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1.
Katherine Butler Gettings;Andreas Tillmar;Kimberly Sturk-Andreaggi;Charla Marshall; 《Journal of forensic sciences》2024,69(5):1546-1557
In mass disaster events, forensic DNA laboratories may be called upon to quickly pivot their operations toward identifying bodies and reuniting remains with family members. Ideally, laboratories have considered this possibility in advance and have a plan in place. Compared with traditional short tandem repeat (STR) typing, single nucleotide polymorphisms (SNPs) may be better suited to these disaster victim identification (DVI) scenarios due to their small genomic target size, resulting in an improved success rate in degraded DNA samples. As the landscape of technology has shifted toward DNA sequencing, many forensic laboratories now have benchtop instruments available for massively parallel sequencing (MPS), facilitating this operational pivot from routine forensic STR casework to DVI SNP typing. Herein, we present the commercially available SNP sequencing assays amenable to DVI, we use data simulations to explore the potential for kinship prediction from SNP panels of varying sizes, and we give an example DVI scenario as context for presenting the matrix of considerations: kinship predictive potential, cost, and throughput of current SNP assay options. This information is intended to assist laboratories in choosing a SNP system for disaster preparedness. 相似文献
2.
Comparison of antemortem and postmortem dental records is a leading method of victim identification, especially for incidents involving a large number of decedents. This process may be expedited with computer software that provides a ranked list of best possible matches. This study provides a comparison of the most commonly used conventional coding and sorting algorithms used in the United States (WinID3) with a simplified coding format that utilizes an optimized sorting algorithm. The simplified system consists of seven basic codes and utilizes an optimized algorithm based largely on the percentage of matches. To perform this research, a large reference database of approximately 50,000 antemortem and postmortem records was created. For most disaster scenarios, the proposed simplified codes, paired with the optimized algorithm, performed better than WinID3 which uses more complex codes. The detailed coding system does show better performance with extremely large numbers of records and/or significant body fragmentation. 相似文献
3.
Bryan T. Johnson; 《Journal of forensic sciences》2024,69(5):1681-1689
Postmortem fingerprinting is the fastest and most reliable way to scientifically identify unknown decedents from mass fatality incidents. In disaster victim identification (DVI), fingerprints, DNA, and dental examinations are the three primary methods of identification. Additional secondary identifiers such as comparative medical radiography, scars, marks, and tattoos can also be used if viable antemortem information can be located. As DNA technology continues to evolve, RAPID DNA may now identify a profile within 90 min if the remains are not degraded or comingled. When there are true unknowns, however, there is usually no DNA, dental, or medical records to retrieve for a comparison without a tentative identity. It is imperative to understand how to properly collect postmortem prints following various postmortem changes, which databases are available to search against, and what additional resources are available prior to any such event occurring. With advances in technology, automated fingerprint searching is faster than ever before. Mobile devices can now search all the major databases within a matter of minutes from a cell phone or tablet utilizing a peripheral fingerprint scanner. Preliminary research into the application of WD-40® when utilizing optical-capacitive scanners has shown to greatly increase the efficacy of capturing usable fingerprints. This additional step allows for the digital capture of fingerprints in even complicated settings, both in daily cases and DVI incidents. This article outlines all the available ways to obtain postmortem fingerprints in complex cases, and how they can be applied to efficiently and effectively used in the DVI process. 相似文献
4.
Two onboard crew members lost their lives in the fatal Makalu Air Cessna Grand Caravan 208B domestic cargo flight crash on May 16, 2018. The Disaster Victim Identification (DVI) procedure comprises external examination, photography, DNA collection, fingerprint collection, postmortem examination, antemortem information collection from the family members, and reconciliation. The major challenge of this operation was dealing with cognitive bias. The antemortem dental information of one of the deceased was revealed to the forensic experts just before the postmortem examination. This influenced the testing strategies. There was a tendency to neglect the complete dental examination presuming the identification was established. Later, during a thorough examination, the forensic odontologist realised that the initial decision was erroneous. Furthermore, there are few experience-based resources available to resolve cognitive bias issues. The authors begin by summarising complicated operations in which they have been involved, followed by a discussion of the key sources of cognitive bias along with the solution to resolve these issues in DVI preparedness planning. 相似文献
5.
