不同死后时间组织中吗啡免疫组化检测的初步研究

目的检测不同死后时间组织中的吗啡。方法应用抗吗啡兔血和SABC法对不同死后时间大鼠肝肾组织中吗啡进行了检测。结果初步发现死后27h仍可在肝肾组织普通石蜡切片上检出阳性染色。结论免疫组化技术可以用以检测死后组织中的毒物。     

不同死后时间组织中吗啡免疫组化检测的初步研究

目的 检测不同死后时间组织中的吗啡。方法 应用抗吗啡兔血和ＳＡＢＣ法对不同死后时间大鼠肝肾组织中吗啡进行了检测。结果 初步发现死后２７ｈ仍可在肝肾细胞普通石蜡切片上检出阳性染色。结论 免疫组化技术可以检测死后组织中的毒物。     

根据蝇类蛆长度变化系数θ推断尸体死亡时间

目的通过动物实验,探寻嗜尸蝇类蛆的生长长度与尸体死亡时间之间的关系,从而为判断尸体死亡时间提供参考依据。方法将实验用犬击毙后观察尸蝇来到犬尸上的时间、产卵时间、卵期、蛆增长到规定长度所需时间。结果证实蛆虫长度与尸体死亡时间存在正相关性,并进而推算出相关系数θ,从而推导出了一个通过蛆虫长度计算尸体死亡时间的公式。结论通过实际案例的验证,通过该公式可以计算出尸体的大致死亡时间。     

Occurrence of myocarditis in sudden death in children.

This study suggests that the prevalence of "silent" myocarditis may be higher in the pediatric population than is generally suspected and may contribute to a significant number of sudden and unexpected deaths in children, particularly those older than one year of age. The incidence of histologic myocarditis in children dying a violent death is similar to that reported as an incidental finding in adults.     

根据肝脏死后改变推断死亡时间的研究进展

机体死后会发生一系列的改变,是腐败的过程,也是器官组织细胞自溶的过程。在司法实践中,通过研究肝脏死后改变,可以更准确的推断死亡时间,为案件的侦破提供线索。本文综述了近几十年来,利用肝脏死后变化推断死亡时间的研究进展。     

Pathologico-anatomic findings in sudden, unexpected death in children and adults with influenza A infection

Thé following observations resulted from studies on forensic autopsy cases: In 76% of the adults and 55% of the infants the cases of unexpected sudden death without morphologically verifiable causes of death showed virologic evidence of recent influenza-A (H3N2)-infection. The pathologic findings corresponded with the findings in lethal infections with influenza-A viruses. Investigation of cases of sudden and unexpected death should always include virologic serum tests. The demonstration of IgM antibodies against influenza-A virus confirms that there was a recent infection. Death from influenza-A infections occurs also in the interepidemic periods.     

Serum C-reactive protein levels in postmortem blood--an analysis with special reference to the cause of death and survival time

We have investigated postmortem serum CRP levels in 408 forensic autopsy cases consisting of 216 acute and 192 non-acute death cases having postmortem interval of less than 48 h. CRP ranged from 0.03 to 66.13 mg/dl with the median of 0.28 mg/dl. In 362 traumatic death, survival time and the presence of severe infection were the major factors contributing to CRP elevation, while postmortem interval, age, gender, hepatic injury and liver cirrhosis was not. In almost all the immediate deaths (15/16) CRP remained at a low level (<0.5 mg/dl). Acute deaths and subacute deaths within 6 h showed lower CRP levels compared to longer survivors, consistent with the clinical and experimental studies. As for natural diseases, the CRP level reflected the pathological findings. The results suggest a possibility of CRP as a forensic diagnostic marker.     

Compound muscle action potential analysis in different death models: significance for the estimation of early postmortem interval

In this experimental study, we investigated the varieties of excitability of gastrocnemius muscle via sciatic nerve as per different death models (asphyxia, abundant-bleeding and gradual-bleeding) on rats and the significance for the estimation of postmortem interval was evaluated. For this purpose, the rats were applied different stimulus intensities (5, 20, 40 mA) with 0.1 ms duration, before, during and every 5 min after death, using rectangular impulses, and the mean amplitude, onset latency and area values for each compound muscle action potential (CMAP) were elicited. It was detected that amplitude and area increased and onset latencies prolonged in the first postmortem 15 min. From the 15 min, CMAP area and amplitude showed an ever-increasing decrease and the prolongation of onset latencies became apparent. The decrease rate of area and amplitude was found to be statistically significantly different in asphyxia and abundant-bleeding models compared with in gradual-bleeding model, at 30 min measurements. However, there was not any significant difference in onset latency increase rates of three groups. Separately, any significant correlation between the agony and excitability periods among the groups could not be detected. The fact that the increase rate of onset latency did not show a significant difference as per death models indicated that onset latency ratios would be more appropriate criteria in determination of postmortem interval, regardless the reason of death.     

