单个细胞DNA样本的制备及其在灵敏度分析中的应用研究

目的建立一种用于高精确灵敏度分析的微量DNA样本制备方法。方法用显微捕获技术制备以细胞为单位的DNA样本,并用于ProfilerPlus试剂盒及ABI310遗传分析仪的灵敏度测试。结果建立了单细胞捕获操作方案,成功制备了依次含1～11个细胞的一组DNA分析样本,并可准确用于ProfilerPlus誖试剂盒及ABI310遗传分析仪的灵敏度研究。结论基于显微操作技术的高精确微量DNA分析样本制备方法可准确用于分析技术方法的灵敏度研究     

PIXE技术在物证鉴定中的应用前景分析

介绍了粒子激发X-射线荧光分析技术及其在物证检验领域的应用,展望了它的前景,为我们进行气态物质分析、对界面的分析、珍贵文物的无损鉴定提出了一条新的思路。     

8个miniSTR基因座复合扩增系统的建立及其应用

目的建立扩增片段〈200bp,包含CSF1PO,TH01,TPOX,D3S1358,FGA,D7S820,D21S11,PentaD 8个miniSTR基因座的复合扩增体系。方法采用四色荧光染料标记引物,PCR扩增后,应用ABI 3130遗传分析仪进行片段长度分析,对280例无关个体,137例疑难生物物证进行了检验。结果280例无关个体的调查结果为除1例在CSF1PO基因座外,其余样本的各基因座分型结果与AmpFLSTR Identifiler试剂盒完全相同。与应用ID试剂盒比较,明显提高了137例疑难生物物证的检出率。结论该检测技术方法稳定,结果准确,重复性好,且其判型结果可进行DNA数据库查询、比对,为刑事案件及灾难事故中疑难生物物证的检验提供了一条新的途径。     

证据规则体系及其中国构建

证据规则经过排列组合可以形成完整的体系。根据其基本作用与实施目标,证据规则体系可分解为关联性规则、辅助性规则和外部政策规则三大类。其中辅助性规则又可细分为优先性规则、分析性规则、预防性规则、简化性规则和定量性规则。我国目前已有一些证据规则的规定,但尚不足以形成完整的体系,影响了我国司法制度的进一步改革和完善。不妨参考借鉴普通法的经验,补自身之不足,以实现证据规则体系整体构建的完成和各个证据规则之间的协调施行。     

随意伪装笔迹及其检验

随意伪装笔迹是故意伪装笔迹的一种，具有故意伪装笔迹的共性特征，但由于它是书写人凭自己主观意识，不借助任何特殊手段，不追求某种固定形式，任意改变自己的笔迹。随意伪装笔迹具有伪装意识的不确定性、盲目性和伪装结果的欺骗性的基本特点，决定了该笔迹伪装类型较多。检验时首先要注意区分是否为伪装，然后再进行下一步的检验。     

上海地区D1S80位点基因频率分布及其在亲子鉴定中的应用

目的：将D1S80位点的DNA多态性分析应用于亲子鉴定。方法;PCR、聚丙烯酰胺凝胶电泳及溴已锭染色。结果：获得D1S80位点的DNA多态性分布数据。结论：D1S80位点的PCR检测方法可成功地用于亲权纠纷案的鉴定。     

关于我国刑事悬赏的法律透析及其制度构建——从马加爵案件谈起

刑事悬赏作为刑事侦查中行之有效地破案措施,在国内外的侦查实践中已经得到广泛的应用。要正确运用这一措施,必须从法律角度对刑事悬赏的概念、性质、理论依据、司法功能进行深入的分析,从而设计出符合我国国情的刑事悬赏制度。     

Use of proteases to improve the sensitivity of ABO antigen detection by absorption-elution test in analysis of human excretion traces

The highest sensitivity of the absorption-elution test for detection of A, B, and H antigens in human excretion traces was observed when the excretion traces were treated with protease C and lidase (hyaluronidase) and standard erythrocytes for analysis of the resultant eluates were treated with protease C. Cross-reactions of sera with antigens of contralateral specificity were ruled out by simple dilution of the sera; the reliability of antigen detection was not lost due to pronounced increase in the sensitivity of modified absorption-elution test.     

