Age estimation in children by measurement of carpals and epiphyses of radius and ulna and open apices in teeth: a pilot study

Estimation of skeletal age using radiographic images is widely used in assessing biological growth in clinical and auxological studies. The most frequently used areas for age estimation in children and adolescents are tooth and wrist/hand, both giving good results with only a low level of radiation. This pilot study of a sample of 150 Italian children and adolescents aged between 5 and 15 years focused on analyzing the possible applications of the proportion of carpal area (Ca) and teeth mineralization as a criterion of age estimation. The regression model, describing age as a linear function of gender (g), the ratio between carpal bones area and carpal area (Bo/Ca) and the measurement of open apices, yielded the following equation [Formula in text]. The model explained 93% of the total variance (R(2)=0.93), the median of the absolute values of residuals (observed age minus predicted age) was 0.465 years, with an inter-quartile range (IQR) equalling 0.529 years, and a standard error of estimate of 0.73 years.     

Excretion of Delta9-tetrahydrocannabinol in sweat

The aims of this study were: first, to determine the accuracy of the Cameriere method for assessing chronological age in children based on the relationship between age and measurement of open apices in teeth and, second, to compare the accuracy of this method with the widely used Demirjian et al. method and with the method proposed by Willems et al. Orthopantomographs taken from white Italian, Spain and Croatian children (401 girls, 355 boys) aged between 5 and 15 years were analysed following the Cameriere, Demirjian and Willems methods. The difference between chronological and dental age was calculated for each individual and each method (residual). The accuracy of each method was assessed using the mean of the absolute values of the residuals (mean prediction error). Results showed that the Cameriere method slightly underestimated the real age of children. The median of the residuals was 0.081 years (interquartile range, IQR=0.668 years) for girls and 0.036 years for boys (interquartile range, IQR=0.732 years). The Willems method showed an overestimation of the real age of boys, with a median residual error of -0.247 years and an underestimation of the real age of girls (median residual error=0.073 years). Lastly, the Demirjian method overestimated the real age of both boys and girls, with a median residual error of -0.750 years for girls and -0.611 years for boys. The Cameriere method yielded a mean prediction error of 0.407 years for girls and 0.380 years for boys. Although the accuracy of this method was better for boys than for girls, the difference between the two mean prediction errors was not statistically significant (p=0.19). The Demirjian method was found to overestimate age for both boys and girls but the mean prediction error for girls was significantly greater than that for boys (p=0.024), and was significantly less accurate than the Cameriere method (p<0.001). The Willems method was better than that of Demirjian (p=0.0032), but was significantly less accurate than that of Cameriere (p<0.001).     

中国北方未成年人群牙龄推断研究

目的 运用Cameriere法和Willems法推断中国北方5～14岁人群牙龄,并比较两种方法牙龄推断的准确性.方法 收集西安交通大学口腔医院年龄在5～14岁的中国北方未成年人群拍摄的全口曲面断层片2000例,其中男性1000例,女性1000例.分别采用Cameriere法和Willems法进行牙龄推断,利用SPSS ...     

Age estimation by pulp/tooth ratio in lower premolars by orthopantomography

Accurate age estimation has always been a problem for forensic scientists, and apposition of secondary dentine is often used as an indicator of age. Since 2004, in order to examine patterns of secondary dentine apposition, Cameriere et al. have been extensively studying the pulp/tooth area ratio of the canines by panoramic and peri-apical X-ray images. The main aim of this paper is to examine the relationship between age and age-related changes in the pulp/tooth area ratio in monoradicular teeth, with the exception of canines, by orthopantomography. A total of 606 orthopantomograms of Spanish white Caucasian patients (289 women and 317 men), aged between 18 and 75 years and coming from Bilbao and Granada (Spain), was analysed. Regression analysis of age of monoradicular teeth indicated that the lower premolars were the most closely correlated with age. An ANCOVA did not show significant differences between men and women. Multiple regression analysis, with age as dependent variable and pulp/tooth area ratio as predictor, yielded several formulae. R(2) ranged from 0.69 to 0.75 for a single lower premolar tooth and from 0.79 to 0.86 for multiple lower premolar teeth. Depending on the available number of premolar teeth, the mean of the absolute values of residual standard error, at 95% confidence interval, ranged between 4.34 and 6.02 years, showing that the pulp/tooth area ratio is a useful variable for assessing age with reasonable accuracy.     

