Morphologic patterns of lip prints in a portuguese population: a preliminary analysis

Lip prints are thought to have the ability to distinguish individuals and, hence, have a potential use in human identification purposes. However, questions remain regarding their utility for sex determination. This study aimed to classify lip prints for different individuals in a Portuguese population and to determine whether sex differences exist. Lip prints of 25 females and 25 males were obtained using dark-colored lipstick and cellophane tape. Lip prints were analyzed using a magnifying lens and classified according to the Suzuki and Tsuchihashi classification. A Type II pattern was found to be most common. A comparison of lip-print patterns between males and females showed results with a statistically significant difference: Type III pattern was most common in males, and a Type II pattern in females. This study corroborates the hypothesis that lip prints are able to distinguish individuals and may be useful in sex determination.     

Evaluation of Lip Prints on Different Supports Using a Batch Image Processing Algorithm and Image Superimposition

This study aimed to develop and to assess an algorithm to facilitate lip print visualization, and to digitally analyze lip prints on different supports, by superimposition. It also aimed to classify lip prints according to sex. A batch image processing algorithm was developed, which facilitated the identification and extraction of information about lip grooves. However, it performed better for lip print images with a uniform background. Paper and glass slab allowed more correct identifications than glass and the both sides of compact disks. There was no significant difference between the type of support and the amount of matching structures located in the middle area of the lower lip. There was no evidence of association between types of lip grooves and sex. Lip groove patterns of type III and type I were the most common for both sexes. The development of systems for lip print analysis is necessary, mainly concerning digital methods.     

Persistent lipsticks and their lip prints: new hidden evidence at the crime scene

Latent lip print test results produced by permanent lipsticks are presented in this work. This cosmetic product, contrary to conventional lipsticks, does not leave visible prints and can thus be overlooked at the crime scene. As print vehicles the study used ceramics, glass, cotton fabric and paper. Lip prints were left to dwell for different periods and were later developed using aluminum powder, cobalt oxide powder and magnetic powder. The results show that identifiable lip prints can be obtained up to 30 days after being produced.     

中国汉族唇纹研究(Ⅱ)——稳定性及分型

本文对部分中国汉族64名(男36名,女28名)个体的唇纹进行了追踪调查(间隔4年零5个月)。过对所得材料的研究,探讨了唇纹特征随年龄增加而发生变化的规律,并根据唇纹特征提出了新通的分类方法。     

Preliminary study of post mortem identification using lip prints

Identification using lip prints was first performed in the 1950s and was the subject of much research in the 1960s and 70s, leading to the acceptance of this technique as evidence in the criminal justice system. Previous research has focused on identifying lip print types or on methods of obtaining hidden lip prints left at the crime scene. The present study aimed to clarify characteristics of lip prints from cadavers with various causes of death (including drowning and hanging) and to determine the effects of fixation on post mortem changes in lip impressions.     

Fingerprint Ridge Density as a Potential Forensic Anthropological Tool for Sex Identification

In cases of partial or poor print recovery and lack of database/suspect print, fingerprint evidence is generally neglected. In light of such constraints, this study was designed to examine whether ridge density can aid in narrowing down the investigation for sex identification. The study was conducted on the right‐hand index digit of 245 males and 246 females belonging to the Punjabis of Delhi region. Five ridge density count areas, namely upper radial, radial, ulnar, upper ulnar, and proximal, were selected and designated. Probability of sex origin was calculated, and stepwise discriminant function analysis was performed to determine the discriminating ability of the selected areas. Females were observed with a significantly higher ridge density than males in all the five areas. Discriminant function analysis and logistic regression exhibited 96.8% and 97.4% accuracy, respectively, in sex identification. Hence, fingerprint ridge density is a potential tool for sex identification, even from partial prints.     

Distortion in Fingerprints: A Statistical Investigation using Shape Measurement Tools

Friction ridge impression appearance can be affected due to the type of surface touched and pressure exerted during deposition. Understanding the magnitude of alterations, regions affected, and systematic/detectable changes occurring would provide useful information. Geometric morphometric techniques were used to statistically characterize these changes. One hundred and fourteen prints were obtained from a single volunteer and impressed with heavy, normal, and light pressure on computer paper, soft gloss paper, 10‐print card stock, and retabs. Six hundred prints from 10 volunteers were rolled with heavy, normal, and light pressure on soft gloss paper and 10‐print card stock. Results indicate that while different substrates/pressure levels produced small systematic changes in fingerprints, the changes were small in magnitude: roughly the width of one ridge. There were no detectable changes in the degree of random variability of prints associated with either pressure or substrate. In conclusion, the prints transferred reliably regardless of pressure or substrate     

