Photographic Analyses Using Skin Detail of the Hand: A Methodology and Evaluation,

Skin features have been employed by law enforcement agencies for suspect and victim identification. Comparisons of hand have arisen in casework where images have been submitted where a face was not present but a hand was visible. This research utilizes a collection of 128 hands from employees of the U.S. Army Criminal Investigation Laboratory to examine the frequency and distribution of skin detail on the dorsal surface of the hand. To assess the location of features, the hand was segmented into 14 regions using readily discernible anatomical landmarks. Overall, 2618 pigmented lesions and 92 scars or injuries were documented. When comparing the regions with one another, Regions 1–10 had fewer pigmented lesions than Regions 11–14. There was no pattern to the distribution of scars throughout the regions. The findings presented a foundation for one possible method that may differentiate hands based on the frequency and distribution of such features.     

The use of dental aging techniques in forensic odontological practice

Many studies have been published describing numerous techniques to determine the aging of individuals from dental tissues. However, few case reports demonstrate how such techniques can be employed by the forensic odontologist undertaking casework. Indeed, many of the techniques are highly complex and utilize equipment not generally available to odontologists outside of the university or hospital systems. This paper describes five cases where dental aging was used for identification in the absence of materials for any other techniques. In each case the Bang and Ramm method was employed using sectioned teeth. Teeth were sectioned, photographed, and scaled in image analysis software. Bang and Ramm equations were employed, and in each case an age was derived. The estimated age was supplied to the coroner, who was able to use the information to positively identify five individuals for whom no other identification system was feasible.     

An alternative method to screen for pepper spray residue

A method was developed to screen for pepper spray residue using instruments and methods other than those techniques commonly employed to analyze chemical residue (i.e.. gas chromatography mass spectrometry-GCMS or liquid chromatography mass spectrometry-LCMS). The method employed gas chromatography (GC), thin layer chromatography (TLC), and diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) to screen for dried pepper spray stains. Pepper sprays from nine different manufacturers were investigated. Capsaicin and dihydrocapsaicin were identified and unique IR reflectance spectra are presented. An additional five compounds were presumptively found. Results showed that a particular stain could be characterized as a pepper-based stain.     

Legal training and perceptions of Chief Housing Officers: A southwestern United States study

This study examined the legal training of Chief Housing Officers (CHOs) employed by colleges and universities in the southwest USA. The study also investigated the perceptions of CHOs with regard to legal issues that might confront their institutions in the next five years. The study further examined the possible differences in perceptions of CHOs who have held that position for less than five years versus individuals who have held the position for more than five years. Finally, the study makes recommendations for future research.     

10% or 5% match window in DNA profiling

Though the FBI repeatedly claims that the width of the window of the matching criterion they use in DNA profiling is 5%, it is shown that the width is indeed 10%. This fact has been noticed by some people but seems unknown to many. However, no one seems to be aware of the fundamental problem it creates to the fixed bin method employed by the FBI and some other laboratories because about half of the bins have sizes less than 10%. In other words, the probability for a random match of the DNA fragments from the crime scene and from the suspect could be underestimated. The problem may have serious implications for commonly adopted legal and forensic practices. The potential seriousness of underestimating the match probability is illustrated using the Hong Kong Chinese database.     

Rapid determination of scopolamine in evidence of recreational and predatory use

In recent years, scopolamine has become a drug of common use for recreational and predatory purposes and several ways of administration have been devised. A method for the rapid analysis of suspicious samples was developed, using a portable capillary electrophoresis with contactless conductivity detection. The method allows the separation of scopolamine from atropine which has a similar structure and is present along with scopolamine in some samples. The method was demonstrated to be useful for the fast analysis of several types of evidential items which have recently been reported to have been abused with fatal consequences or employed for criminal purposes. An infusion of Datura stramonium L., in which scopolamine and atropine naturally coexist, was analyzed for being frequently consumed for recreational purposes. A spiked moisturizing cream and six spiked alcoholic beverages were also analyzed. In spite of the complexity of the specimens, the sample pre-treatment methods developed were simple and fast.     

The limits and benefits of international mobility: an empirical examination of the biographies of Australian legal academics

ABSTRACT

Academics are becoming increasingly internationally mobile, and yet there is still limited research into the nature, outcomes and limits of academic mobility. This paper examines the biographies of over 700 academics employed within Australian law schools. It identifies legal academics who hold academics qualifications have been employed outside of Australia. Almost a quarter of legal academics in our sample hold a first degree outside of Australia, over a third hold a non-Australian post-graduate degree. While it could be expected that possessing international experience would broaden an academic’s cultural experiences, we also found that the internationally mobile academics have typically studied and worked previously within an elite international law school, and are now employed within an elite Australian law school. In addition, experiences of international mobility are not equally distributed, and male legal academics are significantly more likely to have international experience than female legal academics.     

