Recovery of decomposed and skeletal human remains in the "Green River Murder" Investigation. Implications for medical examiner/coroner and police

The Green River Murder Investigation in King County, Washington, is currently the longest active serial murder investigation in U.S. history. During its course, over 26 separate scenes with from one to five victims each have been processed. The experience of the authors is presented in order to acquaint other agencies with techniques of outdoor scene processing that have evolved during recovery of remains from Green River and other skeletal cases.     

Forensic Use of the Piracatinga Fish (Callophysius macropterus) to Locate and Identify Human Remains Retrieved From the Amazon River

Piracatinga (Callophysius macropterus) are a type of bottom feeder catfish encountered in the Amazon River and its tributaries. We report two cases in which human remains were first located based on a characteristic circular distortion of the surface of the river that the Piracatinga make while they feed. Human skin samples of one of the victims recovered from the Piracatinga digestive tract were subjected to mitochondrial DNA analysis that allowed identification of the body of Case 1; the family recognized body parts of Case 2. Importantly, the location of human body parts and their identification based on DNA analysis enabled the respective families to obtain a death certificate expeditiously in the absence of identifiable remains—a process that normally requires 5 years under current Brazilian law, and in the absence of closure, imposes severe emotional stress on the family of the deceased.     

The use of cadaver dogs in locating scattered, scavenged human remains: preliminary field test results

Specially trained air scent detection canines (Canis familiaris) are commonly used by law enforcement to detect narcotics, explosives or contraband, and by fire investigators to detect the presence of accelerants. Dogs are also used by police, military, and civilian groups to locate lost or missing persons, as well as victims of natural or mass disasters. A further subspecialty is "cadaver" searching, or the use of canines to locate buried or concealed human remains. Recent forensic investigations in central Alberta demonstrated that the use of cadaver dogs could be expanded to include locating partial, scattered human remains dispersed by repeated animal scavenging. Eight dog-and-handler teams participated in a two-month training program using human and animal remains in various stages of decay as scent sources. Ten blind field tests were then conducted which simulated actual search conditions. Recovery rates ranged between 57% and 100%, indicating that properly trained cadaver dogs can make significant contributions in the location and recovery of scattered human remains.     

Missing in Amazonian Jungle: A Case Report of Skeletal Evidence for Dismemberment*

Abstract: This case study presents the results of the recovery and analysis of three sets of disarticulated and incomplete human remains found in Ecuador, within the Amazonian jungle. Recovered body parts sustained extensive sharp force trauma situated on different aspect of the skeleton. The anthropological examination (bone reassembly, biological profile) was followed by a detailed analysis of cut marks, including a basic experimental study on pig bones to demonstrate that dismemberment may have occurred within a certain amount of time after death. Despite the location (deep into the Amazonian jungle) and the perpetrator’s actions (dismemberment and dispersion of body parts in a river), forensic work both on the field and in laboratory allowed identification of the victims and the reconstruction of the sequence of events.     

Refining Stable Oxygen and Hydrogen Isoscapes for the Identification of Human Remains in Mississippi,

Isoscape refinement is an essential component for accurately predicting region‐of‐origin in forensic investigations involving isotope analysis of unidentified human remains. Stable oxygen (δ¹⁸O) and hydrogen (δ²H) isotopes were measured from 57 tap water samples collected across Mississippi to model refined isoscapes for the state. A tap water conversion equation, δ¹⁸O_tw=1.64 δ¹⁸Op?31.35, was developed for the southeastern USA to test the prediction accuracy of the δ¹⁸O_tw isoscape using individuals with known residential histories. A local Mississippi resident (USAFA‐134) was assigned with 90% probability to the correct region‐of‐origin reported by the participant. Assignments for Georgia residents (USAFA‐118 and USAFA‐205) had variable results, predicting USAFA‐118 from Mississippi and USAFA‐205 as a nonlocal resident. Stable isotope values often overlap geographically and a multi‐isotope approach should be used when narrowing region(s)‐of‐origin(s). This study demonstrates the utility of refining isoscapes and the importance of tissue calibration in prediction assignments of human remains.     

