Lectin-histochemical detection of degenerative glycoconjugate deposits in human brain

Several lectins were used to study the localization of glycoconjugates in brain of elderly people and patients with Alzheimer type dementia (ATD) and Down's syndrome (DS). Five kinds of degenerated or deposited materials stained clearly by lectins specific to GalNAC, Gal, Fuc, and/or Man were recognized much in ATD and DS, less in elderly peoples, in addition to the binding of the lectins to neurons. (i) Round shape deposits called corpora amylacea (CA) which consisted of various sizes of round material, existed mainly on the surface of cerebral cortex and some in white matter of the brain. They were colored by Alcian blue (AB), Aldehyde fucsin (AF) and periodic acid shiff (PAS) and weakly by Hematoxylin (H), but not by Eosin (B). They showed clear reactivity with lectins specific to GalNAC, Gal, Fuc and Gal-GalNAC. (ii) Amorphous and variform amyloid deposits existed around blood vessels in the white matter were stained by thioflavin and lectins specific to GalNAC, Gal and Fuc, but not with Man specific lectins and PAS, AB, AF and HE. (iii) Another kind of amyloid deposits which showed a similar characteristic to the previous one and were recognized mainly in white matter and independent blood vessels. These deposits were stained by thioflavin but not by PAS, AB, AF and HE and showed good reactivity with lectins specific to GalNAC, Gal, Fuc, Gal-GalNAC, Gal-GIcNAc and Man. The reactivity with lectins specific to Gal, Fuc, and Man was seen in senile plaques (iv) and neurofibrillary tangles (v). Although at present we are unable to explain the origin of these deposits, it is clear from this study that the glycoconjugates form an integral part of the degeneration in the brain. The lectin staining with GS-I is useful in the forensic pathology to diagnose brain disorders at postmortem examination, since these lectin were able to detect five types of degeneration changes and/or deposits.     

An unusual variant of blood group A

A blood specimen from a forensic science case appeared to violate Landsteiner's Rule. The red cells failed to react with anti-A, anti-B, or O serum while reacting strongly with Ulex europaeus lectin but not other anti-A lectins. The saliva from the person involved was found to contain both A and H blood group substances in a ratio of 4:1. The blood group was determined to be type Am.     

Evaluation of antisera for bloodstain grouping. I. ABH, MN, and Rh

Sixty-eight different commercially available blood grouping antisera and lectins with ABH, MN, and Rh D, C, E, c, and e specificities were serologically evaluated for their applicability to bloodstain antigen determination. The characteristics of the antisera were determined with red cells, with fresh bloodstains, and with series of aging bloodstains. The Rh antisera were tested under a variety of serological conditions and with bloodstains on various substrata. Additionally, studies on optimization of absorption-elution procedure variables were carried out, and some data on the storage characteristics of red cells and blood grouping antisera were gathered.     

Study on expression of the glycoprotein in myocardial cell membrane of the rat's early myocardial ischemic

OBJECTIVE: To explore expression of the glycoprotein in early myocardial ischemic. METHODS: The glycoprotein changes occurred at the early acute cardiac ischemic area induced experimentally by ligation of left coronary artery of 32 SD rats. 6 lectins were measured by means of immunohistochemical methods. RESULTS: Positive staining of PNA could be observed in ischemic area at 5 min after ischemia, and the positive area increased with the prolongation of ischemic period. It became the strongest for 2 h and then decreased. CONCLUSION: This experiment proved that myocardial cell membrane in ischemia expressed D-galactose. This may be of some value in forensic medicine practice.     

Human-type blood group activities on chimpanzee erythrocytes with special reference to M and N

Human-type blood group activities on the red blood cells (RBCs) of three chimpanzees were individually examined with commercial mouse monoclonal antibodies (anti-A, -B, -H, -M, -N, -Lea, and -Leb) as well as lectins (UEA-I and VGA) and conventional polyclonal antisera for the systems ABO, MN, Lewis, Rh-Hr, P, Kell, Kidd, Duffy, and Lutheran. For further analysis of the MN antigens, treatment of the RBCs with sialidase, trypsin, and chymotrypsin were employed. The activities recognized among the three chimpanzees were A, H, M, N, Leb, c, S, k, and Jka. The RBCs of the three individuals possessed the A antigen which showed the same serologic activity as the human A1. Those chimpanzee RBCs showed higher H-activity than the human A1 RBCs. The Lewis b activity was revealed by the absorption-elution method. The RBCs of the three individuals showed a reactivity to the polyclonal anti-M reagents, which was affected by both the sialidase and trypsin treatment. The RBCs of two individuals were agglutinated with the monoclonal anti-N. The receptor was sensitive to sialidase and chymotrypsin. The RBCs of the three individuals, however, did not react with the monoclonal anti-M or with one of the polyclonal anti-N. These results indicate structural differences in the glycophorins and MN antigens between the human and chimpanzee.     

