mtDNA—HVⅠ和细胞色素b片段的复合扩增及其法医学应用

目的探讨复合扩增mtDNA D环HV I和细胞色素b片段进行种属鉴定和个体识别的方法及mtDNA-HV I多态性。方法用两对引物同步扩增HV I片段与细胞色素b片段,银染显带检测扩增产物,ABI377测序仪及荧光测序技术分析扩增产物序列多态性。结果人类有279bp,358bp两条带,动物只有358bp一条带。通过对131例随机广东汉族人群个体进行mtDNA控制区(15997～16236))序列测定统计,得出此区域的序列多态性。共发现69个位点变异,平均每个个体存在2.679个碱基突变,检出67个单倍型,基因多样性为97.92％。结论mtDNA控制区(15997—16236)具有较高的序列多态性。为良好的个体识别标记。复合扩增mtDNA D环HV I与细胞色素b片段进行测序分析可以同步进行种属鉴定和个体识别。     

鉴定与司法鉴定概念的引入及其演进研究

本文结合史料,对汉语和我国法律中的“鉴定”与“司法鉴定”两个词语的来源与演变进行了回顾和分析。同时,还对鉴定一词当初引入法律和司法鉴定法律界定产生的历史和现实意义进行了初步阐述。     

Psychedelic fungus (Psilocybe sp.) authentication in a case of illegal drug traffic: sporological,molecular analysis and identification of the psychoactive substance

In nature, there are >200 species of fungi with hallucinogenic properties. These fungi are classified as Psilocybe, Gymnopilus, and Panaeolus which contain active principles with hallucinogenic properties such as ibotenic acid, psilocybin, psilocin, or baeocystin. In Chile, fungi seizures are mainly of mature specimens or spores. However, clandestine laboratories have been found that process fungus samples at the mycelium stage. In this transient stage of growth (mycelium), traditional taxonomic identification is not feasible, making it necessary to develop a new method of study.Currently, DNA analysis is the only reliable method that can be used as an identification tool for the purposes of supporting evidence, due to the high variability of DNA between species. One way to identify the species of a distinctive DNA fragment is to study PCR products analyzed by real time PCR and sequencing. One of the most popular sequencing methods of forensic interest at the generic and intra-generic levels in plants is internal transcribed spacer (ITS). With real time PCR it is possible to distinguish PCR products by differential analysis of their melting temperature (Tm) curves.This paper describes morphological, chemical, and genetic analysis of mycelia of psychedelic fungi collected from a clandestine laboratory. The fungus species were identified using scanning electron microscopy (SEM), mass spectrometry, HRM analysis, and ITS sequencing. The sporological studies showed a generally smooth surface and oval shape, with maximum length 10.1?μm and width 6.4?μm. The alkaloid Psilocyn was identified by mass spectrometry, while HRM analysis and ITS sequencing identified the species as Psilocybe cubensis. A genetic match was confirmed between the HRM curves obtained from the mycelia (evidence) and biological tissue extracted from the fruiting bodies. Mycelia recovered from the evidence and fruiting bodies (control) were genetically indistinguishable.     

From unknown to known: Identification of the remains at the mausoleum of fosse Ardeatine

During the Second World War, on 24th March 1944, 335 Italians were massacred near Rome by the occupying forces of Nazi Germany. Four months later forensic examination led to the identification of 323 out of 335 victims. After approximately 60?years, the identification of the remaining unidentified twelve victims began with anthropological and genetic analysis carried out by a team of Italian forensic experts. Anthropological analysis was performed in field in order to confirm the sex of each victim and verify the presence of only one individual in each grave for a correct sampling. Selected bone fragments for each individual were then collected and transferred to the laboratory for genetic analysis. Although the anthropological ante mortem information was limited, morphological and metrical data was collected for a possible future identification of the victims. Subsequently, the typing of autosomal loci, Y-STR and mtDNA D-loop region of all bone and available reference samples was conducted. LR and cumulative LRs obtained from autosomal STR and Y-STR results confirmed the alleged relationship between three victims and their relatives with values over 10⁴ (one sample) and 10⁶ (two samples). Therefore, the genetic analysis offered the families the possibility of replacing the number of the grave with the name of the victim.     

