The determination of phoxim in cadaveric material by gas-liquid chromatography]

The developed gas-liquid chromatographic procedure for forensic chemical detection and measurement of phoxim in cadaveric material permits the detection in 25 g of an organ of 44 to 52% of added phoxim. The threshold of phoxim measurement in 100 g of the liver is 0.04 mg, the least volume of phoxim detectable being 0.02 g. The developed gas-liquid chromatographic technique should be used as an additional method together with thin-layer chromatography, for a more objective evaluation of the results of forensic chemical analysis of cadaveric material for phoxim.     

The gas chromatographic determination of carbophos in cadaveric material

The authors developed a method of malathion insecticide determination in the cadaveric material (blood and wall of the stomach) including extraction of 5 ml of blood (5 g of fragmented tissue of stomach wall) by hexan; preparative chromatographic purification of extract on silicagel layer L 5/40 mkm in hexan-diethyl ether system 10:2; Gas-liquid chromatographic investigation on column with 5% SE-30 and 5% XE-60 (Chromatograph "Colour-106" with thermionic detector). This method makes it possible to detect 72-76% of malathion added.     

A method for determining molybdenum in cadaveric material by using alkylphenylcyclohexylcarboxylic acid]

Alkylphenylcyclohexylcarboxylic acid was found to be an efficient extractant to extract molybdenum from the cadaveric material. Method suggested is simple enough for use and doesn't require considerable amounts of chemical reagents.     

The detection of novocaine and p-aminobenzoic acid in cadaveric material

Microcrystalloscopic reactions, TLC and UV spectroscopy methods for novocainum and p-aminobenzoic acid identification in medicolegal examination of the cadaveric material were developed. Sensitivity and specificity of microcrystalloscopic reactions were studied.     

The isolation of amitriptyline from cadaveric material using acetonitrile

Method of amitriptyline isolation from the cadaveric material using acetonitrile as extractant is suggested. This method makes it possible to extract in average 76.09% of substance previously added in quantity of 1 mg to 25 g of the liver. Method was successfully used in analysis of expert material.     

ELISA-based determination of opiates in purulent transformation of cadaveric material

Abuse of drugs has been a topical issue up to the present-day. Opiates like morphine and heroin are still prevalent. The routine methods of determination of drugs in blood made within the forensic medical expertise are time-consuming and samples must be specially prepared for them. ELISA, when used for the determination of opiates in blood and urine, significantly cuts the test time. According to our research results, the diagnosis of opiates by ELISA is advisable at the preliminary stage of the forensic expertise of cadaver; the method is informative, which is of extra importance in cases of purulent transformation of cadaveric material.     

The analysis of tiapride in cadaveric material

To optimize conditions of tiapride isolation from cadaveric organs, we compared the results of conventional methods by Stas-Otto, A.A. Vasilyeva and V.F. Kramarenko which provide tiapride isolation up to 50% and a new precise and reproducible method providing 60 +/- 2% tiapride isolation. Identification of tiapride isolated from cadaveric material was made with thin-layer chromatography and high performance liquid chromatography. The latter assay employed the method of external standard. The original techniques proposed identify and measure tiapride in hepatic samples in the presence of unidentified endogenic compounds. The techniques are rapid, selective, sensitive and reproducible.     

The retention of alkyldinitrophenol compounds in cadaveric material]

The preservation of alkyl dinitrophenol compounds in corpse liver tissue was under study. The content of these compounds was assessed after various periods of the corpse storage.     

The detection and separation of antiparkinson preparations in cadaveric material by gas-liquid chromatography

Method of gas-chromatographic detection of anti-parkinsonism substances (amedine, amysile, dinesine, midantane, tropazine and cyclodole) isolated from the cadaveric material is suggested. Conditions for separation of anti-parkinsonism substances when they are all present in the cadaveric material are developed.     

Preservation of methanol in cadaveric material

Duration of preservation of methanol in cadaveric material at 20 +/- 3 degrees C during 4-13 months was studied on 19 model and expert objects of cadaveric material (muscle tissue, kidney, blood, urine). Exponential relationship between methanol concentration and duration of storage of the object was detected. Methanol concentration decreased by 30-70% during the first weeks, after which the process decelerated to 5-10% a month. Toxicologically significant concentrations of endogenous methanol were not revealed in any case.     

Determination of trasodone in cadaveric material

Methods of trasodone isolation from cadaveric material by water and acetonitrile at pH-2.0, which may be used in expert practice, were developed. Acetonitrile method is recommended for everyday practice as it allows one to isolate about 60% of trasodone and to obtain extracts with lower content of extractive substances. Detection limit in case of trasodone isolation by acidified water is 50 micrograms in 25 g of the liver and by acetonitrile--25 micrograms in 25 g of the liver and kidney.