家兔死后眼玻璃体液消光度与死亡时间关系研究

目的 为寻找一种精确推定死亡时间的方法。方法 应用 754分光光度计,在 420nm波长下,检测了 48只家兔死后不同时间的眼玻璃体液消光度,探讨其与死亡时间的关系。结果 眼玻璃体液消光度（ X）与死亡时间（ Y）呈线性正相关（ r=0.983 27, P< 0.05）,在死后 0～ 72小时,眼玻璃体液消光度与死亡时间存在回归关系（ F=116.54, P< 0.05）,直线回归方程为： Y = 453.30X＋ 0.75 [Y为死亡时间 (PMI),单位为小时; X为眼玻璃体液消光度 ]。结论眼玻璃体液消光度的测定可作为推断死后 72小时内死亡时间的参考指标之一。     

Estimation of postmortem interval. Temperature-correction of extracellular abdominal impedance during the first 21 days of death

Extracellular impedance of the intact abdomen of rats increased from 79.0+/-7.4Omega, 1h postmortem (i.e. 0.04 day), to 130.5+/-14.4Omega at postmortem interval (PMI)=1 day. Impedance then decreased with time, reaching 64.2+/-9.9Omega at PMI=21 days. The time taken for mean abdominal impedance to decrease below the value at PMI=0.04 day averaged 16 days. It is therefore impossible, using extracellular abdominal impedance alone, to distinguish (in terms of interpolating PMI) between numerically equal impedances on the rising and falling phases of curves depicting impedance as a function of PMI.Correction of measured impedances to their theoretically-predicted values at an arbitrarily chosen temperature of 40 degrees C appreciably diminished the magnitude of the increase in impedance following death. Thus, temperature-corrected abdominal impedance increased from 56.2+/-4.8Omega at PMI=0.04 day to 59.5+/-6.2Omega at PMI=1 day. Impedance then decreased, reaching 29.2+/-4.1Omega at PMI=21 days. The time taken for mean, temperature-corrected abdominal impedance to decrease below the value at PMI=0.04 day averaged 3 days (as opposed to 16 days (see above) in the absence of temperature-correction).These findings are believed to improve the usefulness of extracellular abdominal impedance as a potential tool for estimation of postmortem interval.     

Free amino acid concentrations in vitreous humor and cerebrospinal fluid in relation to the cause of death and postmortem interval

We studied free amino acids in vitreous humor and cerebrospinal fluid from 58 cadavers in the course of routine medicolegal autopsies in the city of Granada. The main objective was to establish whether free amino acids contents in these fluids were related with the cause of death, postmortem interval, and severity of the classic signs of asphyxia. The amino acids (aspartic acid, glutamic acid, serine, glutamine, glycine/threonine/histidine, citruline, arginine, alanine, taurine, GABA, tirosine, valine, methionine, isoleucine, phenylalanine/tryptophan, leucine, and lysine) were quantified by high performance liquid chromatography. There were no statistically significant differences in amino acids concentrations in vitreous humor when the different causes of death were considered. Our results did not show any statistically significant relationship when asphyxial score was plotted against the vitreous content of each amino acid. A statistically significant increase with postmortem interval was observed in vitreous taurine (r = 0.3191, p = 0.01461), glutamate (r = 0.4323, p = 0.0007) and particularly in aspartate (r = 0.4508, p = 0.0003).     

