DNA identification of skeletal remains by investigator’s intuition

In June 2006 a decapitated woman was found in a parking area of the motorway in the area of Prato (Florence). Since the body was beheaded and no victim’s documents or objects were present at the crime scene, identification at that time was impossible. However, DNA profile from woman’s bones were collected. In the same year (2003), a mother had reported her daughter's disappearance but the two events were not related at that time. About ten years later the mother’s DNA profile was finally acquired for a genetic identification of another girl’s body found in the Ferrara area. These genetic profiles were completely discordant. All these genetic comparisons were carried out on behalf of the prosecutors of the cities involved in the findings of the bodies and in the disappearance complaints, but due to the lack of a database the events remained disconnected. In January 2017, the head of the scientific police of Prato who had followed the investigation and questioned the mother of the missing girl found about ten years later, suggested to the magistrate to order the comparison of the mother's DNA with the genetic profile of the bones found in 2006. This comparison finally allowed the identification of the missing daughter.This story highlights the importance of having forensic DNA database to search for missing persons and how the investigator's intuition can play a key role in resolving criminal cases. In fact, databases of unknown bodies and relatives of missing persons were created in Italy as a part of national DNA database just at the beginning of 2018.     

Does methodology matter in eyewitness identification research? The effect of live versus video exposure on eyewitness identification accuracy

The present study examined the effect of mode of target exposure (live versus video) on eyewitness identification accuracy. Adult participants (N=104) were exposed to a staged crime that they witnessed either live or on videotape. Participants were then asked to rate their stress and arousal levels prior to being presented with either a target-present or -absent simultaneous lineup. Across target-present and -absent lineups, mode of target exposure did not have a significant effect on identification accuracy. However, mode of target exposure was found to have a significant effect on stress and arousal levels. Participants who witnessed the crime live had higher levels of stress and arousal than those who were exposed to the videotaped crime. A higher level of arousal was significantly related to poorer identification accuracy for those in the video condition. For participants in the live condition however, stress and arousal had no effect on eyewitness identification accuracy. Implications of these findings in regards to the generalizability of laboratory-based research on eyewitness testimony to real-life crime are discussed.     

Individual identification of cats and dogs using mitochondrial DNA tandem repeats?

Cats and dogs are very common animals in the human environment. In Switzerland, one in five households owns a cat or a dog. Their hairs are very easily transferred and could be used as a frequent trace evidence. Using DNA analysis, identification of these animals should be possible as it is in human identification. However, most of the time, no nuclear DNA can be recovered from the hair. It is therefore necessary to rely on mtDNA. Cats and dogs have tandemly repeated sequences in their mtDNA control region. In this study, the authors show that these tandem repeats are very polymorphic but are also the source of a very high level of heteroplasmy. The authors, therefore, examined if this might prevent their use in forensic identification.     

Developmental validation of the SPERM HY-LITER™ kit for the identification of human spermatozoa in forensic samples

Abstract: With sexual assault evidence, the visualization of spermatozoa confirms that ejaculation has occurred. However, microscopic examination of spermatozoa is a laborious process and can sometimes result in sperm cells being overlooked. Here, we present the developmental validation of the SPERM HY‐LITER? kit, which contains a human sperm–specific mouse monoclonal antibody coupled to a fluorescent Alexa 488 dye. The kit was tested using samples of human semen, saliva, blood, and urine, various animal semen extracts, sexual lubricants, and a commercially available spermicidal film. Postcoital vaginal swabs, degraded semen samples, and samples prepared with sample fixation techniques that deviated from the kit‐provided protocol were also tested. In each case, the SPERM HY‐LITER? kit was demonstrated to bind only to human sperm cell heads. Limitations to this fluorescent staining procedure include nonspecific staining and increased background fluorescence with extreme heat fixation in some samples.     

Validation study of KPICS SpermFinder™ by NicheVision Forensics, LLC for the identification of human spermatozoa

Abstract: Microscopic analysis for the identification of spermatozoa is commonly performed during the forensic examination of sexual assault evidence. Two widely utilized methods for the confirmation of the presence of spermatozoa are visualization of the cells via phase‐contrast microscopy with wet mounted samples and bright field microscopy with histologically stained samples. The KPICS SpermFinder? by NicheVision Forensics, LLC accelerates this time‐consuming process via an automated microscope with an algorithm designed to locate spermatozoa on a Christmas tree histologically stained microscope slide. Upon a qualified scientist’s review of the generated data, the KPICS SpermFinder? was able to locate spermatozoa, typically finding on average 106.28% ± 115.37% more spermatozoa than with manual examinations. The KPICS SpermFinder? provided the location of identified cells with reproducible results.     