加强党的执政能力建设,自觉经受长期执政的考验

本文结合对党的十六届四中全会《中共中央关于加强党的执政能力建设的决定》的学习,对以江泽民同志为核心的第三代领导集体提出的最新课题加以解读,从“执政能力建设”科学命题提出的背景、科学内涵和“执政能力建设”的方法和途径等角度做出了有益探讨。     

Evaluating the sensitivity,stability, and cross-reactivity of commercial fentanyl immunoassay test strips

Illicit fentanyl has flooded the United States' drug market, increasing the risk of overdose and poisonings throughout the general population and accidental exposure among law enforcement officers confiscating the increasing number of seizures. Fentanyl test strips (FTS) are used to obtain presumptive information about the presence of fentanyl in a suspected sample. However, their adoption by law enforcement personnel and seized-drug analysts has been limited because most products are advertised for urine testing, not for assays using water solutions. This study presents an evaluation of four commercial FTS: Rapid Response from BTNX, Inc.; T-Dip Fentanyl (FTY) Urine Dip Cards obtained from Amazon.com ; Premier BioDip FYL10 from Premier Biotech Inc.; and MobileDetect Fentanyl strips from DetectaChem, Inc. Performance characteristics curves were used to compare the products' sensitivity, showing that all can reliably detect fentanyl in aqueous solutions at concentrations below 1 μg/mL, with some of the tests able to reliably detect the drug at 200 ng/mL. A stability study demonstrates the performance of all four FTS brands was only slightly affected after 30 days of storage at two extreme environmental conditions. Fentanyl-related substances are also evaluated using the Rapid Response FTS, which showed high cross-reactivity with para-fluorofentanyl and acetylfentanyl, but lower with ortho-chlorofentanyl, carfentanil, and 4-ANPP. Users should be aware that FTS may give false-negative results even when potentially dangerous levels of carfentanil are present. When testing other common drugs, adulterants, and diluents frequently encountered in seized tablets, concentration-dependent results were obtained and multiple instances of false positives were recorded.     

浅论公安科技与标准化

本文论述了公安标准化在公安科技工作中的作用及意义,分析了公安科技的发展和公安标准化之间的关系。本文对于强化公安科技工作的科学、规范管理,充分发挥公安标准的作用具有一定的应用意义。     

全基因组扩增技术的法医学应用价值及其结果评价

微量模板DNA的分析,一直是法医学特别关注和急需解决的难题之一,近年发展起来的一系列技术为解决这一问题提供了新的可能途径,本文即对微量模板DNA分析技术的进展和目前逐步发展的全基因组扩增技术的法医学应用价值、结果评价进行浅议。     

数字图像复原技术的原理及在刑事技术中的应用

目的介绍数字图像处理技术在刑事案件复原的应用,促进该技术的发展。方法利用数字算法、滤波、几何运算等计算机图像处理技术进行复原处理。结果对案件的隐含、残缺、微弱信息,模糊图像、变形物证复原及特征显现。结论刑事数字图像复原技术在刑事技术领域有了广阔的应用前景。     

多源图像融合技术及其在公安刑事摄影工作中的应用

近年来,多源图像融合技术的应用越来越多,它可以将同一场景以不同成像方式或者不同成像时间获得的不同图像融合为一幅图像。这种技术可以综合多张照片的信息量,提高视觉感知,便于进行计算机的处理,因此,可在公安刑事摄影工作中用于对疑难指纹、立体痕迹、强反光客体痕迹、高逆光现场照片等的拍摄和处理,从而最大程度的准确反映被摄景物的实际情况。     

