An Investigation into the Relationship between Human Cranial and Pelvic Sexual Dimorphism

When faced with commingled remains, it might be assumed that a more “masculine” pelvis is associated with a more “masculine” cranium, but this relationship has not been specifically tested. This study uses geometric morphometric analyses of pelvic and cranial landmarks to assess whether there is an intra‐individual relationship between the degrees of sexual expression in these two skeletal regions. Principal component and discriminant function scores were used to assess sexual dimorphism in 113 U.S. Black individuals. Correlation values and partial least squares regression (PLS) were used to evaluate intra‐individual relationships. Results indicate that the os coxae is more sexually dimorphic than the cranium, with element shape being more sexually dimorphic than size. PLS and correlation results suggest no significant intra‐individual relationship between pelvic and cranial sexual size or shape expression. Thus, in commingled situations, associations between these skeletal elements cannot be inferred based on degree of “masculinity.”     

Sex Estimation from Human Cranium: Forensic and Anthropological Interest of Maxillary Sinus Volumes

Sex estimation is a key objective of forensic science. We aimed to establish whether maxillary sinus volumes (MSV) could assist in estimating an individual's sex. One hundred and three CT scans were included. MSV were determined using three‐dimensional reconstructions. Two observers performed three‐dimensional MSV reconstructions using the same methods. Intra‐ and interobserver reproducibility were statistically compared using the intraclass correlation coefficient (ICC) (α = 5%). Both intra‐ and interobserver reproducibility were perfect regarding MSV; both ICCs were 100%. There were no significant differences between right and left MSV (p = 0.083). No correlation was found between age and MSV (p > 0.05). We demonstrated the existence of sexual dimorphism in MSV (p < 0.001) and showed that MSV measurements gave a 68% rate of correct allocations to sex group. MSV measurements could be useful to support sex estimation in forensic medicine.     

Sexual Dimorphism in Auricular Surface Projection and Postauricular Sulcus Morphology

The presence of an elevated auricular surface and a postauricular sulcus is presented in numerous reference books as osteological indicators of sex, but the validity of these traits has not been adequately evaluated. The ilia of 322 (181 male, 141 female) adults were examined, the auricular surface was scored as completely, partially, or nonelevated, and the postauricular sulcus was scored as present or absent. Complete elevation occurred almost exclusively in females (67% of females and 0.6% of males). The postauricular sulcus was present in 27% of males and 85% of females. When present, an elevated auricular surface is a reliable indicator that the individual is female. However, the absence of the trait is a less reliable indicator of sex. The postauricular sulcus is a moderately accurate estimator of sex.     

Sexual dimorphism in America: geometric morphometric analysis of the craniofacial region

One of the four pillars of the anthropological protocol is the estimation of sex. The protocol generally consists of linear metric analysis or visually assessing individual skeletal traits on the skull and pelvis based on an ordinal scale of 1-5, ranging from very masculine to very feminine. The morphologic traits are then some how averaged by the investigator to estimate sex. Some skulls may be misclassified because of apparent morphologic features that appear more or less robust due to size differences among individuals. The question of misclassification may be further exemplified in light of comparisons across populations that may differ not only in cranial robusticity but also in stature and general physique. The purpose of this study is to further examine the effect of size and sex on craniofacial shape among American populations to better understand the allometric foundation of skeletal traits currently used for sex estimation. Three-dimensional coordinates of 16 standard craniofacial landmarks were collected using a Microscribe-3DX digitizer. Data were collected for 118 American White and Black males and females from the W.M. Bass Donated Collection and the Forensic Data Bank. The MANCOVA procedure tested shape differences as a function of sex and size. Sex had a significant influence on shape for both American Whites (F = 2.90; d.f. = 19, 39; p > F = 0.0024) and Blacks (F = 2.81; d.f. = 19, 37; p > F = 0.0035), whereas size did not have a significant influence on shape in either Whites (F = 1.69; d.f. = 19, 39; p > F = 0.08) or Blacks (F = 1.09; d.f. = 19, 37; p > F = 0.40). Therefore, for each sex, individuals of various sizes were statistically the same shape. In other words, while significant differences were present between the size of males and females (males on average were larger), there was no size effect beyond that accounted for by sex differences in size. Moreover, the consistency between American groups is interesting as it suggests that population differences in sexual dimorphism may result more from human variation in size than allometric variation in craniofacial morphology.     