Über Methoden zur Darstellung von latenten Fingerabdrücken auf der menschlichen Haut

Five methods are described for the detection of latent fingerprints on human skin: the X-ray method, the application of laser radiation, the iodine-silver plate transfer method, development with iron powder and Dakty-foil, as well as the photographic paper lift technique. Experiences with the application of these methods in forensic investigations are reported, and impeding factors are outlined. The simpler methods (the detection of such prints with iron powder and Dakty-foil as well as the photographic paper lift technique) are to be preferred for the investigation at the scene of the crime.     

Genipin--a novel fingerprint reagent with colorimetric and fluorogenic activity

Genipin, the hydrolytic product of geniposide, which is extracted from gardenia fruit, shows good potential as a fingerprint reagent. It develops latent fingerprints on paper as blue impressions with good contrast and resolution. Even very faint impressions that are barely visible in ambient light will fluoresce brightly upon illumination at ca. 590 nm and are best viewed with a barrier filter above 630 nm. Potential advantages of genipin are the combination of colorimetric and fluorogenic activity in one reagent as well as its being a safe and environmentally friendly natural product.     

Governmental corruption in Africa: Sierra Leone as a case study

This paper examines the definition and context of official corruption in the emerging African nation of Sierra Leone. Historical, legal and sociological studies of the development of Sierra Leone and corruption within that nation are reviewed, as well as the content of official ljudicial inquiries into administrative corruption. These investigations and studies are supplemented with data from the primary author's experiences as a prosecutor and judge in Sierra Leone. Predisposing factors which facilitate corruption are identified and a variety of policies aimed at reform are discussed. This revised version was published online in August 2006 with corrections to the Cover Date.     

Massive acute arsenic poisonings

Arsenic poisonings are still important in the field of toxicology, though they are not as frequent as about 20-30 years ago. In this paper, the arsenic concentrations in ante- and post-mortem materials, and also forensic and anatomo-pathological aspects in three cases of massive acute poisoning with arsenic(III) oxide (two of them with unexplained criminalistic background, in which arsenic was taken for amphetamine and one suicide), are presented. Ante-mortem blood and urine arsenic concentrations ranged from 2.3 to 6.7 microg/ml, respectively. Post-mortem tissue total arsenic concentrations were also detected in large concentrations. In case 3, the contents of the duodenum contained as much as 30.1% arsenic(III) oxide. The high concentrations of arsenic detected in blood and tissues in all presented cases are particularly noteworthy in that they are very rarely detected at these concentrations in fatal arsenic poisonings.     

Post-traumatic meningitis: histomorphological findings, postmortem microbiology and forensic implications

Infections of the leptomeninges with the infectious agent gaining access to the intracranial compartment by traumatic means are termed post-traumatic. In cases with fatal outcome, the manner of death has to be classified as non-natural. Six cases of post-traumatic meningitis as the cause of death from the archives of the Institute of Legal Medicine in Hamburg, Germany with histological and microbiological investigations are presented. There were all males, age varying between 24 and 90 years (mean 58 years); range of the interval between original trauma and beginning of symptoms was 2 days up to 8 years; in 50% of the cases meningeal swabs yielded Streptococcus pneumoniae. Findings concerning origin and mechanism of post-traumatic meningitis as well as microbiological studies are compared with selected cases from the literature.     

疑难案件的法哲学争议——一种思想关系的视角

传统法理学将疑难案件当然地视为审判方法论的研究范畴,实际上这是一种误解,疑难案件不仅与审判方法论紧密相连,同时还不可避免地牵涉到法哲学的内容,此外它还是打通一般法理学与审判方法论的重要桥梁.     

Attitudes Toward Electronic Monitoring: An Exploratory Analysis Among Criminal Justice Students in Bosnia and Herzegovina

The purpose of this research study is to examine attitudes associated with the use of electronic monitoring as a criminal justice sanction in Bosnia and Herzegovina. Utilizing self-reported survey data from 57 graduate students enrolled in a criminal justice policy based course at the University of Sarajevo, students’ attitudes toward electronic monitoring are assessed. Specifically, students’ personal views about whether electronic monitoring meets the sentencing goals of deterrence, incapacitation, retribution, rehabilitation and reintegration are evaluated. Perceptions of the cost-effectiveness of electronic monitoring and the appropriateness of electronic monitoring as a sentence for specific offender types are also examined. Finally, the influence of student socio-demographic characteristics on opinions are also assessed. As a whole, students surveyed appear to support the use of electronic monitoring for juvenile offenders and offenders awaiting trial. Additionally, respondents do not view the conditions associated with electronic monitoring as all that negative or obtrusive. Implications from these findings, as well as limitations and suggestions for further research are discussed.     