Variations in pulp/tooth area ratio as an indicator of age: a preliminary study

This paper details a method for age determination of adults from single rooted teeth. The sample consisted of 100 Italian white Caucasian patients (46 men, 54 women) aged between 18 and 72 years. The single rooted maxillary right canine was utilized in this preliminary study. Pulp/root ration, tooth length, pulp/tooth length ratio, pulp/tooth area and pulp/root width ratios at three different levels were computed. Pearson's correlation coefficients between age and these variables showed that the ratio between pulp and tooth area correlated best with age (r2 = 0.85). Stepwise multiple regression models yielded a linear relationship between pulp/root width at mid-root level and chronological age and a linear relationship when pulp/tooth area was compared to age. Statistical analysis indicated that these two variables explain 84.9% of variations in estimated chronological age. The median of the absolute value of residual errors between actual and estimated ages was less than four years.     

Age estimation by measurements of developing teeth: accuracy of Cameriere's method on a Brazilian sample

Developing teeth are commonly the criteria used for age estimation in children and young adults. The method developed by Cameriere et al. (Int J Legal Med 2006;120:49-52) is based on measures of teeth with open apex, and application of a formula, to estimate chronological age of children. The present study evaluated a sample of panoramic radiographs from Brazilian children from 5 to 15 years of age, to evaluate the accuracy of the method proposed by Cameriere et al. The results has proven the system reliable for age estimation, with a median residual error of -0.014 years between chronological and estimated ages (p = 0.603). There was a slight tendency to overestimate the ages of 5-10 years and underestimate the ages of 11-15 years.     

基于数据挖掘实现成人喉软骨和舌骨CT图像重组的年龄推断

目的 运用数据挖掘技术探索喉软骨和舌骨CT图像重组用于成人年龄推断的可行性.方法 收集413例颈部薄层CT图像,样本年龄范围18～<80岁,将样本随机分为测试集和训练集.参照TURK等的分级方法,对所有样本进行全方位综合阅片分级,将甲状软骨骨化过程分为6级,环状软骨骨化过程分为5级,舌骨大角和舌骨体的骨性结合分为3级....     

数字X线摄影测量活体脊柱胸段推算身高

目的建立人脊柱胸段长度推算身高的方法。方法应用数字X线摄影技术（DR）摄制514例中国四川汉族人群的胸部DR片，测其脊柱胸段长度，同时准确测量被研究者身高，对测量获得的脊柱胸段长度与身高数据分组进行线性回归分析，建立胸段长度推算身高的回归方程。结果建立了混合组、男性组、女性组、20—45岁男性组、大于45岁男性组、20～45岁女性组和大于45岁女性组7个回归方程，经显著性检验，均有统计学意义（P〈0．01），复相关系数（R）范围0．834～0．624，回归系数（b）范围0．259～0．389，回归方程估计值的标准误在3．88～4．72cm范围内。结论利用人类脊柱胸段长度推算身高的方法在法医学个人识别中具有重要价值。     