Sexual Dimorphism in Finger Ridge Breadth Measurements: A Tool for Sex Estimation from Fingerprints

Previous research has demonstrated significant sexual dimorphism in friction ridge skin characteristics. This study uses a novel method for measuring sexual dimorphism in finger ridge breadths to evaluate its utility as a sex estimation method from an unknown fingerprint. Beginning and ending in a valley, the width of ten parallel ridges with no obstructions or minutia was measured in a sample of 250 males and females (N = 500). The results demonstrate statistically significant differences in ridge breadth between males and females (p < 0.001), with classification accuracy for each digit varying from 83.2% to 89.3%. Classification accuracy for the pooled finger samples was 83.9% for the right hand and 86.2% for the left hand, which is applicable for cases where the digit number cannot be determined. Weight, stature, and to a lesser degree body mass index also significantly correlate with ridge breadth and account for the degree of overlap between males and females.     

Ontogeny of Size and Shape Sexual Dimorphism in the Pubis: A Multislice Computed Tomography Study by Geometric Morphometry

Few studies have been conducted to determine sex differences in the immature coxal bone and the results were often contradictory. The authors studied sexual dimorphic differences of the pubis using geometric morphometric analysis of five osteometric landmarks recorded by multislice computed tomography (MSCT), based on three‐dimensional reconstructions of 188 children (95 boys, 93 girls) living in the region of Toulouse, southwestern France, ranging in age from 1 to 18 years old. They used geometric morphometric methodology first to test sexual dimorphism in size (centroid size) and shape (Procrustes residuals) and second to examine patterns of shape change with age (development) and size change with age (growth). Based on statistical significance test results, the pubic shape became sexually dimorphic at 13 years old, although visible shape differences were observed as early as 9 years old. This work showed that the trajectories of pubis shape (development) and size (growth) differed throughout ontogeny and between sexes.     

Sexual Dimorphism of the Fetal Ilium: A 3D Geometric Morphometric Approach with Multislice Computed Tomography

Adult sexual dimorphism is well established. However, data regarding fetuses are sparse with contradictory results. Our study aimed to look for fetal sexual dimorphism. Multislice computed tomography (MSCT) examinations and three‐dimensional (3D) reconstructions were recorded on 93 fetal iliac bones aged between 21 and 40 weeks of amenorrhea (WA). The collection was divided into two age groups, with a cutoff at 30 WA. The 3D geometric morphometric analysis was based on outline analysis. We used elliptic Fourier analysis (EFA) because of the difficulty of landmark positioning. Step‐by‐step reconstructions allowed a better comprehension of the fine shape details. Principal component analysis (PCA) and linear discriminant analysis (LDA) were performed on the two age groups. Results did not show any difference between male and female shapes, in both age groups. We demonstrate the feasibility of outline analysis for immature shapes, but sexual dimorphism has not been established in our collection.     

Ontogeny of Size and Shape Sexual Dimorphism in the Ilium: A Multislice Computed Tomography Study by Geometric Morphometry

We studied sexually dimorphic differences in the ilium using geometric morphometric analysis of 10 osteometric landmarks recorded by multislice computed tomography, based on three‐dimensional reconstructions of 188 children (95 boys, 93 girls) of mixed origins living in the area of Toulouse, southern France, and ranging in age from 1 to 18 years. We used geometric morphometrics methodology first to test sexual dimorphism in size (centroid size) and shape (Procrustes residuals) and second to examine patterns of shape change with age (development) and size change with age (growth). On the basis of statistical significance testing, the ilium shape became sexually dimorphic at 11 years of age, although visible shape differences were observed as early as 1 year of age. There was no statistically significant difference in size between sexes. Trajectories of shape (development) and size (growth) differed throughout ontogeny and between sexes.     

Improving Sex Estimation from Crania Using a Novel Three‐dimensional Quantitative Method,,

This study utilizes an innovative 3D approach to discover metric variables that obtain the highest classification rates for sex estimation from the cranium. Models were constructed from 222 cranial CT scans of U.S. Whites from the Bass Donated Collection. These models were used to create a statistical bone atlas that captures the primary shape variation in the skull and facilitates rapid computer‐automated analyses. The bone atlas showed that important size‐related sex variables are bizygomatic breadth, maximum cranial length, cranial base length, and mastoid height. Shape‐related variables capture sex differences in the projection of the glabellar region, inclination of the frontal, and cranial base flexion. In addition, vault thickness is highly dimorphic, with females having on average thicker vaults in the frontal region, and males having thicker vaults in the occipital region. Cross‐validated linear discriminant analysis obtained >95% accuracy (97.5% with 11 variables and 95.5% with eight variables).     

