Sex Determination from the Second Cervical Vertebra: A Test of Wescott’s Method on a Modern American Sample*

Abstract: Numerous methods for establishing a biological profile exist; however, many of these methods rely on the recovery of several specific bones or on fragile skeletal elements that are sometimes irrecoverable. It is for this reason new methods utilizing other previously under‐documented bones should be established and tested by the forensic anthropological community. This study tests the accuracy of Wescott’s (J Forensic Sci 2000;45(2)) method for determining sex from the second cervical vertebra. Specimens were drawn from the donated skeletal collection curated at the Hamilton County Forensic Center (n = 57) and the William M. Bass Donated Skeletal Collection (n = 243). Both intra‐ and inter‐observer error rates were low and accurate classifications ranged from 78% (females‐Function 1) to 90.6% (males‐Function 5). Of the five functions, Function 4 achieved the highest overall accuracy, with 260 individuals (86.7%) falling into the correct category. Overall, this method is an effective classificatory tool for sex estimation.     

Sex variation in the second cervical vertebra

The second cervical vertebra can be used to estimate sex with 83% accuracy in unidentified human skeletal remains. Reported here are the necessary statistics, based on 8 dimensions taken from 400 second cervical vertebrae, for the computation of customized discriminant functions. Discriminant function equations developed using variables selected in a stepwise procedure are also presented here as an example of the usefulness of this bone in estimating sex.     

Sexual Dimorphism in the 7th Cervical and 12th Thoracic Vertebrae from a Mediterranean Population

Sex determination is an important task in physical anthropology and forensic medicine. The study sample comprised 121 individuals of known sex, age, and cause of death from San Jose cemetery in Granada (Spain). Eight dimensions were analyzed, and discriminant function analysis was performed for each vertebra to obtain discriminating functions and study the percentage of correct assignations of these functions. The percentage accuracy was approximately 80% for both vertebrae, but varied according to the sex, being higher for the 7th cervical in males and higher for the 12th thoracic in females. As reported in other populations, the greatest dimorphism values were found for the length of the inferior surface of the vertebral body and the width and length of the vertebral foramen of the 7th cervical vertebra and for the length of the inferior surface of the vertebral body of the 12th thoracic vertebra.     

Epiphyseal Union of the Cervical Vertebral Centra: Its Relationship to Skeletal Age and Maturation of Thoracic Vertebral Centra

Epiphyseal union stages for cervical vertebral centra (ring epiphyses) were documented for 55 individuals (females and males, ages 14–27 years) from the Terry Collection, using the Albert and Maples method 1, to examine both its relationship to age at death and to thoracic data collected from the same individuals using the same method. Results showed a moderate correlation between cervical ring union and age (r = 0.63, p = 0.000), and a fairly low correlation between cervical and thoracic ring union (r = 0.41, p = 0.002). Paired samples t‐tests yielded a statistically significant difference between cervical and thoracic union mean values (p = 0.01). Union progressed earlier in cervical vertebrae and in females. Results indicated fairly substantial variation in both sexes. Findings may serve as a basic guideline for estimating a general age range at death for unknown skeletal remains and to corroborate findings from other skeletal age indicators.     

Sex Assessment from the Sacral Base by Means of Image Processing

Abstract:  To help improve sex assessment from skeletal remains, the present study considers the diagnostic value of the sacral base (basis osseus sacri) based on its planar image and related metric data. For this purpose, 114 adult sacra of known sex and age from two early 20th century Italian populations were examined, the first from Bologna, northern Italy ( n  = 76), and the second from Sassari, Sardinia ( n  = 38). Digital photos of the sacral base were taken with each bone in a standardized orientation. Technical drawing software was used to trace its profile and to measure related dimensions (area, perimeter, and breadth of S1 and total breadth of the sacrum). The measurements were subjected to discriminant and classification function analyses. The sex prediction success of 93.2% for the Bolognese sample, 81.6% for the Sassarese sample, and 88.3% for the pooled sample indicates that the first sacral vertebra is a good character for sex determination.     

Testing Discriminant Functions for Sex Determination from Deciduous Teeth*,†

Abstract: Three studies have proposed discriminant functions for sex determination from deciduous tooth crown dimensions, and this study tests the existing functions on a sample of 46 Portuguese immature skeletons of known sex, aged from birth to 10 years. Deciduous teeth were measured in their mesiodistal and faciolingual crown dimensions, and percentage of correct allocation accuracy in determining sex using each specific function was determined. Discriminant functions were also calculated from data collected for this study and tested using cross‐validation. Results show poor overall accuracy (33.3–75%) and poor cross‐validation (46.2–60.0%). This is related to low sexual dimorphism in deciduous tooth crown size, as well as differences in degree of sexual dimorphism and in overall tooth size between different samples. For these reasons, deciduous crown size does not seem to show significant forensic value as discriminator of sex, particularly when methods developed on one population are applied to individuals of another population.     

