GoldeneyeTM 20A-M试剂盒在数据库建设中的应用

目的 研究GoldeneyeTM 20A-M试剂盒在DNA数据库建设中的应用.方法 针对血滤纸特性,优化不同条件血痕样品的取样量、扩增循环次数,缩短PCR扩增时间,以建立适合批量样品直接快速扩增检验的方法,并将其应用于1 200份数据库样品的检验中.结果 确定了批量样本检验的最适取样量和扩增循环次数,建立了快速扩增反应程序,1 200份样品的直接快速扩增检验成功率为98.4％,批量检验92份样品(1板)从取样到电泳完成只需6h.结论 GoldeneyeTM 20A-M试剂盒的应用,免去DNA提取过程,方法简单、快速、获得信息量大,适用于DNA数据库建设的需要.     

Comparison of wound morphology following gunshots by machine guns and sub-machine guns

Automatic weapons such as machine guns and submachine guns are found in the German-speaking region only in special army and police units and appear accordingly rarely in homicides, suicides and accidents. In the following, the findings in two cases of death with the use of machine and submachine guns are presented. The first case was a fatal accident during shooting on a training area (current machine gun of the German army, calibre 7.62 x 51 mm), the second case was a killing during a physical conflict (submachine gun MP 40 from World War II, calibre 9 x 19 mm). In the case with the machine gun autopsy disclosed typical entry holes corresponding to the calibre, but unusually large exit wounds with tissue bridges in the wound ground, measuring 4 x 2.5 cm in diameter. By contrast, the second case (submachine gun) showed "normal" entry and exit wounds. The differences are mainly caused by deviating ballistic data of the ammunition used. They are discussed against the background of literature on wound ballistics.     

Internal capabilities,external structural holes network positions,and knowledge creation

Social network theory suggests that firms bridging structural holes by connecting disconnected partners in a network benefit from timely access to diverse knowledge. However, the existing literature reports mixed evidence with regard to the performance implications of this view. Our study examines how a firm’s diverse knowledge base and knowledge-processing capabilities affect knowledge creation when a firm bridges multiple structural holes in an inter-firm network. The model is tested on a sample of 191 firms in high-technology industries. The dependent variable, knowledge creation, was measured by the number of patents generated. Results show that when a firm spans multiple structural holes, its diverse technological knowledge facilitates knowledge creation. However, when a firm already possesses well-developed knowledge acquisition capabilities, spanning structural holes reduces knowledge creation. This paper suggests that internal knowledge base and knowledge-processing capabilities determine the impacts of external network positions on firm knowledge creation. It provides implications on how a firm best capitalizes on external network positions.     

Machine washing or brushing of clothing and its influence on shooting distance estimation.

Experiments were conducted to assess the effect of machine washing or brushing of clothing items on Gunshot Residue (GSR) patterns (gunpowder residues, lead, and copper, deposits) around bullet entrance holes. Results show that those treatments decrease considerably the amount and density of GSR. However, for close shooting distances not all of the GSR deposits are removed. Remaining patterns may be visualized by specific color reactions and used for shooting distance estimation.     

Performance Testing of a Semi-Automatic Card Punch System,Using Direct STR Profiling of DNA from Blood Samples on FTA™ Cards

The 1.2 mm Electric Coring Tool (e-Core™) was developed to increase the throughput of FTA^™ sample collection cards used during forensic workflows and is similar to a 1.2 mm Harris manual micro-punch for sampling dried blood spots. Direct short tandem repeat (STR) DNA profiling was used to compare samples taken by the e-Core tool with those taken by the manual micro-punch. The performance of the e-Core device was evaluated using a commercially available PowerPlex™ 18D STR System. In addition, an analysis was performed that investigated the potential carryover of DNA via the e-Core punch from one FTA disc to another. This contamination study was carried out using Applied Biosystems AmpflSTR™ Identifiler™ Direct PCR Amplification kits. The e-Core instrument does not contaminate FTA discs when a cleaning punch is used following excision of discs containing samples and generates STR profiles that are comparable to those generated by the manual micro-punch.     