Sexual Dimorphism of Anterior Sacral Curvature

Abstract: This study assesses the predictive value of anterior sacral curvature for sex estimation from skeletonized remains. Sacra from a sample of 125 American adults of known age and sex were examined. Nine measurements describing anterior sacral curvature were used in the analysis. Statistical treatment of the data included univariate statistics and discriminant function analysis for sex classification. A bootstrap validation method was employed to assess the classification error rates. Sacral curvature was significantly greater in men than in women at the level of the S2–S3 and S3–S4 articulations (p < 0.05). Correct classification estimates for the discriminant function range from 66–72%. Although sexually dimorphic, metric observations of sacral curvature are not as reliable at predicting sex as other skeletal elements. Anterior sacral curvature should only be used for sex estimation in the absence of other, more reliable, indicators.     

A Validation Study of the Langley et al. (2017) Decision Tree Model for Sex Estimation

Langley et al. (2017) developed a sex estimation decision tree utilizing two traditional cranial traits (glabella and mastoid) and a new trait: zygomatic extension. This study aimed to test the reliability of their zygomatic extension scoring method and validate their sex estimation method. Ordinal score data were collected from 281 male and female U.S. White and Black individuals. The five traditional cranial traits were collected from physical specimens, while zygomatic extension was scored from 3D cranial models. Intra‐ and interobserver analyses carried out on a subsample of 30 individuals indicate good agreement between zygomatic scores. The decision tree correctly sexed 71.5% of the sample, but a strong sex bias (94.2% correct for females, 49.3% correct for males) severely limits the utility of this method. The Walker (2008) and Stevenson et al. (2009) methods produced higher accuracy rates (80.8% and 82.6%, respectively), although these methods also produced sex and ancestry biases.     

Sexual Dimorphism of the Humerus in Contemporary Cretans—A Population‐Specific Study and a Review of the Literature*

Abstract: Sex determination is the first essential step for positive identification when a decomposed body is recovered. Taking into consideration the population aspect of sexual dimorphism of the skeleton, the present study aimed to create a sex identification technique using osteometric standards, derived from a contemporary Cretan population. A total of 168 left humeri were measured according to standard osteometric techniques. The differences between the means in males and females were significant (p < 0.0005). About 92.3% of cases were correctly classified when all measurements were applied jointly. Stepwise procedure produced an accuracy rate of 92.9%. The most effective single dimension was vertical head diameter (89.9%). The current study provides standards for a population that has not been represented so far in the existing databases. It demonstrates that the humerus is an effective bone for the estimation of sex because even in a fragmentary state it can give high classification accuracy.     

The Influence of Body Size on the Expression of Sexually Dimorphic Morphological Traits

Skeletal sexual dimorphism manifests as size or shape differences between males and females in a population. Certain dimorphic traits are used in sex estimation methods, and populational variation in the expression of these traits can result in inaccurate sex estimation. However, the underlying causes of variation in trait expression remain unclear. This study explores body size, which also exhibits sexual dimorphism, as a potential factor influencing trait expression. To test this, skeletons of 209 individuals of varying body size were analyzed, and morphological traits were scored according to the Walker (2008), Klales et al. (2012), and Rogers (1999) sex estimation methods. Statistical analyses found significant correlations between body size parameters and expression of traits, with stature explaining more relative variance in trait expression than body mass. However, the relationships are weak and few in number, suggesting that body size has a minimal impact on the expression of these morphological traits.     

Sexual Dimorphism in Ulna: An Osteometric Study from India

Determination of sex constitutes the most important element during the identification process of human skeletal remains. Several sex‐specific features of human skeleton have been exploited for sex determination with varying reliability. This study aims to obtain sexual dimorphic standards for ulnae of the north Indian population. Eight measurements were obtained on a sample of 106 ulnae (males‐80, females‐26) in the age range of 25–65 years. The sexual dimorphism index and demarking points were calculated for all the variables. The data were then subjected to stepwise and direct discriminant function analysis. The best discriminator of sex was the maximum length (84.9%) followed by radial notch width (84%). In stepwise analysis, these two variables were selected and provided an accuracy of 88.7% (M‐87.5%, F‐92.3%). The proximal end provided a classification rate of 81.1% (M‐80%, F‐84.6%) with selection of the notch length and olecranon width.     

Odontological Approach to Sexual Dimorphism in Southeastern France

Abstract: The aim of this study was to establish a prediction formula to allow for the determination of sex among the southeastern French population using dental measurements. The sample consisted of 105 individuals (57 males and 48 females, aged between 18 and 25 years). Dental measurements were calculated using Euclidean distances, in three‐dimensional space, from point coordinates obtained by a Microscribe. A multiple logistic regression analysis was performed to establish the prediction formula. Among 12 selected dental distances, a stepwise logistic regression analysis highlighted the two most significant discriminate predictors of sex: one located at the mandible and the other at the maxilla. A cutpoint was proposed to prediction of true sex. The prediction formula was then tested on a validation sample (20 males and 34 females, aged between 18 and 62 years and with a history of orthodontics or restorative care) to evaluate the accuracy of the method.     

