The need for a complete dental autopsy of unidentified edentulous human remains

In December 2017, a decomposed unidentified body was found near the river Tronto in Teramo, Italy. The corpse was found without any identifying documents or specific personal belongings, except for a packet of cigarettes. The medical examiner determined a gastric perforation from the intake of hydrochloric acid to be the cause of death. A jar of muriatic acid found near the body led to suicide being considered the manner of death. The Penal Court in Teramo appointed two forensic odontologists to complete the postmortem assessment and collect dental data for personal identification. The corpse was found wearing a complete set of upper and lower dentures. The dental autopsy and 42 periapical X-ray images helped generate a biological profile of a man totally edentulous with upper and lower dentures, as well as an osteosynthesis with two plates and screws in the left ascending ramus of the mandible. In March 2018, the sister of a missing person reported the disappearance of her brother, and a presumptive identification was performed through visual recognition of the decomposed body. The sister confirmed the presence of two dentures and the location of the maxillo-facial surgery for the treatment of the fractured mandible. A complete dental autopsy was able to establish his identity without any DNA comparison needed. This case highlights the importance of performing a complete dental autopsy inclusive of dental radiographs, and its value in the identification of all unknown human remains even when totally edentulous.A complete dental autopsy should be performed in all cases of human identification.

Keypoints

• Despite a corpse being edentulous, a complete dental autopsy can still be useful.
• Dental radiographs, such as bitewings, periapical images, panoramic radiographs, and CT scans, are recommended in all identification autopsies.

     

Forensic aspects of aboriginal skeletal remains in Australia

The assessment of Aboriginal skeletal remains and their distinction from whites is an area of major importance to the Australian forensic pathologist. The Aborigines, the indigenous people of Australia, are a distinct racial group with many characteristic anthropological features. The assessment of race is best made from an examination of cranial traits, 20 which are of value. A characteristic pattern of attrition of the teeth provides a method for establishing a skeleton as pre-European contact Aboriginal or postcontact tribal Aboriginal. The limb proportions of Aborigines differ significantly from other races and provide a useful adjunct to other racial discriminants. Quantifiable sex discriminants in the Aboriginal pelvis and femur differ in their ranges of values from other races. The humerus and shoulder girdle are of no value in making a racial distinction and are of limited value in sexing Aboriginal skeletons. Some pathological changes seen in Aboriginal skeletons are of value in corroborating race. These pathological changes include evulsion of incisor teeth, healed "parry fractures" of the ulnae, tibial squatting facets, treponemal changes, and auditory exostoses. The place and manner of burial together with related artefacts, features, and ecofacts may also provide corroboration.     

DNA extraction and sex determination of human skeletal remains

DNA was extracted from human bones remained for 3 to 15 years by using the method developed in our laboratory. A pair of X-Y homologous primer were used to amplify the specific DNA fragments of Amelogenin gene. All the 35 samples yielded correct results. Our method is rapid, sensitive and reliable for the sex determination of skeletal remains.     

Stress deformations of skeletal remains from prehistoric cremation burials

Vertebrae and parts of joints from long bones from prehistoric cremations of the Late Bronze Age and Early Iron Age are presented, showing marked deformations caused by pressure during cremation when bones exposed to temperatures of 400 degrees-500 degrees Centigrade display minimal hardness. The vertebrae with deformation of the arcus parts are only from the lower vertebral column; on account of the weight of this body region, this suggests that the corpse lay in the dorsal position at the place of cremation. The fact that there were deformed arches only on one side might suggest an irregular structure of the surface on which the corpse lay. In the prone position, i.e. in the absence of pressure caused by body weight, all vertebrae should be unaltered; alternatively, if cremation wood was piled on top of the corpse, the arches of thoracic and cervical vertebrae are also likely to be deformed. Deformations resulting from weight bearing were found in the joints of extremities only, with flattening of the heads of joints as well as fissures in the external layers of bones and compression into folds. Some bones show distinct even grooves caused by direct pressure, possibly resulting from the fixation of joint regions for cremation? Evidently the deformations caused by the weighting down of different skeletal regions provide hints that may help in the recognition of cremation rites. It would be helpful if experimental cremations could be performed to establish the pressure necessary to cause deformations.     

Processing of skeletal remains. A medical examiner's perspective

A multidisciplinary approach to the investigation of skeletal remains is presented. Guidelines for recovery, documentation, examination, processing, storage, and release of skeletal material are offered and discussed.     

Field recovery and analysis of horse skeletal remains.

In response to a request from an insurance company investigating a claim relative to the death of four race and show horses, allegedly as a result of deliberate starvation, physical anthropologists of the Louisiana State University (LSU) Department of Geography and Anthropology undertook the recovery and analysis of skeletal remains said to be those of the animals. The objectives were to determine the number and kinds of animals represented, their ages, and sexes, and, through morphometric evaluation of bone density at the LSU School of Veterinary Medicine, whether there was evidence of nutritional osteodystrophy. The skeletons were the remains of four horses. In comparisons of data derived from these skeletons with breeding records for the horses described in the insurance claim, it was established that the skeletons were those of mares, as were the insured animals, and that the relative ages of the four approximated those of the insured horses. The skeletal samples submitted for morphometric evaluation showed no evidence of nutritional osteodystrophy and, thus, provided no support for the contention that death had resulted from starvation. In one horse, the superior aspect of the right ascending ramus of the lower jaw below the coronoid process revealed a gunshot wound; the other skeletons showed no evidence of trauma.     