Ballpoint pen inks: the quantitative analysis of ink solvents on paper by solid-phase microextraction

We wish to describe further developments to a method previously reported on the detection of 2-phenoxyethanol in ink. The solid-phase microextraction (SPME) sampling technique, together with gas chromatography-mass spectrometry (GC-MS), has been used to quantify solvents in writing ink. In conventional approaches, the analysis of ink on documents requires some degree of destructive sampling. The methods commonly used remove ink samples from paper using a scalpel or a paper punch. To avoid document destruction, a sampling cell was constructed that allows solvents to be adsorbed directly onto the SPME fiber from the headspace above the document surface. Analytes (ink volatiles) are then desorbed from the SPME fiber on a gas chromatograph equipped with a mass selective detector (GC-MSD). With this method, it was possible to detect the presence of ink solvents on documents for a period lasting up to c. 2 years.     

Acute Fatal Coronary Artery Dissection Following Exercise‐related Blunt Chest Trauma

Coronary artery injury such as acute coronary dissection is an uncommon and potentially life‐threatening complication after blunt chest trauma. The authors report an unusual autopsy case of a 43‐year‐old healthy man who suddenly collapsed after receiving a punch to the chest during the practice of kung fu. The occurrence of the punch was supported by the presence of one recent contusion on the left lateral chest area at the external examination and by areas of hemorrhage next to the left lateral intercostal spaces at the internal examination. The histological examination revealed the presence of an acute dissection of the proximal segment of the left anterior descending coronary artery. Only few cases of coronary artery dissection have been reported due to trauma during sports activities such as rugby and soccer games, but never during the practice of martial arts, sports usually considered as safe and responsible for only minor trauma.     

Transfer and Distribution of Gunshot Residue through Glass Windows

Research into the deposition patterns of primer gunshot residue (GSR) beyond a primary target was previously nonexistent. This study aimed to determine the deposition patterns of GSR once a bullet passed through an initial target and continued on its path into additional targets. Multiple repetitions were performed to assess the GSR deposition patterns after a bullet was shot through a closed window into either a dummy or a wall within an enclosed room. Samples were taken from both the primary and secondary target holes as well as from nontarget areas. Significant amounts of GSR were found on all samples. The results show that GSR continues to be deposited along the path of the bullet after passing through a primary glass target. These findings reiterate the lack of probative value in collecting GSR samples from gunshot victims even if they are in an enclosed area separate from the shooter.     

Author gender identification from text

Text is still the most prevalent Internet media type. Examples of this include popular social networking applications such as Twitter, Craigslist, Facebook, etc. Other web applications such as e-mail, blog, chat rooms, etc. are also mostly text based. A question we address in this paper that deals with text based Internet forensics is the following: given a short text document, can we identify if the author is a man or a woman? This question is motivated by recent events where people faked their gender on the Internet. Note that this is different from the authorship attribution problem.In this paper we investigate author gender identification for short length, multi-genre, content-free text, such as the ones found in many Internet applications. Fundamental questions we ask are: do men and women inherently use different classes of language styles? If this is true, what are good linguistic features that indicate gender? Based on research in human psychology, we propose 545 psycho-linguistic and gender-preferential cues along with stylometric features to build the feature space for this identification problem. Note that identifying the correct set of features that indicate gender is an open research problem. Three machine learning algorithms (support vector machine, Bayesian logistic regression and AdaBoost decision tree) are then designed for gender identification based on the proposed features. Extensive experiments on large text corpora (Reuters Corpus Volume 1 newsgroup data and Enron e-mail data) indicate an accuracy up to 85.1% in identifying the gender. Experiments also indicate that function words, word-based features and structural features are significant gender discriminators.     

Accidental Ligature Strangulation by an Ironing Machine: An Unusual Case*

Abstract: In this paper, we present a case of a 53‐year‐old woman who had her headscarf catch on the cylinder ironing machine in the laundry of the hospital where she worked. The hospital workers found the woman dead with her head stuck to the ironing machine. After the death scene investigation and autopsy were completed, it was determined that the death occurred as a result of accidental ligature strangulation. Accidental ligature strangulation in which an article of clothing is caught in such an electrical machine and strangles the wearer is very rare. This case highlights the fact that these kinds of machines can be hazardous to work around and that increased safety measures should be taken to insure worker safety; additionally, the people who use these machines should be educated on the potential hazards.     

变动装订痕迹的特征及检验方法

对机制、半机制和手工装订的原始痕迹特征和变动痕迹特征进行叙述描绘,并着重介绍变动装订痕迹的检验方法.     

From alcohol to liquid ecstasy (GHB)--a survey of old and modern knockout agents. Part 1: historic and classic knockout agents

Alcohol has been the most important knockout drug in history and literature and continues to play an essential role up to now. Blunt force to the head in the form of a knockout punch is another mechanism leading to a transient loss of consciousness. Diethyl ether and chloroform are among the classical knockout substances. Although they have meanwhile been replaced by modern sedatives and hypnotics, their use is still observed in isolated cases.     