西南地区汉族男性CT颅面径线与身高的关系

目的通过CT测量西南地区汉族男性颅面径线,建立该地区汉族男性颅面径线与身高的回归模型,为法医人类学研究积累数据。方法收集273例西南地区汉族男性头部CT资料,确定颅面部7条径线,运用图像后处理软件进行多平面重建和容积再现,对所选径线进行测量。对各测量指标与身高的相关性运用SPSS 21.0进行分析,建立推断身高的回归方程,并重新选取50例样本代入该数学模型进行检验。结果建立了7条径线的线性回归方程(P0.05);一元线性回归方程相关系数为0.190~0.439,标准估计误差(SEE)为4.597~5.023 cm;多元线性回归方程复相关系数为0.494~0.524,SEE为4.418~4.458 cm。回代检验得y=83.959+3.589 x_6+2.573 x_2,±1SEE准确率最高,为30%;y=72.646+3.316 x6+1.586 x_2+1.553 x_4+2.211 x_3,±2SEE准确率最高,为92%。结论本研究所选取的7条径线与身高均有明显的直线相关性,所建立的多元回归方程适用于推断西南地区汉族男性身高。     

Accuracy of four dental age estimation methodologies in Brazilian and Croatian children

ObjectiveTo compare and analyse the accuracy of four age estimation methods using the mineralisation stages of the permanent teeth (Cameriere et al. [16] [CAM], Liliequist and Lundberg [LLH] and Nolla without third molars [NOL7] or with them [NOL8]) in a mixed population of Brazilians and a homogeneous population of Croatians.MethodsOrthopantomograms of 930 Brazilians (366 males and 564 females) and 924 Croatians (365 males and 556 females) aged between 8 and 14.99 years were analysed using the CAM, LLH, NOL7 and NOL8 age estimation methodologies.ResultsLLH presented the best absolute differences (ADs) among both populations without sex stratification, while CAM presented the worst results. In addition, the mean differences revealed underestimations, except when the LLH and NOL7 methods were used for the Brazilians. When the sample was stratified by sex, the best AD values were found with NOL7 (0.80) for the Brazilians and with LLH (0.98) for the Croatians. When the sample was stratified by sex and age, CAM presented high accuracy at the early ages, and LLH presented high accuracy at the older ages. The results obtained with the Nolla methods (NOL7 and NOL8) were mostly similar, but NOL7 yielded slightly better results.ConclusionsThe values for the Brazilians and the Croatians were relatively similar, and the techniques were properly applied in both population samples. The best method for evaluating both countries was LLH, followed by NOL7, NOL8 and CAM.     

Age estimation by pulp/tooth ratio in canines by mesial and vestibular peri-apical X-rays

Changes in the size of the pulp canal, caused by apposition of secondary dentine, are the best morphometric parameters for estimating age by X-rays. The apposition of secondary dentine is the most frequently used method for age estimation in adult subjects. In two previous papers, we studied the application of the pulp/tooth area ratio by peri-apical X-rays as an indicator of age at death. The aim of the present study was to test the accuracy of age evaluation by combined analysis of labio-lingual and mesial peri-apical X-rays of lower and upper canines. A total of 200 such X-rays were assembled from 57 male and 43 female skeletons of Caucasian origin, aged between 20 and 79 years. For each skeleton, dental maturity was evaluated by measuring the pulp/tooth area ratio according to labio-lingual and mesial X-rays on upper (x(1), x(2)) and lower (x(3), x(4)) canines. Very good agreement was found between intra-observer measurements. Statistical analysis showed that all variables x(1), x(2), x(3), and x(4) and the first-order interaction between x(1) and x(3) contributed significantly to the fit, so that they were included in the regression model, yielding the following regression formula: Age = 120.737 - 337.112x(1) - 79.709x(2) - 364.534x(3) - 65.655x(4) + 1531.918x(1)x(3) . The residual standard error of estimated ages was 3.62 years, with 94 degrees of freedom, and the median of the residuals was -0.155 years, with an interquartile range of 4.96 years. The accuracy of the method was ME = 2.8 years, where ME is the mean prediction error. The model also explained 94% of total variance (R(2) = 0.94).     