Use of a Gelatin‐based Consolidant to Preserve Thermally‐Altered Skeletal Remains,

Thermally altered skeletal remains can be very fragile and fragmented and are typically further fragmented or even destroyed when handled; recovery of such remains from a scene can therefore be extremely challenging. There are few recommendations and no generally accepted practices for preserving burned bone for recovery and transport. Here, we test whether the application of a gelatin‐based consolidant at the scene can preserve thermally altered bone in the condition and relative anatomical position in which it was discovered. A solution of Knox^® brand gelatin and water was applied to burned pig mandibles using a spray bottle. Qualitative and quantitative analysis indicates that the application of the consolidant significantly decreased fragmentation as compared to untreated controls (p < 0.05), with most of the treated mandibles remaining completely intact after recovery and transport to a secondary location. In addition to the effectiveness for preservation, the method is also easy to apply, inexpensive, and reversible.     

Investigation of Different Ways in Which the CODIS 7.0 May be Used in Mass Disaster Identification

DNA analysis is a key method for the identification of human remains in mass disasters. Reference samples from relatives may be used to identify missing persons by kinship analysis. Different methods of applying the CODIS in disaster victim identification (DVI) were investigated. Two searches were evaluated: (i) relating family relatives to a pedigree tree (FPT) and (ii) relating unidentified human remains to a pedigree tree (UPT). A joint pedigree likelihood ratio (JPLR) and rank were calculated for each search. Both searches were similar in average JPLR and rank. In exceptional cases, namely the existence of a mutation different from the CODIS model, a nonbiological father, a mistake in STR, or incorrect profile association, the UPT search returned one true rank, whereas the FPT search returned no results. This paper suggests a novel strategy to overcome these limitations and increase efficiency in conducting identification of mass disaster victims.     

Identification of decomposition volatile organic compounds from surface-deposited and submerged porcine remains

Cadaver dogs are routinely used internationally by police and civilian search organisations to locate human remains on land and in water, yet little is currently known about the volatile organic compounds (VOCs) that are released by a cadaver underwater; how this compares to those given off by a cadaver deposited on land; and ultimately, how this affects the detection of drowned victims by dogs. The aim of this study was to identify the VOCs released by whole porcine (Sus scrofa domesticus) cadavers deposited on the surface and submerged in water using solid phase microextraction gas chromatography mass spectrometry (SPME GC–MS) to ascertain if there are notable differences in decomposition odour depending on the deposition location.For the first time in the UK, the volatile organic compounds (VOCs) from the headspace of decomposing porcine cadavers deposited in both terrestrial and water environments have been detected and identified using SPME-GCMS, including thirteen new VOCs not previously detected from porcine cadavers. Distinct differences were found between the VOCs emitted by porcine cadavers in terrestrial and water environments. In total, seventy-four VOCs were identified from a variety of different chemical classes; carboxylic acids, alcohols, aromatics, aldehydes, ketones, hydrocarbons, esters, ethers, nitrogen compounds and sulphur compounds. Only forty-one VOCs were detected in the headspace of the submerged pigs with seventy detected in the headspace of the surface-deposited pigs. These deposition-dependent differences have important implications for the training of cadaver dogs in the UK. If dog training does not account for these depositional differences, there is potential for human remains to be missed.Whilst the specific odours that elicit a trained response from cadaver dogs remain unknown, this research means that recommendations can be made for the training of cadaver dogs to incorporate different depositions, to account for odour differences and mitigate the possibility of missed human remains operationally.     