Distribution of the Minutiae in Hypothenar Palm Prints in Slovak Adults: Indications for Personal Identification

Minutiae are small distinguishing features found along every ridge flow, which make each friction ridge print unique. The most common friction ridge prints found at the crime scene are fingerprints; therefore, the most of the minutiae studies are focused exactly on this kind of prints. The authors believe that further examination and enlargement of the palm print database could result in better use of the palm prints for personal identification. We analyzed a total of 160 palm prints from 40 females and 40 males aged between 18 and 70 years from Slovakia. For the evaluation of the minutiae, the area of the hypothenar had to be marked out. The classification of the minutiae used for this study was based on a modified version of the classification system using the total of 13 types of minutiae. The frequency of every minutiae type was calculated and, using the chi-square test with Yates's correction, bilateral and sex differences were assessed. The relationship between the different types of minutiae was examined with Pearson's correlation test. During the initial phases of the identification process, the focus should be on the least common types of minutiae (Y or M and return), which were found not to correlate; thus, their mutual occurrence is random (e.g., overlap—Y or M, crossbar—return, or Y or M—dock). The results of the present study show which specific minutiae types are the most suitable for personal identification. These findings may be beneficial in more effective outcome of the identification process.     

A Comparison of Palmar Dermatoglyphics in Two Ethnic Indian Populations of North Bengal,India*

Abstract: Dermatoglyphic print comparisons can be utilized to establish personal identification in forensic cases. The northern part of the state of West Bengal, India, is the home to many ethnic populations. Two such populations are the Rajbanshi and the Mech. Palm prints were collected from 192 adult Rajbanshi (105 men and 87 women) and 100 adult Mech (50 men and 50 women) individuals for print comparison using the standard ink and roll print method. The dermatoglyphic variables studied were mainline formulae, termination of mainline, positional variation of axial triradii, and true pattern of hypothenar and thenar configuration area. There were differences between the Rajbanshi and Mech individuals with respect to these dermatoglyphic variables. The uses of these variables appear to be limited only to ethnic identification, not personal identification. The present investigation further highlights the racial affinity, sex, and bilateral differences among Rajbanshi individuals using dermatoglyphic palmar variables.     

Criminalystic: effectiveness of lysochromes on the developing of invisible lipstick-contaminated lipmarks on human skin. A preliminary study

Latent prints are an important evidence for identification. Nowadays, the technical means, the implementation of image processing techniques and the use of database makes it possible to detect and get information from some prints that seem to be useless at first sight. On the other hand, the possibility of using the print as a DNA source has to be considered, so as to double its identifying value. Human skin is a particularly difficult surface for developing this kind of evidences. Although different methods for locating and developing latent fingerprints on the skin have been already described, it has not been found any method, at the revised bibliography, to obtain and develop invisible lipmarks, that is, lipmarks from protective lipstick, or permanent or long-lasting lipstick. The aim of the work that follows is to determine the effectiveness of several reagents for developing invisible lipmarks on the corpses' skin. Preliminary results show that, under the described experimental conditions, the reagents used, Sudan III, Oil Red O and Sudan Black, are effective for obtaining recent latent lip prints on corpse's skin.     

Multi-Rater Agreement Using the Adapted Fracture Healing Scale (AFHS) for the Assessment of Tubular Bones on Conventional Radiographs: Preliminary Study*

Better understanding of the timing of fracture healing may help in cases of interpersonal violence but also of personal identification. The intra- and inter-rater agreement for the adapted fracture healing scale (AFHS) assessing the post-traumatic time interval on radiographs were tested. This is a preliminary study, providing essential information on method reliability for upcoming studies using the AFHS. Five raters (two radiologists, a forensic pathologist, an orthopedist, and an anthropologist) were presented with a test in three parts consisting of 85 radiographs (from 30 adults) of fractures of tubular bones in different stages of healing purposefully selected from more than 1500 radiographs. The raters were firstly asked to assess 15 features describing fracture healing as present, absent, or not assessable. Thereafter, the raters were asked to choose from the AFHS a single-stage best representing the observed healing pattern. The intra- and inter-rater agreement were assessed using single-rating, absolute agreement, two-way mixed-effects intra-class correlation (ICC) coefficients. The intra-rater ICC of radiologist 1 ranged from 0.80 to 0.94. The radiologists’ inter-rater ICC ranged from 0.68 to 0.74, while it ranged from −0.01 to 0.90 for the other raters. The good to excellent ICC among the radiologists and forensic anthropologist provides good foundation for the use of the AFHS in forensic cases of trauma dating. The poor to good results for the other physicians indicate that using the AFHS requires training in skeletal anatomy and radiology.     

