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1.
Wei Cui Xiaoye Jin Yating Fang Qiong Lan Jiangwei Lan Man Chen Shuyan Mei Tong Xie Bofeng Zhu 《法庭科学研究（英文）》2022,7(4):694
In this research, genotyping data of 43 InDel loci in 311 Han individuals in Ankang City, Shaanxi Province, China were detected using a self-developed five-dye multiplex amplification panel. The allelic frequencies and forensic parameters of all InDel loci were calculated. The combined power of discrimination and probability of exclusion values were 0.999 999 999 999 999 998 827 39 and 0.999 887 424, respectively, which demonstrated that this 43-InDel panel was powerful for individual identifications in Ankang Han population. Moreover, genetic distances, pairwise FST values, principal component analyses, phylogenetic trees and STRUCTURE analyses were performed to investigate the genetic affinities between Ankang Han and reference groups. Population genetic investigations indicated that Ankang Han population had a close genetic relationship with Southern Han population compared with other reference groups. 相似文献
2.
目的通过对常染色体STR和X染色体STR基因座进行分型检验,探讨姑侄、叔侄关系的鉴定策略。方法提取案例中被检女孩和另外3名个体（女性2名,疑为被检女孩的姑姑;男性1名,疑为被检女孩的叔父）的血样DNA,采用Goldeneye 20A系统和AGCU 21＋1系统分别进行常染色体STR基因座的复合PCR扩增,用Mentype○RArgus X-12试剂盒和本室自主研制的16重X染色体STR扩增体系分别进行X染色体STR基因座的复合PCR扩增,用3130 XL遗传分析仪进行毛细管电泳和基因型分析。结果依据常染色体STR基因型结果及姑侄、叔侄关系指数计算结果,不排除2名被检姑姑和与被检女性存在姑侄关系;不排除被检叔叔和与被检女性存在叔侄关系,X染色体STR分型结果支持此鉴定意见。结论对于姑侄、叔侄关系鉴定案例,X染色体STR基因座是常染色体STR基因座的良好补充,两者联合运用可获得可靠的鉴定意见。 相似文献
3.
《Forensic Science International: Genetics Supplement Series》2019,7(1):38-40
The She ethnic is a large minority in China with approximately 700,000 individuals. For more than one thousand years, they mainly resided in Zhejiang and Fujian provinces. To obtain a better understanding of the genetic background of She, we investigate 21 autosomal STRs (A-STRs) and 16 X-STRs in 296 unrelated healthy individuals from Zhejiang She population. Allele frequencies and forensic parameters prove these markers are useful for forensic application. We also investigate the genetic background with the two types of markers. Nei genetic distances between She and Eastern Han population are always the lowest, regardless of the markers used for analysis. Although the tested STRs are located on different chromosomes with different inheritance laws, A-STRs and X-STRs provided in general congruent phylogenetic signal and similar cluster among compared groups. These results demonstrated that geographic isolation and interactions play significant roles in differentiation of genetic constitution of ethnic groups. 相似文献
4.
Vincenzo Agostini 《法庭科学研究（英文）》2020,5(4):336
During World War II, many nations took part in the war. Among the supporters of the Alliance there was also Brazil. In August 1944, under the leadership of President Getúlio Vargas, Brazil declared war on Nazi Germany and took part in the Italian campaign by sending many troops to support the Allies in the Central Italy. Once the conflict was over, the deceased Brazilian soldiers were buried in Pistoia, a few kilometers from Florence. But only in 1960 the Brazilian government authorized the transfer of the dead soldiers to their homeland. Five years later, during the building of the Brazilian Military Votive Monument, still in the Pistoia cemetery, a last body was found but could not be identified: so he was buried as an “unknown soldier”. In December 2012, the Brazilian Embassy in Italy asked for performing forensic genetics analysis for identification purposes on the remains of this last unknown Brazilian soldier. After almost 70 years a complete short tandem repeats (STR) profile was obtained, useful for any relatives searching.Key points:
- Identification of the last Brazilian Unknown Soldier buried in Italy.
- DNA analysis on 70 years old skeletal remains.