6.
Analysis of DNA from burnt bone fragment is the very hard work for the human identification in forensic casework. In general, cremated bone with an artificial damage is more difficult to get an intact DNA than a singed one because of the chemical and biological drastic changes such as protein denaturation and destruction. In this study, we pursue the best technical approach for the minimal damage and the contamination of DNA from other factors in the preconditioning and the extraction process based on over 70 years old Korean War victim skeletal that was burnt and buried in Korean Peninsula. First of all, we removed the pollutant and the dust from the burnt bones using dental instruments, and then incubated with EDTA buffer at 25 ℃ to remove inhibitors such as calcium and mineral. In order to compare the DNA preservation ability between a pellet and a supernatant, samples are repeatedly tested to collect washed EDTA buffer several times to separate. Each of isolated materials is secondly cleaned with the organic extraction method using phenol and analyzed mtDNA sequence with the in-house method for the ancestry assay. The better discrimination ability was appeared in the supernatant than the pellet. Nevertheless, many of the forensic geneticists use a powdering method for getting more DNA, we applied EDTA buffer in the preconditioning step to eliminate every contamination. As a result, the contamination factor was efficiently removed and the ancestry was estimated as per the written information. Consequently, cremated bone is identified to belong in the D4 mtDNA haplogroup which is commonly reported in ethnic groups in Asia especially Korea. This is a preliminary study of a human identification over an ancestry analysis to give information against a mass disaster in a future. Through a higher process optimization and better analytical methods toward more remains, which are genetically difficult to analyze, will support to examine the identity of the post cremated remains. 相似文献
7.
Morgan Eaton;M. Shane Woolf;Samyuktha Pemmasani;Triniti Turner;Janina Golob Deeb;Tracey Dawson Green; 《Journal of forensic sciences》2024,69(6):2197-2208
Disaster victim identification (DVI) refers to the forensic identification of unknown individuals following a mass disaster event. Human dental structures can contain viable DNA sources when other soft tissues are compromised. However, labor-intensive sample preparation performed by extensively trained personnel is needed to expose the nuclear material for traditional forensic DNA workflows. With this in mind, we evaluated two simplified sample preparation protocols for processing tooth samples using either a conventional forensic DNA workflow or the Applied Biosystems® RapidHIT™ ID instrument. Briefly, sample sets for both protocols included 10 deciduous teeth that were cleaned prior to either fragmentation with a claw hammer (for RapidHIT™ ID processing) or fine-powder pulverization with a consumer-grade coffee grinder (for traditional workflows). The average percentage of expected STR alleles that were detected above analytical threshold for these tooth samples were comparable between methods: RapidHIT™ ID = 99.0% and GlobalFiler™ = 99.8%. Average intralocus heterozygote peak height ratios (PHRs) were comparable: RapidHIT™ ID = 0.80 and GlobalFiler™ = 0.86. Importantly, 9 of 10 samples analyzed via the RapidHIT™ ID required analyst review for flagged artifact peaks and quality issues. Across all profiles, 91% of alleles passed quality metrics for the RapidHIT™ workflow versus 100% for conventional GlobalFiler™ analysis. Collectively, these results suggest that quick, low-tech tooth sample fragmentation followed by analysis with the RapidHIT™ ID instrument can produce complete STR profiles from aged tooth samples. Future studies should include larger samples sets, more challenging tooth samples, and further simplification of sample preparation to enable field-forward, on-scene DVI. 相似文献
8.
Marcos Paulo Salles Machado Andrei de Souza Santos Gabriela Graa Luciana Lima Ricardo Campos Barcellos 《法庭科学研究（英文）》2022,7(4):633
It is a well-established fact that forensic anthropology has been extremely helpful in identifying thermally altered human remains, especially in recent years. With the improvement of new analytical techniques, the identification success rate has also been increased. With the help of statistical analysis, combined with analytical anthropological methods, the Forensic Anthropology Service of the Afranio Peixoto Medico-Legal Institute succeeded in identifying a charred body, with a margin of error of approximately 1:500 million to be obtained. 相似文献
9.