Sexual Dimorphism of the First Rib: A Comparative Approach Using Metric and Geometric Morphometric Analyses

This research investigated the sexual dimorphism of the first human rib using geometric morphometric and metric approaches on a sample of 285 specimens containing European Americans and African Americans from the Hamann‐Todd collection. Metric measurements were investigated for sexual dimorphism and ancestral differences using univariate statistics. Four type II landmarks and 40 sliding semi‐landmarks were placed outlining the dorsal and ventral curvatures of the ribs. Landmark data were processed using Generalized Procrustes Analyses with Procrustes distance sliding, and the subsequent coordinates were investigated for sexual dimorphism and ancestral differences using Procrustes ANOVAs. Both geometric morphometric and metric data were analyzed using cross‐validated discriminant function analyses to test the hypothesis that variables from both approaches can be combined to increase sex classification rate. European Americans had sex correctly classified as high as 88.05% and African Americans as high as 70.86% using a combination of metric and geometric morphometric variables.     

A Method for Estimating Sex Using Metric Analysis of the Scapula*

Abstract: The most accurate and precise methods for the assessment of age and stature often require knowledge of sex. Thus, being able to correctly identify sex from skeletal remains is critical in the forensic context. The presence of the os coxae or skull can never be guaranteed, making the development of reliable methods of sex estimation using other skeletal elements necessary. Using a 724 individual calibration sample from the Hamann‐Todd collection, this study identifies sexual dimorphism in the human scapula, and presents a new five‐variable discriminant function for sex estimation. The overall accuracy of this method proved to be 95.7% on the cross‐validated calibration sample, 92.5% on an 80 individual test sample from the Hamann‐Todd collection, and 84.4% on a 32 individual test sample from the skeletal collection of the Wichita State University Biological Anthropology Laboratory. Additionally, a slightly less accurate two‐variable model was developed and has cross‐validated accuracy of 91.3%.     

Sexual Dimorphism in the 7th Cervical and 12th Thoracic Vertebrae from a Mediterranean Population

Sex determination is an important task in physical anthropology and forensic medicine. The study sample comprised 121 individuals of known sex, age, and cause of death from San Jose cemetery in Granada (Spain). Eight dimensions were analyzed, and discriminant function analysis was performed for each vertebra to obtain discriminating functions and study the percentage of correct assignations of these functions. The percentage accuracy was approximately 80% for both vertebrae, but varied according to the sex, being higher for the 7th cervical in males and higher for the 12th thoracic in females. As reported in other populations, the greatest dimorphism values were found for the length of the inferior surface of the vertebral body and the width and length of the vertebral foramen of the 7th cervical vertebra and for the length of the inferior surface of the vertebral body of the 12th thoracic vertebra.     

Sex Determination from the Second Cervical Vertebra: A Test of Wescott’s Method on a Modern American Sample*

Abstract: Numerous methods for establishing a biological profile exist; however, many of these methods rely on the recovery of several specific bones or on fragile skeletal elements that are sometimes irrecoverable. It is for this reason new methods utilizing other previously under‐documented bones should be established and tested by the forensic anthropological community. This study tests the accuracy of Wescott’s (J Forensic Sci 2000;45(2)) method for determining sex from the second cervical vertebra. Specimens were drawn from the donated skeletal collection curated at the Hamilton County Forensic Center (n = 57) and the William M. Bass Donated Skeletal Collection (n = 243). Both intra‐ and inter‐observer error rates were low and accurate classifications ranged from 78% (females‐Function 1) to 90.6% (males‐Function 5). Of the five functions, Function 4 achieved the highest overall accuracy, with 260 individuals (86.7%) falling into the correct category. Overall, this method is an effective classificatory tool for sex estimation.     

Nonmetric sex determination from the distal and posterior humerus in black and white South Africans

The successful identification of human skeletal remains relies on proven diagnostic techniques for sex determination. This research utilized 608 individuals from South Africa (420 men, 188 women) to conduct a blind nonmetric determination of sex from three features of the distal humerus: olecranon fossa shape, angle of the medial epicondyle, and trochlear extension. A scoring system between males and females was implemented, and the aggregate score of the three features determined the estimated sex of the skeletal element in question. With all features combined, black and white South Africans were categorized successfully as either male or female 75.5% (77% accuracy rate for females, 74% accuracy rate for males). This classification rate is lower than what was found in previous studies, but suggests that characteristics of the distal humerus are still quite valuable when estimating skeletal sex. More research is needed to assess reasons for the differential expression of these traits in different populations and to determine whether the method is nonpopulation specific.