共查询到17条相似文献,搜索用时 156 毫秒
1.
本文介绍先采用人唾液对粗制抗N血清做基础吸收后,再通过红细胞抗原进行精制的一种新方法。精制的抗N血清具有效价高、特异性好的特点,且减少了因用人红细胞重复吸收而引起的溶血反应。 相似文献
2.
3.
4.
5.
6.
笔者受理了一起李控告魏将其强奸案,魏拒不承认。李将擦拭阴部的一块旧衣片送检。旧衣片上检有精子,每视野可见2~3个不完整的精子,偶见少量的扁平上皮细胞。用凝集反应确定李血型为B型;李的丈夫张的血型为O型;魏的血型为B型。用中和试验确定李的丈夫唾液为非分泌型;李与魏的唾液均为B分泌型。取该衣片精斑处与无精斑处检材浸泡,用16倍效价的抗A、抗B、抗H血清做吸收抑制凝集检验,空白检材与精斑检材均出现抗B血清抑制。由于李、魏、张三人的血型物质均可混于其中,而检材空白又出现B型,考虑旧衣片有李的B型物质干扰。李的B型… 相似文献
7.
应用时间决定性荧光免疫测定法(TR-FIA法),对129例健康成人唾液中Lweis及H1血型物质进行定量检测。Le阳性个体均不同程度地检出了Lea和Leb物质。Lea物质含量:Le(a+b-)型>Le(a-b+)型;Leb物质含量:Le(a-b+)型>Le(a+b-)型。Le(a+b-)型的Lea物质>Leb,Le(a-b+)型的Leb物质>Lea物质。Le阴性的部分个体未能检出Lewis物质,其余个体也仅检出微量。根据Lea和Leb物质在唾液中的相对含量,可以推测红细胞Lewis型,并提示Leb物质可能由Lea物质转化而形成。 相似文献
8.
9.
作者以精浆特异蛋白P30为抗原免疫新西兰白兔、豚鼠和鸡三种实验动物,制备了抗P30血清。用双向琼脂扩散法检测兔和豚鼠的抗 P30血清,其特异性和敏感性均达到目前国外同类产品的水平。抗 P30血清与阴道分泌物,血清、唾液、尿液、初乳以及羊精液、鸡精液均不出现交叉反应。用抗 P30血清检测混合的人精浆,其抗原效价为1:160;P30含量可测到12.5ug/ml。在三种动物的抗血清中,豚鼠抗 P30血清的抗体效价最高。以不同浓度的 P30测豚鼠、兔和鸡抗 P30血清抗体效价豚鼠平均滴度可达52.50,兔次之,鸡的抗 P30血清最差。经作者制备的抗 P30血清可用来确证精液。 相似文献
10.
本文通过吸收抑制试验证明了抗人血红蛋白沉淀素血清中存在的那种能凝集人类红细胞的凝集素,实际上就是用人的红细胞免疫家兔而产生的抗人红细胞凝集素.为考察抗人红细胞凝集素血清对血痕种属的鉴识能力,本文用此抗血清作解离试验,对不同稀释度的新鲜人血痕、室内存放的久暂不一的人血痕和15种动物血痕进行了检测.得到的结果是,新鲜人血痕稀释至1/1000仍呈阳性;500例室内存放的人血痕中,存放时间在11年以下的448例全部能够准确测出;存放12-16年的52例人血痕中,有33例能够测出,余19例呈阴性;15种动物血痕全部呈阴性. 相似文献
11.
E I Deriugina Iu I Savel'ev A E Nosyrev M I Lapenkov T L Nikolaeva 《Sudebno-meditsinskaia ekspertiza》1992,35(2):23-26
The authors suggest a simple sensitive technique for enzyme immunoassay (EIA) of ABH antigens in saliva and semen. A two-staged dot blot solid-phase EIA on nitrocellulose membranes was employed with anti-ABH monoclonal antibodies obtained in immunization of mice with human red cells. 4-chloro-1-naphthol substrate solution was used to visualize the peroxidase label. The results of analysis of salivary and spermatic samples obtained from donors of various groups evidence that this EIA variant may be useful in forensic medicine. 相似文献
12.