DNA鉴定技术及其在刑事侦查中的应用

DNA鉴定技术自产生以来,在国内外有了一定的发展。就其在刑事侦查中的价值而言,它能够起到锁定犯罪嫌疑人、进行尸源认定、为串并案件提供依据、排除犯罪嫌疑等作用。DNA鉴定技术并非绝对可靠,尚有一些问题值得我们注意。     

Differential association theory reconsidered: An extension and its empirical test

In 1974 the German methodologist Karl-Dieter Opp expounded and expanded Sutherland's differential association theory. In this article an empirical test of this version of the theory is presented based on data for 1196 boys and girls in the age range 12 to 17 years. Furthermore, some new and additional theoretical specifications about the social influence of others on the individual, all in accordance with the original ideas of Sutherland, are proposed and empirically tested. The differential association theory according to the version of K.-D. Opp is fairly well corroborated by the data. Only three of the postulated relationships are rejected. The theory explains 51% of the variance of criminal behavior, even considering that no criminal population is used for the test and only minor offenses are measured. The test also shows that the impact of the frequency of contacts with deviant behavior patterns on the development of positive definitions and on the frequency of communication about relevant techniques is substantial and cannot be ignored by criminologists. Furthermore, special analyses show that several propositions favor the theory. It is the deviancy of others that has the most substantial impact: the more youngsters have contact with their friends, the stronger the impact of the deviancy of their friends on the development of positive definitions or on the frequency of communication about techniques. The tests also show that the more youngsters identify themselves with others, the stronger will be the impact of the deviancy of the others on their norms. These results support the modification of the DA theory according to Opp and falsify some propositions of social control theory.     

Hair analysis for ketamine and its metabolites

A rapid and sensitive method was developed for the simultaneous identification and quantitation of ketamine (K) and its major metabolite, norketamine (NK) in hair. After decontamination, the hair sample was incubated and extracted, and analyzed by gas chromatography-mass spectrometry (GC-MS). Limits of quantitation were found to be 0.05 ng/mg. Hair segments in black color were collected from 15 K abusers. Based on an experiment with 15 cavies with black, white, and brown hair, the mechanism of incorporation of K into hair was investigated. After shaving hair on the back of the cavies (8 cm x 4 cm), they were separated into three groups and administered intraperitoneally once a day for 7 successive days with high, medium, and low doses of K, respectively. Two days after this, hair segments with different colors were shaved. There was a direct correlation between the concentration of K in cavy hair and the dose and DHNK was detected only in high dosage group. The concentration of K increased in the order of white, brown, and black hair. The possible factors responsible for the incorporation of K and its metabolites in hair were discussed.     

The safety helmet detection technology and its application to the surveillance system

The Automatic Teller Machine (ATM) plays an important role in the modem economy. It provides a fast and convenient way to process transactions between banks and their customers. Unfortunately, it also provides a convenient way for criminals to get illegal money or use stolen ATM cards to extract money from their victims' accounts. For safety reasons, each ATM has a surveillance system to record customer's face information. However, when criminals use an ATM to withdraw money illegally, they usually hide their faces with something (in Taiwan, criminals usually use safety helmets to block their faces) to avoid the surveillance system recording their face information, which decreases the efficiency of the surveillance system. In this paper, we propose a circle/circular arc detection method based upon the modified Hough transform, and apply it to the detection of safety helmets for the surveillance system of ATMs. Since the safety helmet location will be within the set of the obtainable circles/circular arcs (if any exist), we use geometric features to verify if any safety helmet exists in the set. The proposed method can be used to help the surveillance systems record a customer's face information more precisely. If customers wear safety helmets to block their faces, the system can send a message to remind them to take off their helmets. Besides this, the method can be applied to the surveillance systems of banks by providing an early warning safeguard when any "customer" or "intruder" uses a safety helmet to avoid his/her face information from being recorded by the surveillance system. This will make the surveillance system more useful. Real images are used to analyze the performance of the proposed method.