放射影像学方法测量活体女性颈椎推算身高

目的测量颈椎高度建立四川汉族女性人群的身高推算方程，为法医学个人识别提供帮助。方法应用计算机X线摄影放射学方法，在209例四川汉族女性颈椎CR片上测量C3～C7椎体高度，并准确测量身高。按年龄是否大于45岁进行分组，对各颈椎的测量数据与身高进行线性回归分析，建立颈椎推算身高的回归方程。结果共建立一元回归方程36个，多元回归方程12个，所有回归方程经线性回归模型假设检验，均有统计学意义（P〈0．05）。所建立的回归方程的复相关系数（r）在0．628～0．280范围内，回归方程的标准误在4．03～5．03cm范围内。结论本研究所建立的回归方程，可用于四川汉族女性颈椎的身高推算。     

Age-at-death estimation by pulp/tooth area ratio in canines: study of a 20th-century Mexican sample of prisoners to test Cameriere's method

Accurate age estimation has always been a problem for forensic scientists, and apposition of secondary dentine is often used as an indicator of age. Cameriere et al. studied the pulp/tooth area ratio by peri-apical X-ray images of the canines, to observe the apposition of secondary dentine. The present study examines the application of this technique in a Mexican identified sample coming from the Department of Physical Anthropology of the INAH, at Mexico City. The main aim of this work is to test the reliability of this method in a skeletal sample of a specific population, different from the samples used for its development. The obtained regression model explained 96.2% of total variance (R(2) = 0.962) with a standard error of estimate of 1.909 and a standard deviation of 1.947. These results demonstrate great reliability and that the age/secondary dentine relationship is not variable in this specific population.     

Estimation of age at death by tibial osteon remodeling in an autopsy series

Estimation of age at death by histological methods in tibiae has been found to be inaccurate in individuals less than 55 years of age. This study describes reasons for the inaccurate age estimates and provides a new regression equation for age estimation. Bone cores were removed from tibiae in 53 individuals at autopsy ranging in age from 17 years to 53 years of age; 48 males and 5 females. The sample contained 41 whites and 12 blacks. Variables ascertained from each core were: cortical thickness, cortical bone density, secondary osteon number, area, and perimeter, and Haversian canal area and perimeter. The results showed significant differences in secondary osteon number, area, and perimeter between groups aged less than and greater than 35 years of age. Multiple linear regression analysis indicated osteon number to be the best predictor of age in this sample aged less than 55 years of age. Age at death was accurately estimated for 11 additional forensic cases using osteon number in tibiae. No significant histological differences were observed between males and females and between blacks and caucasians.     

Fourier transform infrared reflectance spectra of latent fingerprints: a biometric gauge for the age of an individual

To determine if changes in fingerprint infrared spectra linear with age can be found, partial least squares (PLS1) regression of 155 fingerprint infrared spectra against the person's age was constructed. The regression produced a linear model of age as a function of spectrum with a root mean square error of calibration of less than 4 years, showing an inflection at about 25 years of age. The spectral ranges emphasized by the regression do not correspond to the highest concentration constituents of the fingerprints. Separate linear regression models for old and young people can be constructed with even more statistical rigor. The success of the regression demonstrates that a combination of constituents can be found that changes linearly with age, with a significant shift around puberty.     

Age estimation from quantitation of features of "chest plate" X-rays

This study presents a method for estimating the age at death from the quantitation of roentgenologic features of X-ray films of chest plates obtained during routine autopsies. Multiple linear regression analysis allows estimation of coefficients of regression of features on known age-at-death individuals. The regression equation can be used in turn for age estimation of an unknown age-at-death individual. The accuracy of age estimation is about +/- 8.4 years (standard error) which is in the range of previously published macroscopic methods, though the present method is much faster and simpler.     

Accuracy of BR Regression Equation for Impacted Teeth in Age Estimation in Haryana Population of India

Abstract:  Dental cementum is a vital tissue that demonstrates continuous apposition throughout the life of a tooth. Positive correlation has been reported between the coronal displacement of cementum and age in impacted teeth and BR regression equation has been proposed. The present study is an attempt to calculate accuracy of BR regression equation in Haryana population of India. The study was conducted on 20 impacted mandibular third molars extracted from healthy patients, aged between 22 and 32 years. The bucco-lingual ground sections were prepared and the distance between the edges of enamel and cementum were measured with micrometer attached to a light microscope and BR regression equation was applied for age estimation. Although the estimated age with BR regression equation was less than chronological age it was not statistically significant. Hence, the BR regression equation can be used in medicolegal as well as in forensic odontology for age estimation.     