The Identification of a Human Skull Recovered from an eBay Sale

Abstract:  A human skull seized by the State of Louisiana from an eBay sale is analyzed. Bioarchaeological analyses of age-at-death, sex, and population affinity suggest the individual represented by the skull was a middle-aged Native American female. The presence of intentional cranial modification independently supports the population affinity assessment while confounding the metric analyses. However, no further specificity as to population affinity could be inferred using existing methods and comparative databases. Sedimentological and palynological analyses were attempted to redress this impasse. The presence of fine-grained charcoal, abundant fungal remains, and small angular quartz grains suggestive of burial in loess, as well as the lack of pollen, pteridophyte spores, and microscopic algae, suggest a likely upland burial location from somewhere in the lower Mississippi Valley. The sedimentological and palynological analyses, while not conclusive, show promise for use in future affiliation analyses of human remains recovered during the course of forensic investigations. The results are reviewed within the broader context of the legal debate over the repatriation of human remains.     

Where are all the victims?

Research Summary The passage of new laws that criminalize the trafficking of persons for labor and sexual services has raised public awareness about the problem of human trafficking. In response, police must understand the problem, identify human trafficking victims, and make arrests. The numbers of victims identified to date, however, has paled in comparison with official estimates, which leads some to question the existence of a human trafficking problem. Missing from this debate is information about how frequently police encounter human trafficking and how well prepared officers are to handle these cases. Analyzing survey responses from a national sample of police agencies in the United States, we found that less than 10% of police agencies identified human trafficking cases from 2000 to 2006. Larger agencies were more likely to identify cases of human trafficking but the agency leader perception about the problem in their local communities as well as taking steps to prepare officers to identify and respond were the most important factors to increasing human trafficking identification by police. Policy Implications This study provides much needed information about why U.S. officials have identified so few human trafficking victims. By understanding how often and under what conditions police find, investigate, and prosecute cases of human trafficking we will be in a better position to identify and overcome barriers to police responses to trafficking and understand the limitations of official statistics about human trafficking. Data from a national survey also provide a baseline measure of police identification of human trafficking against which we can gauge the progress of future anti-trafficking efforts.     

Experiences of Stalking Victims and Requests for Help in Three European Countries. A Survey

Previous research by the Modena Group on Stalking (MGS) regarding awareness and recognition of stalking by police officers and general practitioners using case scenarios showed significant differences across three countries. It was also hypothesized that victims used different pathways when seeking help. To explore the experience of requesting help by victims of stalking in different European countries a detailed survey was completed by 391 victims of stalking in Belgium (n = 145), Italy (n = 126) and Slovenia (n = 120). The research confirmed the significant intensity and duration of stalking and psychological distress for victims. Victims used several pathways to survive stalking. Some victims reported that informal interventions they or family/friends implemented were effective, but the majority had to have recourse to helping agencies. Significant differences in help seeking behaviour were found between the three countries. In Belgium victims contacted the police, as well as other helping agencies, more often and more precociously than in Italy and Slovenia. Restriction orders against the stalker were considered the most effective intervention. Police were regarded as the least supportive agency, taking victims less seriously, and not being particularly effective at stopping the stalkers. Police were ranked after family/friends, lawyers and colleagues, with regards effectiveness, except in Belgium, where Police’s effectiveness was ranked second after lawyers. Stalking often requires the mobilization of multiple helping agencies by victims and it is important that victims are aware of what help is available to them and that agencies become more sensitive to their needs.     

Identification of serial homicide victims in the "Green River Murder" investigation

The Green River Murder Investigation in King County, Washington, is currently the longest and most active serial murder investigation in U.S. history. To date, little information has been reported on methods used in identification of serial murder victims. In this paper, various methods used in victim identification are reviewed and difficulties encountered during the course of the investigation are described. The experience of the authors is presented in order to acquaint other agencies with problems of victim identification associated with these serial murder victims and to provide key methods that may be useful in other such investigations.     

