不同温度下大鼠脑组织RNA降解与早期PMI的相关性

目的探讨大鼠脑组织8种RNA指标,在不同温度下的表达水平与早期死亡时间(PMI)的相关性。方法将222只SD大鼠随机分为对照组(死后0 h)和4个实验组,实验组断颈处死后分别置于5℃、15℃、25℃和35℃的环境中,于死后1~24 h内9个时间点提取脑组织RNA,利用实时荧光定量PCR技术检测8种RNA指标(β-actin、GAPDH、RPS29、18S rRNA、5S rRNA、U6 snRNA、miRNA-9及mi RNA-125b)的表达水平,ge Norm软件选取合适内参,SPSS软件对内参标准化RNA指标进行回归分析,R软件构建推断PMI的数学模型,另选6只已知PMI的SD大鼠予以验证。结果 5S rRNA、miR-9和mi R-125b表达稳定,可作为内参指标。β-actin和GAPDH具有良好的时序性降解规律,在24 h内随PMI延长不断降解。R软件拟合得ΔCt值随PMI和温度变化的数学模型可用以推断PMI。运用β-actin和GAPDH验证模型的平均误差率分别为14.1%和22.2%。结论β-actin和GAPDH表达水平与PMI和环境温度相关性良好。本研究建立的数学模型可为温度变化条件下的早期PMI推断提供参考。     

实验性脑挫伤后神经元和星形胶质细胞反应的时间性变化

运用免疫组化技术，以神经元特异性烯酸酶（NSE）和胶质纤维酸性蛋白（GFAP）作为标记物，观察大鼠实验性脑挫伤后不同存活时间中神经元和星形胶质细胞的数量变化。结果发现，伤后3h可见NSE和GFAP染色变化，且随着存活时间延长，脑皮质内挫伤处周围神经元逐步减少，而星形胶质细胞则逐渐增加，具有一定的时间规律性。为脑挫伤后早期存活时间的推断提供了新的方法。     

大鼠脑、骨髓细胞核DNA降解推断死后间隔时间的研究

目的检测大鼠死后不同温度下脑、骨髓细胞核DNA降解规律，寻找推断早期死亡间隔时间（PMI）的新参数。方法10℃和20℃下，大鼠死后0～40h内，每隔4h取材脑组织和骨髓，单细胞凝胶电泳（SCGE）检测DNA降解程度，线性回归分析比较彗星参数HeadDNA％、尾长（TL）、Olive尾矩（TM）与PMI的关系。结果大鼠死后早期脑细胞、骨髓细胞核Head DNA％随着PMI逐渐下降的程度不同，20℃脑细胞核Head DNA％降解速率较快。与骨髓细胞相比，脑细胞核Head DNA％与PMI线性关系较好。与TL、TM相比，Head DNA％与PMI的线性关系较好。结论脑组织是利用SCGE检测DNA降解推断PMI的合适检材。Head DNA％较TL、TM推断PMI的价值更高。     

人体脑组织RNA表达水平与早期PMI的相关性

目的探讨人体脑组织多种RNA指标的表达水平与早期死亡时间(PMI)的相关性。方法选取12例已知PMI(4.3~22.5 h)的个体,提取脑组织总RNA。选取8种常用RNA指标(β-actin、GAPDH、RPS29、18S rRNA、5S rRNA、U6 snRNA、miRNA-9、miRNA-125b),通过实时荧光定量PCR技术检测其在脑组织中的表达水平。运用geNorm软件筛选早期PMI表达稳定的内参指标,并分析其表达水平与相关因素(年龄、性别、死亡原因)的关系。运用R软件将内参标准化的RNA指标代入前期研究建立的数学模型推断PMI,计算推断PMI的误差率以验证模型精确性。结果 5S rRNA、miRNA-9和miRNA-125b表达稳定,其表达水平与年龄、性别、死亡原因无关。利用β-actin推断PMI的误差率为24.6%,GAPDH为41.0%。结论 5S rRNA、miRNA-9和miRNA-125b在死亡早期表达稳定,适合作为人体脑组织的内参指标。β-actin表达水平与PMI相关性良好,有望成为推测早期PMI的辅助指标。     