Narco-Terrorism: The Merger of the War on Drugs and the War on Terror

The aim of this article is to analyse the phenomena of narco-terrorism and the practical measures utilised to counter this threat. By adopting the model of the crime-terror continuum developed by Tamara Makarenko, the article will outline the similarities and dissimilarities of narcotics trafficking and terrorism in order to provide a more nuanced perspective on the concept of narco-terrorism. By doing so, the article will evaluate the kind of approach taken in combating the threat of narco-terrorism.     

The efficiency of Y-chromosome markers in forensic trace analysis and their inclusion in the Austrian National DNA Database

Y-STR markers are a valuable tool in the analysis of biological traces in which a mixture of male and female trace material is to be expected. It is possible to generate a Y-chromosome DNA profile, even if all the prior sperm tests are negative and no sign of any male component is found in amelogenin. In 38 of a total of 239 sexual offences a perpetrator trace was identified solely using Y-STR analysis. Based on these findings, the Austrian National DNA Database was expanded to include Y-STRs in 2012 with the primary objective to identify serial sexual offences.     

Forensic anthropology in the global investigation of humanitarian and human rights abuse: Perspective from the published record

Forensic anthropologists have played key roles in the historical development of forensic science applications to global humanitarian and human rights issues. These anthropological initiatives can be traced back to the Smithsonian seminar organized by T. D. Stewart in 1968 and published in 1970. Key developments include the 1984 delegation sent by the American Association for the Advancement of Science to Argentina and the formation of the Argentine Forensic Anthropology Team. Subsequent highlights include major anthropological involvement in support of investigations by international criminal tribunals, formation of forensic anthropology teams in different countries and activities of the International Commission of Missing Persons and the forensic unit of the International Committee of the Red Cross. Recent developments feature the formation of the Humanitarian and Human Rights Resource Center of the American Academy of Forensic Sciences and its support of worthwhile projects in many countries. The published record provides historical perspective on these developments.     

Watch for those fragments of evidence: the use of an automatic timepiece to help correlate a helicopter crash site from the Vietnam War

This case study illustrates the use of the date function on an automatic wristwatch to help identify a Vietnam War helicopter crash site. The location of a crash incident can sometimes be uncertain because of inadequate or inaccurate wartime records and the passage of time. Artifacts recovered from a prospective crash scene are regularly used to correlate the loss incident. In this case study, a recovered automatic watch displayed a date 2 days later than the reported loss incident. Although the date conflicts with the aircraft crash incident report, it is observed that a fully wound automatic watch continues to work for c. 2 days after movement of the watch ceases. Thus, the watch's date in fact correlates with the aircraft crash incident report. It is noted that automatic watches may also be used to date scenes of crime.     

A new strategy for the discrimination of mitochondrial DNA haplogroups in Han population

Mitochondrial DNA (mtDNA) haplogroup discrimination is interesting not only for phylogenetic and clinical but also for forensic studies. We discriminated the mtDNA haplogroups of 570 healthy unrelated Han people from Zhejiang Province, Southeast China, by comprehensive analysis mutations of the hypervariable segments-I sequence and diagnostic polymorphisms in mtDNA coding region using real-time polymerase chain reaction (RT-PCR), which was compared with the widely used PCR and restriction fragment length polymorphism (PCR-RFLP) method. The results showed that in superhaplogroup M, haplogroup D was the most common haplotype within this assay to 24.6%, and in the other superhaplotype N, haplogroup B and F were the most common groups. Samples re-identified by PCR-RFLP showed the consistent results that were got with RT-PCR. In conclusion, the RT-PCR strategy appears to be an accurate, reproducible, and sensitive technique for the discrimination of mtDNA haplogroups, especially for mass screenings quickly and economically.     

