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作者以精浆特异蛋白P30为抗原免疫新西兰白兔、豚鼠和鸡三种实验动物,制备了抗P30血清。用双向琼脂扩散法检测兔和豚鼠的抗 P30血清,其特异性和敏感性均达到目前国外同类产品的水平。抗 P30血清与阴道分泌物,血清、唾液、尿液、初乳以及羊精液、鸡精液均不出现交叉反应。用抗 P30血清检测混合的人精浆,其抗原效价为1:160;P30含量可测到12.5ug/ml。在三种动物的抗血清中,豚鼠抗 P30血清的抗体效价最高。以不同浓度的 P30测豚鼠、兔和鸡抗 P30血清抗体效价豚鼠平均滴度可达52.50,兔次之,鸡的抗 P30血清最差。经作者制备的抗 P30血清可用来确证精液。 相似文献
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Tooth fragments freshly extracted from humans and rats were stored at either 4 degrees C or room temperature in dry or humid conditions for periods ranging from 1 to 6 months. The fragments were reduced to powder and antigens were extracted. Comparison of these samples was carried out using Counter Current Electrophoresis. Extracted sera were tested against known specific antisera and resultant precipitin reactions stained for examination. Correct species identification was possible both from desiccated and humid fragments but there was species variation in the sensitivity of the method. All the extracts from human teeth were positive against human antisera. In the rat some test specimens were initially negative but became positive following further dilution of the extracts. 相似文献
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急性吗啡中毒大鼠主要器官内吗啡的免疫组化定位研究 总被引:5,自引:1,他引:4
一次静脉注射12.5mg/kg。bw的盐酸吗啡染毒雄性S-D大鼠,2小时后处死,取其脑、肾、肝、肺、心组织以2%戊二醛和4%多聚甲醛混合液固定后,常规石蜡切片。运用抗吗啡抗血清及SABC技术染色。结果显示上述组织切片有不同程度的阳性染色,阳性着色主要见于肾髓质部分肾小管上皮细胞,肝脏中央静脉周围的肝细胞、肺泡上皮细胞及肺内小支气管粘膜上皮细胞、中脑部分神经细胞、室管膜细胞、心肌细胞.以及各器官小血管及毛细血管内皮细胞胞浆、血浆及肾小管腔内尿液。 相似文献
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挤压伤大鼠早期心脏损伤的细胞机制 总被引:3,自引:1,他引:2
目的观察挤压伤大鼠血清对培养的新生大鼠心肌细胞的某些作用,拟阐明挤压伤早期心肌细胞损伤的细胞机制。方法培养1~3d龄SD大鼠心肌细胞,观察挤压伤大鼠血清对心肌细胞搏动频率、表面积、蛋白质含量、3H-亮氨酸掺入、胞内钙浓度和Fos蛋白表达的影响。结果与正常大鼠血清组比较,挤压伤大鼠血清培养的心肌细胞搏动频率(次/min)由88.3±20.6降为26.4±16.7,心肌细胞表面积、蛋白质含量、3H-Leu掺入、胞内游离钙浓度(nmol/L)和Fos蛋白表达阳性指数增加。结论挤压伤大鼠血清通过抑制细胞搏动,增加胞内钙浓度诱导Fos蛋白的表达,引起心肌细胞肥大,介导挤压伤早期的心脏损伤。 相似文献
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自制与商业化抗人精液蛋白P30金标试剂条的法医学应用比较 总被引:1,自引:0,他引:1
目的 检测比较自制和商业销售的抗人精液蛋白P30 金标试剂条 ,探讨它们法医学应用的可靠性。方法 以人精液和人前列腺特异蛋白检测效价及灵敏度 ,以人阴道分泌物、人血清、人唾液和人初乳检测特异性 ,以保存不同年限的陈旧人精斑和斑痕类检材测定检验实际检材的能力。结果 自制抗人精液蛋白P30 金标试剂条效价及灵敏度与商业销售的试剂条中较好的基本相当 ,特异性则相当或好于商业试剂条 ,对非特异性测定均为阴性 ,而商业化试剂条则多有非特异性反应。结论 使用商业金标试剂条 ,要确定其是否合乎法医学检验标准与要求。 相似文献
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The highest sensitivity of the absorption-elution test for detection of A, B, and H antigens in human excretion traces was observed when the excretion traces were treated with protease C and lidase (hyaluronidase) and standard erythrocytes for analysis of the resultant eluates were treated with protease C. Cross-reactions of sera with antigens of contralateral specificity were ruled out by simple dilution of the sera; the reliability of antigen detection was not lost due to pronounced increase in the sensitivity of modified absorption-elution test. 相似文献
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Kolokolova GP 《Sudebno-meditsinskaia ekspertiza》2006,49(4):32-34
Experimental spots of the saliva, sperm, vaginal secretion from persons with groups Ase, Bse, ABSe and Abse were studied with mixed agglutination reaction (MAR) using hemagglutinating sera anti-A and anti-B (heteroimmune, isoimmune), monoclonal antibodies. MAR with monoclonal antibodies was able to diagnose ABSe group not only in the spots of saliva, but also in the spots of sperm and vaginal secretion where heteroimmune sera failed. 相似文献
