Immunofixation of complement component C3 phenotypes in bloodstains after cellulose acetate electrophoresis

The determination of the polymorphic C3 phenotypes was accomplished by electrophoresis on cellulose acetate followed by immunofixation. The three common phenotypes resulting from the two common codominant alleles C3S and C3F were clearly distinguishable in blood and bloodstain samples. Storage and degradation of C3 in blood samples as well as the stability of C3 in dried bloodstains is discussed.     

Time-dependant changes in the protein spectrum of blood from living and dead persons by means of electrophoresis in cellulose acetate (author's transl)]

Protein electrophoresis on cellulose acetate shows that with increasing hemolysis of ante- and postmortem blood an extragradient in the alpha2-globulin field is created, which influences the relative ratio in the electropherogram. Strong hemolysis causes a fusion of the alpha2- and beta-globulin chains. In order to make a diagnostic interpretation of postmortem blood by means of electrophoresis, it is therefore essential to take into account the degree of hemolysis. Changes in the electrophoretic pattern due to hemolysis correlate best with the period of storage. With regard to timing for forensic purposes, the value is, however, limited.     

A microthermal resistance method for determining the species specificity of protein

Microthermal method was used for objective registration of the course of precipitation reaction in determination of species specificity of human and animal protein. This method allows one to carry out species identification using blood protein dilution with 0.9% solution of natrium chloride in correlation from 1:100 up to 1:100,000. Method is simple in use, it can be operated on the apparatus of home production. Analysis with two control tests may be performed within 10 min. The results of reaction are successfully registered as thermistograms subjoined to the expert conclusion.     

Rapid phenotyping of the group specific component by immunofixation on cellulose acetate

Determination of the genetically controlled variants of the polymorphic Gc system was achieved by electrophoresis on cellulose acetate membranes followed by immunofixation with a specific anti-Gc antiserum. The method is applicable to plasma, whole hemolyzed blood, and dried blood. Multiple specimens can be analyzed simultaneously within 60 to 80 min. The cellulose acetate electrophoretogram of the Gc variants remains as a permanent record.     

Phenotyping of carbonic anhydrase II in fresh blood and bloodstains on cellulose acetate membrane

A simple and rapid procedure is presented for the identification of carbonic anhydrase II in fresh blood and bloodstains using cellulose acetate membranes. Identification of the phenotypes is simplified by the migration of the isozyme bands to one side and away from the origin.     

Determination of group specific component phenotypes in dried bloodstains by immunofixation on cellulose acetate.

Group specific phenotypes in dried bloodstains can be rapidly and reliably determined by immunofixation on cellulose acetate membranes. Up to 16 samples, can be analyzed simultaneously in less than 60 min, and the cellulose acetate electrophoretogram is retained as a permanent record.     

20个STR基因座的种属特异性研究

确定本室常用的20个STR基因座对常见10种动物的种属特异性。20个STR基因座对10种常见动物血或软组织的DNA进行PCR扩增、聚丙烯酰胺凝胶电泳、银染色法进行DNA分型,测定各STR基因座的种属特异性。D11S554、SE33基因座对10种动物DNA,D12S391、CSF基因座对狒狒DNA,D19S253基因座对兔、狒狒的DNA,FGA基因座对兔、猴、狒狒的DNA均有PCR扩增产物,其片段长度与人的在同一电泳区内;DYS19基因座对10种动物的DNA,FES基因座对鸡DNA,PLA基因座对猪DNA,D21S11基因座对蛇、牛、狒狒的DNA有扩增产物,但其片段长度明显与人的不同。DYS389、DYS288、DYS390、D7S809、D13S631,TH01、vWA、AR、CD4、FABP基因座对鸡、猪等动物的DNA均无PCR扩增产物。在20个STR基因座中,10个基因座只对人DNA有扩增产物,有较好的种属特异性;8个基因座对人和部分动物均有扩增产物,但产物的片段大小与人的不同,在进行个人识别时,可以用其分析DNA的种属来源;2个基因座对人和动物均有扩增产物,且片段长度相近,在进行个人识别时,应先作DNA种属来源检查。