The application of geographic information systems and spatial analysis to assess dumped and subsequently scattered human remains

This study utilizes geographic information systems (GIS) and spatial analysis (SA) technology to address the problems associated with prediction of location and effective recovery of dumped and scattered human remains in Louisiana. The goals are to determine if a selective bias exists in Louisiana as to where and when human remains are dumped and to assess whether or not geographically specific patterns exist in the dispersal of human remains. We hypothesized that a positive relationship exists between postmortem interval (PMI) and dispersal distance, and that there are negative relationships between PMI and dispersal direction and between dispersal direction and distance. Our results indicate that, in Louisiana, remains are more often dumped in rural areas away from a structure, and are found within (1/4) mile of the nearest road. For Louisiana, no seasonal bias was found in the analysis of when remains are dumped. Furthermore, with the exception of the relationship between PMI and the shortest distance remains were dispersed, no geographically specific patterns were detected in the analyses of dispersal distance, dispersal direction, and PMI.     

Ankle joint injuries as a reconstruction parameter in car-to-pedestrian accidents

The study has assessed the usefulness of ankle joint injuries for deducing about the presumable location of a pedestrian in relation to a motor vehicle at the moment of collision. A statistically significant correlation was found between the direction of impact and the mechanism of ankle joint injuries. Such injuries were found in 113 of 317 pedestrian, fatal victims of road traffic accidents (36%). In the cases of impacts on the medial surface of the lower limb, the injuries associated with the supination mechanism were six times more frequent than the injuries associated with the pronation mechanism, while in the group of impacts on the limbs from the lateral side those connected with the pronation mechanism were four times more frequent. In victims hit from the back, the injuries connected with the plantarflexion mechanism were found almost four times more frequent than the dorsiflexion mechanism and in victims hit from the front in three of the five damaged joints the dorsiflexion component was observed (without any plantarflexion case). Less frequent "pronation injuries" in the limbs hit from the medial side and "supination injuries" in the limbs hit from the lateral side as well as "dorsiflexion injuries" in victims hit from the back predominantly occurred in impacts to the pelvic area, i.e. resulting from collisions with a van or lorry. Injuries of upper ankle joints are, thus, useful for reconstructing the presumable location of the pedestrian in relation to the vehicle at the moment of collision, especially, when the vehicle involved in the accident is known.     

Is there human DNA on cats

The transfer, persistence, prevalence, and recovery of DNA (DNA-TPPR) can be highly relevant in forensic investigations to evaluate the presence and/or actions of a person of interest (POI). Whilst the DNA-TPPR-related research has increased significantly over the last decade, there is a lack of data on companion animals and their relationship to human DNA transfer. Given the commonality of cats and dogs in households around the world, companion animals as receptors and vectors for DNA transfer can be highly relevant in cases involving animals as victims of a criminal offense, or cases requiring activity level evaluations. Samples were collected from an external area on the right side of 20 cats to determine the prevalence and sources of human DNA on this area. Preliminary data shows that human DNA is present on household cats, its source is mainly from household inhabitants. Further studies are required to elucidate the means and level of transfer of human DNA to and from cats and other household animals. This knowledge can be relevant to sample targeting in specific case circumstances and/or when considering possible means of the presence of a person’s DNA at the crime scene location.     

A microbiological test for the diagnosis of death by drowning

The present study was aimed at demonstrating the diffusion of sea water or freshwater into the bloodstream as a consequence of water aspiration due to drowning. The study was carried out on 42 study group subjects who died by drowning in salt water (20 cases) and freshwater (22 cases) and 30 control group subjects who died from causes other than drowning. For 25 cases we obtained water samples from the aquatic locations where the victims were found. The blood samples of study and control groups were analyzed to search for faecal coliforms (FC) and faecal streptococci (FS) bacteria. The presence of FC and FS was showed by the development of blue and red colonies, respectively. From left ventricular (LV) and right ventricular (RV) blood cultures of the 20 sea drowning victims we always isolated FS and FC, whereas 19 (95%) femoral arterial (FA) and 18 (90%) femoral venous (FV) blood cultures were positive for both faecal bacteria. Related to freshwater victims, LV blood cultures showed FS presence for all the 22 cases studied (100%) and FC presence for 20 cases (90.91%). Blood cultures from RV, FV, and FA showed various patterns of faecal bacteria presence. The analysis of 25 water samples from the aquatic locations where the victims were found showed the presence of FC and FS bacteria. Blood cultures from the 30 control subjects uniformly proved the absence of faecal bacteria.     

