Comparative study of methods of isolating ergometrine from cadaveric material

The results of comparative study using three methods of ergometrine isolation from cadaveric material with respect to Vasiljeva, Kramarenko, Stas-Otto are presented. Vasileva's method appeared to be more effective.     

An evaluation of methods for isolating antiparkinson preparations from cadaveric material

Methods of amedine, amyzyl, and tropacine isolation from cadaveric material, blood and urine were developed. They ensure higher output of the given substances as compared with conventional methods of isolation of toxicologically significant substances. An extraction-photometric method was developed for estimation of amedine, amyzyl, and tropacine in the cadaveric material.     

Isolation of eugenol from biological material

The optimal conditions for isolation of eugenol from biological material by ethyl acetate were determined. Possible purification of the compound from coextractive substances of biomaterial on the colon with silica gel L 40/100 micro is demonstrated. Methods of thin-layer and high-yield liquid chromatography for identification and quantitative determination of eugenol are proposed.     

Isolation of lambda-cyhalothrin from biological material

Dioxan is proposed as an isolating agent for isolating lambda-cytalotrin (LC) from biological material. Optimal conditions for isolating LC from cadaveric hepatic tissue with dioxan were determined and the results of the isolation were quantified.     

Isolation of nitrobenzene from biological material

Toluene is recommended as an agent for extraction of nitrobenzene from biological material. Optimal conditions for nitrobenzene isolation from cadaveric human liver are defined and the results of isolation are quantitatively evaluated.     

Characteristics of dipyridamol isolation from biological material

Acetone is proposed as an isolating agent for extraction of dipyridamolum from biological material. Optimal conditions of isolating dipyridamolum from human cadaveric liver tissue with acetone are determined and quantitative estimation of isolation results is provided.     

Characteristics of alphacypermethrin isolation from biological material

Methanol is proposed as an isolating agent for isolation of alphacypermethrin from biological material. Optimal conditions of isolating alphacipermetrin from human cadaveric liver tissue with methanol are determined and quantitative estimation of isolation results is provided.     

Purification of extracts from biological material using aluminum oxide

An effective, rapid, and safe operation is suggested for purification of extracts from biological material from co-extractive substances during testing for barbiturates, benzophenones, and nichlofolane. The purification is carried out on a column packed with aluminum oxide.     

Comparison of four DNA extraction methods to extract DNA from cigarette butts collected in Lebanon

Cigarette butts collected from crime scenes represent valuable sources of DNA. However the extraction of the genetic material may deem challenging especially when different contaminants may compromise the integrity, quality, and quantity of DNA obtained. This study aims at comparing four extraction methods (Chelex-100, soaking + Chelex-100, Chelex-100?+?PK, and DNA IQ? System) with the intention of identifying the one with maximal recovery rate and profiling success. DNA was extracted using aforementioned four methods from 70 cigarette butts collected from sites across Lebanon. DNA was quantified by qPCR using TaqMan Quantifiler Kit on an Applied Biosystems 7300 SDS instrument and genotypes were obtained using the PowerPlex® 21 kit on an Applied Biosystems 3130 Genetic Analyser. The findings of this work showed that DNA extraction with Chelex-100?+?PK is preferred to the other three methods when seeking both, a high yield and the generation of maximal numbers of full profiles. The Chelex-100?+?PK method is simple, cost effective, and therefore suitable for routine cigarette butts case studies.     

Peculiarities of detection and evaluation of the persistence of tetraethyltiuram disulfide in the biological material

Optimal conditions for the identification and quantitative determination of tetraethyltiuram disulfide in "fresh" and putrefactive tissues of cadaverous liver are described for the purpose of TLC, HPLC, and IK-spectrophotometry following extraction of the compound of interest with ethyl acetate and its purification on a silicagel L column, 40/100 mcm. The persistence of tetraethyltiuram disulfide in the cadaverous tissues was evaluated. It was shown that the period during which tetraethyltiuram disulfide can be detected in the autopsied tissues decreases from 203 to 28 days with a rise in temperature from -15 degrees C to +36 degrees C.     

DNA profiles from biological trace material contained in underwear and toothbrushes

There are special cases where it is extremely difficult to find a close relative for obtaining reference samples, such as in adoption cases or foreigners without relatives in the country. In these cases, it is possible to use personal objects from the missing person. We developed a protocol for obtaining DNA profiles from toothbrushes and underwear and evaluated two DNA extraction methods (Silica and magnetic particles), comparing the DNA quantity and the quality of the obtained profiles. Results showed that for underwear, silica in suspension recovered higher DNA quantity but magnetic particles (PrepFiler®) generated more complete profiles. For toothbrushes PrepFiler® recovered a higher DNA quantity and generated more complete DNA profiles. Statistical analyses showed there were no significant differences between both methods. Higher DNA quantities were recovered from underwear samples, however toothbrushes generated higher quality in DNA profiles, without obtaining mixed profiles, concluding that toothbrushes are a better item than underwear. Considering that silica in suspension method is three-times cheaper than PrepFiler®, we propose it is possible to successfully obtain DNA profiles from personal objects using a low-cost DNA extract method.