A systematic approach to improve downstream single-cell analysis for the DEPArray™ technology

Most commercially available STR amplification kits have never been fully validated for low template DNA analysis, highlighting the need for testing different PCR kits and conditions for improving single-cell profiling. Here, current strategies rely mainly on adjusting PCR cycle number and analytical threshold settings, with a strong preference for using 30 amplification cycles and thresholds at 30–150 RFU for allele detection. This study aimed to (1) determine appropriate conditions for obtaining informative profiles utilizing a dilution series, and (2) test the outcome on single cells using the DEPArray™ technology. Four routinely applied forensic STR kits were compared by using three different amplification volumes and DNA dilutions down to 3.0 pg, while two well-performing kits were used for single/pooled leucocyte and sperm cell genotyping. Besides reduced costs, the results demonstrate that a 50%–75% PCR volume reduction was beneficial for peak height evaluation. However, this was counteracted by an increased artifact generation in diluted DNA volumes. Regarding profile completeness, the advantage of volume reduction was only prominent in samples processed with Fusion 6C. For single and pooled cells, ESIFast and NGMDetect provided a solid basis for consensus profiling regarding locus failure, although locus dropouts were generally observed as stochastic events. Amplification volume of 12.5 μL was confirmed as appropriate in terms of peak heights and stutter frequencies, with increased stutter peaks being the main artifact in single-cell profiles. Limitations associated with these analyses are discussed, providing a solid foundation for further studies on low template DNA.     

The ‘relics of Joan of Arc’: A forensic multidisciplinary analysis

Archaeological remains can provide concrete cases, making it possible to develop, refine or validate medico-legal techniques.In the case of the so-called ‘Joan of Arc's relics’ (a group of bone and archaeological remains known as the ‘Bottle of Chinon’), 14 specialists analysed the samples such as a cadaver X of carbonised aspect: forensic anthropologist, medical examiners, pathologists, geneticists, radiologist, biochemists, palynologists, zoologist and archaeologist. Materials, methods and results of this study are presented here.This study aims to offer an exploitable methodology for the modern medico-legal cases of small quantities of human bones of carbonised aspect.     

A configurational analysis of 44 US mass shootings: 1975–2015

This study identifies the conditions under which civilian shooter events in the United States become mass episodes of killing. Hitherto, researchers have examined many individual-level variables associated with mass shootings including personal deprivation, family problems, mental illness, among others. Apart from a handful of quantitative studies, scholars have yet to provide a comparative scope to the multiplicity of factors that are influential in bringing about a mass murder. Adopting fuzzy-set qualitative comparative analysis, this study analyses (n = 44) civilian acts of violence that took place from 1975 to 2015 in which some shootings resulted in few deaths and others turned into mass bouts of killing. Empirical results reveal that no variables on their own are sufficient or necessary in accounting for the outcome of a mass shooting. Rather, configurations of causal conditions provide sufficient pathways and new insight into the particular circumstances under which a mass shooting is apt to take place in. The most salient of configurations tell us that mass shootings are prominently influenced by the combination of severe/multiple mental illnesses, a large number/high-powered guns and weak state gun law legislation. Another pathway reveals that mass shootings are also influenced by the interaction of radical ideology, severe/multiple mental illnesses, and high-state level gun ownership.     

Hair analysis for drugs of abuse. Hair color and race differentials or systematic differences in drug preferences?