迟发性外伤性脑内血肿脑组织GFAP、S-100和NSE的免疫组织化学研究

应用免疫组织化学ABC法和S－P法，对6例伤后1．5天至2年发生的迟发性外伤性脑内血肿（DTICH）脑组织标本进行神经胶质细胞内胶质纤维酸性蛋白（GFAP）、S－100蛋白（S－100）和神经元细胞内特异性烯醇化酶（NSE）变化和应用价值的研究。尸检和组织学检查发现有脑挫伤等病变。6例DTICH脑组织挫伤及周围区均有明显的GFAP、S－100阳性的细胞变化，细胞数目增多，胞体肥大、增生，免疫组化反应产物增多、深染；神经元细胞NSE免疫组化染色均呈阴性改变。结果表明：6例DTICH脑组织中GFAP、S－100的NSE的免疫组织化学变化是有一定时间间隔的外伤性陈旧性改变，在DTICH的病理诊断上具有重要价值，可作为DTICH法医学鉴定的一个辅助手段。     

大鼠死后管家基因mRNA稳定性及其时序性降解与PMI相关性研究

目的研究脑组织及骨骼肌内β-actin、GAPDH、RPL32、PKG1、SDHA、RPL13、HPRT、TBP、YWHAZ死后稳定性及其相对表达量与死亡时间(PMI)的相关性。方法取健康成年SD大鼠33只,依据据死亡时间不同随机分为11组(0h、1h、3h、6h、12h、24h、2d、4d、8d、16d、24d),分别在各时间点取大鼠胫骨前肌及脑组织约50mg,提取各组织总RNA,利用RT-q PCR技术检测9种管家基因的m RNA相对表达量。运用ge Norm、Norm Finder和Best Keeper软件对基因稳定性进行评价,利用spss软件对内参标准化的m RNA指标进行回归分析。结果脑组织及骨骼肌中均为HPRT稳定性最高,适合用于本研究的内参基因。脑组织中SDHA、RPL32及TBP 3种指标的相对表达量变化与PMI有一定的线性关系。骨骼肌中m RNA指标相对表达量与PMI之间没有显著的相关性。结论脑组织及骨骼肌是较适宜推断晚期死亡时间的检材。脑组织中SDHA、RPL32及TBP 3种m RNA的相对表达量变化与PMI有一定的线性关系,有望成为推断PMI的辅助指标。     

脑损伤时间推断研究新进展

脑损伤后经过时间的推断,是多年来国内外法医学界积极研究的课题。本文拟从免疫组化及分子生物学的角度,将下列几个指标的新进展与脑损伤时间推断的关系进行综述,以期为法医病理学推断脑损伤时间提供一些可行性指标。1神经元特异性馈醇酶（neuronspecificenolase,NSE）NSE是神经元的特异性标志酶,用NSE作标记物可以从数量上和形态上研究脑损伤中神经元的变化。存活的神经元NSE染色呈阳性,变性坏死的神经元NSE呈阴性或弱阳性,说明神经元内NSE染色的变化与脑损伤有明显的相关性。周高举等[1]将NSE应用于脑挫伤后的时间推断,发…     

大鼠骨骼肌新鲜度指标与早期死亡时间关系

目的 计算不同时间大鼠骨骼肌新鲜度指标(K值、K1值、K0值、H值、P值、G值和IMP比例)变化规律,研究其与早期PMI的关系,筛选出最优评价指标,用于早期PMI推断.方法 SD大鼠处死后,保存在20℃恒温条件下,于死后即刻(0h)、4h、8h、16h、24h、32h和48h提取大鼠双后腿骨骼肌,利用高效液相色谱仪测定...     