Luminous lip-prints as criminal evidence

Luminescence is specially a useful property for the search of invisible evidences at the scene of a crime. In the latent fingerprints particular case, there are at one's disposal fluorescent reagents for their localization. The study of latent lip prints (that is lip prints from protective lipstick, or permanent or long-lasting lipstick that do not leave any visible marks) is more recent than fingerprints study. Because of the different composition of both types of prints, different reagents have been tried out on their developing. Although, lysochromes are particularly useful reagents to obtain latent lip prints, it may occur on coloured or multicoloured surfaces, the developing is not perceived due to contrast problems between the reagent and the surface where the print is searched. Again, luminescence offers the possibility to solve this problem. Nile Red is being studied as a potential developer for latent lip prints. The results on very old prints (over 1year) indicate that this reagent is highly efficient to get latent lip prints.     

Is re-creation of human identity possible using tooth prints? An experimental study to aid in identification

Enamel in teeth, as documented is the hardest substance in the entire human body. Tooth prints are the enamel rod end patterns on the tooth surface. Could this fact be a boon in utilizing it for an individual's identification in the hands of forensic experts? A study has been attempted towards the same. These tooth prints were recorded for 60 extracted tooth specimens after acid etching using a cellophane tape. Subsequently a digital image of the print was obtained which was subjected to biometric conversion using Verifinger standard SDK version 6.0 software followed by the use of Automated Fingerprint Identification System (AFIS) software for comparison of the tooth prints. In results we observed that tooth prints were composed of varied patterns and sub-patterns. A comparison was made between the tooth prints of different and the same individual and also between different classes of teeth (incisors, canines, premolars and molars). None of the patterns exhibited intra- and inter-individual similarity. Nor did any particular class of the tooth could be preferentially used over the other for an individual identification. This field demands a need for further exploration towards the use of tooth prints for establishing an individual's identity.     

Automated Mapping of Explosives Particles in Composition C‐4 Fingerprints*†‡

Abstract: A method is described to perform automated mapping of hexahydro‐1,3,5‐trinitro‐1,3,5‐triazine (RDX) particles in C‐4 fingerprints. The method employs polarized light microscopy and image analysis to map the entire fingerprint and the distribution of RDX particles. This method can be used to evaluate a large number of fingerprints to aid in the development of threat libraries that can be used to determine performance requirements of explosive trace detectors. A series of 50 C‐4 fingerprints were characterized, and results show that the number of particles varies significantly from print to print, and within a print. The particle size distributions can be used to estimate the mass of RDX in the fingerprint. These estimates were found to be within ±26% relative of the results obtained from dissolution gas chromatography/μ‐electron capture detection for four of six prints, which is quite encouraging for a particle counting approach. By evaluating the average mass and frequency of particles with respect to size for this series of fingerprints, we conclude that particles 10–20 μm in diameter could be targeted to improve detection of traces of C‐4 explosives.     

A comprehensive ATR-FTIR spectroscopic analysis for the identification and differentiation of lip balms

Lip balm may be encountered as physical evidence in cases involving sexual assaults, homicides, and kidnappings. Lip balm can be used as corroborative evidence by providing a potential link between the victim, accused, and the crime scene. For lip balms to be used as evidence, it is important to understand the diversity and their aging process under different conditions. Therefore, in this study, ATR-FTIR spectroscopy in conjunction with chemometric tools such as principal component analysis (PCA) and linear discriminant analysis (LDA) has been used for the objective identification and differentiation of 20 brands of lip balms. Moreover, lip balms on different substrates and wearing effects over time were also investigated. The results show that the PCA-LDA training accuracy was 92.5%, whereas the validation accuracy comes out to be 83.33%. A blind study using pristine samples was also performed which resulted in 80% PCA-LDA accuracy. PCA-LDA prediction of samples on various substrates showed a higher chemometric prediction accuracy for nonporous substrates (glass, plastic, and steel), than for porous substrates (cotton cloth, cotton swab stick, dry tissue paper, and white paper) for samples kept in room temperature and under sunlight for 15 days. The substrate study showed that the samples from various substrates could effectively generate respective spectra which can help in brand-level identification even after several days. The present method demonstrates a potential for lip balm samples to be used in forensic casework applications.     