Analysis of gastrocnemius compound muscle action potential in rat after death: significance for the estimation of early postmortem interval

The estimation of postmortem interval is of great importance in forensic medicine. Changes in the properties of excitable tissue provide another possible means by which the time of death can be estimated. This paper reports the monitorization of the compound action potentials recorded from gastrocnemius muscle by means of sciatic nerve stimulation in rats before and after death. The sciatic nerve was stimulated using rectangular impulses of 0.1ms duration and intensities ranged between 1 and 100mA while the rat was alive. Subsequently, the rat was killed by cervical dislocation. The similar measurement procedure was performed at the moment of death and every 5min after sequentially. There was a progressive decline in amplitude values that began 10min after death. The decrease in the amplitude of the compound muscle action potentials (CMAP) was most prominent especially when elicited with lower stimulus intensities. The mean area of the CMAPs also began to decrease beginning from 15min after death. Fifteen minutes after death, the motor latencies began to prolong. Thirty-five minutes after death, the decline in amplitude and area of mean CMAP was most prominent as the mean motor latency. At the 40th minute, most of the CMAPs were unelicitable. During the early postmortem interval, these amplitude, area and motor latency alterations decrease in the amplitude and area, prolongation of motor latency seems to be well correlated with each other and this was statistically significant. These findings are discussed as possible basis of a forensic method for postmortem interval estimation.     

儿童及青少年组非意外性暴力死特点分析

对１０３例儿童及青少年非意外性暴力死进行特点分析。儿童组中学龄前儿童（２～６岁）１２例（均为女性），学龄儿童（７～１２岁）２２例，青少年组（１３～１９岁）６９例。儿童组和１６～１９岁青少年为两个年龄高峰。死亡性质：凶杀占４７．６％（儿童和青少年女性占８９．８％），伤害占２９．１％（男性占９６．７％），余为自杀和不明。死亡原因以机械性窒息（３６．９％）和锐器伤（３４．０％）为多。并就伤害案件的意义及尸检问题进行了讨论。     

Sudden death in children due to mesenteric defect and mesenteric cyst.

Two cases of sudden death in young female children due to small intestinal volvulus are reported, which involved a mesenteric defect and a mesenteric cyst. Death due to small intestinal volvulus is uncommon, especially when complicated by either a mesenteric defect or cyst.     

The autodegradation of deoxyribonucleic acid (DNA) in human rib bone and its relationship to the time interval since death

This research explored the feasibility of using the degradation rate of deoxyribonucleic acid (DNA) in human rib bone to determine the time interval since death. Postmortem human rib samples were surface sterilized and incubated under sterile conditions in either high or low humidity conditions at room temperature for a period of weeks. At selected times, portions of the bone were cut away, and the DNA from these samples was extracted and subjected to strand separating gel electrophoresis. The DNAs in the gels were transferred to a nylon membrane, preserving their relative positions as in the gel, and probed with radioactive total genomic human DNA. Autoradiograms produced were scanned and digitized. When the samples were incubated under identical conditions, the degradation rate of DNA in samples from different individuals appeared very similar. The DNA degradation rate may vary with temperature and humidity more than it varies between individuals.     

Determination of cause and mode of death before and after medicolegal autopsy: a comparative study

Two hundred and twenty-eight consecutive medicolegal autopsies were studied as to the cause of death established by a physician, the mode of death as suggested in the police report, and findings of medical interest often discovered following autopsy. Corrections had to be made even in some cases which seemed to be obvious. With a decrease of the number of autopsies, the records of various registration bureaus would be affected. Autopsies are still necessary for control and correction of clinical causes of death.     

Reports of our death are greatly exaggerated: A study of criminal justice academic program survival in Alabama

It has been suggested that the reduction of and eventual termination of LEEP funds for the education of inservice justice system employees would lead to a reduction in the size of criminal justice academic programs with attendant termination of many programs. However, academic programs are rarely terminated and tend to develop their own constituencies. This study examined the survivability of criminal justice academic programs in Alabama and found that while there may have been a reduction in size at some point, the criminal justice programs continue to survive, are experiencing growth in student population, and have longterm plans for program development.     

Parole survival and legislated change: A before/after study of parole revocation decision making

Past studies of juveniles' attitudes toward the police suggest a single-cause model that implicates personal interactions with the police. We propose that attitudes toward authority and agents of social control develop in a larger, sociocultural context. Specifically we hypothesize that juveniles' attitudes develop as a function of socialization in their communities' social environment, of their deviant subcultural “preferences,” and of the prior effect of these sociocultural factors on juveniles' contacts with the police. We conducted analyses addressing these hypotheses with a population of males sampled within stratified populations of known delinquents. We found that social background variables, particularly minority status, and subcultural preferences, particularly commitment to delinquent norms, affected juveniles' attitudes toward the police both directly and indirectly (through police-juvenile interactions). We consider directions for improving police relations with juveniles in the context of apparent sociocultural and experiential contingencies to attitude development.