Comparison of DNA yield after long-term storage of Second World War bone samples

Sample storage is of paramount importance in forensic genetics laboratories since only optimal storage enables successful recovery of DNA from old bones that contain very low amount of severely degraded DNA. When identification of missing persons from skeletal remains is completed, bone sample is routinely stored at -20 °C for long-term storage for retesting in future, if necessary. After molecular genetic analyses of Slovenian Second World War (WWII) victims, small fragments of femurs were stored at -20 °C. Reduction in DNA recovery has been observed in frozen liquid DNA extracts by some authors and the goal of our study was to explore how freezing of bone samples affects the preservation of DNA. To achieve this goal, the difference in DNA yield in extracts obtained from WWII bones analyzed in 2009 (data from published paper) and DNA yield in extracts obtained from the same bones (piece sampled next to the one used in 2009) taken out of the freezer after long-term storage on -20 °C for 10 years was examined, using the same extraction method and the same quantification kit. Up to 100 ng DNA/g of bone powder was obtained from 57 WWII femurs and up to 31 ng DNA/g of bone powder from the same femurs investigated after long-term storage in this study. 0,5 g of bone powder was decalcified using full demineralization extraction method. The DNA was purified in a Biorobot EZ1 device (Qiagen) and DNA quantity determined with the Human Quantifiler kit (TFS). Statistical analysis showed significant difference in DNA yield in extracts obtained from WWII bones in 2009 and extracts obtained from the same bones stored at -20 °C after 10 years. As reported for frozen liquid DNA extracts, reduction in recovery of DNA was confirmed for frozen bone samples as well.     

Next generation sequencing technology in Second World War victim identification

The killings during the Second World War (WWII), with nearly 100,000 victims, is one of the greatest losses of life in Slovenia’s modern history and most of the victims are still buried in hidden mass graves and remain unidentified. Identity, ancestry, and phenotypic SNPs, as well as STR markers are already used for solving various cases with Next Generation Sequencing (NGS) technology. In this study, the Precision ID GlobalFiler NGS STR panel was used to identify the WWII victim that could not be identified with capillary electrophoresis (CE) analyses because limited statistical support was obtained after amplification of autosomal STRs using CE STR kits. Bones and teeth were analysed and compared to family references (nephew and niece on paternal line). Prior to DNA isolation 0.5 g of powder was decalcified. The DNA was purified in a Biorobot EZ1 device. The nuclear DNA of the samples was quantified with the PowerQuant kit. Because the recommended posterior probability (PP) of 99.9% was followed with the goal of high confidence of correct identification, the NGS STR Panel was used, and after the analysis of additional STR loci the statistical calculation showed a PP of 99.99986%, showing that a large enough number of genetic markers were analysed when identifying the skeletal remains of the aunt. PP value endorsed the hypothesis that the tooth and bone samples were from individual related to the family references rather than from unrelated individual. In presented case, NGS technology proved to be a powerful tool for increasing the number of autosomal STRs needed for identification of WWII victims when linear markers cannot be used for comparison and only distant relatives are available for analyses.     

Winning the war but losing the peace: The British House of commons during the Second World War

This article considers competing hypotheses ‐ the marginalisation and the constraining hand theses ‐ about the relationship of the House of Commons to government during the Second World War and the consequences of that relationship for the House of Commons in peacetime. The evidence supports the constraining hand hypothesis, with the situation created by war generating a House of Commons with more influence over the executive than is generally conceded. However, the consequence of that experience was to deter the House of Commons from undertaking significant, and arguably necessary, institutional reform in peacetime.     

战后日本的民法修改与家制度

20世纪初以后,随着社会的变革,日本传统的家制度越来越不能适应现实的需要,因此,战前已开始出现缓慢变革的家制度,战后在以美国为主的占领军强制下,通过民法的大幅度修改实现了根本性的变革,但同时,社会的实际变化没有与之同步。     