Microscopical study on estimation of time since death in skeletal remains

For the purpose of estimating time since death in skeletal remains, postmortem changes in human compact bones were examined by microradiography and electron microscopy. The UV-fluorescence of the peripheral zone of compact bone was also examined by microscopic spectrophotometry. Microradiographic examination revealed no morphological changes in bones left in the open air for long periods, except one of 15 years since death. In bones left in the soil, vacuoles of 5-10 microns diameter, which contained a honeycomb-like structure formed by small vacuoles of 0.5-1 microns diameter, were found in the peripheral zone of the substantia compacta approximately 5 years since death, and in bones of 6 years or more, this change extended to the mid-zone. In bones left in the sea for 4-5 years, vacuoles of 5-10 microns diameter were observed in the outer peripheral zone of the substantia compacta. The relative intensity of UV-fluorescence in bones dwindled with time since death and the correlation coefficient was considerably high.     

The forensic evaluation of burned skeletal remains: A synthesis

In recent years, research and case experience have greatly augmented knowledge regarding the effects of extreme heat on skeletal remains. As a result of this effort, enhanced interpretation is now possible on such issues as the extent of recovery, reconstruction, trauma, individual identification, size reduction, thermal effects on histological structures, color variation, the determination if remains were burned with or without soft tissue, DNA recovery and residual weight. The rapidly growing literature in this area of forensic science includes experimental research that elucidates the dynamics of the thermal impact on skeletal structure and morphology.     

Positive identification of American Indian skeletal remains from radiograph comparison

The case reported here documents how comparative data gleaned from study of museum collections enabled positive identification of an American Indian homicide victim through radiograph comparison. In particular, the shape of the lateral border of the scapula proved to be a unique anatomical feature, appropriate for establishing positive identification.     

Genetic analysis of the skeletal remains attributed to Francesco Petrarca

We report on the mitochondrial DNA (mtDNA) analysis of the supposed remains of Francesco Petrarca exhumed in November 2003, from the S. Maria Assunta church, in Arquà Padua (Italy) where he died in 1374. The optimal preservation of the remains allowed the retrieval of sufficient mtDNA for genetic analysis. DNA was extracted from a rib and a tooth and mtDNA sequences were determined in multiple clones using the strictest criteria currently available for validation of ancient DNA sequences, including independent replication. MtDNA sequences from the tooth and rib were not identical, suggesting that they belonged to different individuals. Indeed, molecular gender determination showed that the postcranial remains belonged to a male while the skull belonged to a female. Historical records indicated that the remains were violated in 1630, possibly by thieves. These results are consistent with morphological investigations and confirm the importance of integrating molecular and morphological approaches in investigating historical remains.     

The cemetery skeleton. An exercise in the identity of skeletal remains

In this brief case report, we describe the several means used in 1982 to identify the skeletal remains of a person who disappeared In August 1964. This report exemplifies the multidisciplinary, cooperative approach required for a successful result in such cases.     

An esoteric technique useful in the identification of unidentified remains from the examination of faded,illegible hospital identification wristbands

A technique routinely used in the examination of questioned documents has been found to be of assistance when employed in the examination of faded and/or partially legible hospital identification wristbands found with unidentified remains (UIDs). A non-destructive test used predominately by forensic document examiners in the analysis of writing inks, handwritten alterations, and obliterations has proven useful throughout the years when confronted with this unusual type of documentary evidence. This discussion paper was prompted by the Tri-State Crematory disaster, Walker County, Georgia, from a request by investigators as to whether or not any information could be obtained from the examination of faded hospital identification wristbands where no information was readily discernable. Subsequent analysis by non-destructive infrared inspection, a standard technique used in the examination of questioned documents, proved useful in assisting with the identification of unidentified skeletal remains.     

Analysis of pathological and non-pathological human skeletal remains by FT-IR spectroscopy

In this study, we report the chemical analyses of various non-pathological, tuberculosis and syphilis infected bone samples from different burial environments by Fourier transform infrared spectroscopy (FT-IR), in the framework of a general study of diagenesis. Dating human skeletal remains is one of the most important and yet unreliable aspects of forensic anthropology. In this paper, a new method has been suggested, using the crystallinity index and carbonate-phosphate index as a means of distinction between recent and archaeological, anthropological bone samples. Pathological bone samples were analyzed with the same method to see if changes in crystallinity interfere with the process of dating.     

Radiocarbon dating as tool to assist in triaging cases of unidentified human remains in Victoria,Australia: A case series

In cases where human remains are unidentified because there is no initial identification hypothesis, limited contextual information, and/or poor preservation, radiocarbon (¹⁴C) dating may be a useful tool to further assist with identification. Through measuring the amount of ¹⁴C remaining in organic material, such as bone, teeth, nail, or hair, radiocarbon dating may provide an estimated year of birth and year of death for a deceased person. This information, may assist in, establishing whether a case of unidentified human remains (UHR) is actually of medicolegal significance and therefore, requires forensic investigation and identification. This case series highlights the application of ¹⁴C dating to seven of the 132 UHR cases in Victoria, Australia. Cortical bone was sampled from each case and the level of ¹⁴C was measured to provide an estimated year of death. Four of the seven cases analyzed contained the levels of ¹⁴C consistent with an archeological timeframe, one contained a level of ¹⁴C consistent with a modern (i.e., of medicolegal significance) timeframe, and the results for the remaining two samples were inconclusive. Applying this technique not only reduced the number of UHR cases in Victoria but also has investigative, cultural, and practical implications for medicolegal casework in general.