- Brazilian soldier’s history during World War II.
5.
目的调查16个X-STR基因座在河南汉族人群中的遗传学数据,评估其法医学应用价值。方法应用Goldeneye~(TM)DNA身份鉴定系统17X试剂盒,对河南地区326名汉族无关个体DNA进行PCR扩增,3130xl型遗传分析仪电泳分析,Gene Mapper~ID-X软件分析等位基因片段大小。统计分析16个X-STR基因座的频率数据和群体遗传学参数,并与其他地区已有人群数据进行比较。结果在所检测的16个X-STR基因座中,DXS6800具有中度多态性,其余15个基因座均具有高度多态性。这16个X-STR基因座在女性群体的累积个人识别率为0.999 999 999 999 992,在男性群体的累积个人识别率为0.999 999 996 577 712,在三联体中的累积非父排除率为0.999 999 971,在二联体中的累积非父排除率为0.999 992 574。结论这16个X-STR基因座达到了法医物证学应用要求,尤其对特殊的亲权鉴定案件具有重要的应用价值。 相似文献
6.
Yijie Wang Xiaoye Jin Wenqing Zhang Wei Cui Tingting Kong Chong Chen Yuxin Guo Haotian Meng Bofeng Zhu 《法庭科学研究（英文）》2022,7(2):163
Short tandem repeats (STRs) play an essential role in forensic genetics due to their high degree of polymorphisms, wide distributions and easy detection method. In this study, allelic frequencies and forensic statistical parameters of the 19 autosomal STR loci in a Kazak ethnic group were calculated, and its genetic relationships with reference populations were assessed in order to understand population structure better and enrich population genetic data for forensic practice in Chinese Kazak ethnic group. There were 226 identified alleles with the corresponding allelic frequencies ranging from 0.0008 to 0.5295 in the 628 unrelated healthy Kazak individuals in Xinjiang Uygur Autonomous Region. All autosomal STRs were conformed to the Hardy-Weinberg equilibrium after Bonferroni’s correction. The cumulative power of discrimination and the combined probability of exclusion of all the 19 autosomal STRs were 0.999 999 999 999 999 999 999 997 162 and 0.999 999 994 484, respectively. Furthermore, the DA distances and Fixation index values of pairwise populations, principal component analysis, multidimensional scaling analysis, phylogenetic tree analysis and structure analysis were conducted to probe the genetic relationships between the Kazak group and other reference populations. The population genetic results showed that these 19 autosomal STR loci were characterised by high genetic diversities in the Kazak group. Furthermore, the studied Kazak group had close genetic relationships with the Uyghur group and the Uzbek group. The present results may facilitate understanding the genetic background of the Chinese Xinjiang Kazak group. 相似文献
7.
Short tandem repeats on the Y chromosome (Y-STRs), characterized by paternal inheritance, are valuable in forensic practice. Notably, the potential application of Y-STRs in pedigrees should be drawn upon, especially in China’s surname-concentrated natural villages. The study focused on 50 Y-STRs, including 13 rapidly mutating (RM) Y-STRs that largely constitute the current Y-STR commercial kits, and determined the differences in these Y-STRs between branches in a large pedigree and the discriminatory power of these haplotypes in different units for male relatives. As indicated in the results, 14 inconsistencies were observed at 9 Y-STRs between 10 father-son pairs. In addition, these 50 Y-STR haplotypes discriminated 10 out of 47 father-son pairs, 106 of 148 cousin pairs, 70 of 119 uncle-nephew pairs, 17 of 39 brother pairs, and 14 out of 33 grandfather-grandson pairs in a large pedigree. The RM Y-STR set is able to differentiate close male relatives in a large pedigree. 相似文献
8.
