DNA on skin

The presence of Cannabis sativa DNA was detected on the skin of persons who have recently handled both leaf and resinous material. The persistence of C. sativa DNA was examined on the skin. The subjects were asked to either repeatedly rub their hands on their trousers, place their hands repeatedly into their pockets or wash their hands in soap and water. After rubbing the hands on trousers or placing them in pockets C. sativa DNA could still be detected. No DNA could be detected after washing the hands.     

The propensity of individuals to deposit DNA and secondary transfer of low level DNA from individuals to inert surfaces

We have shown that there is a difference between individuals in their tendency to deposit DNA on an item when it is touched. While a good DNA shedder may leave behind a full DNA profile immediately after hand washing, poor DNA shedders may only do so when their hands have not been washed for a period of 6h. We have also demonstrated that transfer of DNA from one individual (A) to another (B) and subsequently to an object is possible under specific laboratory conditions using the AMPFISTR SGM Plus multiplex at both 28 and 34 PCR cycles. This is a form of secondary transfer. If a 30 min or 1h delay was introduced before contact of individual B with the object then at 34 cycles a mixture of profiles from both individuals was recovered. We have also determined that the quantity and quality of DNA profiles recovered is dependent upon the particular individuals involved in the transfer process. The findings reported here are preliminary and further investigations are underway in order to further add to understanding of the issues of DNA transfer and persistence.     

Suicide,accident? The importance of the scene investigation

We present the as yet unresolved case of the death by gunshot wound of a 21-year-old student from a recent local inspection. It was reported that the daughter of the house had been shot through the window while she was washing the dishes. Slight discrepancies were noted in the statements of the family, who are very religious. The firearm, projectile and cartridge have not been found despite an intensive search. The daughter and the mother tested positive for traces of gunpowder on their hands, while in the case of the son traces were found on his hands and on his vest. That the trajectory of the projectile was from the kitchen outwards was established on the basis of a small hole in the inner pane of the kitchen window and a larger hole in the outer pane. The shot passed through the victim's cheek and the neck. The entrance wound (aditus) on the right cheek had complementary features characteristic of a gunshot from a short-barrelled firearm at relative proximity. The shot passed through the left jugular vein and the left internal carotid artery. The exit wound (exitus) was slightly larger and of irregular shape.The family chose a traditional burial. The mother and son did not present themselves for polygraph testing. A charge was filed against the mother of the deceased. Emphasis was placed on the scene investigation.A covered-up suicide? An accident (a scuffle when trying to prevent suicide)?     

Forensic veterinary radiology: ballistic-radiological 3D computertomographic reconstruction of an illegal lynx shooting in Switzerland

The most popular handgun in Brazil is the single round-barrel caliber 0.38 revolver. In recent years, however, owing to the modernization of police arms and their availability on the legal and illicit markets, pistols have become increasingly popular and currently represent about 20% of police seizures. In a previous paper we presented a novel collection method for gunshot residues (GSR) using a sampling procedure based on ethylenediamine-tetraacetic acid (EDTA) solution as a complexing agent on moistened swabs with subsequent detection using sector field-high resolution-inductively coupled plasma-mass spectrometry (SF-HR-ICP-MS). In the present paper, we discuss the capability of this methodology to identify antimony (Sb), barium (Ba) and lead (Pb) on the hands of volunteers after shot tests with 9 mm and 0.40 in. caliber pistols. Two types of munitions were tested: 9 mm Taurus and clean range. The use of a technique with high sensitivity, such as SF-HR-ICP-MS, permits the identification of low concentrations (less than 1 microg/L) of metals in firearm residue and constitutes a powerful tool in forensic science. We also discuss the importance of the sampling procedure, including collection from a different body part than the gun hand of the suspect. Comparison of the analytical data obtained allows clear discrimination between samples from the hands of shooters and non-shooters.     

Addressing the alternate hypothesis: Transfer and persistence of saliva beneath fingernails

This study investigated the transfer and persistence of salivary DNA under fingernails. This was performed to address a common alternate hypothesis presented to scientists in court, asserting that a relatively large quantity of DNA detected beneath the fingernails, typically from a victim of crime, originates from innocuous transfer of saliva in a casual setting.It was determined through these studies that contact with liquid saliva was an effective way to transfer foreign DNA beneath fingernails. However, when saliva was dried, DNA did not readily transfer through casual contact.When liquid saliva was placed directly beneath fingernails the amount of DNA detected from the saliva donor twenty-four hours later was several hundred-fold lower than the amount detected when sampling occurred immediately following deposition. Furthermore, when the recipients’ hands were washed immediately following the deposition of liquid saliva beneath fingernails, the majority of foreign DNA was removed following one hand washing and all detectable foreign DNA was removed from most recipients’ hands after three or six hand washings.This study demonstrates that casual contact with wet saliva can result in the transfer of substantial quantities of DNA beneath fingernails but that it does not typically persist for extended periods of time and is mostly removed if the hands are washed soon after deposition.     

Capturing Finger and Palm Impressions Using a Hand Cream and Thermochromatic Paper

An inkless set of finger and palm impressions has been obtained on thermochromatic paper by incorporating the protic solvent butylene glycol into a base hand cream mixture, which is rubbed into the hands for 30 sec before donation. This requires no washing of the hands before or after donation, removing many of the difficulties associated with inked impressions. In a random trial of 50 male and 50 female participants (mean age 41 years), 96 found the hand cream pleasant and easy to use and all produced identifiable finger and palm impressions. Following donation, participants continued to rub the hand cream into their hands. Impressions were visible immediately after donation and have not exhibited fading 2 years after being taken. 10% of participants produced impressions that captured third‐level detail, the variation between participants being thought to be associated with the amount of pressure applied during donation.     

Gunshot injury caused by a training bullet]

In the course of a scuffle a 50-year old man has been shot by a revolver. The culprit and witnesses state, there has been only one shot, whereas the victim declared having been shot twice. The medico-legal investigation revealed a gunshot wound that passed through the left thigh, a grazing shot of the right hand and a superficial lesion of the skin at the forehead with sprinkled gunshot residue in the epidermis. The investigation confirmed the statement of the victim: the first shot to the forehead was caused by a plastic training cartridge, the second to the right hand and thigh by a cartridge loaded with an ordinary bullet.