A Reliable Regression‐Based Approach for Reassociating Human Skeletal Elements of the Lower Limbs from Commingled Assemblages

Accurate sorting of commingled human remains comprises a fundamental requirement for all further anthropological analyses. The lower limb bones are particularly important for reconstructing biological profiles. This study introduces a metric technique for sorting these elements using eight standard anthropological measurements and 222 adult individuals from Greece. The bones utilized were the os coxae, the femora, the tibiae and the tali. Simple regression analyses were used to develop functions for reassociating articulating bones, providing strong correlations (r = 0.74–0.95, p‐value <0.05) and high coefficients of determination (r²=0.54–0.91). Blind tests demonstrated that combining metric and morphoscopic techniques provides an excellent sorting accuracy for the hip and knee joints (ten of ten individuals), allowing for a reliable reassociation between a sex and age indicator (os coxae) and a body size indicator (femur). Overall, these results indicate the high value of metric methods in sorting commingled human remains.     

Discriminant function analysis for sex assessment in pelvic girdle bones: sample from the contemporary Mexican population

Sex assessment of skeletal remains plays an important role in forensic anthropology. The pelvic bones are the most studied part of the postcranial skeleton for the assessment of sex. It is evident that a population-specific approach improves rates of accuracy within the group. The present study proposes a discriminant function method for the sex assessment of skeletal remains from a contemporary Mexican population. A total of 146 adult human pelvic bones (61 females and 85 males) from the skeletal series pertaining to the National Autonomous University of Mexico were evaluated. Twenty-four direct metrical parameters of coxal and sacral bones were measured and subsequently, sides and sex differences were evaluated, applying a stepwise discriminant function analysis. Coxal and sacra functions achieved accuracies of 99% and 87%, respectively. These analyses follow a population-specific approach; nevertheless, we consider that our results are applicable to any other Hispanic samples for purposes of forensic human identification.     

Use of 2D:4D Digit Ratios to Determine Sex

Second to fourth digit ratios (2D:4D) are sexually dimorphic in human hands and established by the 13th gestational week. Application of 2D:4D for determining sex in living individuals by Kanchan et al. (Forensic Sci Int, 181, 2008, 53.e1) produced classification rates of 80% for males and 74–78% for females. Few studies have explored the use of 2D:4D for sexing skeletal remains. We test estimated finger lengths, phalanx lengths, and 2D:4D derived from hand bones for determining sex. Maximum phalanx length was collected using a mini‐osteometric board from 451 individuals of known age, sex, and ancestry in four skeletal collections. Logistic regression of 2nd and 4th digit finger and phalanx lengths produced classification rates greater than 80%. Digit ratios, however, failed to reach classification rates greater than 59%. Our results support those of Voracek (Forensic Sci Int, 185, 2009, e29) and suggest that 2D:4D may be population‐specific and thus inappropriate for universal application as a means of determining sex.     

Sexual Dimorphism of Anterior Sacral Curvature

Abstract: This study assesses the predictive value of anterior sacral curvature for sex estimation from skeletonized remains. Sacra from a sample of 125 American adults of known age and sex were examined. Nine measurements describing anterior sacral curvature were used in the analysis. Statistical treatment of the data included univariate statistics and discriminant function analysis for sex classification. A bootstrap validation method was employed to assess the classification error rates. Sacral curvature was significantly greater in men than in women at the level of the S2–S3 and S3–S4 articulations (p < 0.05). Correct classification estimates for the discriminant function range from 66–72%. Although sexually dimorphic, metric observations of sacral curvature are not as reliable at predicting sex as other skeletal elements. Anterior sacral curvature should only be used for sex estimation in the absence of other, more reliable, indicators.     