IRS focuses on employment status of hospital-based physicians

In view of these serious consequences and the IRS' renewed interest in hospital-based physicians, it is imperative that all hospitals examine their contractual relationships with physicians under the foregoing standards to ascertain whether any physicians are improperly being characterized as independent contractors. Of particular concern are arrangements with aspects similar to those in TAM 9443002. Hospitals operating in states that still prohibit the employment of physicians are not necessarily protected, as the IRS does not accept the corporate practice of medicine doctrine as a defense to characterization of physicians as employees for tax purposes. In those states, it is probably best to handle problematic situations through the use of professional corporations, as discussed above.     

Increased lung weight in fatal intoxications is not unique to opioid drugs

Fatal intoxications with opioids are known to be associated with an increased lung weight, as well as with brain and pulmonary edema and urinary retention. However, there is evidence to suggest that fatal intoxications with non-opioid substances are also associated with increased lung weight; however, the latter aspect has not been comprehensively analyzed. To determine to what extent opioid and non-opioid substances are associated with increased lung and brain weight, we studied these organs in cases where the cause of death was attributed to intoxication with a single agent. Using data from cases autopsied at the National Board of Forensic Medicine (NBFM) in Sweden from 2009 through 2019 where the cause of death was attributed to a single substance, we created models of combined lung weight and brain weight. The models used age and sex as predictors as well as nested varying effects for the specific intoxicant and category of intoxicant. Suicidal hanging with negative toxicology cases served as controls. The population majority was male among both intoxications (68%) and controls (83%). The most common single substance group was opioids. All tested substances were associated with heavier lungs than controls, with the largest effect in the opioid group. Our findings show that several substances are associated with increased lung weight and that among intoxication deaths there is no difference in expected brain weight between substances. Hence, heavy lungs, without a reasonable explanation, should prompt a broad toxicological screening.     

Psychodiagnostic testing of sex offenders: a comparative study

Two groups of offenders, one charged with sex crimes as well as with crimes of larceny, and the other charged with sex crimes only, are compared with respect to their demographic characteristics and their intelligence and diagnostic classification as determined via psychological testing.     

科学标准在社会和法庭科学证据中的应用

在当今的司法程序中越来越多地应用科学技术来证明争议事实,但这种应用也存在一些争议和问题。有关证据和证明程序规则的存在．在本质上,并没有同科学证据的应用和采纳在任何方式上存在冲突。相反,如果存在非认知性的程序规则,其结果是这样的规则应该被撤销。除了物理、化学、工程学和遗传学等“解释性科学”得以广泛应用之外,心理学、社会学...     

构建公权与私权平衡下的中国物权法

《物权法》乃是保护权利人物权、规范财产秩序之法,然因物权涉及社会公共利益和第三人的合法权益,现代财产所有权社会化的结果导致《物权法》中蕴含了大量的属于公法范畴的强制性规范,使得物权相较于其他私权而言更容易受到以保护公共利益为核心的公权力的干涉和限制。《物权法》一方面要保护私人的合法权利不受公权力的过度干预,另一方面又要防止私权的过度滥用。《物权法》本身的实践需要构建一个公权与私权平衡的环境。     

智识互转：印证规范解析

我国刑事证明的印证模式突出表现了如下特点,系争内容由不同来源的证据提供同一性印证、证明程度排他性要求可能促成采用灵活性取证手段等。《办理死刑案件证据规定》之中有多处关于审查证据、采信证据和对全案事实认定的＂印证＂条款,这些条款体现了如下特点：需要印证的对象——主要针对证人证言、被害人陈述、被告人供述和辩解等容易因为出庭而出现反复的证据形式以及间接证据;印证的目的——主要是解决庭审中翻证、翻供以及作证资格缺陷和利害关系人的证言效力问题;印证的条件错综复杂,而印证的效力有一种法定证据制度的痕迹,同时又因为其语言模糊而可能存在滥用裁量权的嫌疑。针对印证规定的限度,第一,需要把以量取胜和依靠主观真诚和正当程序为基础的以质取胜相结合;第二,需要把靠证据说话与辩论、说理和质证等形式的意见之争相结合。     

Role of Culture and Context: Ethical Issues in Research with Asian Americans and Immigrants in Intimate Violence

Ethical principles are often presented as universal, immutable rules. However, when conducting research with ethnic minority groups, such as Asian Americans and immigrants, ethical issues need to be placed within a sociocultural context as ethical responses are filtered through the specific value orientations and belief systems of the ethnic group under study. Further, when the group is a marginalized population, power dynamics complicate ethical principles of autonomy. The complexities are further accentuated with sensitive topics such as intimate violence or domestic violence. Consequently, ethical questions about autonomy, informed consent, confidentiality, limits to confidentiality, and protecting participants’ safety and reducing distress need to be at the forefront when planning intimate violence research. An overview of sociocultural context of Western biomedical ethics is presented and specific ethical issues that emerge when conducting intimate violence are discussed.