A comparison of animal jaws and bite mark patterns

The purpose of this study was to compare the jaw shapes and bite mark patterns of wild and domestic animals to assist investigators in their analysis of animal bite marks. The analyses were made on 12 species in the Order Carnivora housed in the Mammalian Collection at the Field Museum of Natural History in Chicago, Illinois. In addition to metric analysis, one skull from each species was photographed as a representative sample with an ABFO No. 2 scale in place. Bite patterns of the maxillary and mandibular dentition were documented using foamed polystyrene exemplars, which were also photographed. A total of 486 specimens were examined to analyze the jaw and bite mark patterns. A modified technique for measuring intercanine distances was developed to more accurately reflect the characteristics seen in animal bite marks. In it, three separate areas were measured on the canines, rather than just the cusp tip. This was to maximize the amount of information acquired from each skull, specifically to accommodate variances in the depth of bite injuries.     

A technique to capture, analyze, and quantify anterior teeth rotations for application in court cases involving tooth marks

Anterior teeth within the human dentition have a specific numerical rotation value. Bite marks show an array of angled indentations, abrasions, microlacerations, and contusions. These marks generally represent the incisal surfaces of the suspect's dentition reflecting the rotation values of the teeth in the dental arch. This study described a method for capturing and analyzing anterior dental rotations. The rotations of individual anterior teeth within the study population were categorized as common, uncommon, and very uncommon according to Allen's classification. In the absence of a large number of incisal patterns present in a bite mark, a single but heavily weighted tooth rotation could be of equal discriminatory potential to several common rotation values. No prevalence studies quantifying individual tooth rotations are available. The measurement of each individual tooth rotation together with its individual discrimination potential will enhance the evaluation of the concordant features observed in bite marks.     

Effectiveness of comparison overlays generated with DentalPrint software in bite mark analysis

Validation studies of the new DentalPrint software were carried out with experimental bite marks in pigskin. The bite marks were digitally photographed according to the ABFO guidelines for evidence collection. Dental casts used in the experiment were scanned in 3D and 2D, and comparison overlays were generated using DentalPrint and Adobe Photoshop software, respectively. Digitized photographs of the experimental bite marks and the biting edges obtained in the overlays were compared by two different examiners to analyze the impact of training and experience with the two methods. Receiver operating characteristic (ROC) analysis, sensitivity, specificity, and 95% confidence intervals for each cutoff point were calculated. The expert examiner using DentalPrint obtained the best results, with an area under the ROC curve of 0.76 (SE=0.057; CI at 95%=0.652-0.876). Fairly high specificity values were found for DentalPrint, and the best results were obtained for the cutoff value that discriminated between the examiner's response "biter" and the rest of the possible (specificity 97.9%, CI at 95%=93.2%-99.6%). Therefore, the results presented here indicate that DentalPrint is a useful, accurate tool for forensic purposes, although further research on the comparison process is needed to enhance the validity of bite mark analysis.     

Bite Mark Analysis in Foodstuffs and Inanimate Objects and the Underlying Proofs for Validity and Judicial Acceptance

Even though one of the first bite mark cases was Doyle v. State in 1954 (a bitten cheese case), the research has focused on bite marks inflicted in human skin. As published Papers, Case Reports, or Technical Notes can constitute precedents which are relied upon in making the legal arguments and a considerable amount of case law exists in this area, we present a systematic review on bite mark analysis in foodstuffs and inanimate objects and their underlying proofs for validity and judicial acceptance according to Daubert rulings. Results showed that there is vulnerability in these procedures, and it is essential to demand for focus scrutiny on the known error rates when such evidence is presented in trials. These kinds of bite marks are well documented; however, there has been little research in this field knowing that the protocols of analysis and comparison are the responsibility of the forensic odontologists.     

Yet another method for marking incisal edges of teeth for bitemark analysis

Over the years many methods have been proposed and presented for marking or "highlighting" the incisal edges of anterior teeth on a dental stone model -- one of the critical steps in production of transparent overlays for bitemark analysis. A method is presented here that is an extension and refinement of the "paint the edges" method shown at least as early as 1966 by Gustafson. This method uses "invisible ink" and ultraviolet illumination to produce a very high contrast image of the incisal edges of the teeth on the model. The advantages of this new method are that it is nondestructive, it does not leave visible marks on the models, operator subjectivity in selecting the portions of anterior teeth to be highlighted is reduced, and the author submits that this technique lends itself to easy construction of overlays using computer image-processing software such as Adobe Photoshop and ImageJ.     