This article describes a newly devised autosomal short tandem repeat (STR) multiplex polymerase chain reaction (PCR) system for 19 autosomal loci (D12S391, D13S317, D16S539, D18S51, D19S433, D2S1338, D21S11, D3S1358, D5S818, D6S1043, D7S820, D8S1179, CSF1PO, FGA, TH01, TPOX, vWA, Penta D and Penta E), 27 Y-chromosome STR loci (DYS19, DYS385, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS449, DYS456, DYS458, DYS460, DYS481, DYS518, DYS533, DYS570, DYS576, DYS635, DYS627, YGATAH4 and DYF387S1) and amelogenin with six-colour fluorescent labelling. Various parameters were evaluated, such as its accuracy, sensitivity, specificity, stability, ability to analysis of mixtures and effects of changes in the PCR-based procedures. All of the 47 selected STR loci were accurately and robustly amplified from 282 bloodstain samples. The species-specificity was high and some ability to inhibit Hematin was identified. The lowest detectable DNA amount was ≥0.125 ng. All of the male loci of the secondary component were revealed precisely when the control DNA was mixed at male/female and male/male ratios of 1:4 or more. We conclude that the present 19-plex autosomal STR and 27 Y-STR assay is both accurate and sensitive. It constitutes an additional powerful tool for forensic applications. 相似文献
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11.
Bones and teeth often represent the only sources of DNA available for identifying human remains. DNA in bones and teeth is generally better preserved than that in soft tissues because of the presence of hard connective tissue with a high level of calcium. Because of the extensive mineralisation, the choice of an efficient DNA extraction procedure is important to minimise the sampling of a high level of minerals and to remove polymerase chain reaction (PCR) inhibitors. Some protocols are available for DNA extraction from bones and teeth as part of the Qiagen EZ1 DNA Investigator Kit using the EZ1 Advanced XL automated purification platform. To improve the efficiency of DNA extraction from skeletal remains, the present study focuses on a modification to these already available protocols. In this study, different bones and teeth collected between 1 and 50 years after death were subjected to DNA extraction using the standard EZ1 protocol, a supplementary protocol, and a modified protocol. The modified approach included a decalcification step, whereas the Qiagen protocols worked directly on non-decalcified powder. In all three procedures, 150 mg samples were used for DNA extraction. We evaluated the quantity of DNA recovered from samples, the presence of any PCR inhibitors co-extracted, the level of DNA degradation, the quality of short tandem repeat (STR) profiles, and the reproducibility of the modified procedure. When compared with the other protocols, the modified protocol resulted in the best recovery of DNA that was free of PCR inhibitors. Additionally, the STR profiles were reliable and of high quality. In our opinion, the decalcification step increases DNA recovery by softening tissues, which allows lysis solutions to act more effectively. Furthermore, the use of two lysis solutions and the variation added to the EZ1 purification step allow for DNA recovery with quality and quantity superior to those of the previously available Qiagen-based protocols. These findings may be helpful solutions to the problems commonly encountered when dealing with difficult samples, such as bones and teeth.
Key points
- Bones and teeth often represent the only sources of DNA for identifying human remains.
- The choice of an efficient DNA extraction procedure is important for maximizing DNA recovery and removing PCR inhibitors.
- This study focuses on modifications to the previously available Qiagen-based protocols.
- The modified protocol enabled the best recovery of DNA, and both quality and quantity were superior to those of the previously available Qiagen-based protocols.
- The STR profiles obtained from samples extracted using the modified protocol were reliable and of high quality.
12.
《法庭科学研究（英文）》2020,(1):中插4,32-37
Forensic anthropology casework frequently encounters evidence of animal scavenging asso-ciated with fragmentation and loss of skeletal material.Published resear... 相似文献
13.
