Gas chromatographic identification and quantification of hydroxyzine: application in a fatal self-poisoning

A method for the identification and quantification of hydroxyzine in human fluids by GC/NPD is presented. The method employs acepromazine as the internal standard and requires no derivatization. After a single alkaline extraction in n-heptane isoamylalcohol (98.5:1.5, v/v), analysis is achieved in 7 min. The lower limit of detectability was 0.8 ng/ml in plasma. Capillary gas chromatography mass spectrometry assay was developed for confirmation. Toxicological findings, after a fatality involving hydroxyzine are presented as an application of the procedure.     

Study of ethno-racial and sexual specificity of hard palate mucosa for person identification

Relief of hard palatal mucosa was examined to identify ethno-racial and gender affiliation in representatives of europeoid, mongolid, and intermediate racial groups, viz Estonians (northern europeoid type), Azerbajaninans (southern europeoid), Koreans (Far East mongoloid), and Kasakhs (intermediate). The results of the study suggest the possibility in principle to identify the ethno-racial affiliation of human subjects from the distribution pattern of hard palate mucosal folds.     

The specificity of electron impact mass spectroscopy for the identification of N-ethyl-1-phenylcyclohexylamine (PCE)

The electron impact mass spectrum of N-ethyl-1-phenylcyclohexylamine (PCE) was studied using both deuterium-labeled compounds and structurally related analogs. The deuterium-labeled compounds used were d2 . PCE with two deuterium atoms on the methylene carbon of the N-ethyl group, d3 . PCE with three deuterium atoms on the methyl carbon of the N-ethyl group, d4 . PCE with four deuterium atoms on the beta carbons of the cyclohexyl rings, and d5 . PCE with five deuterium atoms on the phenyl ring. Structurally related compounds used included the N,N-dimethyl, N-propyl, and cyclopentyl analogs. The identities of some major fragments and possible pathways leading to their formation are shown. Electron impact mass spectroscopy is shown to be a definitive test for the identification of PCE.     

A simple gas chromatographic identification and determination of 11 CNS stimulants in biological samples. Application on a fatality involving phendimetrazine

A method is presented for the simultaneous identification and quantification of several CNS stimulants, including amphetamine in plasma and urine by GC/FID using mephentermine as an internal standard. No derivation is necessary and after a single alkaline extraction, GC analysis for the 11 compounds tested is achieved in 23 min. The lower limit of detectability was found to be 4 ng/ml for amphetamine in plasma. This method is sensitive, reproducible, selective and applicable in forensic and clinical toxicological analyses. Toxicological findings, after a fatality involving phendimetrazine are presented as an application of the procedure.     

Detection of delta 9-THC in saliva by capillary GC/ECD after marihuana smoking

A method is described for the determination of delta 9-tetrahydrocannabinol (delta 9-THC) in the saliva by the use of a combination of moving-precolumn injector and glass capillary gas chromatograph with electron capture detector (GC/ECD). There were no interfering peaks due to impurities around the peak of pentafluoropropyl derivative of delta 9-THC (delta 9-THC-PFP). This GC/ECD method was linear over the range of 5-200 ng/ml of delta 9-THC-PFP. The lower detection limit was approximately 1 ng/ml. delta 9-THC content in the saliva after experimental marihuana smoking was measured by this method. It was demonstrated that for at least 4 h after smoking the level of delta 9-THC was sufficient for detection.     

Enzyme immunochemical detection of the use of hashish and confirmation by thin-layer chromatography

The EMIT cannabinoid assay was used for screening blood and urine after smoking tetrahydrocannabinol (THC; 10 mg) or ingestion of THC (30 mg). Cannabinoid levels in urine remain detectable up to 1 week. Confirmation was done by adsorption of the THC carboxylic acid onto a C18 extraction column and elution with acetone and TLC. The method is simple and sensitive and is applicable with common laboratory equipment. The detection limit is 10 ng/ml, using 10 ml urine.     

Private roles in food safety provision: the law and economics of private food safety

Private food safety standards play a crucial role in ensuring the safety of the foods we consume. A voluntary instrument, private standards are so widespread to have become de facto mandatory for suppliers who wish to access the most profitable markets. Developed by retailers and business coalitions and enforced through third-party certification, private food safety standards constitute one of the principal food safety governance instruments of agribusiness value chains. Albeit private and voluntary, such standards have profound public implications because they contribute to food safety and protect consumers’ health. This article uses law and economics theory to identify their strengths and vulnerabilities and understand the relationship between public and private regulation. Specifically, it examines whether private standards can fulfill the public interest objective of protecting consumers’ health and whether they compete with or rather complement public regulation. The article argues that private standards have emerged in response to food scares to coordinate complex food value chains and have become ever more relevant in the context of intense market globalization, an area in which public regulation often failed. Among the advantages of private standards, are their flexibility and ability to rapidly respond to new risks. Through their focus on management-based regulation and strong market incentives for producers, private standards promote compliance better than traditional inspection methods. Private standards also present several gray areas including increased risk of capture due to their limited transparency and gaps in enforcement by third-party certifiers. The article suggests areas that deserve additional scrutiny, especially the opacity of standards vis-à-vis consumers and the public sector and the quality and reliability of third party certification.     

