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用新设计引物调查武汉汉族Penta D和Penta E基因座遗传多态性 总被引:1,自引:0,他引:1
目的 研究PentaD和PentaE基因座分型引物设计 ,调查PentaD和PentaE基因座在武汉汉族人群中的遗传多态性。方法 用重新设计的PentaD和PentaE基因座分型引物 ,采用热启动PCR和PAGE技术对 2 81名武汉地区汉族无关个体进行分型调查 ,并将其分型结果与Promega公司的PowerPlexTM16系统荧光标记复合扩增试剂盒分型结果进行比较。结果 新设计引物扩增产物的片段大小范围分别为 15 3~ 198bp和 10 7~ 2 12bp ,其分型结果与PowerPlexTM16系统的分型结果完全一致 ,且用银染法检测的灵敏度显著提高 (由 0 5ng提高到 0 2ng)。PentaD和PentaE基因座在武汉汉族群体分别检出 10个和 2 1个等位基因 ,其基因型频率分布均符合Hardy Weinberg平衡。家系调查证实了其等位基因的传递符合孟德尔遗传规律。两基因座的个体识别能力 (DP)分别为 0 92 62、 0 9860 ,非父排除率 (PE)分别为 0 665 1、 0 83 2 5。结论 新设计引物用于PentaD和PentaE基因座的分型检测准确可靠 ,两基因座多态性程度高 ,在法医学个人识别和亲子鉴定中具有使用价值。 相似文献
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微卫星(STR)基因座常根据串联重复单元的核苷酸数进行分类。其中五核苷酸重复序列(Pentanucleotide)是指串联重复单元为五核苷酸的STR基因座,其结构比四核苷酸重复序列更加稳定[1]。四核苷酸序列基因座是法医DNA分析最常用的基因座,而五核苷酸序列基因座在当前法医学所采用的商品化的试剂盒中仅有Penta E、Penta D(Promega,USA),所以存在很大的研究空间。本文选取Penta B、Penta E和Penta C(基因座信息见表1)三个STR基因座,在北京地区汉族人群中,随机抽取203名无关个体进行多态性调查。表1 Penta B、E、C基因座信息基因座基因… 相似文献
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1案例资料
1.1案件简介
案例1某年10月下旬,市郊花坛内发现一名男婴,经走访是王某夫妇所遗弃,采集王某夫妇和婴儿纸质血样要求DNA检验,结果发现男婴D21S11、Penta D基因座STR分型检出三等位基因。
案例2某年4月7日上午,城区文化广场附近小溪岸边发现一具女尸,其尸体东南侧50m范围内有滴落状血迹,DNA检验系一女性所留,但Penta D基因座检出三等位基因,采集嫌疑对象和其父母血样进行DNA检验。 相似文献
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本文采用DNATyperTM15荧光复合扩增系统对江苏地区汉族人群Penta E和D6S1043基因座的遗传多态性进行调查,为个体识别、亲权鉴定及DNA数据库建设提供群体遗传学基础数据。1材料与方法1.1样本410份江苏地区汉族无关个体血样由作者实验室日常办案积累。1.2方法样本参照行业标准GA/T383-2002,使用硅珠法提取模板DNA。 相似文献
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Copper BK 《Journal of health law》2007,40(1):65-105
In an era filled with fears of bioterrorism, Congress approved the Public Readiness and Emergency Preparedness Act (PREPA) to encourage development of vaccines and other countermeasures. By providing pharmaceutical manufacturers with protection from liability for potential side effects, Congress has attempted to motivate manufacturers to produce a national stockpile of countermeasures. As part of PREPA, the government established a compensatory system intended to provide compensation to persons injured by countermeasures used during a public health emergency. Although the Act provides for a compensation fund, it fails to allocate monies for that fund. Thus, in the absence of further congressional action, PREPA will not provide compensation to those injured by countermeasures. Failing to assure the American public of a compensation program constitutes bad public policy and risks inspiring potential vaccinees to refuse necessary drugs. Additionally, arguments as to the constitutionality of the Act exist should Congress fail to adequately fund the program, and the existence of those arguments undermines the purpose of the Act--namely to assure pharmaceutical manufacturers that they will not be sued into oblivion should they attempt to aid national pandemic protection. In addition to detailing both the Act and the statutory precedent for congressional attempts to spur biodefense, this Article addresses important issues of healthcare, tort, and constitutional law that will continue to manifest themselves in this new era of bioterrorism. 相似文献
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ulfuzation,and discussed merits and defects of different kinds of desulfurizers.It indicated that the main technique of flue gas desulfurization in china is calcium way with calcic desulfurizer,and the most potential one is using ammonia as desulfurizer.Controlled sulfur pollution effectively and rejected seconda 相似文献
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Purpose
Despite an emerging body of research on the institutional behavior and adjustment of delinquent males, there exists little information on the incarceration experiences of female delinquents. The present study explored the incidence, prevalence, and determinants of institutional misconduct among a sample of 139 serious and violent delinquent females sentenced to state juvenile incarceration.Methods
Secondary data analysis was used for the present study. Data utilized were derived from information originally gathered by correctional staff during intake at a state Youth Correctional System (a pseudonym) and during an offender's entire incarceration through on-site diagnostic processes, staff observations, official records, and offender self-reports.Results
Members of the study sample engaged in roughly 700 incidents of major misconduct and more than 12,000 instances of minor institutional misconduct during their incarceration. Results from negative binomial regression models examining four different types of institutional misconduct revealed that age at commitment, offense type, mental health status, and gang affiliation were related to the expected rate of misconduct, although this varied by misconduct type.Conclusions
Institutionalization is not necessarily a period of desistance from offending for all delinquent girls. As institutional misconduct may impact post-release recidivism, it is important to identify and intervene with at-risk juveniles during periods of incarceration. 相似文献17.
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Stavros A. Drakopoulos Ioannis Theodossiou 《European Journal of Law and Economics》2016,41(3):641-656
The standard treatment of occupational risk in the labour market is conducted in terms of the theory of compensating wage differentials, the basic characteristic of which is that workers can fully estimate actual occupational risks. However, research in cognitive psychology, and recent advances in economic psychology, suggest that individuals consistently underestimate risks associated with accidents. In this paper, we discuss the case when the workers systematically underestimate job risks. After presenting the standard treatment of occupational risks, and of health and safety at work regulation, we then proceed to incorporate the idea of job risk underestimation. The paper discusses the types and impact of regulation on health and safety effort in a simple framework in which workers’ beliefs concerning accident risks also play a role. The paper shows that a particular type of regulatory intervention is necessary for the risk underestimating workers not to suffer a welfare loss. 相似文献
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Theories of democratic politics prize congruence between citizens’ preferences and their elected representatives’ actions in office. Elections are a critical means for achieving such policy congruence, providing voters the opportunity to chasten representatives who are out of step with constituent preferences and to reward the faithful. Do voters act this way? Recent studies based on observational data find they do, but these data are somewhat limited. We employ a survey experiment to estimate the extent to which information about policy congruence affects voters’ evaluations of representatives. We informed some subjects how often their member of Congress’s voting decisions match their own stated preferences on the same policies. We find that information about congruence enhances accountability by affecting constituent evaluations of representatives and may also affect citizens’ propensity to participate in upcoming elections. 相似文献