Sreetharan Kanthaswamy Torsten Brendel Luis Cancela Denise A. Andrade de Oliveira Bertram Brenig Carmen Cons Julian A. Crespi Markta Dajbychov Andreas Feldl Tomohito Itoh Vincenzo Landi Amparo Martinez Malgorzata Natonek-Wisniewska Robert F. Oldt Anna Radko Oscar Ramírez Clementina Rodellar Manuel Ruiz-Girn David Schikorski María Elena Turba Guillermo Giovambatista 《法庭科学研究（英文）》2022,7(4):708
The probative value of animal forensic genetic evidence relies on laboratory accuracy and reliability. Inter-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their output. The International Society for Animal Genetics (ISAG) administered animal forensic comparison tests (AFCTs) in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification, parentage and species determination services. The AFCTs revealed that analyses of low DNA template concentrations (≤300 pg/µL) constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage results. Moreover, a lack of familiarity with species testing protocols, interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination results. Several laboratories showed improvement in their genotyping accuracy over time. However, the use of forensically validated standards, such as a standard forensic short tandem repeat (STR) kit, preferably with an allelic ladder, and stricter guidelines for STR typing, may have prevented some common issues from occurring, such as genotyping inaccuracies, missing data, elevated stutter products and loading errors. The AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each other. Laboratories should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number (LCN) analysis and species testing. Although this is the first time that the ISAG has conducted comparison tests for forensic testing, findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing. 相似文献
14.
Anthropological analysis of fragmentary evidence can be challenging but diverse methods allow substantial information to be gleaned. Scanning electron microscopy/energy dispersive X-ray spectroscopy enables determination if bone and/or tooth tissue is present. Protein radioimmunoassay or DNA analysis can establish the species present. Histological analysis can assist in species determination and reveal information about thermal changes. Radiocarbon analysis with special reference to the modern bomb-curve can clarify the postmortem interval. Anthropologists should also be aware that DNA analysis not only can enable positive identification but assist in the evaluation of sex and age at death. 相似文献
15.
Jing Liu Ziwei Ye Zheng Wang Xing Zou Guanglin He Mengge Wang Shouyu Wang Yiping Hou 《法庭科学研究（英文）》2022,7(2):189
With the characteristics of low mutation rate, length variation and short amplicon size, insertion/deletion polymorphisms (InDels) have the advantages of both short tandem repeats (STRs) and single nucleotide polymorphisms (SNPs). Herein, people of two ethnicities from Hainan Island were genotyped for the first time using the Investigator DIPplex kit. We investigated the forensic parameters of the 30 InDels and the phylogenetic relationships among different populations. The accumulated powers of discrimination and powers of exclusion were 0.999 999 999 9646 and 0.9897 in the Hainan Han population and 0.999 999 999 9292 and 0.9861 in the Hainan Li population, respectively. Additionally, population comparisons among geographically, ethnically and linguistically diverse populations via cluster heatmap, multidimensional scaling, principal component analysis, phylogenetic tree and STRUCTURE analyses demonstrated that the Hainan Han population had genetic similarities to the other Han, She and Tujia populations, while the Hainan Li population had close genetic relationships to the Zhuang and Miao groups; both populations had a high degree of genetic differentiation from most Turkic-speaking populations. Aforementioned results suggested that the 30 autosomal InDels are highly polymorphic and informative, which are suitable for human identification and population genetics. 相似文献
16.
Santa Catarina is a small, developed, and relatively safe state in South Brazil. Despite having positive social economic indicators, it still faces multiple challenges regarding forensic practices for personal identification. The objective of this paper is to discuss the recent advances and current challenges in the region, from the perspectives of anthropological and dental postmortem human identification, missing persons, and disaster victim identification (DVI) from 2019 to 2021. The recent creation of a Forensic Anthropology Sector (SAF) in the state’s official forensic institution (Polícia Científica—PCI) has significantly improved identification of unidentified remains and optimised resources available for DNA analysis. However, SAF is still quite understaffed, which negatively affects the recovery of skeletal material, its preparation, and the time needed for filing reports. Santa Catarina has passed legislation for missing persons in 2015, 4 years prior to the sanction of federal laws implementing the national policy for the disappeared. Nonetheless, a lack of integration between stakeholders remains a problem that PCI has tried to circumvent with the Conecta Programme, a multidisciplinary and integrated initiative between families of the missing persons, police agencies, and the Public Ministry. The programme aims to collect not only reference DNA samples, but also relevant anthropological and dental data. It also offers facial progression services in cases of disappearances that occurred many years ago. Despite a history of disasters in the state, PCI still needs to implement international DVI standards at an institutional level. Recent training on Phase 1 DVI procedures, integrated with other responding institutions, indicates better preparation for future disasters. There are many challenges ahead for Santa Catarina’s forensic institution and professionals that have yet to be addressed, but the overall situation on routine personal identification, missing persons initiatives, and DVI has improved over the last 2 years. 相似文献
17.