J L Mudd 《Journal of forensic sciences》1989,34(5):1082-1089
Using an enzyme-linked immunosorbent assay (ELISA), this study investigated the use of monoclonal antibodies for detecting secreted ABH blood group substances in semen and saliva. The results demonstrated that the behavior of some monoclonals were unpredictable and often failed to detect the corresponding antigen in a number of the specimens tested. The suitability of the monoclonal reagents for detecting soluble blood group antigens could not be predicted by their behavior with red cell antigens. Consequently, care must be taken in the selection of monoclonal reagents for use in the detection of secreted blood group antigens. 相似文献
13.
T Takatori Y Tsutsubuchi K Terazawa M Nagao H Akabame H Mikami 《Forensic science international》1990,47(3):261-268
The Lewis blood grouping of human saliva stains could be detected by an enzyme-linked immunosorbent assay (ELISA) using anti-Le(a) and anti-Le(b) monoclonal antibodies with an avidin-biotin complex (ABC). The saliva stains (1.0 by 1.0 cm in size) were used as samples and not only could the Lewis substances of 57 individual stains be correctly typed by this method, but also it was clarified that there are several different secretion patterns of amounts of Le(a) and Le(b) substances in 3 individual Lewis types. 相似文献
14.
15.
Kolokolova GP 《Sudebno-meditsinskaia ekspertiza》2006,49(4):32-34
Experimental spots of the saliva, sperm, vaginal secretion from persons with groups Ase, Bse, ABSe and Abse were studied with mixed agglutination reaction (MAR) using hemagglutinating sera anti-A and anti-B (heteroimmune, isoimmune), monoclonal antibodies. MAR with monoclonal antibodies was able to diagnose ABSe group not only in the spots of saliva, but also in the spots of sperm and vaginal secretion where heteroimmune sera failed. 相似文献
16.
M Ogata I Nakasono M Iwasaki S Kubo T Fukae H Suyama K Narita 《Journal of forensic sciences》1987,32(6):1551-1557
It is known that rabbit anti-gum arabic (GA) serum has cross-reactivity with Lea antigen, and that, by using this cross-reactive anti-Lea antibody, the presence of Lea antigen in red blood cells and saliva can be demonstrated with accuracy. We have devised a rapid and highly sensitive method for detecting Lea substance in human saliva by the enzyme-linked immunosorbent assay (ELISA) method using an anti-Lea antibody isolated from anti-GA serum by affinity chromatography on Synsorb Lea. The ELISA plate, coated with the specific anti-Lea antibody, adsorbed the Lea substance in saliva which was subsequently identified by adding enzyme labeled anti-Lea IgG in that order. The method could detect the Lea substance in Le(a+) saliva stains as small as 0.1 by 0.1 cm in size that had been stored at room temperature for three weeks and in Le(a+) saliva stains 0.7 by 0.7 cm in size that had been stored for ten years. This method seems to be useful for quantitative analyses of the Lea substance in various body fluids. 相似文献
17.
阐明非分泌型的基因型与血型物质分泌量和Lewis表型的关系。应用时间决定性荧光免疫测定法(TR-FIA),检测传统的A型Lewis阳性非分泌型个体唾液中H、A及Lewis抗原含量,并以序列特异性PCR,确定其基因型。非分泌型个体唾液中检出了高含量的Lea抗原,其中8例不同程度的检出了少量H、A和Leb抗原,其FUTZ位点为se2/se2纯合型,属Le(a+b+)型;1例基因型为se3/se5杂合型,未能检出H、A及Leb抗原,属Le(a+b-)型。se2是弱分泌基因,se3及se5是非分泌基因。 相似文献