A Method for Estimating Sex Using Metric Analysis of the Scapula*

Abstract: The most accurate and precise methods for the assessment of age and stature often require knowledge of sex. Thus, being able to correctly identify sex from skeletal remains is critical in the forensic context. The presence of the os coxae or skull can never be guaranteed, making the development of reliable methods of sex estimation using other skeletal elements necessary. Using a 724 individual calibration sample from the Hamann‐Todd collection, this study identifies sexual dimorphism in the human scapula, and presents a new five‐variable discriminant function for sex estimation. The overall accuracy of this method proved to be 95.7% on the cross‐validated calibration sample, 92.5% on an 80 individual test sample from the Hamann‐Todd collection, and 84.4% on a 32 individual test sample from the skeletal collection of the Wichita State University Biological Anthropology Laboratory. Additionally, a slightly less accurate two‐variable model was developed and has cross‐validated accuracy of 91.3%.     

Mastoid Triangle for Sex Determination in Adult Nigerian Population: A Validation Study

In the quest for a simple, reliable technique to estimate the sex of human remains several novel metric skeletal indices have been reported. Only a few have been examined for utility in populations different from those in which they were developed. In this study, the mastoid process was evaluated for sex determination using 102 lateral cephalograms of a Nigerian sample of known age and sex. The asterion‐mastoidale distance and mastoid triangular area were sexually dimorphic with mean values higher in males compared with females (p = 0.02). On analysis of the discriminant function, overall accuracy for sex classification was 55%. On cross‐validation, the triangular area accurately identified 80% of females and 48% of males. The asterion‐mastoidale distance was slightly more accurate at sexing the sample. The practical utility of the mastoid triangle area technique to differentiate sex in Nigerian populations is not supported by the results of this study.     

Sexual Dimorphism of the Arm Bones in a Modern Greek Population

Abstract: Several studies have shown that sex determination methods based on measurements of the skeleton are population specific. Metric traits of the long bones of the arm have been reported as reliable indicators of sex. This study was designed to determine whether the three long bones of the arm can be used for sex determination on a skeletal population from Greece. The material used consists of the arm bones of 204 adult individuals (111 males and 93 females) coming from the Modern Human Skeletal Collection of the University of Athens. The age range is 19–96 years for males and 20–99 years for females. The maximum lengths and epiphyseal widths were measured in the long bones of the arm (humerus, radius, and ulna). The discriminant analysis of the metrical data of each long bone gave very high discrimination accuracies. The rate of correct sex discrimination based on different long bones ranges from 90.30% (ulna) to 95.70% (humerus). In addition, intra‐ and inter‐observer error tests were performed. These indicated that replication of measurements was satisfactory for the same observer over time and between observers. The results of this study show that metric characteristics of the arm bones can be used for the determination of sex in skeletal remains from Greece and that bone dimensions are population specific.     

Reliability of the Walker Cranial Nonmetric Method and Implications for Sex Estimation

The cranial trait scoring method presented in Buikstra and Ubelaker (Standards for data collection from human skeletal remains. Fayetteville, AR: Arkansas Archeological Survey Research Series No. 44, 1994) and Walker (Am J Phys Anthropol, 136, 2008 and 39) is the most common nonmetric cranial sex estimation method utilized by physical and forensic anthropologists. As such, the reliability and accuracy of the method is vital to ensure its validity in forensic applications. In this study, inter‐ and intra‐observer error rates for the Walker scoring method were calculated using a sample of U.S. White and Black individuals (n = 135). Cohen's weighted kappas, intraclass correlation coefficients, and percentage agreements indicate good agreement between trials and observers for all traits except the mental eminence. Slight disagreement in scoring, however, was found to impact sex classifications, leading to lower accuracy rates than those published by Walker. Furthermore, experience does appear to impact trait scoring and sex classification. The use of revised population‐specific equations that avoid the mental eminence is highly recommended to minimize the potential for misclassifications.     

Sex assessment from carpals bones: discriminant function analysis in a contemporary Mexican sample

Sex assessment is one of the first essential steps in human identification, in both medico-legal cases and bio-archaeological contexts. Fragmentary human remains compromised by different types of burial or physical insults may frustrate the use of the traditional sex estimation methods, such as the analysis of the skull and pelvis. Currently, the application of discriminant functions to sex unidentified skeletal remains is steadily increasing. However, several studies have demonstrated that, due to variation in size and patterns of sexual dimorphism, discriminant functions are population-specific. In this study, in order to improve sex assessment from skeletal remains and to establish population-specific discriminant functions, the diagnostic values of the carpal bones were considered. A sample of 136 individuals (78 males, 58 females) of known sex and age was analyzed. They belong to a contemporary identified collection from the Laboratory of Physical Anthropology, Faculty of Medicine, UNAM (Universidad Nacional Autónoma de México, Mexico City). The age of the individuals ranged between 25 and 85 years. Between four and nine measurements of each carpal bone were taken. Independent t-tests confirm that all carpals are sexually dimorphic. Univariate measurements produce accuracy levels that range from 61.8% to 90.8%. Classification accuracies ranged between 81.3% and 92.3% in the multivariate stepwise discriminant analysis. In addition, intra- and inter-observer error tests were performed. These indicated that replication of measurements was satisfactory for the same observer over time and between observers. These results suggest that carpal bones can be used for assessing sex in both forensic and bio-archaeological identification procedures and that bone dimensions are population specific.     