Evelyne Pessoa Soriano Maria do Socorro Dantas de Araújo Francisca de Assis Nascimento Pereira Francisca Divina Silveira de Melo Cristiane Helena da Silva Barbosa Freire Marcus Vitor Diniz de Carvalho 《法庭科学研究（英文）》2022,7(4):637
Several decomposed body parts were received for examination by the Forensic Anthropology section of the Medico-legal Institute of João Pessoa, Paraíba, Brazil. The portions of the lower and upper limbs, ribs, vertebrae, and a skull were thoroughly examined. The biological profile indicated a male individual with an estimated age range between 23 and 57 years and a mean age of 35.2 years (SD = 9.4; phase IV, Suchey-Brooks). The skeleton showed injuries caused by sharp force and sharp-blunt force trauma that affected all body segments. Macroscopically, the lesions are mainly in the diaphyseal segments of the long bones, sacrum, pelvis, mandible, maxilla, scapulae, sternum, vertebrae, the distal epiphysis of the left fibula, and the distal epiphysis of the left tibia displayed characteristics compatible with injuries produced perimortem. It was not possible to determine the cause of death. DNA analysis resulted in a positive identification. Because of common difficulties faced in forensic practice, it is often not possible for forensic anthropologists to go to the crime scene, X-ray or body scanners are frequently unavailable, and the victim’s medical and/or dental records are sometimes absent. These difficulties make identification ultimately depend on genetic analysis, which is more time-consuming than other identification methods. Despite this, bone fragment examination in dismemberment cases is a complex task. Forensic Anthropology can shed light on cases involving the identification of dismembered remains, which are challenging because of the number of traumatic injuries, as well as different injury patterns, on bones. 相似文献
10.
Yara Vieira Lemos Luciene Menrique Corradi Melina Calmon Silva Lorena de Oliveira Couto Giovanna Hooper Bittencourt Alexander Santos Dionísio Adriana Zatti Lima Mrcio Alberto Cardoso Eugnia Cunha 《法庭科学研究（英文）》2022,7(4):628
This article presents a forensic case of an unusual illegal cremation of a person using a process colloquially known as the “microwave oven” practice in Brazil. The microwave process involves two actions: placing the victim in a tire stack and then setting the structure on fire using flammable substances to accelerate the progression. A similar practice, identified as “necklacing”, has also been reported in other countries such as South Africa. This report presents a case of microwave oven cremation of a body found in a rural area of Minas Gerais, Brazil. The forensic work helped determine the biological profile and identity of the victim using radiological comparisons. Although the microwave oven cremation practice is rare, it can impose challenges for investigators. Therefore, fully understanding this practice can be helpful to the academic and forensic communities. 相似文献
11.
Fingerprints are frequently encountered during both civil and criminal investigations. Fingerprints possess numerous characteristics that assist with personal identification. Determining the hand of origin (right or left) for an individual fingerprint would help reduce investigation time and potentially eliminate certain suspects. In this study, we collected a total of 2 900 single digit fingerprints from 290 individuals, and the whorl axis slant was examined in the 743 whorl pattern fingerprints (385 from the right hand and 358 from the left hand). A slant towards the right side was present in 81.82% of samples from the right hand, while a slant towards the left side was observed in 80.73% of samples from the left hand. After applying a chi-square test to the dataset, the results were found to be statistically significant for the determination of hand origin. Our results suggest that the whorl axis slant in a fingerprint is indicative of hand origin (right or left).
Key points
- Single digit fingerprints with whorl pattern were analyzed.
- Whorl “axis slant” was used to determine the hand origin.
- Right axis slant would indicate the right hand of the print.
- Left axis slant would indicate the left hand of the print.
12.
The deaths of 10 bushfire (brushfire) victims (aged 2-59 years; M/F 1:1) from the files of Forensic Science SA in Adelaide, South Australia, over an 8-year period (January 2002 to December 2009) are reported. Nine of the victims were found in or near motor vehicles. Death was attributed to incineration (N = 5), trauma from bushfire-related vehicle crashes (N = 2), inhalation of products of combustion with hyperthermia (N = 1), inhalation of products of combustion (N = 1), and undetermined (N = 1). Death scenes covered large areas and involved many victims. Loss of infrastructure and closure of local roads owing to debris limited access and made the finding of bodies difficult. Bodies in such fires may be exposed to the damaging effects of weather and animal predation. Heat damage hindered pathological assessment with resultant delays in identification. Assessment of antemortem injuries and determination of causes of death were also complicated by the condition of some of the bodies. 相似文献
13.
