Preservation of methanol in cadaveric material

Duration of preservation of methanol in cadaveric material at 20 +/- 3 degrees C during 4-13 months was studied on 19 model and expert objects of cadaveric material (muscle tissue, kidney, blood, urine). Exponential relationship between methanol concentration and duration of storage of the object was detected. Methanol concentration decreased by 30-70% during the first weeks, after which the process decelerated to 5-10% a month. Toxicologically significant concentrations of endogenous methanol were not revealed in any case.     

A method of choosing the extractant for the recovery of tin from the internal organs of a human cadaver

The analysis of naphthenic acid revealed that they may be used for tin detection in the cadaveric material due to their high complex-forming ability. Method of extraction-atomic absorption tin determination in human organs was developed on the basis of naphthenic acid use. Modified method which differs from the common one by higher sensitivity and selectivity is presented.     

The detection and separation of antiparkinson preparations in cadaveric material by gas-liquid chromatography

Method of gas-chromatographic detection of anti-parkinsonism substances (amedine, amysile, dinesine, midantane, tropazine and cyclodole) isolated from the cadaveric material is suggested. Conditions for separation of anti-parkinsonism substances when they are all present in the cadaveric material are developed.     

An evaluation of methods for isolating antiparkinson preparations from cadaveric material

Methods of amedine, amyzyl, and tropacine isolation from cadaveric material, blood and urine were developed. They ensure higher output of the given substances as compared with conventional methods of isolation of toxicologically significant substances. An extraction-photometric method was developed for estimation of amedine, amyzyl, and tropacine in the cadaveric material.     

The forensic chemical analysis of cadaveric material for the presence of drug and narcotic compounds]

The authors consider that the list of drugs and narcotics which can be reliably identified in a general forensic chemical analysis of cadaveric material (not a purposeful screening) should be changed. Isolation by acidified water, hydrochloric acid hydrolysis, and by acetonitrile should be used for isolation of drugs and narcotic compounds in forensic chemical analysis of cadaveric material.     

The determination of phoxim in cadaveric material by gas-liquid chromatography]

The developed gas-liquid chromatographic procedure for forensic chemical detection and measurement of phoxim in cadaveric material permits the detection in 25 g of an organ of 44 to 52% of added phoxim. The threshold of phoxim measurement in 100 g of the liver is 0.04 mg, the least volume of phoxim detectable being 0.02 g. The developed gas-liquid chromatographic technique should be used as an additional method together with thin-layer chromatography, for a more objective evaluation of the results of forensic chemical analysis of cadaveric material for phoxim.     

Detection of azaleptine in forensic chemical examination of cadaveric material

Azaleptine detection and quantitation in cadaveric material in forensic-chemical tests using thin-layer chromatography, UV-spectrophotometry, gas chromatography-mass spectrometry are outlined.     

The isolation of amitriptyline from cadaveric material using acetonitrile

Method of amitriptyline isolation from the cadaveric material using acetonitrile as extractant is suggested. This method makes it possible to extract in average 76.09% of substance previously added in quantity of 1 mg to 25 g of the liver. Method was successfully used in analysis of expert material.     

The use of gel chromatography for the purification of beta-adrenoblockaders isolated from cadaveric material]

Efficacy of gel chromatography for purification of extracts from cadaveric material containing beta-adrenoblockers was shown in model tests. Distribution curves and chromatographic features of beta-adrenoblockers obtained on hydrophilic and lipophilic-hydrophilic Sephadex were studied.     

The detection of novocaine and p-aminobenzoic acid in cadaveric material

Microcrystalloscopic reactions, TLC and UV spectroscopy methods for novocainum and p-aminobenzoic acid identification in medicolegal examination of the cadaveric material were developed. Sensitivity and specificity of microcrystalloscopic reactions were studied.     

Comparative study of methods of isolating ergometrine from cadaveric material

The results of comparative study using three methods of ergometrine isolation from cadaveric material with respect to Vasiljeva, Kramarenko, Stas-Otto are presented. Vasileva's method appeared to be more effective.     

Isolation of nitrobenzene from biological material

Toluene is recommended as an agent for extraction of nitrobenzene from biological material. Optimal conditions for nitrobenzene isolation from cadaveric human liver are defined and the results of isolation are quantitatively evaluated.     

Characteristics of dipyridamol isolation from biological material

Acetone is proposed as an isolating agent for extraction of dipyridamolum from biological material. Optimal conditions of isolating dipyridamolum from human cadaveric liver tissue with acetone are determined and quantitative estimation of isolation results is provided.     

Characteristics of alphacypermethrin isolation from biological material

Methanol is proposed as an isolating agent for isolation of alphacypermethrin from biological material. Optimal conditions of isolating alphacipermetrin from human cadaveric liver tissue with methanol are determined and quantitative estimation of isolation results is provided.     

Isolation of lambda-cyhalothrin from biological material

Dioxan is proposed as an isolating agent for isolating lambda-cytalotrin (LC) from biological material. Optimal conditions for isolating LC from cadaveric hepatic tissue with dioxan were determined and the results of the isolation were quantified.     

The analysis of tiapride in cadaveric material

To optimize conditions of tiapride isolation from cadaveric organs, we compared the results of conventional methods by Stas-Otto, A.A. Vasilyeva and V.F. Kramarenko which provide tiapride isolation up to 50% and a new precise and reproducible method providing 60 +/- 2% tiapride isolation. Identification of tiapride isolated from cadaveric material was made with thin-layer chromatography and high performance liquid chromatography. The latter assay employed the method of external standard. The original techniques proposed identify and measure tiapride in hepatic samples in the presence of unidentified endogenic compounds. The techniques are rapid, selective, sensitive and reproducible.     

A method for determining molybdenum in cadaveric material by using alkylphenylcyclohexylcarboxylic acid]

Alkylphenylcyclohexylcarboxylic acid was found to be an efficient extractant to extract molybdenum from the cadaveric material. Method suggested is simple enough for use and doesn't require considerable amounts of chemical reagents.     

The detection of the presence of azafen in cadaveric material

Possibility of isolating azaphen from the cadaveric material by acidified water and acetonitrile (as extractant) is shown. Isolation rate (75.6%) of azaphen (with 1 mg of preparation added to 25 g of the liver by acetonitrile is significantly higher than in isolation by acidified water (41.7%).     

Detection of acetic acid in cadavers by gas chromatography

A simple technique of sample preparation for gas chromatographic test for acetic acid in cadaveric material has been developed. The background concentration of natural content of acetates in the stomach, liver and kidneys are determined. The error of the method is no more than 10% with standard deviation +/- 0.04-0.13.     

The determination of chromium in cadaveric material by using cyclohexanecarboxylic acid

Method of chrome detection in the course of forensic chemical investigation in the cadaveric material on the basis of cyclohexan-carbonic acid use is presented. Results of experimental researches showed efficacy of method tested as compared to the common one. Method suggested can be used in laboratory conditions to extract chrome from visceral organs of human cadaver.