Separation of visibly-excited fluorescent components in fingerprint residue by thin-layer chromatography

The use of lasers for the detection of fingermarks is widespread in the forensic field. Despite this, and the fact that many studies have been conducted into the composition of fingermark residue, the components responsible for the inherent visible fluorescence remain unidentified. Traditionally compositional studies have been performed on sweat, sebum, or skin surface washes, none of which are truly representative of the situation when a fingerprint is deposited on a surface. In this paper thin-layer chromatography (TLC) has been performed on sebum-rich fingermarks laid directly onto TLC plates and an argon ion laser used to visualize the separated components. It has been found to be a robust and reproducible method for studying the fluorescent components in fingermark residue and is considered to be more realistic than other methods of sample preparation as it eliminates the chances of extraneous matter being extracted from the skin surface. Investigations into the nature of the separated compounds have also been made and the results are reported.     

Screening of benzo- and thienodiazepines in urine with Emit ST and thin-layer chromatography within the scope of substance abuse

All 24 Benzo- and Thienodiazepines which are used in drugs in the FRG were added to drug free urine in different concentrations and examined by Emit ST as well as by TLC. In addition the acid hydrolysis products of diazepines were analysed by TLC. A modification of the extraction procedure is described, which shortens analysis time considerably. The detection limits were determined and it will be discussed how both methods, Emit and TLC, are suited for a wide-ranging drug screening program.     

Detection of glycosphingolipids of ABH and Le antigens on thin-layer plates using the PAP method

Stroma from hemolyzed erythrocytes of blood groups 0, A1, B and A1B were obtained and subjected to butanol/phosphate buffer extraction. This extract was separated using HPTLC, and the ABH and Le substances were detected on the chromatogram using the PAP technique. The staining of the bands allowed specific demonstration of the serologically active glycosphingolipids present in the ABH and Le blood group substances. The antigens of the AB0 system showed a 3- to 12-band pattern. Each of the antigens Lea and Leb presented 3 bands. The slight differences in the levels of glycosphingolipids of equal chain lengths are probably due to differences in their chemical structures.     

The detection of fetal hemoglobin in bloodstains by means of thin-layer immunoassay

A method for the detection of fetal hemoglobin in bloodstains by means of thin-layer immunoassay is described. The equivalent of 0.01 microL of blood containing 0.18 to 0.24 microgram of fetal hemoglobin may be detected by this method. Studies with stains up to two years old and blind studies have shown these methods to be sufficiently sensitive and specific to be of value in forensic serology.     

Analysis of thin-layer chromatograms of paint pigments and dyes by direct microspectrophotometry

The Nanospec 10S microspectrophotometer has been used to produce reflectance spectra over the 380–900 nm range from coloured thin-layer chromatography spots. The method has been applied to extracted paint pigments and to dyes from other materials examined in forensic science.     

Sequencing of mitochondrial DNA and the problem of human specificity

When analysing trace materials and degraded DNA the issue of human specificity is highly important. Especially when it comes down to the analysis of mitochondrial DNA which is extremely susceptible to contamination authenticity is the main question. Therefore in the presented study mitochondrial primers were tested on their human specificity. In all cases it was possible to amplify DNA of animals with human mt-primers. These unintentional amplifications could only be decreased by choosing austere PCR parameters. The study implies the importance of comprehensive evaluation of primers, chemicals and PCR parameters.     

The specificity of healing of fractures in the skull base

On the basis of actual observations we elucidated the main processes occurring in the reparation of fractures in the skull base. The specificity of reparation was shown to be conditioned by the structure of base bones and of surrounding tissues as well as by the nature of blood supply to them. Criteria are suggested for the determination if the lesions were inflicted in life time and when.     

Illuminating the health and safety of luminol

Luminol is a reagent that is used to enhance areas of non-visible bloodstaining and it is one of the most sensitive of such reagents available to the forensic scientist. However, its use, particularly within the UK and some other European countries, has been limited, predominantly due to concerns about the health and safety of the reagent. This paper reviews the literature currently available regarding the health and safety of luminol, and in the authors' view demonstrates that there are no significant health and safety concerns with the preparation of luminol solution and its application at the crime scene or in the laboratory, providing suitable precautions are taken.     

保安处分适用的瓶颈及其解决

保安处分在控制与预防犯罪方面具有重要价值 ,但是 ,由于保安处分的适用要件———人身危险性难以把握 ,因而 ,保安处分存在适用风险。本文在国内外学者研究的基础上 ,提出将具有可评估性的人格概念引入保安处分理论中 ,从而有效地控制保安处分适用的风险。     

Use of thin-layer chromatography for detection of 11-nor-9-carboxy-delta9-tetrahydrocannabinol in the urine

A simple method of thin-layer chromatography for detection of 11-nor-9-carboxy-delta9-tetrahydrocannabinol in biological material (urine) can be used in chemicotoxicological laboratories and chemical departments of forensic-medical expert examination without special facilities.     

Researching safety and cost-effectiveness in the life cycle of nanomedicine

Nanotechnology is rapidly emerging as a transformational influence on many industry sectors. This is particularly true of medicines and medical devices. This article argues that, as policy interest in devising an appropriate regulatory framework for nanotherapeutics escalates, it will be important for public health to ensure that a broad life-cycle approach to both safety and cost-effectiveness is adopted. It charts some of the most important issues likely to be faced and begins to map how they can best be addressed.