There is currently a debate in the literature on chemical drug analysis concerning the contribution of biophysical attributes associated with specimens and specimen donors to assay outcome. In recent years this debate has focused on hair analysis, but has in the past also been raised in urinalysis interpretation. In this article we examine several aspects of that controversy. First, we present data regarding the effects of hair color on the distribution of positive hair testing results for three drug classes. We compare these results to negative hair samples from comparable donors. This data is derived from head hair from preemployment donors that was classified according to seven visual color categories. We determined the distribution of colors for hair samples devoid of any of three assayed drugs (amphetamines, cocaine, and cannabinoids). Subsequently, this distribution was compared with the distributions for hairs that had tested positive for amphetamines, cocaine or cannabinoids. We examined a total of 2000 randomly selected samples; 500 negative hair samples and 500 positive samples for each of three drugs: cannabinoids, cocaine, and amphetamine. We also evaluated ethnic/racial factors in relation to positive urinalyses for various ethnic/racial groups. We examined approximately 4000 urine specimens from two different groups, each constituting around 2000 specimens. In addition to ethnicity/race and urinalysis outcome, we also examined the relationship between the hair color distributions of urine donors and the corresponding urinalysis results for the three drug classes. We also compared them to drug-negative samples. Our summary impression is that the observed outcome patterns were largely consistent with differences in drug preferences among the various societal groups. There was little evidence of a pattern attributable to hair color bias alone or selective binding of drugs to hair of a particular color. Likewise, there was no discernible pattern associated with race or ethnicity that would lend support to a "race effect" in drug analysis.     

Offenders’ uncoerced false confessions: A new application of statement analysis?

Purpose. Deception detection research has mainly studied denials and distortions given by students. This study examined true and false confessions as told by offenders. It was hypothesized that the statement analytic techniques Criteria‐Based Content Analysis (CBCA) and Reality Monitoring (RM) would discriminate truths and lies. Methods. Truthful and deceptive confessions to crime were given by 30 offenders (both women and men) in a within‐subject design. The participants were in prison at the time of data collection, and told the truth about a crime they had committed and been sentenced for. In addition, they made up a lie about a different crime after a few minutes of preparation. The transcribed statements were scored for CBCA and RM criteria. Results. Results showed that neither total CBCA nor total RM scores differentiated between lies and truths. Some individual CBCA criteria, however, showed differences: more self‐deprecations and doubts about own testimony in the told lies, and more unexpected complications in the truths. Conclusions. The results are discussed in relation to statement analysis of offenders’ accounts, individual CBCA criteria, as well as the development of criminal experience and familiarity with the event and setting. Implications for triers of fact and suggestions for future research are considered.     

Mother–infant separations in prison. A systematic attachment-focused review of the academic and grey literature

This review systematically searched UK academic and grey literature in relation to mother and child separation in prison. Attachment theory is referred to in current prison policy for mothers and could provide a framework linking policy and practice. Reviewing grey literature provided an opportunity to explore practice-based literature. Twenty-four academic papers and 51 grey documents were reviewed. Use of attachment theory in the academic literature varied according to discipline, ranging from extensive use to no use. There was greater use of attachment theory in the grey literature. Despite linguistic differences, all documents highlighted the detrimental impact of separation on imprisoned mothers. However, specificity was lacking regarding support for mothers, and staff needs were overlooked. Given its use across the sparse research and practice literature, and its basis for policy, attachment theory could underpin theoretically informed support for imprisoned mothers separated from their infants and staff who support them.     

Who wants to be an ideal victim? A narrative analysis of crime victims' self-presentation

The aim of this article is to analyse how self-presentation is performed in victim narratives and what possible variant features are present in the narratives. This is examined by means of narrative analyses of face-to-face interviews with crime victims (n = 6). The narratives were grouped into two categories: 1) narratives about the crime victim as an established citizen, and 2) narratives about the crime victim as an outsider. How the narrators presented their status in society was relevant for how they understood the offender, the crime, and Victim Support. Importantly, the interviewed victims did not construe themselves as ‘ideal victims’ as they all projected personal strength in their self-presentations. Furthermore, the victim narratives normalized (rather than idealized) the victim while construing the offender as in need of treatment. The article concludes by discussing future research needs and the role of victim self-presentations for psychological well-being and trust in the criminal justice system.     

A feminist analysis of the American criminal justice system’s response to human trafficking

While criminological and criminal justice scholars have made important contributions to human trafficking literature, to date, such scholarship has been devoid of a feminist analysis of the topic as scholars have spent little time reflecting on how patriarchy impacts the criminal justice system’s response to human trafficking. Such examination is necessary to understand how the issue of patriarchy impacts criminal justice system approaches to the crime as well as the outcomes of such anti-human trafficking efforts. It is argued the influence of patriarchy on criminal justice system policy development and practice undermines the system’s anti-human trafficking efforts.     