混合效应模型在玻璃体液推断死亡时间中的应用

目的通过检测不同温度下家兔尸体玻璃体液内K~+、Mg~(2+)浓度随PMI的变化规律,探索应用混合效应模型推断死亡时间(postmortem interval,PMI)的可行性。方法家兔处死后分别置于5℃、15℃、25℃和35℃温度下保存,在0~120h内每12h双眼交替微量提取玻璃体液80~100μL。应用生化免疫分析仪检测玻璃体液中K~+、Mg~(2+)的浓度。应用混合效应模型进行分析拟合,建立PMI推断方程。使用放置于10℃、20℃、30℃温度下均经过20、40、65h的样本检测数据对PMI推断方程进行验证。结果各温度(y)下家兔玻璃体液中K~+、Mg~(2+)浓度[f(x,y)]随PMI(x)延长均呈上升趋势。在5℃~35℃,PMI及温度拟合的K~+、Mg~(2+)浓度方程分别为:f_(K~+)(x,y)=3.413 0+0.309 2 x+0.337 6 y+0.010 83 xy-0.00247x~2(P0.000 1);f_(Mg~(2+))(x,y)=0.745 6+0.006432 x+0.0338y(P0.000 1)。经验证,PMI为0~40 h时,K~+、Mg~(2+)浓度推断PMI的偏离时间均在10 h以内;PMI为40~65 h时,偏离时间在21h以内。结论在5℃~35℃的环境温度区间内,利用混合效应模型拟合的方法可实现利用温度和玻璃体液物质浓度双参数推断PMI,将为解决玻璃体液化学物质在PMI推断中的实际应用提供新方法。     

大鼠脑挫伤后基质金属蛋白酶3的表达

目的 检测脑挫伤修复过程中基质金属蛋白酶3(matrix metalloproteinase-3,MMP-3)的表达,探讨其表达规律与损伤时间的相关性.方法 将大鼠分成对照组、假手术组和脑挫伤组.应用免疫组织化学技术和蛋白质印迹(Western blot)方法检测脑挫伤后不同时间脑组织中MMP-3的表达变化. 结果免疫组化结果:对照组及假手术组脑组织未见MMP-3阳性染色;伤后6h组脑组织出现MMP-3阳性染色,此后24h内染色逐渐增强,伤后5 d阳性细胞数及染色强度均达高峰,随之下降,伤后14d仍有少量MMP-3表达.Western blot结果:对照组及假手术组脑组织未见MMP-3阳性染色;挫伤后6h组出现MMP-3表达,随后表达量逐渐上升,5d达到高峰,以后逐渐下降,14d仍有表达.结论 大鼠脑挫伤修复过程中MMP-3的表达呈时序性变化规律,可望用于脑挫伤时间的推断.     

流浪乞讨与管制--从贫困救助看中央与地方权限的界定

随着《收容遣送办法》的废止,个人自由权利与社会治安秩序之间的现实冲突比过去更为突显。应转变中央和地方关系的传统思维,在允许地方更多自主权和选择权的同时,更充分地保障公民个人的宪法基本权利。无论是中央和地方关系的法律界定,还是公民权利的宪法保障,都要求建立独立的司法机构以审查地方立法的有效性。     

当前广东拐卖妇女儿童犯罪的特点、原因及对策

近年来,广东一些地区拐卖妇女儿童犯罪屡禁不绝,愈演愈烈.拐卖妇女儿童犯罪是一种十分野蛮的犯罪活动,它严重侵害了妇女儿童最基本的人身权利,极大地危害了社会秩序,败坏了社会风气.本文通过分析其犯罪特点及原因,进一步提出相应对策.     