Abstract: Mass fatalities can present the forensic anthropologist and forensic pathologist with a different set of challenges to those presented by a single fatality. To date radiography has played an important role in the disaster victim identification (DVI) process. The aim of this paper is to highlight the benefits of applying computed tomography (CT) technology to the DVI process. The paper begins by reviewing the extent to which sophisticated imaging techniques, specifically CT, have been increasingly used to assist in the analysis of deceased individuals. A small scale case study is then presented which describes aspects of the DVI process following a recent Australian aviation disaster involving two individuals. Having grided the scene of the disaster, a total of 41 bags of heavily disrupted human remains were collected. A postmortem examination was subsequently undertaken. Analysis of the CT images of all body parts ( n = 162) made it possible not only to identify and side differentially preserved skeletal elements which were anatomically unrecognizable in the heavily disrupted body masses, but also to observe and record useful identifying features such as surgical implants. In this case the role of the forensic anthropologist and CT technology were paramount in facilitating a quick identification, and subsequently, an effective and timely reconciliation, of body parts. Although this case study is small scale, it illustrates the enormous potential for CT imaging to complement the existing DVI process. 相似文献
14.
104耳鼓膜穿孔耳内窥镜电脑成像系统分析 总被引：7，自引：0，他引：7
目的 研究耳内窥镜电脑成像系统在鼓膜穿孔法医学鉴定中的价值。方法 用耳内窥镜电脑成像系统,观察已明确诊断97例患者的104耳鼓膜穿孔,并分析不同性质鼓膜穿孔特点。结果 在97例104耳鼓膜穿孔中,外伤性鼓膜穿孔70耳,左44耳,右26耳,均为单侧,以三角形、梭形、裂隙形为主,多发生在前下象限;炎症性鼓膜穿孔 34耳,左19耳、右15耳,单侧20例,双侧7例,以圆形、类圆形为主,多发生在后下象限。结论 两种鼓膜穿孔在耳别、形态、部位等指标上有明显差异;用耳内窥镜电脑成像系统观察鼓膜穿孔,其结果客观、准确,易保存,可为鼓膜穿孔的法医学鉴定提供依据。 相似文献
15.
Diseases not only bring troubles to people’s body functions and mind but also influence the appearances and behaviours of human beings. Similarly, we can analyse the diseases from people’s appearances and behaviours and use the personal medical history for human identification. In this article, medical indicators presented in abnormal changes of human appearances and behaviours caused by physiological or psychological diseases were introduced, and were applied in the field of forensic identification of human images, which we called medical forensic identification of human images (mFIHI). The proposed method analysed the people’s medical signs by studying the appearance and behaviour characteristics depicted in images or videos, and made a comparative examination between the medical indicators of the questioned human images and the corresponding signs or medical history of suspects. Through a conformity and difference analysis on medical indicators and their indicated diseases, it would provide an important information for human identification from images or videos. A case study was carried out to demonstrate and verify the feasibility of the proposed method of mFIHI, and our results showed that it would be important contents and angles for forensic expert manual examination in forensic human image identification. 相似文献
16.
《法庭科学研究（英文）》2020,(1):中插9,74-84,中插10-中插17
Examination of a person who has been a victim of a physical or sexual assault may be very important for upcoming legal proceedings.In the context of a clinical ... 相似文献
17.
Aluisio Trindade Filho 《法庭科学研究（英文）》2022,7(4):623
When human remains are examined, three questions always need to be answered: who is the deceased, what was the cause of death, and when did the death occur, the former question being the most relevant. The identification of half or fully skeletonized human remains is a complex process and always requires the use of methods that allow individualization beyond any reasonable doubt. However, no matter how vigorous the search for identification, this is not always achieved. Here, the author presents two cases in which identification was exhaustively attempted but not achieved despite the existence of an osteo implanted device in one case and the presence of documents in the other. In one case, we could not find a potential identity for the deceased, while in the other we found a possible identity but not a family member to provide antemortem data to confirm it. Although the scientific literature tends to favour the publication of cases with favourable outcomes, one should also learn from failures, which is the reason why the author decided to publish his unsuccessful experiences. The reasons for the failures are discussed, as well as methodological improvements for future cases. 相似文献
18.