GeneMarker® HID: A reliable software tool for the analysis of forensic STR data

Abstract: GeneMarker^® HID was assessed as a software tool for the analysis of forensic short tandem repeat (STR) data and as a resource for analysis of custom STR multiplexes. The software is easy to learn and use, and includes design features that have the potential to reduce user fatigue. To illustrate reliability and accuracy, STR data from both single‐source and mixture profiles were analyzed and compared to profiles interpreted with another software package. A total of 1898 STR profiles representing 28,470 loci and more than 42,000 alleles were analyzed with 100% concordance. GeneMarker HID was also used to successfully analyze data generated from a custom STR multiplex, with simplified and rapid implementation. Finally, the impact of the user‐friendly design features of the software was assessed through a time scale study. The results suggest that laboratories can reduce the time required for data analysis by at least 25% when using GeneMarker HID.     

Ready or not? A systematic review of case studies using data-driven approaches to detect real-world antitrust violations

Cartels and other anti-competitive behaviour by companies have a tremendously negative impact on the economy and, ultimately, on consumers. To detect such anti-competitive behaviour, competition authorities need reliable tools. Recently, new data-driven approaches have started to emerge in the area of computational antitrust that can complement already established tools, such as leniency programs. Our systematic review of case studies shows how data-driven approaches can be used to detect real-world antitrust violations. Relying on statistical analysis or machine learning, ever more sophisticated methods have been developed and applied to real-world scenarios to identify whether an antitrust infringement has taken place. Our review suggests that the approaches already applied in case studies have become more complex and more sophisticated over time, and may also be transferrable to further types of cases. While computational tools may not yet be ready to take over antitrust enforcement, they are ready to be employed more fully.     

A qualitative analysis of nonoffending partners’ experiences and perceptions following a psychoeducational group intervention

The present research is a qualitative study analysing therapeutic letters written by the nonoffending partners of men who have perpetrated child sexual abuse, after completion of a psychoeducational group intervention. It sought to identify themes relating to their experiences post-intervention. Findings indicated that the intervention contributed to women having increased insight into sexual offending, and feeling more confident and empowered. In addition, they displayed an awareness of risk issues, and an intention to play a role in helping to manage their partner’s future risk. Implications for research and clinical practice are discussed.     

Does victimization reduce self-control? A longitudinal analysis

Purpose

To examine the effect of victimization on self-control.

Method

Five waves of data from the GREAT survey are analyzed; the effect of prior victimization on subsequent self-control is estimated using the dynamic panel generalized-method of moments.

Results

Victimization reduces subsequent self-control in the near term.

Conclusions

The findings point to another source of low self-control, help to explain why prior victimization is linked to subsequent victimization, and provide support for general strain theory - which predicts that strains such as victimization will reduce self-control.     

A snapshot of scja members: An analysis of paper presentations at the 1998–2003 scja annual meetings

This paper contains an analysis of papers scheduled for presentation at the annual meetings of the Southern Criminal Justice Association during a six-year period (1998–2003). The goal is to discover which research topics are most popular and which are least popular in order to develop a clearer picture of what the SCJA is, what its members value, what they stand for, and what they think is important.     

Does neighborhood watch reduce crime? A systematic review and meta-analysis

Neighborhood watch grew out of a movement in the USA during the late 1960s that promoted greater involvement of citizens in the prevention of crime. Recent estimates suggest that over a quarter of the UK population and over 40% of the US population live in areas covered by neighborhood watch schemes. The current paper presents the results of a recent systematic review of evaluations of neighborhood watch. The main findings of the narrative review were that about half of the schemes evaluated showed that neighborhood watch was effective in reducing crime, with most of the other evaluations having uncertain effects. The main findings of the meta-analysis were that 15 of the 18 studies provided evidence that neighborhood watch reduced crime. While the results of the review are encouraging, it was concluded that more high-quality research needs to be done to help explain why study variations exist.