Enhancement of Aged and Denatured Fingerprints Using the Cyanoacrylate Fuming Technique Following Dusting with Amino Acid‐Containing Powders

We have carried out experiments to investigate the aging of latent fingerprints deposited on black PVC over a period of 4–15 weeks. A thumbprint was used in each case and before deposition of the print the donor rubbed their thumb around their nose to add sebaceous deposits. We have studied the effect of heat, light, and moisture and we find that moisture is the most significant factor in the degradation of the latent print. We have attempted to enhance these latent prints by dusting with valine powder or powders composed of valine mixed with gold or red fluorescent commercial fingerprint powders. To make a direct comparison between “treated” and “untreated” prints, the prints were cut in half with one‐half being “treated” and one‐half not. Our studies show the best results being obtained when powders of valine and red fluorescent powders are applied prior to cyanoacrylate fuming.     

Repeatability and reproducibility of earprint acquisition

For all forensic disciplines dealing with identification -- e.g., of glass, tool marks, fibers, faces, fingers, handwriting, speakers -- in which manual (subjective, nonautomated) processes play a role, operator dependencies are relevant. With respect to earprint identification, in the period 2002-2005, the Forensic Ear Identification research project collected a database of 1229 donors, three prints per ear, and laid down a "best practice" for print acquisition. Repeatability and reproducibility aspects of the print acquisition are tested. The study suggests that different operators may acquire prints of differing quality, with equal error rates of the matching system ranging from 9% to 19%. Moreover, it turns out that "matching" earprints are more alike when taken in a consecutive row than when taken on separate occasions. This underlines the importance of (1) studying operator effects, (2) operator training, and (3) not gathering "matching" reference material at the same occasion.     

The Accuracy and Applicability of 3D Modeling and Printing Blunt Force Cranial Injuries

The purpose of this study was to determine the factors affecting the accuracy of 3D models and 3D prints of cranial blunt force trauma, to evaluate the applicability and limitations of modeling such injuries. Three types of cranial blunt force lesions were documented (hinge, depressed, and comminuted) using three forms of surface scanning (laser, structured light scanner, and photogrammetry) at two different quality settings (standard and high). 3D printed models of the lesions were produced using two different materials (a gypsum‐like composite powder called VisiJet^® PXL and an acrylic engineered composite plastic called VisiJet^® M3 in crystal colour). The results of these analyzes indicate the prints in this study exhibit some statistically significant differences from the actual bone lesions, but details of the lesions can be reproduced to within 2 mm accuracy.     

Integrated DNA and Fingerprint Analyses in the Identification of 60‐Year‐Old Mummified Human Remains Discovered in an Alaskan Glacier

Abstract: This report describes the identification of a merchant mariner who perished in 1948 when Northwest Airlines Flight 4422, a DC‐4 carrying 24 seamen and six crew members crashed into Mount Sanford, Alaska. Fifty‐one years later, a human forearm and hand were found close by the wreckage of the plane, prompting identification efforts using DNA and fingerprints. There were significant challenges to both the fingerprint and DNA analyses. The hand was badly desiccated, making fingerprint friction‐ridge detail almost invisible and the remains had been embalmed upon discovery, making DNA amplification difficult. We present the results of an interdisciplinary approach that successfully addressed these challenges and ultimately led to the identification of the remains. These efforts relied on efficient fingerprint rejuvenation and imaging techniques that improved print resolution, as well as new DNA extraction techniques optimized for aggressively embalmed remains.     

Chemical Differences Are Observed in Children’s Versus Adults’ Latent Fingerprints as a Function of Time*

Abstract: The identification of aged latent fingerprints is often difficult, especially for those of children. To understand this phenomenon, the chemical composition of children’s versus adults’ latent fingerprints was examined over time using Fourier transform infrared microscopy. Hierarchical cluster analysis revealed that children’s and adults’ prints were distinguishable for up to 4 weeks after deposition, based on differences in sebum composition. Specifically, adults had a higher lipid content than children, but both decreased over time, attributable to the volatility of free fatty acids. The aliphatic CH₃, aliphatic CH₂, and carbonyl ester compositions changed differently in adults versus children over time, consistent with higher cholesterol and cholesteryl esters in children’s prints and wax esters and glycerides in adults’ prints. Thus, fingerprint composition changes with time differently in children versus adults, making it a sensitive metric to estimate the age of an individual, especially when the age of the print is known.