Experimental Study of Bite Mark Injuries by Digital Analysis

Abstract: This study was based on a morphometric analysis of bite marks starting from the quantitative definition of the anterior teeth by the geometric acquisition of the “injuries,” using bite marks impressed on pig skin and plastic. Each mark was photographed and acquired. A dedicated program automatically supplied the values of the shape factors and the areas of the pattern geometric figures. The values obtained for the homologous samples were compared to the heterologous values. Statistical comparison was made of the sets by linear regression, determining the correlation coefficient and the determination coefficient for each value. Results showed only 4.8% and 2% of overlap between homologous and heterologous values. This study was carried out in attempting to quantitatively define the anterior teeth of the human dentition and the procedure described and the results obtained support the advantage of morphometric studies and computer‐aided programs in this study of bite marks.     

Reconstruction of Deformed Bite Marks Using Affine Transformations

Abstract: Bite marks inflicted on animate and inanimate objects will undergo a certain degree of deformation. This fact remains one of the biggest stumbling blocks when analyzing evidence for court presentation. It has been demonstrated that the reliability of pattern association analysis will not be affected by minimal degrees of warping, shrinkage, and distortion. In this study, affine transformations were applied to bite marks to establish if minimal distortions would affect the mathematically determined relationships of the defined features. In a real case study, it was then tested whether the distorted bite mark matched the dentition of the perpetrator by applying an affine transformation. This was confirmed to be the case. Affine transformations will thus not affect the relationships of the individual features found in bite marks. The numerical calculations validate the reliability of pattern association analysis in the presence of minimal amounts of warping, shrinkage, and distortion.     

法庭科学痕迹检验实验室质量控制的现状及进展

痕迹检验实验室作为法庭科学实验室的重要组成部分,其质量控制活动在遵循普遍原则的基础上,根据自身专业特性,在质量控制方法选择及活动实施上有所侧重.本文在调研国内外关于痕迹检验实验室质量控制研究与实践的基础上,从结果质量控制、人员资格和培训、方法确认、记录、结果报告、出庭等方面对痕迹检验实验室质量控制要求及现状进行综述,旨在探索痕迹检验实验室的质量控制关键技术,尽快建立国内痕迹检验实验室质量控制规范.     

A method for studying knife tool marks on bone

The characteristics of knife tool marks retained on hard tissues can be used to outline the shape and angle of a knife. The purpose of this study was to describe such marks on bone tissues that had been chopped with knives. A chopping stage with a gravity accelerator and a fixed bone platform was designed to reconstruct the chopping action. A digital microscope was also used to measure the knife angle (θ) and retained V-shape tool mark angle (ψ) in a pig skull. The κ value (elasticity coefficient; θ/ψ) was derived and recorded after the knife angle (θ) and the accompanied velocity were compared with the proportional impulsive force of the knife and ψ on the bone. The constant impulsive force revealed a correlation between the V-shape tool mark angle (ψ) and the elasticity coefficient (κ). These results describe the tool marks--crucial in the medicolegal investigation--of a knife on hard tissues.     

A Morphological and Morphometric Study of Bite Marks Caused by Mice (Mus Musculus) on Different Baits for Forensic Purposes

In animal bites, the dental attributes can be fundamental in identifying the marks made by various species on different matrices. Although rodent bite marks have been studied in the context of postmortem interference, little research has used different baits to analyze these marks linking not only specific behavior patterns but also the possibility of structural damage. Twenty mice (Mus musculus) were exposed to different baits to study their bite marks in a controlled model. The known pattern of parallel and multiple grooves has been seen in all baits, but polyvinyl chloride and fiber‐optic cable were significantly different between each other and the other baits. Some baits showed patterns of anchorage of the upper incisors and space between the lower incisors when gnawing. This technical note represents a novel model of analysis where veterinarians and/or dentists may be asked to give an opinion on alleged animal bite marks.     