Evelyne Pessoa Soriano Maria do Socorro Dantas de Araújo Francisca de Assis Nascimento Pereira Francisca Divina Silveira de Melo Cristiane Helena da Silva Barbosa Freire Marcus Vitor Diniz de Carvalho 《法庭科学研究（英文）》2022,7(4):637
Several decomposed body parts were received for examination by the Forensic Anthropology section of the Medico-legal Institute of João Pessoa, Paraíba, Brazil. The portions of the lower and upper limbs, ribs, vertebrae, and a skull were thoroughly examined. The biological profile indicated a male individual with an estimated age range between 23 and 57 years and a mean age of 35.2 years (SD = 9.4; phase IV, Suchey-Brooks). The skeleton showed injuries caused by sharp force and sharp-blunt force trauma that affected all body segments. Macroscopically, the lesions are mainly in the diaphyseal segments of the long bones, sacrum, pelvis, mandible, maxilla, scapulae, sternum, vertebrae, the distal epiphysis of the left fibula, and the distal epiphysis of the left tibia displayed characteristics compatible with injuries produced perimortem. It was not possible to determine the cause of death. DNA analysis resulted in a positive identification. Because of common difficulties faced in forensic practice, it is often not possible for forensic anthropologists to go to the crime scene, X-ray or body scanners are frequently unavailable, and the victim’s medical and/or dental records are sometimes absent. These difficulties make identification ultimately depend on genetic analysis, which is more time-consuming than other identification methods. Despite this, bone fragment examination in dismemberment cases is a complex task. Forensic Anthropology can shed light on cases involving the identification of dismembered remains, which are challenging because of the number of traumatic injuries, as well as different injury patterns, on bones. 相似文献
18.
Marcos Paulo Salles Machado Andrei de Souza Santos Gabriela Graa Luciana Lima Ricardo Campos Barcellos 《法庭科学研究（英文）》2022,7(4):633
It is a well-established fact that forensic anthropology has been extremely helpful in identifying thermally altered human remains, especially in recent years. With the improvement of new analytical techniques, the identification success rate has also been increased. With the help of statistical analysis, combined with analytical anthropological methods, the Forensic Anthropology Service of the Afranio Peixoto Medico-Legal Institute succeeded in identifying a charred body, with a margin of error of approximately 1:500 million to be obtained. 相似文献
19.
Forensic haplotype analysis of the male Y chromosome is currently used to establish the number of male donors in sexual assaults, the number of male bleeders in blood pattern analysis, and for ancestry correlation to genetic founder populations in biogeographic studies. In forensic laboratory applications, its primary use is for DNA profile generation with trace amounts of male DNA in the presence of excess female DNA (e.g. spermatozoa identification, male component of fingernail scrapings). Our study supports the potential use of the Y chromosome in a “dragnet” approach (most haplotypes are unique) similar to that described by Kayser in 2017 for solving a cold case sex assault and homicide in The Netherlands. Our study also researched the potential for the identification of an ancestral Irish genetic “footprint” linked to surname O’Brien and identified multiple founder group origins in Ireland and England as well as three samples with the Dal Riata (a Gaelic overkingdom) ancestral haplotype. This study indicates correlation to ancestral Irish ancestry by haplotype but not conclusively to the O’Brien surname. 相似文献
20.
Emiliano Giardina Irene Predazzi Ilenia Pietrangeli Paola Asili Patrizio Marsala Luciano Gabriele Claudio Pipolo Omero Ricci Claudia Martone Aldo Spinella Giuseppe Novelli 《Forensic Science International: Genetics Supplement Series》2007,1(3-4):e1-e3
Allele frequencies for 16 previously described autosomal SNPs were tested in 1020 unrelated individuals originating from three different continents (Africa, Asia and Europe). The populations analyzed included Africans from Benin Gulf (180), Asians from Mongolia (160) and Europeans from Italy (680). 相似文献