Radiographic evaluation of third molar development in relation to chronological age among Turkish children and youth

A sharp increase in forensic age estimation of living persons has been observed in recent years. However, ethnic populations residing in different countries have been insufficiently analyzed. According to 2004 data compiled by the Essen-based Turkey Research Center, there are 3.8 million Turkish people living abroad, and 3.2 million of them reside in European Union countries. Despite the high number of Turks living abroad, little is known about third-molar development for forensic application in this population. Hence, it was considered worthwhile to determine the developmental stages of the third molar in a group of Turkish population, to assess chronological age estimation based on the developmental stages, and to compare third molar development according to sex, age and location. Orthopantomograms of 1134 Turkish patients, ages 4-20 years were examined and third-molar developmental stages were evaluated based on Demirjian's classifications. Orthopantomograms were scored by two different observers, and Wilcoxon matched-pairs signed-ranks test used to test intra- and inter-observer reliability revealed a strong agreement between both intra- and inter-observer measurements. Linear regression analysis was performed to correlate third-molar development and chronological age, and further statistical analysis was performed to determine the relation between sex, age and location. Results showed a strong linear correlation between age and molar development (males: r(2)=0.57; females: r(2)=0.56). Mineralizations of left and right third molars were compared using Wilcoxon tests, and no statistical differences were found. No significant differences were found in third-molar development between males and females. Mandibular third molar crypt formation was observed in 2.4% of patients at age seven and maxillary third molar crypt formation was observed in 1.3% of patients at age seven. A strong correlation was found between third-molar development and chronological age. Among the Turkish population, third molar crypt formation is observable at as early as 7 years in both the mandible and maxilla. Agenesis can be determined conclusively if no radiolucent bud is present by age 14.     

Alcohol ingestion and age of death in hanging suicides

Retrospective review of 100 consecutive, nondecomposed cases of suicide because of hanging was conducted at Forensic Science SA, Australia. Alcohol was detected in 38 cases (38%). The percentage of victims who had ingested alcohol significantly decreased with age (r(2)=0.81), with alcohol detected in 57.1% of those aged ≤24 years, compared to 28.5% of those aged between 55 and 64 years, and 0% of those aged ≥65 years. A similar linear relationship between alcohol ingestion and age was found for cases with blood alcohol levels >0.05 g/100 mL (r(2)=0.73). The mean ages of those with detectable alcohol (35.2 years) levels >0.05 g/100 mL (35.1 years) and levels >0.1 g/100 mL (37.2 years) were all significantly less than in those with no detectable alcohol (44.4 years) (p<0.005, <0.005, <0.05 respectively). A clear relationship between alcohol ingestion and younger age was shown in hanging suicides.     

青少年肩关节X线片的骨龄研究

目的探讨青少年肩关节X线影像与年龄间的关系。方法对太原地区267名年龄在12～22岁的青少年拍摄肩关节X线片,根据X线片上的不同年龄特征,筛选出5个与年龄变化密切相关的影像标志点,进行分级评分,提出了肩关节X线片判定年龄的观察指标及分级标准,并使用SPSS包进行数据处理,建立了利用肩关节X线片判定青少年年龄的判别方程。结果判别方程y=9.517+0.518x1+0.913x2+0.465x3+0.436x4经方差分析,F>0.01,回归高度显著。将全部样本进行计算机方程回代检验,符合率为±1S72.01%,±2S96.3%,其结果达到了设计与统计学要求。方程具有实用价值。结论本项研究证实了青少年肩关节X线影像与年龄之间存在相关关系,并为司法鉴定时根据肩关节X线片判断年龄提供依据,同时也为人类学研究积累了资料。