The experimental and case study of needle marks on the speedometer as the physical evidence for the collision speed analysis

The collision speed is important in accident analysis, and needle marks can be helpful as the physical evidence. The deceleration impact system has been built to analyze the mechanics of the needle and the gauge plate. Two isolated groups were designed to record the speed values under the same sample labels from real crashes. The visualization platform was built for the first group to collect needle marks. The second group recorded the speed values by other methods. The collision deceleration, the gauge plate materials, and the collision directions determine the forming of the needle marks. There were eight positive results from the 23 effective samples (12 frontal, four side, and seven rear), with discernible tip and/or middle marks on gauge plates. Multiple marks have been distinguished effectively. The low- and high-speed impacts have no obvious differences for real needle marks. It is more accurate for frontal impacts.     

An Unusual Case of Predation: Dog Pack or Cougar Attack?

Injuries produced by animals are capable of leaving severe patterns and in some cases may result in the death of the attacked individual. Law enforcement authorities may come to erroneous conclusions about the source of the bites based on their awareness of animals present and similarities of the injuries to the untrained eye, with dreadful consequences. Expertise of a carnivore biologist and an odontologist that indentifies the particularities of bite marks may be useful for identifying the attacking species. We present the investigation of a fatal dog pack attack involving a 43‐year‐old man in Bell Ville (Argentina) where the evidence provided by a forensic dentist and a biologist was categorical for establishing the animal species involved. Because of the unusual characteristics of the wounds and the initial hypothesis made by local authorities of a cougar attack, habits and specific patterns of both dog pack and cougar predation on humans are discussed.     

Confocal microscopy analysis of breech face marks on fired cartridge cases from 10 consecutively manufactured pistol slides

Recent publications from the National Academy of Sciences have called for additional foundational research in the field of firearm and toolmark analysis. We examined test fires from 10 pistol slides with consecutively manufactured breech faces. A total of nine test fires from each pistol slide, for a total of 90 test fired cartridge cases, were compared using confocal microscopy combined with three-dimensional cross-correlation analysis algorithms. A total of 8010 comparisons were performed (720 matches and 7290 nonmatches). The average score for matches was 0.82 with a standard deviation of 0.06. The average score for nonmatches was 0.20 with a standard deviation of 0.03. Additionally, subclass toolmarks were observed on the breech faces, but the presence of subclass was not detected in the correlation analysis. There was no overlap of scores between matching and nonmatching test fires. This provides objective data that support the AFTE (Association of Firearms and Tool Mark Examiners) theory of identification.     

Computer‐aided Dental Identification: An Objective Method for Assessment of Radiographic Image Similarity*

Abstract: A pilot study evaluated a computer‐based method for comparing digital dental images, utilizing a registration algorithm to correct for variations in projection geometry between images prior to a subtraction analysis. A numerical assessment of similarity was generated for pairs of images. Using well‐controlled laboratory settings, the method was evaluated as to its ability to identify the correct specimen with positive results. A subsequent clinical study examined longitudinal radiographic examinations of selected anatomical areas on 47 patients, analyzing the computer‐based method in making the correct identification based upon a threshold level of similarity. The results showed that at a threshold of 0.855, there were two false negative and two false positive identifications out of 957 analyses. Based on these initial findings, 25 dental records having two sets of full mouth series of radiographs were selected. The radiographs were digitized and grouped into six anatomical regions. The more recent set of films served as postmortem images. Each postmortem image was analyzed against all other images within the region. Images were registered to correct for differences in projection geometry prior to analysis. An area of interest was selected to assess image similarity. Analysis of variance was used to determine that there was a significant difference between images from the same individual and those from different individuals. Results showed that the threshold level of concordance will vary with the anatomical region of the mouth examined. This method may provide the most objective and reliable method for postmortem dental identification using intra‐oral images.     

Homicidal Snake Bite in Children

Snake bites are common in many regions of the world. Snake envenomation is relatively uncommon in Egypt; such unfortunate events usually attract much publicity. Snake bite is almost only accidental, occurring in urban areas and desert. Few cases were reported to commit suicide by snake. Homicidal snake poisoning is so rare. It was known in ancient world by executing capital punishment by throwing the victim into a pit full of snakes. Another way was to ask the victim to put his hand inside a small basket harboring a deadly snake. Killing a victim by direct snake bite is so rare. There was one reported case where an old couple was killed by snake bite. Here is the first reported case of killing three children by snake bite. It appeared that the diagnosis of such cases is so difficult and depended mainly on the circumstantial evidences.     