Elderly widows and widowers and their coresidents in late 19th- and early 20th-century England and Wales

This article examines the family and household patterns of widowers and widows in England and Wales between 1891 and 1921 in terms of the proportion of widowers and widows who lived with never- or ever-married children, servants, or inmates, with nonrelatives only, or on their own. The absence of marked change between 1891 and 1921 in the frequency with which elderly widowers and widows lived with a married or never-married child are in line with the Laslett [J. Fam. Hist. 12 (1987) 263.] expectation that family patterns evolve only slowly, certainly much slower than the forces of political and economic change. Where changes can be detected, as in the decline in frequency of coresidence with servants or inmates, these changes were experienced by the younger as well as the older widowed and by married people. Apparently, the residence patterns of the elderly widowed changed due to the introduction of means-tested old-age pensions in 1910. A second investigated is the extent to which the socioeconomic environment influenced the residence patterns of the widowed in a given time period. The effect in general proves to be weak with little difference between one environment and another in the frequency with which widowers coresided with their children and evidence of gender balancing of the household (relatively more widows living with sons and more widowers with daughters) visible only in agricultural environments.     

全球化与法理学的变革和更新

全球化进程深刻地改变着法的存在方式、价值取向和发展方向 ,也必然引起法理学的理论变革与更新。本文以法律本体论、法律价值观、法律人格观、法律发展观、法治观等五个法理学的基本论题为主题 ,分析或展望全球化所可能引起的法理学的理论变革和更新。     

Up and Down,Old and New: Values and Value Systems of MPs and Voters in France

MPs are often criticised as being homogeneous. This is well known in terms of social background or gender, but the criticism also holds for values and norms. MPs are said to share normative agreements on the essential points and demonstrate differences on second-order issues. This criticism is even more widespread regarding the new politics based on the cultural divide, notably vis-à-vis politicians from the extreme right as far as immigration, European integration or globalisation are concerned. In this contribution, these criticisms are addressed by investigating the degree of normative agreements and disagreements of French MPs. Furthermore, the differences both in old and new politics between MPs on the one hand and the electorate as a whole and their supporters on the other hand are evaluated. It is concluded that MPs are definitely not all the same and the degrees of difference among MPs or with the electorate are far from being those expected.     

论抵押与留置担保权的竞合条件及其效力

抵押权与留置权在同一标的物并存的情况下,哪一个应优先受偿的问题,是一个重要然而在法律中尚未明确的问题。本文提出并论证了抵押权与留置权在同一标的上竞合而主体不同的状态下,留置权应优先于抵押权受偿的观点。     

Habermas and Deleuze on Law and Adjudication

This article stages an encounter between Habermas and Deleuze on law, rights, and adjudication. Most of the article is spent developing Habermas’s concept of adjudication as the application of communicatively generated norms. This application, I argue, involves a complex temporality that is at once retrospective and non-creative. Deleuze is used to critique this concept of adjudication in favor of one based on concrete situations and the creation of new problems. In so doing, I will develop Deleuze’s notorious, and notoriously hostile, remarks on human rights and philosophies of communication by relating them to discourse ethics and to the positive conception of law and judgment that can be drawn from his work.

Crime Volume and Law and Order Culture

The literature on public opinion about crime and justice has neglected the exploration of macro‐ or community‐level influences on individual‐level attitudes. A key macrofactor that may be related to individual level attitudes is the volume of violent crime. High crime rates can facilitate the development of a culture of “law and order,” a response that may be a practical or instrumental attempt to control crime. The present paper tests the hypothesis that persons residing in nations marked by a high volume of crime will be more likely to adhere to elements of a law and order culture. It employs data from the International Social Science Program (N = 15,024). Controls are taken from major theoretical perspectives on public opinion about crime as well as demographic factors. The results from a hierarchical linear model support the hypothesis that individuals residing in nations with high crime rates are more likely than others to support law and order ideologies. The findings extend the support for this relationship from research based on the US alone to other industrialized societies.     

International aid,trade and investment and access and allocation

International Environmental Agreements: Politics, Law and Economics - The access to goods and their allocation (who gets what, when, where and how) is one of the analytical problems of the 2009...