利用SEM/EDS区别小口径手枪及六四式手枪在纺织品上的枪击孔

利用扫描电子显微镜和能谱仪（SEM/EDS）对小口径手枪、六四式手枪在纺织品上枪击孔洞处的附着物进行了观察分析,较好地解决了判定是小口径手枪还是六四式手枪射击问题。     

Systematic approach for investigating basic drugs by paper- and thin-layer chromatography (author's transl)]

The systematic approach described for investigating basic (pharmaceutical) drugs with the aid of paper and thin-layer chromatography relies on the principle that the positions of the spots created by a certain substance on various chromatograms in relation to 5 to 7 test substances and developed together with the sample are indicated by a digit code. With a pre-determined sequence of application of the solvents, code numbers are formed by writing down these digits (supplemented by indices), which are characteristic of the components of the mixture. The code numbers are in all cases clearly distinctive even in the case of a large number of different substances (e.g. 300) if the number of appropriately chosen developing solutions is increased to 6. Evaluation of the code numbers can be carried out with tables or with the aid of punch cards.     

Can a machine be used to replicate the biomechanics of human stabbing performance?

A human performance trial was conducted where the stabbing actions of 40 participants were investigated. A range of factors were assessed such as the action used when the blade shape and familiarity of the knife changed. A qualitative evaluation of these factors was presented in an earlier paper to precede the analysis of quantitative kinematic data in this paper. The data from participants was then compared to that obtained from a stabbing machine.A motion analysis system was used to measure parameters such as the joint angular displacement and absolute linear velocity at impact. Motion data was collected using a 12-camera Vicon MX motion analysis system. Results indicated that the mean absolute linear velocity of overarm stabbing actions was consistently higher than the underarm actions.Forensic textile damage examinations often involve testing of edged weapons by manually performing stabbing actions. An underlying fundamental limitation of manual simulation experiments is the natural variation inherent in human performance. Sufficient control measures can be implemented to successfully use manual simulation experiments in forensic testing and therefore this is the accepted practice. However, performance can be improved using a stabbing machine. A custom-built instrumental stabbing device has been shown to operate within the same parameters of the human participants and is therefore suitable for use in casework.     

试论证券市场中的非法承销证券犯罪

本文突破现有刑事立法的框架结构 ,大胆探索新型证券犯罪的立法空间 ,对证券市场非法承销刑事犯罪化问题进行了探讨 ,以弥补《证券法》与《刑法》在欺诈客户行为刑事责任问题上的漏洞。     

The asymmetrical remodelling of two neurosurgical burr holes: a case study

A human skull identified as that of a 16-year-old female who had been reported missing about 3 years before the discovery of the specimen displayed evidence for the asymmetrical remodelling of bilateral neurosurgical burr holes. While one of the holes retained its original size and shape, gross evidence for the contralateral lesion had virtually disappeared. Initially, the open lesion was mistakenly suspected to be a recent bullet hole.     

Forensic-medical significance of the characteristics of gunshot perforating fractures of the skull bones

The study was made of gunshot perforating fractures of calvarial bones in cases of lethal perforating head wounds in long distance shots from a 9-mm Makarov gun. Morphological characteristics of inlet and outlet perforating fractures and bone fragments were found to differ. Gunshot fractures in calvarial bones inflicted by bullets from a 9-mm Makarov gun were studied in experiments with different kinetic energy. In low kinetic energy, bullets make oval holes on the outer table of the skull and round holes on the inner table with radial prominences on the walls of bullet channels, large bone fragments; in moderate kinetic energy--make round holes in the outer table and oval on the inner table with skew prominences on the walls of the outlet part of the channels, large and middle-sized flat bone fragments; in high kinetic energy injuries on both tables were round, prominences were cross, bone fragments were flat, small and middle-sized.     

A Safer Method for Body Restoration Following Autopsy

Restoration of autopsy incisions can result in inadvertent needle stick injury to the prosector, which can be difficult to prevent even with the use of personal protective equipment such as Kevlar cut resistant gloves. We present a new technique for closure of autopsy incisions using a commonly available commercial hardware tool, a hole punch with a lever enhanced action, combined with blunt probe sewing, which results in an esthetic and leak‐proof means of restoring cadavers. This technique is especially useful in cases which may pose blood‐borne infection risks to the prosector.