Ellis Kerley's service to the military.

The purpose of this paper is to present the contributions Ellis Kerley has made to the United States Department of Defense in the area of forensic anthropology. His service began at the end of the Korean War in the identification laboratory established in Kokura, Japan, under the direction of T.D. Stewart. Ellis developed his research on the quantification of cortical bone microstructure as a means of age estimation while at the Armed Forces Institute of Pathology. In December 1985, Ellis was asked by the Army to form a commission to evaluate the Central Identification Laboratory, HI (CILHI). This lab is tasked with the mission of search and recovery and identification of American personnel killed or listed as missing from past military conflicts. Ellis's team reviewed the identification process and documentation, the qualifications of lab personnel, the adequacy of facilities and equipment, and some questionable cases. Their written report was supplemented with testimony in front of congressional committees. Ellis served as scientific director of CILHI from 1987 until 1991. In addition to overseeing the daily case work, he led several missions to Vietnam to examine human remains and served as spokesman for the lab. His input helped the lab gain needed scientific credibility. Despite the frustrations of trying to identify human remains within the Army's casualty and memorial affairs system, Ellis always maintained the utmost dignity, compassion, and respect for the victims and their families.     

Personal fraud victims and their official responses to victimization

The bulk of the research on predatory crime focuses on characteristics, profiles, and patterns of offenders. This information is important, but from the perspective of police agencies, information about the victims of predatory crimes, including victims of white-collar predatory crimes, and how they officially respond to victimization is also important. Using survey data from a random sample of 400 residents of Knox County, Tennessee, we investigated the characteristics of victims of thirteen different types of fraud. After dividing individuals into categories of non-victims, one-time victims, and repeat victims, we develop profiles of personal fraud victims by examining differences in key demographic and offense characteristics. Next, we investigate the official reporting behavior of victims. The results are instructive for police agencies as they highlight common characteristics of fraud victims and suggest methods for encouraging fraud victims to report their victimization to police.     

Radiological, forensic, and anthropological studies of a concrete block containing bones

Multidisciplinary forensic, anthropological, and radiological studies of bone fragments encased in a concrete block were carried out to determine whether or not the bones were human. Multislice computed tomography (MSCT) investigation was performed before the bones were removed from the concrete. MSCT study pinpointed the location of the bone fragments within the concrete block, which was helpful for their extraction and recovery, and identified most of their types and nature. Osteological study on dry bones provided more accurate identification of the bones and of their side. According to both methods, the human skeletal remains were compatible with those of a child, aged 8-13 years old, with a minimum height of 128 cm. Neither investigation identified sex or racial phenotype. Both studies identified the skeletal remains as consisting of two animal and five human bones. Furthermore, both methods revealed that the concrete completely encased bones, suggesting a secondary burial.     

Securing the Border in Bosnia and Herzegovina: an Exploratory Analysis of the Impact of Training on Officers’ Knowledge and Experiences Related to Sex Trafficking

Across the globe law enforcement agencies are providing training specific to human trafficking in an effort to educate officers about trafficking indicators, techniques for evidence collection, and the provision of culturally sensitive and victim-centered assistance to trafficking victims. The effectiveness of said training, however, remains an understudied area. The primary goal of this study is to examine the influence training programs have on police officers’ knowledge and experiences related to sex trafficking. Utilizing self-report data from 363 border patrol agents in Bosnia and Herzegovina, a series of statistical analyses finds support for the hypotheses that trained officers will have a better understanding of sex trafficking indicators and field investigation techniques as well as more experience with sex trafficking cases. Somewhat unexpectedly, the results indicate that the vast majority of officers, regardless of training receipt, recognized a need for ongoing training and support. Implications of these findings will be discussed.