宪法危机理论:以美国内战与重建时期为例的阐释

宪法危机的发生有两个条件 :第一 ,在根本性问题上 ,宪法条文具有强烈的容易引起宪法解释争端的模糊性 ;同时 ,这些模糊的宪法条文所规定的问题 ,在现实政治生活中又已经发展成为实际的、主要的政治问题。我通过对美国内战与重建时期的历史检视 ,发现在美国联邦宪法中存在着三个符合这些条件、并最终引发宪法危机的模糊点 :联邦制度和最高主权归属的问题、共和制度与奴隶制度的关系问题、公民身份与公民权利的问题。这三个问题也是美国内战与重建时期要着力解决并通过暴力手段得以解决的问题。     

The NucleoSpin DNA Clean-up XS kit for the concentration and purification of genomic DNA extracts: An alternative to microdialysis filtration

Traditionally, DNA extracts from biological evidence items have been concentrated and rinsed using microdialysis filtration units, including the Centricon^® and Microcon^® centrifugal filter devices. As an alternative to microdialysis filtration, we present an optimized method for using NucleoSpin^® XS silica columns to concentrate and clean-up aqueous extracts from the organic extraction of DNA from biological samples. The method can be used with standard organic extraction and dithiothreitol (DTT)-based differential extraction methods with no modifications to these methods prior to the concentration and clean-up step. Extracts from laboratory-prepared bloodstains, saliva and semen stains have been successfully amplified with both qPCR and STR assays. Finally, the total time to process a set of samples with the NucleoSpin^® XS column is approximately 30 min vs. approximately 1.5 h with the Centricon^® YM-100 filter device.     

Two arch criteria of the ilium for sex determination of immature skeletal remains: A test of their accuracy and an assessment of intra- and inter-observer error

Although the assignment of sex to immature skeletal remains is considered problematic, some traits have been considered useful for both forensic and bioarchaeological applications. One such trait is the arch criterion found in subadult ilia, which is defined relative to the greater sciatic notch-auricular surface area. In adults, the composite arch has also been described in relation to this area and has proven relatively successful in sex determination. This study offers an examination of the accuracy of the arch criterion and the composite arch in determining the sex of subadult skeletal remains, and an assessment of intra- and inter-observer scoring error. A sample of 97 skeletons of known sex and age (<15 years) from the Lisbon collection (Portugal) were selected and the traits were scored by three observers on orthogonal photos of each ilium. In general the agreement within (67.7–88.5%) and between (50.5–76.3%) examiners was poor and overall accuracy (26.7–52.6%) did not meet the expectations of that reported in previous studies. The authors suggest that this derives from great variation in morphology, difficulties in interpreting criteria and possibly a lack of association between the expression of the traits and sex. Careful examination of sex-related morphology in the immature skeleton and additional blind tests of so-called useful traits should continue to be carried out.     

Inference structures for crime analysis and intelligence: the example of burglary using forensic science data

There has been much work dedicated to crime analysis and intelligence in recent times. Independently, physical evidence has shown great potential for linking crimes and bringing solid informative data through the increased use of multiple databases. However, their informative potential is still often underestimated and has been poorly integrated into police information systems. We propose a framework that fully introduces this data into an intelligence based system. This framework is built on the study of inference structures extracted from investigators’ every day implicit reasoning processes. Five specific inferences are studied with the particular problem of serial burglary investigation across independent police and legal structures. On the basis of such an analytical approach, a computer prototype has been designed; it has shown great promise and has resulted in several operational successes.     

Pericardial lipoma: an autopsy case and review of the literature

Postmortem examination performed on a 70-year-old man revealed a thinly encapsulated, ovoid, large mass in the right hemithorax. The tumor was attached with its pedicle to the atrioventral pericardium and adjoined the diaphragm with compression of the right lower lobe of the lung. There was no adhesion between the tumor and the surrounding organs. Light microscopy showed a lipoma consisting of mature adipose tissue, and no malignancy was found.