Allele frequency distribution for 21 autosomal STR loci in Nepal

The allele frequency distributions of 21 autosomal loci contained in the AmpFlSTR Identifiler, the Powerplex 16 and the FFFL multiplex PCR kits, was studied in 953 unrelated individuals from Nepal. Several new alleles (i.e. not yet reported in the NIST Short Tandem Repeat DNA Internet DataBase [http://www.cstl.nist.gov/biotech/strbase/]) have been detected in the process.     

The Upper Silesia (Poland) population and forensic usefulness of 15 autosomal STR loci

Allele frequencies, forensic parameters for the 15 STR loci in the AmpFlSTR^® Identifiler Kit (Applied Biosystems), D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D19S433, VWA, TPOX, D18S51, D5S818 and FGA were determined in a sample of 150 unrelated dead and alive adults from the Upper Silesia region (Poland). The values of heterozygosity (Ht), polymorphic information content (PIC), power of discrimination (PD), matching probability (PM), mean exclusion chance (MEC) and mean exclusion probability (MEP) were calculated. Possible divergence from HWE was determined. Comparison of allele frequencies for examined STR loci between the Upper Silesia population and other Polish populations was carried out.     

Allele frequency distribution of two X-chromosomal STR loci in Han population in China

POPULATION: 127 unrelated female and 118 unrelated male volunteer donors, Southwest China.     

Effect of blood stained soils and time period on DNA and allele drop out using Promega 16 Powerplex kit

Blood stained soils may be of great interest in forensic incidents. Amplification of DNA from soil is often inhibited by co-purified contaminants. Different soils types from Pakistan and Turkey were stained with blood and samples were collected systematically after specified intervals. Rapid, inexpensive, large-scale DNA extraction method involving minimal purification was developed. DNA was quantized using Spectrophotometer and Fluorometer and was confirmed by Agarose Gel Electrophoresis. DNA extracted from different soils in different periods showed a remarkable decrease in yield as well as degradation in every extraction. PCR amplification was performed using various DNA targets present in Promega 16 Powerplex^® System kit. Amplification could not carry out in all loci especially in degraded samples taken after 20 days. Allele n locus drop out was noticed which shows that DNA was degraded. For some loci more than 2 alleles were also noticed showing contamination while working with the blood stained soils.     

Allele frequencies for 15 autosomal STR loci and admixture estimates in Puerto Rican Americans

Allelic frequencies of 15 short tandem repeats (STR) markers (CSF1PO, FGA, THO1, TPOX, VWA, D3S11358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, D19S433 and D2S1338) were determined using the AmpFl STR Identifiler PCR Amplification Kit in Puerto Rican American individuals (N=205) from Massachusetts. The FGA, D18S51 and D2S1338 loci had a high power of discrimination (PD) with values of 0.967, 0.965 and 0.961, respectively. Significant deviations from the Hardy-Weinberg (HW) equilibrium were not detected. An important genetic contribution of Caucasian European (76.4%) was detected in Puerto Rican Americans. However, comparative analysis between Puerto Rican American and other neighboring populations from United States mainly with African and Caucasian Americans, revealed significant differences in the distribution of STR markers. Our results are important for future comparative genetic studies of different American ethnic groups, in particular a cultural group called Hispanic-Americans and should be helpful for forensic and paternity testing.     

中国人群亲权鉴定常用STR基因座平均突变率的估计

目的对亲权鉴定中常用STR基因座在中国人群中的平均突变率进行估计,并与美国人群中的相应数据进行比较。方法突变数据来自按拟定标准筛选获得的15篇国内文献及本中心数据。对中国不同地区同一STR基因座突变率无显著差异的突变数据进行合并,计算中国人群该STR基因座的平均突变率,并采用Poisson分布的近似正态分布法计算该STR基因座平均突变率的95%可信区间。对中国人群和美国人群中常用STR基因座平均突变率的95%可信区间进行比较。结果 Identifiler和PowerPlex 16两个系统所包含的17个亲权鉴定常用STR基因座在中国不同地区人群中的突变率无显著差异,合并计算其平均突变率介于0.0120%～0.2078%,不同STR基因座的平均突变率差异显著（P〈0.0001）。这17个亲权鉴定常用STR基因座在中国人群中的累积突变率达到1.9836%。结论本研究通过文献分析获得中国大样本人群的FGA等17个STR基因座突变率数据,对于疑似突变案例的累积亲权指数计算具有借鉴价值。     

Analysis of 15 autosomal STR loci in the population of the State of Acre,Brazilian Amazonia

Acre was the last state of Brazil to be inhabited by non-indigenous individuals. The aim of this study was to calculate the allele frequencies of 15 STR loci in 503 unrelated individuals living in Acre, as well as to estimate statistical parameters of forensic interest. The Hardy–Weinberg equilibrium test performed in the overall sample, as well as population comparisons between sub-samples from the five regions in Acre did not reveal the presence of population substructure. This is the first report of STR data in this population and the results showed that a single database is suitable for all the regions analyzed.