Sensitive Method for the Confident Identification of Genetically Variant Peptides in Human Hair Keratin

Recent reports have demonstrated that genetically variant peptides derived from human hair shaft proteins can be used to differentiate individuals of different biogeographic origins. We report a method involving direct extraction of hair shaft proteins more sensitive than previously published methods regarding GVP detection. It involves one step for protein extraction and was found to provide reproducible results. A detailed proteomic analysis of this data is presented that led to the following four results: (i) A peptide spectral library was created and made available for download. It contains all identified peptides from this work, including GVPs that, when appropriately expanded with diverse hair-derived peptides, can provide a routine, reliable, and sensitive means of analyzing hair digests; (ii) an analysis of artifact peptides arising from side reactions is also made using a new method for finding unexpected modifications; (iii) detailed analysis of the gel-based method employed clearly shows the high degree of cross-linking or protein association involved in hair digestion, with major GVPs eluting over a wide range of high molecular weights while others apparently arise from distinct non-cross-linked proteins; and (v) finally, we show that some of the specific GVP identifications depend on the sample preparation method.     

单个细胞DNA样本的制备及其在灵敏度分析中的应用研究

目的建立一种用于高精确灵敏度分析的微量DNA样本制备方法。方法用显微捕获技术制备以细胞为单位的DNA样本,并用于ProfilerPlus试剂盒及ABI310遗传分析仪的灵敏度测试。结果建立了单细胞捕获操作方案,成功制备了依次含1～11个细胞的一组DNA分析样本,并可准确用于ProfilerPlus誖试剂盒及ABI310遗传分析仪的灵敏度研究。结论基于显微操作技术的高精确微量DNA分析样本制备方法可准确用于分析技术方法的灵敏度研究     

Anthropological facial ‘reconstruction’ – recognizing the fallacies, ‘unembracing’ the errors, and realizing method limits

Despite being practiced for over the last 200 years, facial approximation methods remain in their infancy as the soft tissue prediction methods employed have not been tested and justified. Scientific testing is the only way forward and much of it is needed. The lack of systematic scientific tests in the past has enabled many misleading notions to become established. Many of these notions appear to have arisen and been sustained as a result of practitioner biases--this is clearly evident even in the name commonly used to describe the method of building faces from skulls, for "facial reconstruction" implies everything the method is not, e.g., technical/scientific, exact, and credible. Although facial approximation methods are useful for forensic investigation (even if they do not generate identifications through true positive recognitions of the faces), the public should beware of the marketing and political ploys employed within the profession. These ploys give rise to some impressive, but unjustifiable claims--but do not just take my word for it; evaluate the evidence for yourself with disregard to the indoctrination waged by the facial "reconstruction" field in general, including that promoted by what I have had to say here. Use your own reason and intellect and see which conclusions you reach.     

目的解释方法及其意义

为克服严格法治的机械性 ,法学家提出了关于法律解释的目的方法。这种方法的确能在一定程度上缓解法律文本与法律价值的紧张关系。但是 ,法律的权威性及其意义的固定性则可能在解释中受到威胁 ,因而 ,为了达到既维护严格法治 ,又使法律呈现出灵活的价值选择 ,就必须有条件地应用目的解释方法     

The effects of skeletal preparation techniques on DNA from human and non-human bone

The forensic pathologist increasingly relies on the forensic anthropologist to be the consulting expert in human identification. Likewise, if identification is not possible from visual inspection of skeletal remains, the forensic biologist may be called upon to conduct DNA analysis. The possibility of downstream DNA testing needs to be considered when skeletal preparation techniques are employed to deflesh human remains, as they have the potential to strongly impact genetic analyses and subsequent identification. In this study, three cleaning techniques, boiling bone in water, in bleach, and in powdered detergent/sodium carbonate, were tested for their effect on nuclear and mtDNA recovery from a variety of human and non-human bones. A statistically significant reduction in DNA yields occurred in non-human bones cleaned with bleach, and DNA degradation was apparent electrophoretically. The human bones also showed much lower yields from bleach cleaning, while the detergent/carbonate method allowed the largest segments of DNA to be amplified, indicating it may have a less degradative effect on bone DNA than either of the other cleaning processes.     

染色毛干mtDNA不同提取方法的研究

目的为染色毛发线粒体DNA选择最佳的提取方法。方法在5个染发个体中各取5根头发,采用Chelex-100法、有机法、磁珠法、H2O2结合Chelex-100法及Chelex-100结合M icrocon100法等5种不同的提取方法提取染色毛发m tDNA,利用2%琼脂糖凝胶电泳检测扩增产物。结果Chelex-100结合M icrocon100方法提取染色毛发扩增m tDNA效果较好,其余方法无扩增产物。毛发的不同部位对扩增结果无影响。结论染色毛发采用恰当的提取方法可以提取到m tDNA模板,为法医日常检案提供帮助。     