In December 2017, a decomposed unidentified body was found near the river Tronto in Teramo, Italy. The corpse was found without any identifying documents or specific personal belongings, except for a packet of cigarettes. The medical examiner determined a gastric perforation from the intake of hydrochloric acid to be the cause of death. A jar of muriatic acid found near the body led to suicide being considered the manner of death. The Penal Court in Teramo appointed two forensic odontologists to complete the postmortem assessment and collect dental data for personal identification. The corpse was found wearing a complete set of upper and lower dentures. The dental autopsy and 42 periapical X-ray images helped generate a biological profile of a man totally edentulous with upper and lower dentures, as well as an osteosynthesis with two plates and screws in the left ascending ramus of the mandible. In March 2018, the sister of a missing person reported the disappearance of her brother, and a presumptive identification was performed through visual recognition of the decomposed body. The sister confirmed the presence of two dentures and the location of the maxillo-facial surgery for the treatment of the fractured mandible. A complete dental autopsy was able to establish his identity without any DNA comparison needed. This case highlights the importance of performing a complete dental autopsy inclusive of dental radiographs, and its value in the identification of all unknown human remains even when totally edentulous.A complete dental autopsy should be performed in all cases of human identification.
Keypoints
- Despite a corpse being edentulous, a complete dental autopsy can still be useful.
- Dental radiographs, such as bitewings, periapical images, panoramic radiographs, and CT scans, are recommended in all identification autopsies.
19.
The estimation of stature is very important in forensic investigation, as it provides useful data that can narrow the pool of potentially matching identities. The purpose of this study was to develop formulae for the estimation of stature from footprint measurements in Bangladeshi adults. This study included 118 randomly selected men and 130 randomly selected women, all aged 18–50 years. From each participant, stature and six footprint measurements were taken by means of standard measurement techniques. Footprint measurements were found to be positively correlated with stature. Stature was estimated by using linear regression equations. The right T1 length in men (R: +0.587, R2: 0.345) and the right T2 length in women (R: +0.506, R2: 0.256) were the most reliable individual estimators of stature. However, when data were combined for both sexes, the right T2 length was identified as the most reliable estimator of stature, with higher values of R (+0.792) and R2 (0.627). In conclusion, human stature can be successfully estimated by using footprint measurements; this finding can be applied in forensic research and investigation. 相似文献
20.
Raymond G. Miller; 《Journal of forensic sciences》2024,69(5):1630-1636
This paper reviews MFI's from a historical perspective commencing with DVI in the late 20th century. For this paper, this era, 1970–90s is designated as the early modern period. As DVI by DNA analysis is introduced into the process, in the beginning of the mid-1990s, or late modern period, a shift in ID modality usage is noted. A statistical analysis of the primary identification (ID) methods established that dental identification was the majority identifier, or gold standard, in the early modern era. Although primarily viewed from a United States (US) perspective, referenced international incidents parallel the incidents investigated by US authorities. The introduction of DNA demarcated the early from the late modern era. Through research, development, and application this highly discriminating ID method would effectively, surpass dental ID as the gold standard into the late modern era. DNA ID would eventually overcome early criticism regarding cost and time consumption. In the MFI's discussed, the discriminating accuracy of DNA, when referenced against the dental identifications, validated the reliability of dental ID. Errors will be significantly minimized through confirmatory reconciliation by more than one ID method. In conclusion despite increased usage of DNA, dental ID has not been eliminated and remains a major contributor to DVI. Dental ID continues to develop through increased application of advanced imaging technology. Despite DNA's rapid advancement and application to DVI, the multidisciplinary approach to scientific identification should remain in the near future. Therefore, comparative dental ID will remain an important and reliable contributor to DVI. 相似文献