Morphometric analysis of third molar root development by an experimental method using digital orthopantomographs

Abstract:  The aim of the study was to conduct a morphometric analysis of the root development of the third molar, with the purpose of overcoming the limits of an exclusively morphological analysis. The study was divided in two phases. The first one was the verification of the existence of a constant crown-root (C/R) ratio on a sample of 100 third molars, characterized by a complete root development. The value obtained was used in the second phase to predict the final root's length, knowing the crown height. So we have calculated, on a sample of 322 third molars with developing roots, the final ratios between incomplete roots and complete roots. Statistical analysis was then made with 90%, 95%, and 99% confidence intervals. The results showed a significant difference according to the age of the subject and the limit values, lower and upper, for subjects aged 16 and 17 years versus those aged 18 and 19. For each analysis, the width of the class of tolerance and other statistical indicators were calculated. The results we obtained support the advantage of a morphometric study as opposed to an exclusively morphological study, but all the techniques used to determine the age of a living subject can provide only indications of the biological age, but no certainties as regards chronological age.     

Lessons learned from large-scale comparative dental analysis following the South Asian tsunami of 2004

The aim of this study was to examine the quality of the ante-(AM) and postmortem (PM) dental data that were submitted for entry into the PLASS data system in Phuket, Thailand, following the Boxing Day (December 26) Tsunami, 2004. The investigators were two forensic odontologists who were part of the New Zealand Disaster Victim Identification team that worked at Wat Yang Yao morgue and at the Information Management Center in Phuket. Our findings underline the usefulness of dental data in human identification, but point to a number of significant sources of error. Of the 78 PM records received, only 68% of radiographs and 49% of photos confirmed the accompanying dental charting. This underlines the value, particularly of photographs of the dental arches, in quality control. It also points to a large error component, which may have been due to inexperience of the operators, fatigue, poor conditions in the temporary morgue, or the problem of tooth-colored fillings. Of the 106 AM records received, 62% were of unacceptable quality and 64% were either not accompanied by radiographs or had poor quality radiographs. These results indicate that AM data collection ideally needs to be collated and checked by a forensically trained dentist(s) in the country of origin.     

Radiation safety for the NOMAD portable X-ray system in a temporary morgue setting

The purpose of this study was to determine the radiation levels resulting from leakage and scatter encountered by the forensic dental personnel using the Nomad at St. Gabriel, LA, following Hurricane Katrina. Using a Keithley Radiation Survey Meter and Lucite head phantom, radiation levels were measured at various distances and angles from the Nomad corresponding to the positions occupied by the dental personnel at St. Gabriel. The measurements were used to approximate the maximum total radiation dose from the Nomad to each team member for a 2- and a 4-week deployment. The results show that the maximum scatter radiation dose to any team member was 4.4 microR per X-ray or 0.253 millisieverts (mSv) for a 2-week deployment and 0.506 mSv for a 4-week deployment. Therefore, the leakage and scatter radiation dose from the Nomad was insignificant compared with established radiation safety guidelines of 50 mSv per year for all team members.     

Bite marks: physical properties of ring adhesion to skin--phase 2

Abstract: Bite Marks: This study demonstrated that surface wetness was the most influential factor affecting ring adhesion to skin. Also, chemical depilatories and shaving creams were to be avoided when cleaning the skin. The second phase of this research examines the tensile stress needed to rupture the bond between TAK^® hydroplastic, three new cyanoacrylates, and pigskin with particular consideration for temperature variations. This study also considers solubility issues of different cyanoacrylates in 10% formalin. Finally, the Dorion Type V bitemark excision technique could significantly reduce the risks of tissue distortion when used in conjunction with the following methods and materials. The skin should be devoid of moisture, razor shaved, and cleaned with dishwashing detergent and 98.9% ethanol while avoiding the use of shaving creams and/or chemical depilatories where ring placement is anticipated. The use of unopened cyanoacrylate is encouraged with Permabond^® as the cyanoacrylate of choice.