Storage of Second World War bone samples: Bone fragments versus bone powder

Bone samples may yield low-quality and low-quantity DNA and duplicated analyses of different genetic markers have to be performed for identification of missing persons. Mostly no DNA extract is left after analyses and efficient storage of bones is needed to ensure the stability of the sample over time for retesting using new markers and new technologies. Usually not all of the bone powder prepared in grinder is used for extraction and rest can be stored for future analyses. After molecular genetic analyses of 88 victims of Second World War (WWII) Konfin I mass grave in Slovenia (performed in 2009), fragments of femurs and bone powder that were left were stored at -20 °C. Some authors reported that long-term storage of powder results in the reduction of DNA preservation and its degradation (even at low temperature), explained by an increase in oxidative damage as a result of the enormous increase in exposed surface area. Consequently, grinding of bones as shortly prior to DNA extraction was recommended. The goal of our study was to explore the difference in DNA yield between bone fragment and bone powder frozen for 10 years. 57 WWII femurs were examined and DNA extracted from each of them using bone fragment (piece sampled next to the one used in 2009) and bone powder obtained in 2009, both taken out of freezer after 10 years of storage. Half gram of bone powder was decalcified using full demineralization extraction method. The DNA was purified in a Biorobot EZ1 (Qiagen) and quantified with PowerQuant kit (Promega). Statistical analysis showed significant difference at the 0.05 level in DNA yield comparing fragments of bones and bone powder stored at -20 °C for 10 years. The results show there is more DNA stored in the bone powder than in the bone fragments. Because of time - consuming powdering procedure we recommend to store not only the fragment of the bone, but obtained bone powder as well.     

Settlement of refugee women and children following the Second World War: challenges to the family

Abstract

Following the Second World War, refugees that were displaced as a result of conflict became a global concern. Many of these displaced persons were resettled under the auspices of the International Refugee Organization. Large numbers of European displaced persons settled in Australia, with significant numbers living in Queensland. The wartime and migration experiences of refugees have the potential to influence settlement experiences, and for displaced persons who settled in Queensland, these prior experiences continued to resonate within the family throughout the settlement process. Many refugee children became separated from their families, and while some were reunited, the process was lengthy and not without its difficulties. The effect of separation and loss experienced by these families dominated the post-war experience. It presented challenges to mothers, children and families as they negotiated the uncertainty of displacement and the potential for resettlement, and affected their approaches to separation and reunification. The separation and reunification of children and families played a significant role in the settlement process, influencing interactions with Australian organisations and the development of familial and social network connections during settlement. The legacy of conflict and separation continued to resonate within families and influenced perspectives of the ongoing settlement process for child refugees.     

Different skeletal elements as a source of DNA for genetic identification of Second World War victims

To this day process of identification of missing persons from skeletonized human remains with help of forensic genetics proves to be complex and challenging. The success rate of genetic identification in bones strongly depends on a combination of various factors, most importantly environmental factors and post-mortem interval. Furthermore, there are individual-specific factors that affect DNA preservation, such as race, gender, age and type of skeletal elements. The goal of our study was to optimize sampling process through determining which skeletal elements are superior in their preservation of DNA in 70-yearold skeletons belonging to victims of Second World War. We sampled different types of bones and teeth from three such skeletons found in Slovenian hidden mass grave Huda jama, 56 elements from each respective skeleton, together 168 elements. With the help of parameters, such as quantity of DNA, degradation rate and typing success, we tried to find the best types of elements to identify the victims. Prior to powdering bones and teeth, we removed contaminants. We decalcified 0.5 g bone and tooth powder followed by extraction and purification of DNA using Biorobot EZ1 (Qiagen). Quantification of obtained nuclear DNA was carried out using PowerQuant kit (Promega) and autosomal STR typing using ESSplex SE QS kit (Qiagen). Best parameters to assess skeletal elements that are superior in their DNA preservation were quantity of DNA and number of successfully typed STR loci. Metacarpal and metatarsal bones proved to be the best, followed by intermediate cuneiform, first distal foot phalanx, talus, petrous bone and tibia. We also created elimination database for persons involved in exhumation, anthropological and genetic analyses and exclude potential contamination.     

Dealing with the holocaust and collaboration: The Dutch experience of criminal justice and accountability after World War II

This paper addresses the problem of ``settling accounts' after periods of armed conflict in a given society (be they civil war, insurrection or occupation by foreign forces), when those who took the losing side have come to be defined as collaborators, a process now known as transitional justice. More specifically, it looks at the way in which Dutch citizens who collaborated with the Nazi occupation forces in deporting and murdering about 80% of the Jewish population of the country, were dealt with after the Second World War. There are generally assumed to be three ways of coming to terms with such traumatic events: prosecution and criminal trial, truth and reconciliation commissions or a combination of both. Under present international law, states have a duty to use the criminal law and to prosecute and punish perpetrators of crimes againt humanity and war crimes, specifically because it is felt that prosecution will bring some measure of recognition and healing to victims. After the Second World War in the Netherlands, the emphasis was indeed on criminal law and the manifest aim was swift and just retribution. The author shows how this was frustrated by political considerations; but – and perhaps this is a more important lesson for the future – also by the fact that criminal law, by its very nature, is unable to deal with the problems of collective guilt or to recognise the suffering of collective victimhood.     