Analysis of some commonly found inhalants using gas chromatography-mass spectrometry (GC MS) – Effect of substrates on the identification of inhalants

Inhalant abuse is a serious and ever-evolving problem for our society. Inhalants are abused more commonly by teenagers and adolescents. Inhalants such as glue, paint thinners, correction fluid, and nail paint remover are easily available in the market which makes them readily abused. Out of various methods of abuse, huffing (placing a piece of cloth soaked with inhalant is placed in the mouth), bagging (placing head in a polythene bag containing inhalant), and ballooning (balloons filled with inhalant are used for inhaling vapors through the mouth) are most common. Inhalant abuse results in multiple target organ dysfunction with neuritis and brain damage due to the dissolution of the myelin sheath. In acute poisoning cases, it might cause death due to asphyxia and sudden sniffing syndrome. In such cases, various articles containing traces of inhalants could be retrieved from the scene of incidence and to detect these traces become an important facet of the investigation. However, it might be difficult due to the rapid evaporation of inhalants and the formation of reaction product species. In the present study, an attempt has been made to study the effect of three substrates (balloon, cloth, polythene) on component profiles of four paint thinners and four nail paint removers. The study suggests that although some components are retained, there is the formation of a large number of reaction product species and these must be taken into consideration before furnishing the opinion. These reaction product species might also be inhalant specific which must be further explored.     

What shoe was that? The use of computerised image database to assist in identification

The use of a computerised (digital image-based) reference system for the capture, storage and retrieval of shoe soles and uppers has the ability to give quick and reliable information to the investigator in relation to the brand and model of shoe responsible for latent impressions located at crime scenes. The success of the system described is due to the diversity and simplicity of the classification codes, coupled with the ability to search part or all of a shoe sole area. This gives the user greater discriminating power and, with the use of icons and ‘click on’ features, makes the system user friendly when classifying and searching for shoes, thus reducing the possibility of interpretation error.     

Can training improve eyewitness identification? The effect of internal feature focus on memory for faces

Evidence suggests that individuals rely on external features such as hair and face shape when processing and recognising unfamiliar faces. This is problematic in a forensic setting because hairstyle can be changed easily. In two studies we examined whether training individuals to focus more on the stable, internal features of the face (e.g. eyes, nose and mouth) and hence less on the changeable external features would improve identification accuracy. In Study 1, participants were instructed to focus on: (1) internal features, (2) external features, or (3) both internal and external features. Participants’ eye movements were monitored while they studied target faces, which they later tried to identify from an array that included the target with a changed appearance. In Study 2, participants were instructed to focus on: (1) internal features, (2) external features, or (3) given no instructions. Participants were required to identify target faces from line-ups, where they were presented either unchanged or with an altered hairstyle. Results suggested that training individuals to attend to internal features of unfamiliar faces may improve identification accuracy in the common situation where external features have changed between presentation and identification. However, internal feature focus may decrease identification accuracy when external features are unchanged.     

How leadership enhances employees’ knowledge sharing: the intervening roles of relational and organizational identification

Knowledge exchange among employees is crucial to organizational effectiveness. Leadership can enhance or detract from employees’ willingness to share knowledge. This study examines how leadership affects knowledge sharing in a knowledge-intensive work setting. It proposes and tests a model which posits that (1) transformational leadership affects the extent to which employees identify with their manager; (2) this relational identification, mediated by the quality of LMX (Leader-Member Exchange), leads to greater identification with the organization and its goals, which in turn results in greater knowledge sharing. The sample consisted of two hundred and three R&D employees engaged in advanced technological projects. Path analysis results indicated that there are both direct and indirect (through LMX) relationships between transformational leadership and relational identification: relational identification promotes organizational identification which, in turn, is positively related to knowledge sharing. These results highlight the importance of transformational leadership and LMX for promoting relational and organizational identification, thereby facilitating employee knowledge sharing.     

Rapid and non-destructive identification of claws using ATR-FTIR spectroscopy–A novel approach in wildlife forensics

Differentiation and identification of Royal Bengal Tiger (Panthera tigris tigris) and Indian Leopard (Panthera pardus fusca) claws is a challenging task in wildlife forensics, due to similarity in their morphology, anatomy and chemical compositions as both the species are closely related to each other genetically. ATR-FTIR spectroscopy, which offers a non-destructive and safe alternative technique to other conventional methods, has been employed in the present work to differentiate claws of Royal Bengal Tiger and Indian Leopard. An attempt has been made to differentiate 31 reference claw samples from 16 different Royal Bengal Tigers, 15 different Indian Leopards, and 10 fake claws using ATR-FTIR spectroscopy supplemented with PCA, PLS-DA, and LDA. PCA could not distinguish the samples of two closely related species among themselves as well as from the fake claws. On the other hand, PLS-DA and LDA models both yielded highly significant classification rate for differentiation among the samples of Royal Bengal Tiger, Indian Leopard, and their fake counterparts. Further, seven blind claw samples that were pretended to be unknown to the analyst of both the species are also examined and identified correctly to their respective groups. The R-Square value obtained for PLS-DA model to differentiate Royal Bengal Tiger, Indian Leopard, and fake claws is 0.99, which is highly significant for predictive accuracy. This study shows that ATR-FTIR spectroscopy with PLS-DA/LDA has a potential to present a rapid, non-destructive, reliable, and eco-friendly approach for the accurate identification and differentiation of Royal Bengal Tiger and Indian Leopard claws.     