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The localization of A and B antigens in the organs of blood group AB individuals has been studied using a double immunoenzymatic labeling method. Both A and B antigens were found in the various epithelial cells of these organs, but the epithelial cells could be classified into the following four types depending on the reaction pattern with anti-A and anti-B sera: type 1: cells that stained positive with both anti-A and anti-B sera; type 2: cells that stained positive with anti-A serum only; type 3: cells that stained positive with anti-B serum only; type 4: cells that were negative with both sera. The distribution of each of these epithelial cell types varied considerably, even in the same tissue and individual. Our results seem to suggest that a dissociation in the conversion to the A and B antigens occurs in the tissue of individuals belonging to blood group AB and that the degree of this dissociation varies from tissue to tissue and from cell to cell. 相似文献
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Kolokolva GP 《Sudebno-meditsinskaia ekspertiza》2000,43(4):22-24
Wide spectrum (polyspecific) antiglobulin sera were obtained by rabbit immunization with 10% solution of zymosan-charged globulin (product of yeast digestion with trypsin) with complete Freund's adjuvant. The resultant sera meet the international standards and can be used for forensic medical identification of serum G1m(1) group and for detecting incomplete antierythrocytic complement-fixing anti-Daffi, anti-Kell, and other antibodies in blood transfusion service. 相似文献
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利用进口抗Le~a、Le~b血清筛选OLe(a+b-)和OLe(a-b+)型人,测定其唾液中Le~a和Le~b型物质含量,择其型物质含量高者唾液,用家兔和山羊免疫,合格后采全血,分离血清,用O、A、B型红细胞吸收、除去种属及α和β等凝集素。再用O型Le非相应型红细胞吸收,精制出含不完全抗体的抗Le~a和抗Le~b血清。该血清对Le相应型红细胞均产生明显凝集反应,而不发生非特异的交叉凝集反应。经过盲测鉴定,17份红细胞的Lewis型测定完全准确。制备的抗Le~a和抗Le~b血清在效价和特异性方面达到了引进的同种血清水平,填补了我国抗Le~a、抗Le~b血清制造的空白。 相似文献
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A modification of quantitative absorption and absorption elution tests with blood stain washing before the absorption phase is presented. Due to washing, the effect of carrier object on anti-Le(a) and anti-Le(b) sera is decreased and the sensitivity of the method is increased. Additional adsorption of the sera and elution into test erythrocytes treated with protease C is suggested for increasing the number of standard sera fit for the absorption-elution test. A new technology for preparing anti-Le(a) and anti-Le(b) immunoreagents is described. 相似文献
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A solid-phase ELISA for Gm typing is described. A mixture of anti-Gm serum (or monoclonal anti-Gm antibody) and test serum was incubated in microtiter wells coated with IgG or its fragments of appropriate Gm type. After washing of the wells, the bound antibody was detected with peroxidase-labeled second antibody. The Glm(3), G3m(16), and G3m(21) antigens could be identified by this technique. Since some of the human anti-Gm sera and anti-Rh0 sera required for the conventional hemagglutination-inhibition method are hard to obtain, the ELISA system using anti-Gm antibodies and no anti-Rh0 sera may serve as an alternative to the conventional method. 相似文献