Determination of sex for the 12th thoracic vertebra by morphometry of three-dimensional reconstructed vertebral models

Thirty-three linear measurements and two ratios were derived from 102 12th thoracic vertebrae of the Digital Korean database at the Catholic Institute for Applied Anatomy. Of 35 linear traits, 23 were sexually dimorphic. We created 23 discriminant function equations that predicted sex with 62.7-85.3% accuracy. The analysis using combinations of two factors gave higher accuracies: most equations with accuracies over 80% included at least one measurement involving the coronal diameter of the vertebral endplate. Using stepwise method of discriminant function analysis, three variables predicted sex with 90.0% accuracy: the coronal diameter of the superior endplate of the vertebral body, the ratio of anterior to middle height of the body, and the length of the left mammillary process and pedicle. Coronal dimensions of the vertebral body represented the major sex difference. These equations will help forensic discrimination of the sex of this vertebra among Koreans.     

Quantification of Pubic Symphysis Metamorphosis Based on the Analysis of Clinical MDCT Scans in a Contemporary Malaysian Population

Forensic age estimation methods are biased to sex and population; in general, accuracy is reduced when applied to foreign populations. This study assessed the accuracy of the Suchey–Brooks method in contemporary Malaysian individuals and aimed to formulate population‐specific standards. Multi‐detector computed tomography scans of 355 individuals (165 male; 190 female) of 15–83 years of age were reconstructed using 3D‐volumetric rendering in RadiAnt. Pubic symphyseal phase, bias, inaccuracy, and percentage correct age classifications are examined. Transition analysis was used to develop age estimation standards. High observer agreement (κ = 0.763–0.832) and a positive relationship between age and pubic symphyseal phase (r = 0.884–0.90) were demonstrated. Mean inaccuracies were 8.62 and 8.95 years for males and females, respectively; overall correct classification was 97.8%. Transition ages between phases in males were 18.79, 23.29, 28.85, 43.64, and 61.15 years; in females, the corresponding data were 19.77, 22.53, 32.62, 41.85, and 57.39 years.     

Sexual Dimorphism of Maxillary Sinus Dimensions Using Cone‐Beam Computed Tomography

Sex determination of unknown persons plays an important role in forensic medicine. Cone‐beam computed tomography (CBCT) is an excellent imaging modality for accurate measurement of maxillary sinus dimensions. This study aimed to evaluate whether maxillary sinus measurements on CBCT scans can be applied for sex determination. The width, length, and height of maxillary sinuses in CBCT images of 100 patients (50 males and 50 females) were measured. Student's t‐test and discriminant function analysis were used to compare differences in the measured parameters between males and females. The correct predictive accuracy rate of sex determination was 78% in females and 74% in males with overall accuracy of 76%. Based on discriminant analysis, the most pronounced variable in differentiation of sex groups was maxillary sinus height. This study suggests that maxillary sinus measurements can be valuable for sex determination in forensic investigation, especially for cases in which other traditional methods are not applicable.     

Sex Determination by Discriminant Function Analysis of Lumbar Vertebrae

Sex determination is critical for developing the biological profile of unidentified skeletal remains. When more commonly used elements (os coxa, cranium) for sexing are not available, methods utilizing other skeletal elements are needed. This study aims to assess the degree of sexual dimorphism of the lumbar vertebrae and develop discriminant functions for sex determination from them, using a sample of South African blacks from the Raymond A. Dart Collection (47 males, 51 females). Eleven variables at each lumbar level were subjected to univariate and multivariate discriminant function analyses. Univariate equations produced classification rates ranging from 57.7% to 83.5%, with the highest accuracies associated with dimensions of the vertebral body. Multivariate stepwise analysis generated classification rates ranging from 75.9% to 88.7%. These results are comparable to other methods for sexing the skeleton and indicate that measures of the lumbar vertebrae can be used as an effective tool for sex determination.