Is re-creation of human identity possible using tooth prints? An experimental study to aid in identification

Enamel in teeth, as documented is the hardest substance in the entire human body. Tooth prints are the enamel rod end patterns on the tooth surface. Could this fact be a boon in utilizing it for an individual's identification in the hands of forensic experts? A study has been attempted towards the same. These tooth prints were recorded for 60 extracted tooth specimens after acid etching using a cellophane tape. Subsequently a digital image of the print was obtained which was subjected to biometric conversion using Verifinger standard SDK version 6.0 software followed by the use of Automated Fingerprint Identification System (AFIS) software for comparison of the tooth prints. In results we observed that tooth prints were composed of varied patterns and sub-patterns. A comparison was made between the tooth prints of different and the same individual and also between different classes of teeth (incisors, canines, premolars and molars). None of the patterns exhibited intra- and inter-individual similarity. Nor did any particular class of the tooth could be preferentially used over the other for an individual identification. This field demands a need for further exploration towards the use of tooth prints for establishing an individual's identity.     

A “concrete” identification in a dismemberment case

Homicide perpetrators can use concrete as a means to conceal their victims. When concrete encasement is encountered in the forensic field, albeit rarely, it is often coupled with postmortem dismemberment. This method of obscuring the evidence presents unique investigative obstacles, specifically related to identification. Various approaches to obtaining fingerprints from decedents encased in concrete have been suggested and implemented over the years. The presented case is that of an initially unidentified 44-year-old male, who was subject to postmortem dismemberment and concrete encasement. Meticulous excavation techniques facilitated preservation of evidence and an anatomical reconstruction of the body. These techniques enabled inspection of the incision sites of the dismembered remains during the postmortem examination. Identifiable jewelry and tattoos were noted at autopsy. Further, the resulting concrete molds could be utilized to obtain fingerprints. These prints were used to ultimately identify the decedent.     

The GDPR: A game changer for electronic identification schemes? The case study of Gov.UK Verify

This article offers an interdisciplinary analysis of the General Data Protection Regulation (GDPR) in the context of electronic identification schemes. Gov.UK Verify, the UK Government's electronic identification scheme, and its compatibility with some important aspects of EU data protection law are reviewed. An in-depth examination of Gov.UK Verify's architecture and the most significant constituent elements of both the Data Protection Directive and the imminent GDPR – notably the legitimising grounds for the processing of personal data and the doctrine of joint controllership – highlight several flaws inherent in the Gov.UK Verify's development and mode of operation. This article advances the argument that Gov.UK Verify is incompatible with some major substantive provisions of the EU Data Protection Framework. It also provides some general insight as to how to interpret the requirement of a legitimate legal basis and the doctrine of joint controllership. It ultimately suggests that the choice of the appropriate legal basis should depend upon a holistic approach to the relationship between the actors involved in the processing activities.     

Simultaneous identification and δ¹³C classification of drugs using GC with concurrent single quadrupole and isotope ratio mass spectrometers

In this study, δ13C values of six cocaine samples were identified and classified using a single quadrupole mass spectrometer and an isotope ratio mass spectrometry (IRMS) as simultaneous gas chromatography detectors. Our instrument modification is simple to use and is useful (i) when the sample is of limited size or can only be injected once, (ii) to help identify peaks in a complicated IRMS chromatogram, and (iii) to help differentiate very simple systems when impurity profiling is not possible. The EI-MS confirmed the identity of cocaine in each sample. The IRMS data distinguished 12 of the 15 possible pair-wise comparisons at the 95% CL. Three samples could not be differentiated by their δ13C ratios for cocaine. ANOVA demonstrated that the measurement variance was consistently larger than the sample variance. As the δ13C values clearly show, this technique enables the exclusion of a potential common source even when two samples have otherwise identical chemical and physical properties.     

“Identified”, “probable”, “possible” or “exclude”: The influence of task-irrelevant information on forensic odontology identification opinion

In a mass disaster situation, identification of the deceased utilising comparison of dental features is frequently heavily relied upon to facilitate rapid and accurate outcomes. The method consists of the comparison of clinical and radiographic records depicting oral structures and dentition to allow an opinion to be produced on a presumed identity. Current forensic odontology identification opinions are expressed as categories of levels of identification. Categories such as “Identified”, “Probable”, “Possible” and “Exclude” are used in various forensic odontology identification scales. The boundaries between the levels of the scales are not fixed; hence, category selection is highly subjective. It is uncertain how extrinsic factors such as exposure to contextual task-irrelevant information or operator experience influence category selection. In this study, forensic odontologist and dentist participants read task-irrelevant context case information containing either strong or weak identification or non-identification suggestions before evaluating and comparing pairs of true matching and non-matching dental radiographs. They were then asked to form an opinion regarding identification using one of four categories from the INTERPOL scale. Context information was found to influence categorical decisions. The magnitude and direction of influence depended on the type of participant, the true match status of the radiographs, and the strength and direction of bias of the context. The results of this study demonstrate the contextual effect and fluidity of the boundaries between the categories on the identification scale and highlight the need for stringent protocols to be developed regarding the use of these categorical scales to enable decision making to be more objective.