CHANGES IN CRIMINAL OFFENDING AROUND THE TIME OF JOB ENTRY: A STUDY OF EMPLOYMENT AND DESISTANCE

Does employment promote desistance from crime? Most perspectives assume that individuals who become employed are less likely to offend than those who do not. The critical issue has to do with the timing of employment transitions in the criminal trajectory. The turning point hypothesis expects reductions in offending after job entries, whereas the maturation perspective assumes desistance to have occurred ahead of successful transitions to legitimate work. Focusing on a sample of recidivist males who became employed during 2001–2006 (N = 783), smoothing spline regression techniques were used to model changes in criminal offending around the point of entry to stable employment. Consistent with the maturation perspective, the results showed that most offenders had desisted prior to the employment transition and that becoming employed was not associated with further reductions in criminal behavior. Consistent with the turning point hypothesis, we identified a subset of offenders who became employed during an active phase of the criminal career and experienced substantial reductions in criminal offending thereafter. However, this trajectory describes less than 2 percent of the sample. The patterns observed in this research suggest that transition to employment is best viewed as a consequence rather than as a cause of criminal desistance.     

Typing of mitochondrial DNA from isolated cells of the human buccal epithelium

The authors have developed a method for molecular-genetic analysis of DNA from isolated cells for the purpose of forensic medical diagnostics. The method is based on the use of the laser capture microdissection (LCM) technology in combination with typing of mitochondrial DNA. Optimization of the conditions for amplification of polymorphic mtDNA loci in preparations containing minimal amounts of the genetic material was accomplished at the initial stage of the work. To this effect, the two-round polymerase chain reaction was employed that allowed the amplified material to be accumulated in the amount sufficient for sequenation. At the next stages, the system thus obtained was tested on the cell model (using isolated cells of human buccal epithelium). It was shown that the proposed method is suitable for the analysis of specific mtDNA characteristics in a single human cell.     

Estimation of stature from metacarpal lengths.

Formulae for the estimation of stature from metacarpal lengths are presented. Two samples of metacarpal specimens were employed in the analysis: one of 212 individuals from the Terry Collection, and one of 55 modern males, all of whom had measured statures. One measurement, the midline length, was taken on each metacarpal. Stature was regressed on the basis of the metacarpal length to derive equations for the Terry Collection individuals. Comparisons between the Terry Collection males and the modern sample showed the latter to have longer metacarpals and greater statures. The Terry equations were tested using the modern male sample. In spite of the differences noted, the Terry equations perform acceptably on modern individuals. The performance was slightly better for whites than for blacks. Since the female equations were not tested, they should be employed with greater caution.     

Determination of elemental sulfur in explosives and explosive residues by gas chromatography-mass spectrometry

A new method for the positive identification of elemental sulfur in explosives and explosive residues is developed. Following a carbon disulfide wash of explosives or explosive residues, a sample of the extracted material is injected onto a gas chromatography (GC) column, then analyzed via mass-selective (MS) detection. A positive identification of elemental sulfur is based on both retention time and fragmentation pattern. The GC-MS method is demonstrated to be useful in detecting and positively identifying elemental sulfur from both burned and unburned explosive mixtures. With a detection limit of 2.5 ng (2.5 x 10(-9) grams) of elemental sulfur on the column, it is shown to be 400 times more sensitive than the presumptive chemical color test that is currently the method employed for detection of small amounts of sulfur.     

Using hydrophilic adhesive tape for collection of evidence for forensic DNA analysis

Known exemplar samples of human DNA have traditionally been body fluids, such as blood, saliva, and semen. In each case, the presence of water is a risk for the bacterial growth, which may degrade the DNA evidence. In this study, the authors have developed a method that employed a hydrophilic adhesive tape (HAT) for collecting DNA evidence. The HAT method was used to remove surface cells from relatively hairless areas on the body. The area examined were ankle, arm, behind the ear, between fingers and back of the neck. The HAT was then dissolved in the extraction buffer. DNA typing was performed at vWA, THo1, F13A1, and FES loci using the short tandem repeat (STR) analysis. Our results show that the samples collected from ear give the best results with a success rate of 100%. All subjects tested by this method had known STR genotypes established from buccal swabs. The authors' results suggest that the HAT method can be used as a less invasive method for collecting biological evidence for forensic DNA analysis. In addition, this collection method should reduce the risk of DNA degradation due to the moisture, which is encountered using conventional collecting methods.     

Introduction of Therapy into Tegel Prison: Evaluation of an Experiment

The revision of the Penal Code of the Federal Republic of Germany includes provision for therapeutic units in prisons. This paper reports on the first of a series of research projects designed to test the effectiveness of the largest such unit which is at Tegel Prison in Berlin. The data indicate that residents of the unit have lower reconviction rates than prisoners exposed only to orthodox penal confinement. However, there are no significant differences in outcomes for the three treatment models employed. The methodological problems involved in this kind of evaluative research are discussed.