共查询到18条相似文献,搜索用时 218 毫秒
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利用DNA重组技术,由pUC19-MYO质粒中获得MYO小卫星DNA探针片段,将其插入到具有RNA聚合酶启动子的pGEM-4Z质粒的多克隆位点,从而构建了pGEM-4Z一MYO亚克隆,为制备RNA探针并将其应用于DNA指纹检测,提高DNA指纹的敏感性和小卫星DNA多态性的检出率奠定了基础。 相似文献
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C.Rittner U.Schacker G.Rittner T.Breidbach B.Holtkamp H.H.Sonneborn P.M.Schileider 陈跃龙 《中国法医学杂志》1988,(3)
<正> 小卫星DNA探针MZ1.3是从人类基因库中通过筛选随机选择克隆中的超可变重复序列而分离的。详细制备方法及该选择性DNA克隆序列的细节将另行发表。自外周血细胞及尸解材料组织标本抽提的人DNA用限制性内切酶HinfI酶解,再作Southern印迹法分析。在与经~(32)P-dCTP缺口翻译的探针MZ1.3杂交后,经放射性自显影,每一个体DNA样本平 相似文献
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<正> 在人类DNA上发现了许多事变区,它们通常是由前后重复被称为小卫星的短顺列所组成。由于高度变异性,含有微小卫星的DNA为个体识别及亲子鉴定提供了有价值的遗传探针。为了分离出更多的有效的DNA探针,我们建立了一个由许多高度DNA片段组成的Cha- 相似文献
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单位点DNA探针在早孕期亲子鉴定中的应用研究 总被引:2,自引:0,他引:2
本研究利用限制性片段长度多态性分析技术,从3O例早孕期绒毛组织及其父母血液中提取DNA,以两种单位点DNA探针PAC255及PAC256进行检测,根据所获等位基因的大小,查国人此二位点基因频率及每一家系三组合中该基因传递相对机会,计算出相应的亲子关系指数和亲子关系概率。结果.累积条子关系指数和累积亲子关系概率的范围分别是56.80~8333.50和98.27~99.99%、按照国际通用标准,这30个家系中,可以肯定亲手关系者20例(66.7%),极可能有亲子关系者8例(26.7%),非常可能有亲子关系者2例(6.7)。说明同时使用此二单位点探针,在早孕期亲子鉴定中有很高的应用价值。 相似文献
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使用6个单位点探针,用Southern印迹杂交技术研究了国人的D2S44,D17S79,D14S13,D14S1,DXYS14及D18S27等位点的基因频率分布。发现它们的DNA指纹图均具高度多态性,等位基因数27~107个。非父排除率(EPP)分别为0.8407、0.7691、0.9792、0.9040、0.9400及0.8177,累积非父排除率为0.99999;个人识别能力(DP)分别为0.9880、0.9766,0.9996,0.9961,0.9973及0.9338,累积个人识别能力接近1,故可作为亲子鉴定及个人识别非常有力的工具。此外还对单位点探针的优缺点进行了讨论。 相似文献
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B Olaisen B Mev?g R Jonassen G Paulsen E Thorsby P Teisberg 《Zeitschrift für Rechtsmedizin》1987,99(1):55-64
A restriction fragment length polymorphism analysis using double digestion of DNA preparations with XbaI and BglII restriction enzymes and hybridization with C4 and HLA-DR probes is described. The typing conditions selected reveal extensive individual variation in both C4 and DR gene regions. In our panel of 46 unrelated individuals, 37 different phenotypic patterns were recognized when both probes were used, and preliminary discriminative power values of 0.865 and 0.914 were calculated for C4 and DR beta, respectively. The probability of a chance match using both systems is probably about 1.5.10(-2). The potential of this method for individual identification of blood stains was demonstrated on DNA prepared from 6-month-old dried blood stains from seven panel individuals. The seven individuals were all identified when comparing stain DNA patterns with panel control patterns. No RFLP pattern changes were observed following storage of blood stains. Based on these experiments with C4 and DR beta DNA typing under laboratory conditions, it is concluded that DNA typing with such probes may become a powerful tool in future stain identification analyses. 相似文献
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应用 MYO DNA 探针对中国人进行了 DNA 遗传指纹图检验。从两个家系16人及100个无关个体中取肘静脉血提取 DNA,用限制性内切酶 HinfⅠ或 HaeⅢ水解,1%琼脂糖凝胶电泳分离,经 Southern印迹转移,MYO DNA 探针杂交,获得了清晰可辨的 DNA 指纹图谱。结果每个个体在3.0Kb 以上均能检出10条以上杂交区带,个体间的相关概率<4×10~(-9),由杂交区带构成的图谱是个体特异的,杂交区带遵循孟德尔的显性遗传方式由亲代向子代遗传;具有 DNA Fingerprints 的特点。对两起亲子鉴定的案例进行指纹图检验,孩子所存在的杂交区带,除来自母亲外,其余可在嫌疑父亲带中找到,肯定了孩子与嫌疑人的父子关系。MYO DNA 探针在亲子鉴定与个人识别的法医学鉴定中有着重要的实用价值。 相似文献
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Species identification is important in many big game forensic science cases but cannot always be accomplished because of the lack of adequate techniques. The authors have developed deoxyribonucleic acid (DNA) probes for elk, deer, and antelope by isolating highly repeated satellite sequences. These DNA probes distinguish among deer, elk, and antelope, although not between different species of deer. Because of the high number of sequence copies per genome, these probes are extremely sensitive, requiring less than 10 ng of total genomic DNA. The developmental protocol for these probes is relatively simple and is applicable to many other species. 相似文献