Narratives on World War II in Poland

Memories of the World War II in life stories about its events and experiences are part of every Polish family history. The analysis of life stories of wartime helps to discover the meaning of past events and experiences to individuals, as well as to whole social strata. The role of family affiliations in Polish narratives about the war is briefly discussed, and chosen details of historical background are characterized in one case, using the biographical narrative interview. Two sisters, when telling their own biographies, present the history of their family. Their reasons for concentrating on the collective aspect of their war experiences are explained, and significant social and historical processes exemplified in their story are described. The sisters' family history represents a part of tradition that has been forgotten in postwar Polish society. Pauperization of the landowner stratum prevented the process of transmitting this part of cultural heritage to forthcoming generations. Communist authorities welcomed this process of forgetting, whereas the important aspect of Polish cultural and social heritage based on the lifestyle of the countryside estates was emasculated.     

The Language of the UN: Vagueness in Security Council Resolutions Relating to the Second Gulf War

Over the last few years the diplomatic language of UN resolutions has repeatedly been questioned for the excessive presence of vagueness. The use of vague terms could be connected to the genre of diplomatic texts, as resolutions should be applicable to every international contingency and used to mitigate tensions between different legal cultures. However, excessive vagueness could also lead to biased or even strategically-motivated interpretations of resolutions, undermining their legal impact and triggering conflicts instead of diplomatic solutions. This study aims at investigating intentional vagueness in Security Council resolutions, by focussing on the analysis of the resolutions relating to the second Gulf war. Using the qualitative Discourse-Historical approach (Wodak in Rhetorics of racism and antisemitism, Taylor & Francis Ltd., London [2000]) and quantitative analysis tools (Antconc and Sketch Engine), special attention is given to the historical/political consequences of the vagueness and indeterminacy used in that framework and to the study of vague ‘weasel words’ (Mellinkoff in The language of the law, Little, Brown & Company, Boston [1963]), modals, and adjectives contained in the corpus. The hypothesis of intentional vagueness is further reinforced through an analysis of the US legislation related to the outbreak of the war, to reveal how the US has legally interpreted UN legislation and to understand the purposes and consequences of vague language contained in it. The findings indicate that vagueness in resolutions has triggered the Iraqi conflict instead of diplomatic solutions with the overall legislative intent of using intentional vagueness as a political strategy.     

The ‘Impossibility’ of Maritime Neutrality During World War 1

Netherlands International Law Review -     

The other side of the Anne Frank story: The Dutch role in the persecution of the Jews in World War Two

Contrary to their historical reputation there were only a few Dutch citizens who rescued Jews during World War II by providing their homes as a shelter. Of all the West European countries occupied by the Germans, the number of Jewish casualties was the highest in The Netherlands. Before the occupation there were approximately 140,000 Jews living in The Netherlands, and of that total at least 109,000 were murdered. When the war was over many war criminals and Nazi collaborators were punished. This did not include, however, Dutch civil servants and many of the police and train conductors who were all part of the Holocaust machine. They were recruited from the large middle-ground of a population of mainly bystanders. In addition, Dutch criminology has been conspicuously silent about the Holocaust. Therefore, I shall discuss the following issues as contributory to the Holocaust: (1) the denunciation process; (2) the attitude of The Netherlands' bureaucracy in general; and, (3) the negative impact of the actions of officials in positions of the highest power such as the Queen (who fled to Britain), members of the Supreme Court and Chiefs of Police.     

The history of forensic medicine in Hungary

The XIIIth Congress of the International Academy of Forensic and Social Medicine will be held in September 1985, in Budapest, and for this occasion we gave a brief account of the past and present state of forensic medicine in Hungary.     

The Commission on the Responsibility of the Authors of the War and on Enforcement of Penalties and its Contribution to International Criminal Justice After World War II

This article traces the evolution of discussions within the Commission on the Responsibility of the Authors of the War and on Enforcement of Penalties and the United Nations War Crimes Commission regarding the establishment of an international criminal court. The Commission on the Responsibility of the Authors of the War and on Enforcement of Penalties was the first war crimes commission that seriously debated establishing an international criminal court for the prosecution of war criminals. Following the discussions held in the UNWCC, the International Military Tribunal in Nuremberg was created. All three institutions played a major part in the development of international criminal law.